Earticle

Due to its favor in human societies and continuous decline in production, studies on artificial cultivation of Tricholoma matsutake have been processed. As ectomtcorrhizal fungi, T. matsutake interacts with host plant, Pinus densiflora, by inhabiting in a fairy ring named shiro. In this study, relationship between T. matsutake and microorganisms from shiro was focused and total 1,397 fungal and bacterial species were isolated. Each isolates were co-cultured with T. matsutake mycelium from Yangyang and Bonghwa. Then growth area and three enzyme activities (α-amylase, cellulase, and β-glucosidase) of T. matsutake mycelium were analyzed, respectively. Among the isolates, total 4 fungal species and 6 bacterial species showed their promoting growth effect on T. matsutake mycelium. Discovery of these microorganism strains are expected to contribute on artificial cultivation of T. matsutake. This study was supported by the Rural Development Administration (PJ014766022023).

A new cultivar of oak mushroom ‘Jadam’ was bred from monokaryotic strains of ‘Hwadam-18’ and ‘GMLE36295-22’. The optimum temperature for mycelial growth of ‘Jadam’ on potato dextrose agar was 22-25℃. Total cultivation period of the new cultivar, from inoculation to its first harvest, was 122-124 days, shorter to that of the control cultivar‘Hwadam’. Total yield of ‘Jadam’ was 623.8 g per 3 kg substrate, and is higher than that of ‘Hwadam’(455.2 g). In the fruiting body of the new cultivar, the stipe was shorter and thinner than those of ‘Hwadam’. In farmhouse field test, it showed that the period of mycelial growth, browning were shorter than those of control cultivar ‘L808’. The total yield for 2 flush was 543.3 g for ‘Jadam’. It was similar to the 585.3 g of the control cultivar. Therefore, the new cultivar ‘Jadam’ could be a substitute for ‘L808’ in the field of farms. The effect of punching treatment on bags containing sawdust medium for the stable production of fruiting bodies of Lentinula edodes 'Jadam' was investigated. After spawn inoculation, the sawdust medium temperature and CO2 concentration reached 24.1℃ on the 33rd day of incubation, and on the 19th day of incubation, respectively, decreasing thereafter. The O2 concentration showed an opposite pattern to the CO2 concentration. As the number of punching treatments on the medium increased during the incubation period, the lightness value decreased, and conversely, the color difference and weight loss rate increased. There was no difference in the cultivation period according to the punching treatment. As the punching treatment increased on the sawdust medium, the fruiting body yield increased, When 15 holes are processed per bag with a diameter of 5mm and a depteh of 70mm, the yield for two cycles was the best with 644.7g. Thus, proper punching treatment on a sawdust medium during the incubation period can increase the number of fruiting bodies and yield.