Earticle

In order to confirm the presence of putative glucoamylase gene in Tricholoma matsutake genome, the genomic DNA was prepared from T. matsutake NBRC30773 strain and was used as template to clone the glucoamylases gene (TmGlu1). We obtained the nucleotide sequence of TmGlu1 and its franking region. The coding region (from ATG to stop codon) is 2,186 bp. The locations of exons and introns were determined from the nucleotide sequences of 3’- and 5’-RACE PCR and RT- PCR products. On the other hand, to investigate the relationship between composition of medium and glucoamylase expression, we checked the expression level of glucoamylase gene by realtime reverse transcription PCR and measurement of glucoamylase enzyme activity. It was found that enzyme activity of glucoamylase was very low in different medium. Expression of glucoamylases gene appeared to not be affected by different carbon source.

To develop a new cultivar of King oyster mushroom(Pleurotus eryngii), G09-21 as parental strain was selected by the method of Di-mon crossing between monokaryotic strains derived from ASI 2824(Keunneutari No.2) and dikaryotic strain ASI 2887(Aeryni 3). The Pe21-51(G09-21-10 x 2844-11) was shown the best cultural characteristics, selected to be a new cultivar and named as ‘Song-A’. The ‘Song-A’ was formed incompatibility line distinctly in the confrontation growth of parental strains Keunneutari No.2, Aeryni 3 and ASI 2844. The optimum temperature for mycelial growth, fruiting body development and pH arrange were 25~30℃, 14~16℃ and pH5~8, respectively. Fruiting body production per bottle was about 94.7±29.5 g which is almost 106% quantity compared to that of other cultuvar Keunneutari No.2. And also the stip is thick and long but the number of available stipe is few. Analysis of the genetic characteristics of the new cultivar ‘Song-A’ showed a different DNA profile as that of the control strains, Keunneutari No.2, Aeryni 3 and ASI 2844, when RAPD(Random Amplified Polymorphic DNA) primers URP4 and 7 were used. This new cultivar ‘Song-A’ of Pleurotus eryngii is characterized by a small number of primordia formation and the stip is thick and long. Therefore, we expect that this new strain will save of labor and cost by without culling work.

Artificial cultivation of Lyopyllun decastes is costly because the high expense is required for soil covering and bark. Therefore, most farmers of Lyopyllun decastes have recognized it is difficult to cultivate it. The purpose of this study is to reduce the cost of cultivation of Lyopyllun decastes by applying new cultivation method and introducing mutation of fungi. In this study, a new method of practical artificial cultivation was developed through many experiments using fermented pine sawdust and wheat bran. In conclusion, the method of practical artificial cultivation of Lyopyllun decaste is simple and cost efficient because neither soil covering nor bark is required.

We have researched on methods which can reduce fruit-body malformations of king oyster mushroom (Pleurotus eryngii). We collected many pathogens from diseased fruit-body or malformated fruit-body and identified with chemicobiological test and microscope. The factors of fruit-body malformations and increment of contamination during the pin- heading induction time were researched with ventilation amounts in growing room. When the pathogens having high pathogenicity were inoculated in spawn running bottle and at pin-heading induction time, symptom appeared or not appeared in according to air ventilation amounts in growing room. During the pin-heading induction time, humidity degree in growing room have kept of high level and air ventilation amounts were so little that fruit-body malformations ratio was high. But, even though pathogens were inoculated at the surface of bottle factitiously, if air ventilation amounts were enough, fruit-body malformation ratio was low.

This study was carried out to develop a method related to measuring the temperature of sterile medium in bottle cultivation. When the medium is sterilized, the device is able to be inserted inside of the medium and the temperature can be directly measured in real time although high temperature and pressure are detected in the sterilization. This device can be able to measure the sterilization temperature in short intervals inside of autoclave and medium used in bottle cultivation. As the method were applied to the field cultivation of mushroom, we could produce mushroom in consistent manner through the optimum sterilization of the medium.

Sparassis crispa(Cauliflower mushroom) was an edible mushroom that shows remarkably high contents of ß-glucan compared to other edible mushroom. In this study, we aimed to select suitable conditions and materials for mycelial growth and fruit body production. The longer saccharification time of barely result in higher sugar content for eight hours. The optimal sugar content of media for mycelial growth was showed at level of 6 °Brix. Optimum temperature and pH for mycelial growth in liquid spawn were 25℃ and pH 5.0~6.0, respectively. In case of sawdust was used Larix kaempferi as main material, the fruit body yield and cultivation period of supplemented with 10% wheat flour and 20% corn flour were highest and fastest, respectively.

This study was carried out to investigate anti-inflammatory effects of collected mushroom in Korea. Anti- inflammatory effects analysis was followed by MTT test and nitric oxide inhibition activity. Thirty mushrooms were screened and twelve mushrooms were selected about MTT assay and three mushrooms were good at nitric oxide inhibition test. In our result, three mushrooms: Amanita virgineoides, Tylopilus neofelleus, Armillaria tabescens were good resource for anti-inflammatory effects and to be followed more research about relat.

This study was performed to investigate the antibacterial activities and the minimal inhibitory concentration (MIC) of 6 strains of Beauveria bassiana against mulberry pathogenic bacteria. The antibacterial activities and the MIC were measured using paper disc method and broth dilution method, respectively. The antibacterial activities were found out just B. bassiana J200, and shown at 13 mm from Erwinia rhapontici KACC 10407 and at 17 mm from Pseudomonas syringae KACC 10390 and Xanthomonas campestris KACC 12134. The MIC were all observed at 4.0% from E. rhapontici KACC 10407, P. syringae KACC 10390 and X. campestris KACC 12134. The results suggest that B. bassiana could play a good role for biological control against mulberry pathogenic bacteria.