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하수처리장의 활생오니를 분리원으로 하여 수십종의 PHA 생산균을 분리하였다. 일반적으로 hy­d droxyvalerate monomer unit의 전구물질로 알려져 있지않은 glucose로부터 비교적 많은 PHA를 생산 하는 균주를 공시균으로 선정하여 형태학척, 배양적, 생리학적 제 특성을 검토한 결과 Pseudomonas 속으 로 통정되었다. 균체 생육을 위한 최적 배양온도 벚 배양 pH는 각각 와 7.0이었으며, 최적 탄소원 으로셔 glucose 1 %, 최적 질소원으로서 (NH4)2SO4 0.2%, K1HPO. 0.3%, KH2PO4 0.45% 였다. 최적 P PHA 생산조건을 조사하기 위하여 2단계 배양법을 이용하였다 PHA 생산은 배지성분중 NH4,O4, Mg가 결핍되 였을 때 향상되었고, 그중 NH4, 의 결핍시 PHA 축적률과 HV monomer의 함량이 가장 높았다. C/N molar ratio 95.2에서 PHA 축적률이 가장 높았다. 공시균주 Pseudomonas sp. HJ는 alkane, alkanoic acid, alcohol을 탄소원으로 하여 P PHB/HV를 생산하였다. PHA의 생산량과 HV monomer의 함량은 이용된 기질에 따라 다양하였으며, 특히 hexadecane와 propiOnate를 탄소원으로 하였을 때 PHA중의 HV monomer의 함량이 49~74mol %로 매우 높았다. IH-NMR로셔 공시균으로 부터 분리정제된 PHA의 조성을 분석한 결과 PHB/ H HV copolymer임을 알 수 였였다

To produce PHA(polyhydroxyalkanoic acid) from microbr, dozens of microorganism have been screened from sewage sludge. Selected a strain HJ out of 50 strains of PHA producing bacteria has a capability of accumulating large amounts of PHB/HV copolymer when grown in batch culture with a single carbon source (glucose) that was not generally considered as precursor of hydroxyvalerate monomer unit. The strain HJ was identified as the genus Pseudomonas with respect to morphological, cultural, and biochemical characteristics. The optimal temperature and pH for cell growth were 37℃ and 7.0. The optimal medium compositions for cell growth were glucose 1% as a carbon source, (NH4) 2SO4 0.2% as a nitrogen source, K2HPO4 0.3%, and KH2PO4 0.45%. TO investigate she optimal condition for PHA production two-step cultivation method was employed. PHA production was inducted by deficiency of NH4+, SO4-2, Mg+2. Besides carbon source, deficiency of all nutrients stimulated PHA productivity but deficiency of NH4+ stimulated the most HV monomer content. The highest PHA production was C/N molar ratio 95.2. Pseudomonas sp. HJ was also able to pyoduc PHB/HV copolymer when cultivated on alkane, alkanoate, alcohol as carbon sources. The contents of PHA and she proportions of hydroxyvalerate monomer units varied depending on the carbon sources. Especially Pseudomonas sp. HJ was able to incorporate hydroxyvalerate into PHB/HV to level as high as from 49 to 74 mol% when grown in a medium containing hexadecane and propionate. The purified PHA was identified PHB/HV copolymer by HNMR analysis.

A mathematical model using local thermodynamic equilibrium isotherms for adsorption in encapsulated adsorbent is proposed in order to optimize the design parameters in situ bioproduct separation process. The model accurately follows the experimental data on the adsorption of berberine, secondary metabolite produced in Thaictrum rugosum plant cell culture. The adsorption rate on encapsulated adsorbent is compared with that on alginate-entrapped adsorbent. The result shows that the higher loading capacity in encapsulated adsorbent is mainly due to the increase in the maximum solid phase concentration. Based on the adsorption rate and loading capacity, the encapsulated adsorbent would be more useful than the entrapped adsorbent when used in situ bioproduct separation process. Design parameters in situ bioproduct separation process, such as the size of the capsule, membrane thickness, the ratio of capsule volume to bulk volume, the ratio of single capsule volume to total capsule volume and the adsorbent content in the capsule, are evaluated by using the model. The ratio of single capsule volume to total capsule volume is the most effective parameter for adsorption of berberine on encapsulated adsorbent.

S.cerevisiae에 의한 에딴올 발효에셔 생성되는 부산물 인 acetic acid, acetaldehyde, glycerol, lactic acid, formic acid가 세포성장과 에탄올 생성에 미치는 영향을 고찰하였다. Acetic acid와 acetaldehyde 를 발효액 내에 투입하였을 때, 세포생장은 저해되 었으나, 에탄올 생성은 증가되었다. 한편, glycerol 과 lactic acid는 세포성장과 에탄올 생산에 거의 영 향이 없었다. Acetic acid와 acetaldehyde는 비성장 속도를 줄임과 동시에 정체기를 늘염으로써 세포성장을 저해하였다. 에단올 수율은 첨가된 acetic acid 와 acetaldehyde 농도에 비례하여 증가하였고, acetic acid 3g/l, acetaldehyde 2g/l 일 때, 최대가 되었다.

The major by-products in ethanol fermentation by Saccharomyces cerevisiae were glycerol, acetaldehyde, acetic acid, lactic acid, and formic acid. The effects of these by-products on the cell growth and ethanol production were studied. By adding acetaldehyde or acetic acid in the fermentation broth, the cell growth decreased while the ethanol production increased. But glycerol and lactic acid had nearly no effects on the cell growth and the ethanol production. Acetic acid and acetaldehyde inhibited the cell growth by diminishing the growth rate as well as by prolonging the lag phase. The ethanol yield increased with the elevation of concentrations of acetic acid and acetaldehyde in the fermentation broth. The maximum ethanol yield was obtained for 3g/l acetic acid and 2g/l acetaldehyde, respectively.

Gelatin 분해성 proteolytic enzyme 생성균인 B. subtilis 80021가 생산하는 세포외 단백칠 분해효소 생산을 위한 영양 요구성과 배양조건을 검토한 결과 는 다음과 같다. 영양요구성은 1.5%(w/v) glucose와 2.5% (w/v) yeast extract에서 효소활성도가 최고치를 나타내였고 metalion은 Co2+,Zn2+효소생성의 저해제로 작용한 반면 Fe2+[?][?][?]Mn2+에서는 촉진제로 작용 하여 최고 3.5배 효소생산생이 증가하였다. 아미노산 첨가 영향에서는 대부분의 아미노산이 효소활생 을 촉진시키는 것으로 확인되였다. 배지의 최적초기 pH는 7.0 부근이며 배양온도는 에서 효소에 따 라 다소 차이는 였었으나 배양시간 30시간 정도에서 최고의 효소생산성을 나다내었고 30°C에서는 42시간째에 효소활성도가 최고치를 나타내였다. Tannic acid 첨가에 대한 만백질 분해효소 생산에서는 발효 애지에 tannic acid 첨가시 완전한 효소생산 저해제로 작용하였으며 salicin을 탄소원으로 한 salicinase assay에서는 온도 빛 배양시간에 관계없이 효소활성 을 보이지 않았고 복합탄소원 이용에 대한 amylase assay에서는35℃ , 36시간째에 최대의 효소활성을 나타내였고 Invertase assay에서는35℃ , 30시간째 에 최대 효소활성도 2.5unit/ml을 나타내였다. 또한 gelatin 가수분해능에서는 45% (w/v)의 gelatin 용 액의 점도가 발효조효소액에 의해 96% (mPas)의 정도저하를 가져오는 것으로 확인되었다.

Nutritional requirements and cultural conditions for the production of extracellular gelatin-degrading proteolytic enzyme by Bacillus subtilis B0021 were investigated. Optimum carbon source for proteolytic enzyme production was salicin, but it was substituted by glucose for economical reason. The fermentation medium giving a maximum proteolytic enzyme activity was consisted of 1.5%(w/v) glucose, 2.5%(w/v) yeast extract, and 0.001%(w/v) manganese sulfate and 0.002%(w/v) ferrous sulfate. Proteolytic enzyme activity of B. subtilis B0021 was completely inhibited by 0.5%(w/v) tannic acid. Initial pH was optimal at 7.0 and the enzyme activity in the flask culture usually reached a maximal level after 36 hours of fermentation at 30℃. In the 5l fermentor fermentation at 30℃, enzyme activity was maximum at 36 hour of cultivation but after this enzyme activity was decreased rapidly. Initial viscosity of 45%(w/v) gelatin(2,800mPas) was decreased rapidly to 96%(mPas) after hydrolysis for 4hr at 40℃ by crude enzyme of B. subtilis B0021.

The high viscous r-polyglutamic acid(r-PGA) from alkalophilic Alcaligenes sp. was purified and its solution property was investigated. The intrinsic viscosities for Na+ form and H+ form of r-PGA were 31.1 and 0.38dl/g, respectively. The viscosity of H+-PGA was not influenced by pH or salts while that of Na+-PGA was influenced. The intrinsic viscosity of Na+-PGA solution decreased remarkably at the alkaline or acidic pH and showed the sharp decrease when salts were added. r-PGA exhibited the property of the polyelectrolyte showing the .sharp decrease of intrinsic viscosity by the addition of NaCl, and intrinsic viscosity of dilute solution with the low concentration of NaCl was exponentially dependent on temperature and its temperature dependency increased with increasing NaCl concentration. The chain stiffness, coil overlap parameter and critical concentration of Na+-PGA were 0.08, 5.25 and 0.07g/dl, respectively.

전통도료인 황칠의 재현을 위한 황칠나무의 다량 증식 및 이용에 관한 연구의 일환으로 황칠나무의 자생지 분포조사 및 황칠의 성분 분석을 실시하여 다음과 같은 결론을 얻였다. 1. 황칠나무의 자생지는 완도, 상황봉 등 8개 지역이었으며, 해남 두륜산은 처음으로 자생지로 확인되었다. 2. 황칠나무는 해발 lOO~450m 사이에셔 자생하고 있으며 해발 200m 부근에서 빈도가 가장 높게 냐다났다. 또한 자생지의 토양은 pH 4.9~5.8로 약 산성이며, 수분 함유량은 16.5~27.4%로 비교적 습 기가 많은 지역에서도 자생하는 것으로 나타났다. 3. 군락지 조새에서 보길도와 제주도가 우점도 및 군도가 교목층에셔 3.3으로 높게 나타났으며 완도는 아교목층에서 많이 나타났다. 4. 생육상황은 보길도에서 가장 높게 나타나 흉고 직경 1.O~20cm, 높이 O.2~9m 까지의 개체가 다수 확인되었다. 5. 황칠의 성분을 분석한 결과 대표적 인 성분이 B- selinene과 capnellane-8-one을 확인하였다.

As a part of the .study on the multiple propagation and use of Dendropanax mcrbifera, distribution of this tree and component analysis of Golden Lacquer were investigated to reappear the Golden Lacquer, traditional paints. These results were summarized as follows. The natural growth habitat of Dendropanax morbifera was observed in 8 sites(Wando, Sanghwangbong, etc.), we could be found that Duryun nountain in Haenam for the first time. Dendropunax morbifera has grown from 100 to 450m above the sea level, appeared high frequency in the 200m. The soil pH of natural growth site was pH 4.9 to pH 5.8. Dendropanax morbifera grew in the area containing considerable moisture in that its contents was 16.5% to 27.4%. In community observation, dominance and community index of Bogildo and Chejudo was 3.3 in the forest tree layer and Wando was showed in the sub-forest tree layer mainly. In the growth conditions, Bogildo appeared the highest growth state that diameter of height of human chest was 1.0 to 20cm and tree height was 0.2 to 9m. As result of IR and GC-MS analysis, main component of Golden Lacquer was B-selinene and capnellane-8-one

S. platensis의 옥외연속 대량배양을 위해 125P 배양조를 설계, 제작하여 배양조건에 따른 생육 변수를 검토하였다. 0.30(1/day)의 비생육 속도와 1.69(g/l) 의 최대 세포농도가 회분배양에셔 얻어졌 으며 유가삭 배양에셔 비생육 속도와 최대 세포농도 는 각각 0.22(1/day)와 1.75(g/l) 였다. 연속 배양 에서 얻어진 상.하층 배양조의 최대 균처l생산생은 각각 0.44와 0.43(g/l/day) 였다. 따라서 인위적으로 온도, 광도 등의 생육조건을 고려하여 S. platensis를 연속 배양할 경우 경제성이 클 것으로 사려된다. 아울라 top-driven stirrer와 air sparging 장치를 이용하여 교반 효과를 비교한 결과 top­d driven stirrer를 이용한 배양조의 균채 생산성이 다소 높은 갯으로 냐타났다.

An open pond type photobioreactor for mass cultivation of S. platensis was designed and the growth parameters from different cultivation processes were compared. 0.30(1/day) of specific growth rate and 1.69(g/l) of maximum cell density were obtained from batch cultivation. In fed-batch cultivation, specific growth rate and maximum cell density were estimated as 0.22(1/day) and 1.75(g/l), respectively. Maximum biomass productively from continuous cultivation was obtained as 0.44 (g/l/day). It proves that an outdoors-mass cultivation of S. platensis considering optimal environmental condition is economically feasible. In addition, the biomass productivity was studied in two different mixing systems such as agitation and air sparging methods. The biomass productivity by an agitation method was better than that in an air sparging method.

Polyhydroxyalkanoates[PHAs] are the polyester of hydroxyalkanoates(HAs) synthesized by numerous bacteria as an intracellular carbon and energy storage compound and accumulated as granules in the cytoplasm of the cells under unbalanced growth condition in the presence of excess carbon source. Even though PHAs have been recognized as good candidates for biodegradable plastics, their high price compared with conventional plastics has limited their use in a wide mange of applications. To reduce the high production cost of PHAs, many group of scientists have devoted much efforts to improve productivity by employing various microorganisms and by developing efficient culture techniques. The strategies of producing PHAs to a high concentration with high productivity and their potential applications are reviewed.

상하수도 및 폐수처리에 많이 사용되고 있는 무기 및 유기합성 고분자응집제를 미생물 응집제로 대체 하기 위한 연구의 일환으로 토양으로부터 목적 미생물을 분리하여 형태학적, 생리학적 성질을 조사한 결과 Aspergillus sp. JS-42로 명 명 되 었다. 또한 Aspergillus sp. JS-42의 응집제 생산을 위한 최적배지 벚 최적 배양조건은 다음과 같이 설정하였다. 배지 조성은 3.0% glucose, 0.2% yeast ex ttract, 0.1 % (NH4)2SO4,0.05%CaCI2.2H2O 의 경우가 응집제 생산에 우수하였으며, lJ~양온도 및 초 기 pH는각각 25℃와 pH 7.0의 경우가최적이였다. 이 배양조건 하에서 90시간 전후로 배양하였을 때 응집제 생산이 최대가 되었으며 이때는 screenmg시 의 기초배지에 비해 응집활성이 거의 8배 정도로 향 상되었다. 이 물질은 각종 기질에 대해 넓은 작용범 위와 강력한 응집능을 발휘하였으며 pH 및 열처리 에 대해 극히 안정한 물질이였다. 또한 이 물질은 점성이 강한 고분자성인 다당류의 일종인 것으로 판단 되였다.

A fungal strain, designated Aspevgillus sp. JS-42, was isolated and shown to produce an extracellular polysaccharide used as a bioflocsulant. The optimal medium composition for the production of flocculant with Aspergillus sp. JS-42 was glucose 3.0%, yeast extract 0.2%, (NH4)2SO40.1%,CaCI2,2H20.05% in distilled water. The optimum culture temperature and optimum culture pH for the production of the flocculant were 25℃ and pH 7.0, respectively. The highest production of flocculant was observed after 90 hours of cultivation at the optimal condition. The flocculant could efficiently flocculaled the tested solids suspended in aqueous solution and was stable at high temperature(100℃) and to pH range of from 2 to 10. The flocculant seems to be a kind of high viscous polysaccharide.

Target-sensitive(TG-S) liposomes, which have the antibodies coupled on the surface of liposome and can release their entrapped contents by the binding of antibodies with the specigic target cells, were prepared and employed to study the release of calcein and the selective delivery of an anticancer agent, doxorubicin(DOX). The monoclonal antibody, Y3, used for the preparation of the TG-S liposome was one against major histocompatibility complex class 1 of mouse(MHCI, H-2Kｂtype) and the target cells were EL-4 and RMA, which have the MHC1, H-2Kbtype on their membrane surfacem. The release of calcein from TG-S liposome occurred when the target cells were contacted with liposomes and it was proportionally increased with the rise of binding capacity of antibody coupled on the surface of liposome to the target cells. The experimental results of drug delivery were similar to the cases of calcein release. The viability of specific target cell, EL-4 with liposomal DOX was not so different from that with the free DOX, while for the non-specific target cell, Yacl(H-2Kf), the cell viability with Iiposomal DOX was much higher than that with free DOX. This shows the fact that the liposomal DOX can be efficiently delivered to the specific target cells, while it was not the case for the non-specific target cells. And the drug delivery was lnhibited when the free antibody of Y3 was added in the contact process between EL-4 and TG-S liposomes, which means the drug delivery occurred mainly by the destabilization of TG-S liposomes. From these results, we could conclude that the selective drug delivery to specific target cell using the TG-S liposome would be feasible.

Polyamine이 Agrobacterium rhizogenes에 의해 유도된 시호(Buplerum falcatum L.)의 모상근 배양 에 미치는 영향을 알아보고자 하였다. Polyamine을 처리하여 현탁배양했을 때 대조구에 비해 생체중량 과 건조중량을 모두 증가시 켰으며, 특히 spermme (10uM,100uM) 처리구에셔는 약 7배 이상 증가시 켜 시호모상큰 배양에 가장 효과적이었다. 또한 polyamine을 처리하여 시호모상근을 현탁배양한 후 내재 polyamine을 측정한 결과 putrescine(10uM,100uM) 처리구와 spermine (10uM) 처리구에서 대 조구보다 putrescine, spermidine, spermine의 함량 이 상대적으로 높게 나타났다. Polyamine에 의한 B-glucan synthetase II 의 활성은 대조구에 비해 증 가시켰으며, 특히 spermidine(100M)과 sperm me () 처리구에서는 각각 180%, 220% 정도의 효소활성을 촉진시켰다. 이러한 결과는 polyamine이 생장조절물질로서 시호모상근 배양에 중요한 인자로 작용할 수 있음을 시사해 주었다.

During culture the effect of polyamine on hairy root of Buplerum falcatum by infection of Agrobacterium rhizogenes was studied. The fresh and dry weight of hairy root which cultured for 3months in MS medium increased about 7-fold when spermine at 10uM, 100uM was treated. After suspension culture of B. falcatum in MS medium containing putrescine(10uM, 100uM) or spermine(10uM), the contents of endogenous polyamine (putresclne, spermidine, spermine) was higher than that of control. The B-glucan synthetase II activity by polyamine treatment was increased： especially spermidine (100uM) and spermine(100uM) stimulated it by about 180% and 220% respectively. These results suggest a possible role of polyamine as growth regulator in B. falcatum hairy root cultures.

토양으로부터 분리 동정된 균주인 Bacillus polymyxa KS-1을 이용하여 이들이 생산하는 미생물 생 다당듀 KS-1의 생산을 위한 배지 및 배양조건을 조사하였다. 다당류 KS-1의 생산을 위한 애지의 조성은 증류수 1 liter당 glucose 30g, yeast extract 2.5g,KH2PO4w.5g,NaCI0.5g,MgSO4,7H2O0.3g,CaCO30.05g,FeSO4,7H2O0.05g,[?][?][?][?][?][?][?][?][?]MnSO4,4H2O0.05g 이 첨가된 배지에서 높은 정성 벚 다탕류 생산 을 얻을 수 있었으며, 이때 배양조건은 초기 pH와 배양온도는 각각 7.0과 , C에서 다당류 생산이 가 장 좋은 갯으로 조사되였다. 또한 이상의 최적조건 에서 발효조를 이용한 경우 통기량 빛 교반속도를 각각 2vvm과 400rpm으로 조절하여 배양한 경우 생산량은 liter당 약 10.3g의 다당류 KS-1이 얻어졌다.

Optimized fermentation medium and cultural conditions for the production or exopolysaccharide KS-1 with Bacillus polymyxa KS-1 was following as; 30g g1ucose, 2.59g yeast extract, $2.5g KH_2PO_4, 0.5g NaCl, 0.3g MgSO_4.7H_20, 0.1g CaC0_3 0.05g, FeSO_4.7H_2O, and 0.05g MnS0_4 . 4H_20in 1 liter distilled water. The exopolysaccharide production was influenced by the by the temperature and pH, the optimal conditions for the production of exopolysaccharide KS-1 seemed to be 30℃ and pH 7.0, respectively. About 10.3g/l of maximum exopolysaccharide was obtained al the initial pH 7.0, 30℃, 2vvm of aeration rate and 400 rpm of impeller speed in a jar fermentor.

대두유 및 미강유의 찌꺼기유내에 존재하는 고부가가치의 토코페롤을 농축하기 위하여, 찌꺼기유중의 불포화 유라지방산의 제거에 에타서l시스반응을 적용하고 그에 따른 농축효과에 관하여 연구하였다. 함유된 스테롤은 흔합용매법에 의해 제거하였고, 메타세시스반응은 헬륨분위기의 회분삭반응기를 사용하였다. 대두유 및 미강유의 찌꺼기유에 함유되어 있는 토코페롤은 헥산용매중에서 또는 공기존재하에서 가열하면 쉽게 소실되었다. 스테롤을 제거한 여액의 에타세시스반응에 있어서, 사용한 Re2O7.Al@O3 촉매는 12.8% (w/w)의 담지율이 가장 좋았는데, 이는 고담지율에서의 [Re2O7]과 테트라에틸주석이 반응하여 메타세시스에 유효한 활생종을 생성하기 때문으로 생각되었다. 촉매량은 원액 109당 O.5g의 경우가 최적이었으며, 반응온도는 25℃가 가장 좋았다. 이 사살들로부터 찌꺼기유중의 토코페롤을 새로운 메타세시스반응에 의해 고농도로 항축할 수 있음을 알 수 있었다.

The effects of metathesis for concentrating the tocopherols from soybean and rice-bran scum oils were studied by using the batch reactor under helium atmosphere. The contents of tocopherols in the scum oils decreased consticuously when heated under air atmosphere or when kept in hexane solution above 5 days even at room temperature. The sterols in the scum oils were removed by the mixed solvent method. Metathesis of the sterol-removed scum oils in hexane was performed over Re2O7/Al2O3 and WO3/Al2O3 catalysts, and the concentrate was obtained by distillation in vacuum at 190℃. The effect of metathesis was evaluated as relative ratio of -tocopherol in the concentrate to that in scum oil. The maximum ratio for both scum oils was obtained on 12.8%(w/w) Re2O7/Al2O3 catalyst which formed effectively the active sites for metathesis by the reaction between the added tetramethyltin and Re2O7 on the surface of the catalyst. The optimum amount of the catalyst was 0.5g pre l0g scum oil, and the optimum reaction temperature was 25℃ for both scum oils. The metathesis was more effective in rice-bran scum oil than in soybean scum oil. These facts indicated that the tocopherols in the scum oils can be highly concentrated by applying metathesis.

재조합 대장균의 유가식 발효에서 초산 생성 억제를 위해 세포 비성장속도를 제어하였다. 세포 비성장속도가 알정한 값으로 제어되는 유가삭 발효를 수 행하여 세포 비성장속도에 따른 세포생장, 포도당 소모량, 초산 생성량 및 재조합 단백질인 H lactamase의 발현 등을 관찰하였다. 세포 비성장속 도의 제어값이 클수록, 포도당이 축척되고 초산 생 성이 늘어나며 재조합 단백질의 서l포당 발현율은 떨 졌다. 그러냐 높은 세포 비성장속도의 경우에도 m methionine을 첨가하여 초산의 저해작용을 경감시킴 으로써 단위세포당 발현율을 높은 값으로 회복시킬 수 있었다

Specific growth rate was controlled for the repression of acetic acid formation in the fed-batch fermentation of recombinant Escherichia coli. With controlled specific growth rate, we studied the effect of the specific growth rate on cell growth, glucose consumption, acetic acid formation, and the expression of recombinant protein (B-lactamase). High specific growth rate caused the accumulation of glucose and acetic acid, and lowered the production of recombinant protein. However, the addition of methionine recovered the gene expression by alleviating the negative effect of acetic acid at high specific growth rate.

본 연구에서는 약물 전달체(키토산, 키토산.엽, 술폰화키토산)에 prednisolone을 분산시켜 제조한 고분자 매트릭스를 poly (DL-lactide)로 피막을 형성시킨 후 pH 1.2와 pH 7.4 인산염 완충용액에서 약물 방출실험을 하였다. 약물 방출시간은 pH 1.2에서 보다 pH 7.4에서 더 지연되였으며 약물 전달체의 함유량이 증가함에 따라 약물의 방출시간도 지연되었다. 피막된 고분자 매트릭스의 종류에 따라 지연된 약물의 방출시간은 키토산의 경우가 가장 길었으 며, 술폰화키토산, 키토산.염의 순셔였다. Monolith IC 고분자 매트릭스에 비해 2배 정도의 약물의 방출 지연성을 보인 피막된 monolithic 고분자 매트럭스 가 방출조절형 제제로서 더 바람직한 것으로 관찰되었다. 이러한 제형들은 초기 급격한 약물 방출속도의 변화를 억 제 하는 sustained release pattern 제 제 임을 확인할 수 있었다.

In this study, the release experiments of drug were operated in the phosphate buffer solutions of pH 7.4 and pH 1.2 by using drug carriers(chitosan, chitosan hydrochloride, and sulfonated chitosan)coated by poly(DL-lactide) with prednisolone for delivery drug. The release time of drug was more delayed in pH 7.4 than in pH 1.2. The release time of according to the kinds of drug carrier was delayed in the order of chitosan, sulfonated chitosan, and chitosan hydrochloride. In short, the formulation allows biodegradable coated monolithic polymetic matrices to suppress the burst effect of the drug release mechanism, which led to the sustained release pattern.

Glucose biosensor용 glucose oxidase 고정화 막의 단일화 벚 일회용의 간편화를 위한 연구로서, glucose oxidase의 고정화 대상막으로는 CTA와 PCL의 촌합비율이 80/20인 혼합막을 사용한 고저화 방법 중에서는 glutaraldehyde one-steD 방법으로 효소고정화층의 건조 전 두께가 10um 인 효소고정화 막이 효과적이었다. 이 고정화 막을 천자현미쇠 으로 관찰해 본 결과, 고밀도의 CTA/PCL 막층 위에 GOD-glutaraldehyde 층이 건조 후 3um 정도로 덮여 있는 것을 알 수 있었다. Dissolved oxygen전극을 사용하여 glucose농도의 증가에 따른 전류세기의 차이 값을 측정해 본 결과, glucose 7mM의 안도범위 내에서 선형성을 나타냈으므로 이 고정화원이 glucose sensor용 효소고정화 방법으로 가장 적합하였다. 한편, tyrosmase는 CTA와PCL의 혼 합비율이 80/20인 혼합막에 direct CDI 방법으로 고정화한 것이 가장 효과적이었으며, 8일 후에도고 정화된 tyrosmase의 활성이 35% 이상 유지되었다.

The disposable glucose bio-sensor using composite of CTA and PCL membrane was developed for measurement of glucose. The most effective membrane was composed of CTA/PCL(80/20, w/w) and glutaraldehyde one-step immobilization method (10um thickness) for glucose sensor gave the best result among various methods, considering oxygen permeability and electronic sensitivity. A scanning electron micrograph of the cross-section of a typical asymmetric CTA/PCL composite membrane showed that the membrane fused with a dense layer covered with a GOD-glutaraldehyde. Glucose oxidase immoblilized on the membrane showed the linearity between difference of absolute amperometric values and glucose concentrations within 7mM when the GOD immobilized electrode was used. About 35% of activity was remained after 8 days when the tyrosinase was immobilized on CTA/PCL (80/20) membrane.