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In order to reduce the loss in the circulation of agricultural products, and to reduce the total cost, this article minimizes the operation cost by establishing an objective function when designing the cold chain logistics distribution network. Considering the characteristics of the cold chain products, this paper regards construction cost of network nodes, transportation cost and damage cost as the main factors in establishing an objective function. As the cold chain logistics is correlated to the demand of service time, this article comes up with a concept of "service radius", which transformed the transportation time between logistics nodes into the service radius of logistic nodes. The model is verified through a series of constraint conditions to express the relationship between the various decision variables. An example is given in the end, which demonstrates the application of this model in a vegetable firm distribution logistics network planning. The final computation results illustrated that the model is effective.

The newly discovered Th2 pro-inflammatory cytokine, interleukin-17E belongs to the member of IL-17 family. In this study, bioactive recombinant mutated human IL–17E (rhIL–25) was synthesized using overlapping PCR strategy and amino acid mutations were carried out using site directed mutagenesis. Four cysteins at 78th, 83rd, 136th and 138th positions were involved in disulphide bond formation and were responsible for biological activity of mature protein. These four cysteins were replaced with serine using nucleotide substitution and the desired outcome was cloned into expression vector pRSET-A followed by expressed in a salt inducible Escherichia coli GJ1158. The transformants were selected by ampicillin resistance marker and also by DNA sequencing. SDS–PAGE analysis confirms 17.06 kDa purified protein against low molecular weight protein marker. Protein quantification was carried out using Lowry’s method. Approximately 104 mg/L of recombinant IL-17E was produced at 37 ℃. Biological activity of protein was determined by the release of IL–6 from PBMC cells using rhIL–17E. This is the first report on production of interleukin-17E structural variant with enhanced specific activity without compromising the biological activity.

This research aims at the designing and implementation of an Automatic Voltage Regulator (AVR) with higher precision and hysteresis. AC power supplied by PDB (Power development board) in Bangladesh is subjected to variation from time to time. Moreover in rural areas supplied voltage remain lower than specified most of the times. This poses a considerable threat to the sophisticated electronic devices like computer, refrigerator, television etc. So ensuring the input voltage to remain in a tolerable pre-specified limit has become a necessity in rural as well as some urban areas. Current systems available locally lacks precision and suffers the problem of oscillating between two output voltage and hence creating surge at the output which can damage valuable electronics. This research handled both shortcomings and introduced in the tolerable range of 215-237 volt using several taps. Hysteresis has been introduced while changing from one level to other and thus preventing oscillation.

Profitability is the main goal of every software organization. Software companies develop products to capture greater market place and gain the attention of targeted customers or stakeholder. In the past the software engineering field was not able to determine the stakeholder’s value of the developing product as mostly systems were based on value neutral setting. With the emergence of Value based software engineering (VBSE) the traditional software engineering discipline is vastly changed as during product development the stakeholder’s value proposition plays an important part and the development is done accordingly. In this article I cover some important aspects of value based software engineering regarding their impact in software reusability and quality. Different value based pricing criteria are discussed. Empirical research and value based selection of best suitable automated tools and existing Component off the shelf (COTS) and conflicts between different stakeholders of the same system are also taken into account. In the 3rd section Critical review of the all papers is performed and their plus and negative points are mentioned. At last the Conclusion and future work in value based software engineering field is presented.

In this paper, a new approach is proposed to investigate the characteristics behavior of concrete under uniaxial and biaxial compression using the theory of plasticity. This approach is based on artificial neural networks (ANNs), especially radial basis function (RBF) in conjunction with the models of theory of plasticity. The main advantage of the proposed approach is to estimate the quality of the results with accuracy equivalent to the experiments. Another advantage of the proposed ANNs models are that it takes into account the uniaxial as well as the biaxial compression strain. The proposed models were evaluated against several experimental results available in the open literature for the behavior of the force and deformation of the two types of compression tests. Good agreement has been found between our models and those presented elsewhere.

It has been well established that advanced surface coatings on cutting tools improve wear resistance by modifying the contact conditions between the chip and tool interface. As a result of the recent developments in cutting tool industry, coated tools have made a significant contribution to the metal cutting operations in terms of tool life, cutting time and machining quality. The challenge of modern machining industries is focused mainly on the achievement of high quality, in terms of work piece dimensional accuracy, surface finish, high production rate, less wear on the cutting tools, economy of machining in terms of cost saving and increase the performance of the product. In general, the most important point in machining processes is the productivity, achieved by cutting the highest amount of material in the shortest period of time using tools with the longest life time. The present research work describes the development, Mechanical, Tribological performance of Nano material coating of TiN,TIC, on Tungsten Carbide cutting tool. The Mechanical, Tribological properties of TiN,TIC, are to be compared with uncoated Tungsten carbide cutting tool. And also different coating methods like Chemical Vapour Deposition, Physical Vapour Deposition Method, can be used for comparision.The present work will help to find the tool life and wear behaviour of the each coated tool and it will help to find the best tool coating applicable for the cutting tool. The experiments of Mechanical, Tribological properties tests have to be conducted as per ASTM standards. SEM analysis has to be done for investigating the surface morphology of Tungsten Cutting tool. The coated cutting tool have to be modelled using suitable assumption and analyzed by means of finite element method using ANSYS software. Both results of Experimental and ANSYS software are to be compared.