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PCR-RFLP를 이용한 한우 Stearoyl-CoA Desaturase-1(SCD1)유전자의 SNP 및 경제형질 관련성 분석
강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 동물자원연구 제23권 1호 2012.06 pp.1-7
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4,000원
Stearoyl-CoA desaturase (SCD1) gene plays important role in fatty acid composition. In order to find marker-assisted selection (MAS) for improving the economic trait, this study was performed to identify the 878T>C single nucleotide polymorphism (SNP) on SCD1 gene. Three genotypes (CC, CT, and TT) were detected in 878T>C SNP of SCD1 gene from 103 Hanwoo population by polymorphism chain reaction-restriction fragment length polymorphism (PCR-RFLP) and economic traits were analyzed by general linear model. The frequency of allele C and T was 0.534 and 0.466, also the genotype frequency of CC, CT and TT was 0.252, 0.563, and 0.184, respectively in the Hanwoo population. The TT genotype of SCD1 gene showed a significantly higher measures (p<0.05) of carcass weight (CW) than CT, CC genotype. No significant association was detected between genotype and other economic trait (marbling, backfat thickness, and longissimus muscle area) in this study. The results revealed that SCD1 gene 878T>C SNP could be useful for effective MAS to increase the economic quality in Hanwoo population.
강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 동물자원연구 제23권 1호 2012.06 pp.8-11
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4,000원
This study was conducted to estimate heritabilities and genetic and phenotypic correlations among growth traits (weaning weight, weight of 12 month, weight of 18 month, weight of 24 month). Growth data collected from steers (n=3,321) and 8,818 animals in pedigree were used for estimation of genetic parameters. Traits evaluated included live weight at 4 month (W4), 12 month (W12), 18 month (W18) and 24 month (W24). Parameters were estimated using an multitrait animal model and derivative-free restricted maximum likelihood procedures. Heritabilities estimated for growth traits at W4, W12, W18 and W24 were 0.21, 0.11, 0.08 and 0.14, respectively. Genetic correlations were in the range of 0.79 to 0.87. Phenotypic correlations were in the range of 0.16 to 0.81.
쑥 분말의 첨가 급여가 육계의 간 지방산 조성 및 혈액 성분에 미치는 영향
강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 동물자원연구 제23권 1호 2012.06 pp.12-18
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4,000원
This study was carried out to investigate the effect of wormwood meal addition on constituent of blood, and liver fatty acid in broiler chickens. Two-day old 25 male commercial broiler chicks were distributed to 5 treatments (wormwood meal levels ; T1 (1%), T2 (3%), T3 (5%), T4 (10%), and control (C)) for 5 weeks. Free fatty acids of liver were a little different among the treatments and the palmitic, stearic, oleic, and linoleic acids were occupied 92.5% of total free fatty acids. Hemoglobin and hematocrite concentration of blood were significantly decreased (p<0.05) as the levels of wormwood meal increased, and serum albumin concentration of broilers was little different between 0 week and 5 week experiments. Also serum cholesterol, phospholipid, total cholesterol, and triglyceride concentration of liver in broilers were little different during the 5 weeks experiment. Serum triglyceride concentration was significantly increased (p<0.05) as levels of wormwood meal increased. These results indicate that dietary wormwood meal addition has a little positive effect on increasing unsaturated fatty acid and decreasing saturated fatty acid.
면실박의 급여가 비거세 한우의 도체 및 육질 특성에 미치는 영향
강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 동물자원연구 제23권 1호 2012.06 pp.19-25
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4,000원
The objective of this research was to investigate the effect of dietary cottonseed meal (CSM) supplementation on carcass and meat quality characteristics of Hanwoo (Korean cattle) bulls. Twenty two months-old-Hanwoo bulls of eight heads (4 heads per treatments) were fed for 5 months on a diet with or without (control) CSM (500 g/head/day). The samples of M. longissimus dorsi from carcasses were used for the quality measurement. Dietary CSM supplementation did not affect the carcass traits of bulls and pH value, drip loss, water-holding capacity and fatty acid composition of beef loin. Crude protein and crude ash content of beef loin were significantly (p<0.05) higher in beef loin from bulls fed CSM than in the control. During storage of 5 days at 4℃, CIE a*, b*, C* and total color values were significantly (p<0.05) higher in beef loin from bulls fed CSM than in the control. Overall, the present results indicate that dietary CSM supplementation may improve the oxidation stability of Hanwoo beef loin.
시판 식초류를 이용한 마리네이드 처리가 한우 대퇴두갈래근육질에 미치는 영향
강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 동물자원연구 제23권 1호 2012.06 pp.26-32
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4,000원
This study was investigated the effects of marinating beef with thirteen different commercial vinegars on quality traits of Biceps femoris (BF) muscle of Hanwoo. Samples were divided into thirteen portions and injected with vinegar which was equivalent to 10% of the sample weight. The samples were all marinated for 15 hrs at 4℃ for experiments. The pH and cooking loss were tended to decrease (p<0.05) on all the vinegars and swollen rate was increased only on pomegranate vinegar. Shear force was decreased on all the thirteen commercial vinegars except persimmon vinegar. Most of commercial vinegars had influenced on water holding capacity, excluding omija-persimmon, pomegranate and grape vinegars. The yield was the greatest with the aloe-apple (p<0.05). Redness (a) was stable on aloe-apple and omija-persimmon vinegars. With an improvement of experimental design and process for future work, the results could be suggested to industrial utilization wherein low-quality meat could be improved by simple marination.
강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 동물자원연구 제23권 1호 2012.06 pp.33-38
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4,000원
This study was performed to analyze the causative agents of disease occurred in Korean black goat. Bovine viral diarrhea (BVD) and infectious bovine rhinotracheitis (IBR) known as main causative agents of diarrhea and abortion in cattle were founded in Korean black goats. Escherichia coli and Clostridium perfringens are the main causes underlying diarrhea in Korean black goats as well as other animals. In addition, the orf virus was found as a causative agent of contagious ecthyma that shows frequent occurrence in Korean black goats. Leptospirosis was found in Korean black goats with low occurrence rate. However, unlike the reports from many other countries, we could not detect the antibodies for Johne’s disease and Neospora caninum in Korean black goats. These results are likely to contribute to improving the productivity of raising black goats as a field of livestock farming.
FMD(구제역)가 소비자의 국내산 축산물 구매패턴에 미치는 영향 - 춘천시를 중심 -
강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 동물자원연구 제23권 1호 2012.06 pp.39-49
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4,200원
This survey focused on consumer's consumption patterns for buying factors of domestic live stock products after foot and mouth disease (FMD). The purpose of this survey was to suggest basic information to reduce consumption when livestock diseases are occurred. Two methods were used for this survey. First method was to investigate internal data to understand consumption patterns when buying domestic livestock products. Second method included empirical analysis to understand how FMD affected consumer's consumption pattern. Survey was conducted in Chuncheon city. As the result of this survey, factors that affected the buying patterns of domestic livestock products were brand and expiration date. These results suggested that consumers recognized brand livestock products were safer than general livestock products and they have more interest for food safety than quality attributes of food when buying domestic livestock products.
소 유전체에서의 CNV(Copy Number Variation) 연구현황(총설)
강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 동물자원연구 제23권 1호 2012.06 pp.50-58
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4,000원
Since the Bovine sequencing and HapMap projects, there have been millions of genetic variations particularly including single nucleotide polymorphisms (SNPs) available throughout the cattle genome. While cataloguing substantial amounts of SNPs, copy number variation (CNV) has recently become getting great attention as an another form of genetic variation which is extensively distributed across the cattle genome. As it has been well known that CNVs can account for substantial proportions of phenotypic variance in other species including human, CNVs are highly expected to explain genetic variations of diverse economically important traits in cattle. Currently, two main approaches such as hybridization-based microarray and massively parallel sequencing-based method have been successfully applied to detect CNVs throughout the cattle genome. Although there is obvious difference of capabilities to detect genome-wide CNVs at fine scales between different platforms applied, all currently completed investigations exhibited that CNVs are extensively spread throughout the cattle genome as observed in other species. Despite these successes, there are still severe lacks of researches to identify multiple levels of CNVs from diverse cattle or multiple individuals, suggesting that there are a number of CNVs remained undiscovered. Furthermore, there are almost few investigations available to elucidate how each CNV is associated with traits of interest in cattle, so such functional study should be followed along with saturating most of potential CNVs on cattle genome.
강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 동물자원연구 제23권 1호 2012.06 pp.59-73
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4,800원
Transgenic pigs are promising donor organisms for xenotransplantation as they share many anatomical and physiological characteristics with humans. Recently, a step has been moved closer to xenotransplantation by producing genetically modified pigs that has no α-1,3-Gal epitope, the major xenoantigens triggering HAR of pig to primate xenografts. Further genetic modifications such as expression of human complementary regulatory proteins, CD39, endothelial protein C receptor, heme-oxygenase 1, thrombomodulin, tissue factor pathway inhibitoras well as modulators of the HLA-E/β-2-microglobulin, and CTLA-4Ig are due to address for further rejection mechanisms and incompatibilities between porcine and primate blood coagulation systems. Although the pig is the favored species for use as a xenograft donor, a detailed description of the transgenic pig development and surgical technique is lacking which seems mandatory to address for broader understanding of this issue.
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