Earticle

한국인삼(韓國人蔘)의 뿌리중(中)의 ether가용성분중(可溶成分中)에서 불령화물(不齡化物)을 검출(檢出)한다음에 Thinlayer chomatography법(法)과 Gas liguid Chromatography법(法)에 의해서 Sterol의 성분(成分)을 검출(檢出)하였다. 그 결과인삼중(結果人蔘中)에서 stigmasterol이 반치폭법(半値幅法)에 의하면 15.39%. 그리고 planimetry법(法)에 의하면 13.92%이었고 β-Sitosterol은 반치폭법(半値幅法)에 의하면 77.87% 그리고 planimetry법(法)에 의하면 82.72%로 가장 함량(含量)이 높았고 Campesterol은 반치폭법(半値幅法)에서는 6.74% 그리고 Planimetry법(法)에서는 4.36%가 됨을 알수 있다.

Korean ginseng sterols were obtained from the non-saponifiable matters of etherial extract of the root. The composition of sterols have been determined by gas liquid chromatography and thin-layer chromatographic analysis. The results showed that contents of sterols were β-Sitosterol, 77.87% by method of triangulation and 82.72% by methode of planimetry and stigmasterol,15.39% by methode of triangulation and 13.92% by methode of planimetry and campesterol, 6.74% by method of triangulation and 4.36% by methode of planimetry.

오징어의 동부근육(胴部筋肉)을 각종방법(各種方法)으로 처리(處理)하여 cross section하고 Van Gieson방법(方法)으로 염색(染色)하여 박막상(薄膜狀)의 표피층(表皮層)이 박리(剝離)되는 상태(狀態)를 현미경으로 관찰한 결과(結果)는 다음과 같다. 1. 일반적으로 사용되는 온탕법(溫湯法)에서 1, 2, 3층(層)은 제거(除去)되지만 4층(層)은 거의 제거(除去)되지 않았다. 2. 열탕처리(熱湯處理)에 의해서 근섬유(筋纖維) 조직(組織)에 근섬유(筋纖維)의 방향(方向)으로 많은 균열 현상을 보였다. 3. 초산소오다가 질산소다. 황산(黃酸)소오다 및 주석산(酒石酸)소오다 처리(處理)보다 박리상태(剝離狀態)가 현저히 우수하였으며 특(特)히 고농도(高濃度)에서 현저하고 약품처리(藥品處理) 방법중(方法中) 가장 우수한 효과(?果)를 보였다. 4. 단백(蛋白) 분해효소(分解酵素) 처리시(處理時) 표피(表皮)의 1, 2, 3, 4, 층(層)은 완전히 제거(除去)되며 특(特)히 표피(表皮)와 육(肉)과의 경계면 섬유(纖維)를 현저하게 팽윤(澎潤)시켰다. 5. 효소(酵素)와 초산소오다로 처리(處理)하는 2종류(種類)의 박피법(剝皮法)은 유효(有效)하다는 것을 확인(確認)하였다.

Treated with various methods, body muscle of squid was cross section, stained by Van Gieson staining method, and observed microscopically to study on the state of thin layer of skin separated from meat. Results obtained were summarized as follows : 1. By general hot spring treatment were 1st. 2nd. and 3rd. layers of skin removed from squid muscle but 4th. not. 2. By boiling treatment was tissue of muscle fibre fissured largely toward the muscle fibre. 3. Sodium acetate treatment was superior to sodium nitrate, sodium sulfate and sodium tartrate treatment in the effect that the skin was separated from meat. Especially, concentrate solution of sodium acetate had the most excellent effect in the chemical reagent treatment. 4. By proteolytic enzyme treatment were 1st. 2nd. 3rd. and 4th. layers of skin removed all from squid muscle and the boundary fibre between skin and meat swelled in particular. 5. Two kinds of skin removing method, proteolytic enzyme treatment and sodium acetate treatment, were desirable to the actual processing.

생선(生鮮)으로 부터 생선(生鮮) 냄새를 효과적(效果的)으로 제거(除去)하기 위한 기초작업(基礎作業)으로서 ethanol과 물을 추출용매(抽出溶媒)로 사용(使用)하여 꽁치로 부터 생선(生鮮) 냄새의 주성분(主成分)인 trimethylamine과 변패(變敗)의 원인(原因)이 되며 직접간접(直接間接)으로 불쾌취(不快臭)를 조성(造成)하는 지방(脂肪)을 제거(除去)할 목적(目的)으로 실험(實驗)하였던 바 다음과 같은 결과(結果)를 얻었다. 1. 꽁치중(中)의 trimethylamine은 추출시(抽出時)의 ethanol의 최종농도(最終濃度)가 40~55%수준이었을 때 가장 추출(抽出)이 잘되었다. 2. 꽁치를 boiling absolute ethanol로서 연속추출(連續抽出)을 행(行)할 때 추출(抽出)은 서로 다른 두 단계로 진행(進行)되었다. a) 추출(抽出)의 첫 단계에선 생선(生鮮)의 탈수(脫水)와 동시(同時)에 trimethylamine이 주(主)로 추출(抽出)되었으며 b) 생선(生鮮)이 탈수(脫水)된 그다음 단계에서 지방(脂肪)이 효율적(?率的)으로 추출(抽出)되었다.

For removal of trimethylamine (TMA) which is generally accepted as the main component of marine fish oder and the lipids in marine fish which contribute directly or indirectly to the odor of fish flour, extractions of raw Cololabis saira were carried out with Ethanol-Water Mixture or Absolute Ethanol. Results obtained are as follows: 1. Efficient extraction of TMA from raw Cololabis saira took place when final concentration of ethanol was 40~55%. 2. When successive extraction of raw Cololabis saira was carried out with boiling absolute ethanol, the extraction proceeded in two distinct stages: a) In the first stage, efficient dehydration of raw Cololabis saira and efficient TMA extraction took place simultaneously. b) In the second stage, efficient lipid extraction followed after the dehydration stage.

우리나라의 독특(獨特)한 수산가공품(水産加工品)의 일종(一種)인 굴비의 정미성분(呈味成分)에 대한 기초자료(基礎資料)를 얻기 위하여 참조기, Pseudosciaena manchurica,를 원료(原料)로 하여 굴비가공(加工) 중의 유리아미노산의 변화(變化)를 실험(實驗)하였다. 참조기의 유리아미노산 조성(組成)을 보면, 함량이 많은 것은 glutamic acid, alanine, lysine이고, 다음이 leucine, glycine, serine, threonine이고, 함량이 적은 것은 isoleucine, histidine, aspartic acid이며, proline, valine, methionine, tyrosine, phenylalanine은 함량이 극히 적었다. 굴비가공(加工)중 유리아미노산 조성(組成)에는 변화(變化)가 없었다. 그리고 굴비가공(加工)중 유리아미노산 총량(總量)은 원료(原料)에 비하여 3.6배(倍)로 증가(增加)하였다. 염장공정(鹽藏工程)중 유리아미노산이 원료(原料)에 비하여 3.5배(倍)로 증가(增加)하였으며, 염장후(鹽藏後) 건조공정(乾燥工程)중에는 원료(原料)에 비하여 양적으로 1%증가(增加)하여 거의 변화(變化)가없었다. 굴비제품(製品)에는 전(全)유리아미노산중 glutamic acid가 20.0%, lysine이 12.9%, leucine이 11.1%, alanine 이 10.1%, valine이 8.5%로서 이들 5종(種)의 아미노산이 전(全)유리아미노산의 62.6%를 차지하였다. 굴비의 유리아미노산중에는 좋은 맛을 가진 glutamic acid, 단맛을 가진 lysine, alanine 그리고 쓴맛을 가진 leucine등이 많이 함유되어 있으므로 이들 유리아미노산이 굴비의 독특(獨特)한 풍미(風味)에 중요(重要)한 구실을 할 것이라는 결론(結論)을 얻었다.

Gulbi, salted and dried yellow corvenia, is one of widely consuming fish foods in this country. This study was attempted to establish the basic data for evaluating the taste compounds of Gulbi. The free amino acids were analysed by amino acid autoanalyser. In the free amino acid composition of raw yellow corvenia, abundant amino acids were glutamic acid, alanine and lysine and then leucine, glycine, serine and threonine in order. Such amino acids like proline, valine, methionine, tyrosine and phenylalanine were poor in content. The changes of free amino acid composition in the extract of yellow corvenia during Gulbi processing was not observed. In the extract of Gulbi product, glutamic acid, lysine, leucine, alanine and valine were dominant holding 20.0%, 12.9%, 11.1%, 10.1% and 8.5% of total amino acids respectively. The amounts of total free amino acids of yellow corvenia increased more than 3.6 times as compared with that of raw sample during Gulbi processing. It is convinced that the characteristic flavor of Gulbi was attributed to such amino acids like lysine and alanine known as kweet compounds, as glutamic acid with meaty taste, and as leucine known as bitter taste.

3종(種)의 도토리 Quercus crispula Blume, Quercus serrata Thunberg 및 Quercus mongolica Fischer를 공시재료(供試材料)로 하여 그의 이화학적성질(理化學的性質), 호화도(糊化度), 점도(粘度) 및 X-ray회절등(回折等)을 실시(實施)한 결과(結果)는 다음과 같다. 1. 도토리는 지방(脂肪) 3.5~5.0%, 단백질(蛋白質) 7.2~7.7%, 당질(糖質) 71.6~76.3%, 회분(灰分) 1.9~2.2%였다. 2. Ether처리(處理)보다 85% methanol열처리(熱處理)가 보다더 순수(純粹)한 정제전분(精製澱粉)을 얻을 수 있었다. 3. 도토리 조제전분(粗製澱粉)은 일반(一般)으로 갈색(褐色)이나 정제전분(精製澱粉)은 회백색(灰白色)을 띄었다. 4. 호화(糊化)는 비교적 낮은 온도(溫度) 곧 61~68℃이고, 호화개시(糊化開始)는 Q.C.B.>Q.S.T>Q.M.F.의 순(順)으로 빨랐고 점도(粘度)는 Q.M.F>Q.S.T.>Q.C.B.의 차례로 높았다. 5. 도토리전분(澱粉)은 그 결정구조(結晶構造)가 감자전분(澱粉)과 옥수수전분(澱粉)의 중간성질(中間性質)을 띈 것으로 추정(推定)된다. 또 고온습열처리(高溫濕熱處理)로서 면간격(面間隔)이 긴쪽 부터 먼저 결정구조(結晶構造)가 파괴되었다.

The current experiment has been carried out in order to find the chemical and physical properties of three species of the acorn starch. The results of the experiment are as follows. 1. Acorn is made up of 3.5-5.0% of lipid, 7.2-7.7% of protein, 71.6-76.3% of carbohydrate, and 1.9-2.2% of ash. 2. The purifying method of ether treated is better than that of 85% methanol treated for isolating pure starch. 3. Initial gelatinization temperature of the acorn starches ranged from 61~68℃; Quercus crispula Blume was the lowest and Quercus mongolica Fischer was the highest. On viscosity, Quercus mongolica Fischer shows the highest and Quercus crispula Blume shows the lowest. 4. It is estimated that the acorn starch lie halfway between those of the potato starch and the corn starch. By drying at 100℃, the crystal structure is destroyed first in the farther part of the lattice distance.

보리가루-밀가루(중력분) 복합분에 있어서 보리가루 30%수준까지는 면대형성 및 절출등의 제면성이 양호하였고 겉보리가루보다는 쌀보리가루가 약간 우수한 제면성을 보였다. 이때 xanthan gum의 첨가는 이들복합분의 제면성을 향상시켰다. 그리고 쌀보리가루-탈지콩가루 복합분에서는 NSI(Nitrogen Solubility Index)가 높은 탈지콩가루만이 건면을 만들 수 있었으나 대체로 불량한 제면성을 보였다. 쌀보리가루-밀가루 복합분으로 만든 건면의 조리특성은 겉보리가루-밀가루의 그것보다 우수한 경향을 보였으며, xanthan gum의 첨가는 조리중 수분흡수 및 부피의 팽창을 촉진시켰다. 보리가루-밀가루 복합분으로 만든 건면의 조리후견고성 및 gumminess는 보리가루 혼합수준이 60%이상에서만이 밀가루 단독의 경우보다 저하되었으며, 쌀보리가루-탈지콩가루 복합분으로 만든 건면의 조리후 견고성은 특이하게 높은 현상을 보였다.

A barley (20-30%)-wheat (80-70%) flour gave generally acceptable noodle-making characteristics, in which naked barley flour showed somewhat better results than covered barley flour. And also for over 40% barley flour in the barley-wheat flour, the addition of xanthan gum improved the noodle-making characteristics. A naked barley-defatted soy flour could make dried noodles with only high NSI (Nitrogen Solubility Index) defatted soy flour, however this mixture was not considered to be feasible for noodle-making. In cooking characteristics of barley-wheat flour noodles, naked barley flour was more acceptable than covered barley flour and xanthan gum influenced the water absorption and volume expansion of noodles during cooking. The firmness, cohesiveness and gumminess in cooked noodles made of the barley-wheat flour increased and then decreased as the amounts of barley increased. The mixing of defatted soy flour (high NSI) to naked barley flour increased the firmness and gumminess of cooked noodles.

The conventional meju samples were collected from various districts and the improved kokja were prepared in laboratory. The changes of components in the process of the conventional soysauce and the improved soysauce preparation was studied. The experimental results are as follows; 1. The conventional soysauce is less than the improved soysauce in the extent of utility of total nitrogen. 2. The former is less than the latter in amino-type nitrogen content.

단백질 분해효소로서 넓은 특이성을 가지고 있는 Pronase를 사용하여 두 가지의 다른 방법으로 고정화효소(固定化酵素)를 제조하였다. Sepharose에 직접 결합시킨 효소는 원래 효소활성도의 22.6%을 유지하고 있었지만, Extension arm으로서 -CH₂-기의 수가 6, 10, 12를 가진 w-amino-alkyl group을 사용하여 Sepharose에 결합시킨 효소는 원래 효소활성도의 거의 100%를 다 유지하고 있었다. 제조한 고정화효소(固定化酵素)의 pH효과, 온도효과 및 Km을 조사하였으며 GRAS list에 들지 않은 Pronase를 식품가공에 이용하는 가능성을 고찰하였다.

Two kinds of sepharose-bound pronases were successfully prepared. The immobilized pronase, directly coupled to cyanogen-bromide activated sepharose, retains 22.6% of original specific activity against casein. However, w-aminoalkyl sepharose immobilized pronases, in which extension arms of w-aminoalkyl group (number of -CH₂- is 8, 10, and 12) are used, retain almost 100% of original specific activity. Studies of enzyme stability, pH dependence, temperature dependence, and Km values are presented.

한국산 쌀보리 2개품종 및 겉보리 2개품종을 Buhler test mill에 의하여 제분(제분수율은 쌀보리 약 70%, 겉보리 61%) 한 후 주요 무기질 및 비타민, 아미노산을 분석하여 다음과 같은 결과를 얻었다. 무기질성분중 P의 함량(0.19-0.27%)이 높았고 K 는 0.18-0.27%, 그리고 미량의 Ca 및 Fe가 함유되고 있었다. 비타민중 A 및 C는 거의 함유되어 있지 않았고 B₁, B ₂및 niacin은 각각 3.6-5.7, 0.9-1.6 및 27-39㎍/g의 범위를 보였다. 보리가루류의 아미노산조성은 품종간에 다소 차이를 보였으며 제한아미노산은 lysine으로서 보리가루 단백질의 amino acid score(FAO 아미노산 표준구성 기준)는 부흥이 61.7, 기타는 모두 58 내외를 보이고 있었다.

According to the intercombined review of chemical and biological investigation it has been noted that the metabolizable energy per gram dietary protein of mixed diet of daily intake patterned by Korean population has been found 3.4-3.6 Cal., which entails 10-12% level of the protein calorie percentage of total metabolizable energy, the biological value being fallen within the scope 63-73. The structure of dietary protein has revealed that the lysine and isoleucine were primary limiting amino acids and threonine secondary limiting as a general trend, however, it is assumed that the ultimate nutritional effect of dietary protein might be restricted uniformly among regions by the amount of lysine, since the lysine availability has been yielded as low as 72-82% level. As for the net protein utillization NPUst falls in the range of 52-62 and the NPUop 47-58. In either part the mountainous region has demonstrated lowest value and the urban area highest, these trend being obviously associated with the ratio of animal protein relative to the vegetable origin. The net dietary protein calorie percentage (NDpCal %) has been found within the range of 5-7 that may be capable of meeting the requirement for the maintenance of adult, though for the growth it is insufficient. Present level of total caloric intake would not influence on the fate of protein value of prevailing regional diet in terms of caloric restriction, since the present intake of food energy is higher than the lower limit of caloric intake that would impair the biological performance of dietary protein fed ad libitum basis. Based on the protein efficiency, the adequacy of current level of protein intake was analyzed in terms of utilizable protein, and it has been demonstrated that the 37.8g of utilizable protein in the fishery region and 38.2g in the mountainous region were bellow the FAO recommendation. Accordin to the hematological study it may be interpreted that the anemic symptoms of the mountainous region has some possibility of being related to the inferior status of dietary protein in quality as well as in quantity.