Earticle

벼 약(葯) 배양(培養)에 있어서 이삭내(內), 이삭당(當) callus의 형성량(形成量)의 차이(差異)와 형성(形成)된 callus의 처리과정(處理過程)의 분화(分化)에 미치는 영향(影響), 저온처리(低溫處理)에 의한 callus 형성량(形成量)의 증가(增加)와, 분화(分化)에서의 효과(效果)를 구명(究明)하기 위해 실시(實施)한 시험(試驗)의 결과(結果)는 다음과 같다. Callus의 행장(生長)에는 고체배지(固體培地)가 액체배지(液體培地)보다 양호(良好)하였고, 특히 N-6배지(培地)가 19.8배(陪)의 생장율(生長率)로 가장 양호(良好)하였다. 분화(分化)에서도 역시 N-6배지(培地)가 뿌리에서 37.50%의 분화율(分化率)로 가장 높았으며, P. E 배지(培地)는 callus생장(生長)에는 M-S배지(培地)보다 불량(不良)하였으나 분화율(分化率)은 5% 높았다. 한 이삭내(內)에서는 극히 제한(制限)된 수(數)의 약(葯)에서만 callus가 형성(形成)되었으나 그 분포(分布)는 넓은 편이었다. 저온처리(低溫處理)로서 callus 형성량(形成量)이 증가(增加)하였고 특히 에 5일간(日開) 처리(處理)한것은 처리(處理)하지 않은것보다 38%가 증가(增加)하였다. 또한 저온처리(低溫處理)로서 callus의 형성시기(形成時期)가 빨라지며 형성기간(形成期間)의 폭(幅)도 넓어지는 경향(傾向)이 있다. 약(葯)에서 형성(形成)된 callus는 빨리 분화배지(分化培地)에 이식(移植)하는 것이 분화율(分化率)이 높으며 시일(時日)이 경과(經過)할수록 분화율(分化率)이 감소(減少)하였다. 고농도(高濃度)의 2, 4-D는 Callus의 생장과정(生長過程)에서 분화능력(分化能力)을 유지(維持)시켜 주는것으로 추정(推定)되며 분화중(分化中)의 callus는 저온처리(低溫處理)로서 뿌리에는 급격(急激)한 분화율(分化率)의 감소(減少)를 야기(惹起)시키나 줄기에는 에 5일간(日間) 처리(處理)한 것이 유효(有效)하였다.

This study was conducted to obtain basic information on the rice anther culture. Materials used were (Inabawase X YR 2404-14-2-1) hybrid. Callus growth rate on various media, induction frequency of callus in spikelet and panicle, and the effect of treatment on anther and callus were evaluated. The results obtained were summarized as follows ; The growth rate of callus on N-6, M-S, P.E.agar media was 19.8, 13.1, 4.1 times respectively after 30 days inoculated, and on liquid media was 3.8, 5.1, 1.4 times, respectively. Organ differentiation on N-6, M-S, P.E.agar media was 37.5%, 12.5%, 17.5% respectively. The difference of induction frequency of callus per panicle was 0.14%-6.25% and per spikelet was 0-19.05%. Almost callus was induced 30-35 days after inoculation. Organ differentiation of induced callus was decreased by culture. Callus cultured for 13 days after induction did not make shoot. Anthers cold shocked at for 5 days obtained 3.32% efficiencys of callus induction per number of anthers plated, and compared with 2.41% of no treated anthers. But anther treated at for 7 days decreased 2.24%. Callus induction periods were shortened by cold treatment for about 5 days. Callus cultured on medium containing 2 mg/l of 2, 4-D showed 5% on root formation but medium containing 5 mg/l of 2, 4-D showed 30% of root formation after transfered on the medium without 2, 4-D. Callus cold shocked at revealed poor efficiency for root formation, but 5 days treatment was good for shoot formation.

건과육중(乾果肉重)(또는 건과육율(乾果肉率)이 상대적(相對的)으로 많은 건과용(乾果用) 고추를 육성(育成)하는데 기초자료(基礎資料)를 얻고자 8개친(個親)과 이면교배(二面交配)한 28조합(組合)을 공시(供試)하여 1건과중(乾果重)에 대(對)한 구성요소(構成要素)의 비율(此率)을 조사(調査)하고 과경중(果梗重), 태좌중(胎座重), 종자중(種子重) 및 과육중(果肉重)의 잡종강세(雜種强勢), 조합능력(組合能力) 및 유전(遺傳) 분석(分析)을 한바 얻어진 결과(結果)는 다음과 같다. 친(親)과 에 있어서 1건과중(乾果重)과 구성요소(構成要素)들은 초기과(初期果)가 중기과(中期果)에서 보다 모두 무거웠다. 그리고 초(初), 중기과(中期果)에 있어서 이들은 모두 친간(親間), 간(間)에 1%의 유의(有意)를 나타내었고, 이 친(親)보다 무거웠으나 초기과(初期果)의 친(親)은 중기과(中期果)의 보다 무거웠다. 1건과중(乾果重)에 대(對)한 구성요소(構成要素)들의 비율(比率)은 과육(果肉)이 가장 높고 다음은 종자(種子)였다. 과육율(果肉率)은 초기과(初期果)보다 중기과(中期果)에서 다소(多少) 증가(增加)되었고 종자율(種子率)은 상대적(相對的)으로 감소(減少)되었으며 과경(果梗)과 태좌(胎座)는 수확시기(收穫時期)에 따른 차이(差異)가 없었다. 무거운 친(親)을 초월(超越)하는 조합(組合)이 상당(相當)히 있었으며 평균(平均)heterosis(%)는 모두 정(正)의 값을 나타내었고 평균(平均)heterosis(%)는 종자(種子)와 1건과중(乾果重) 외(外)는 모두 부식(負植)를 나타내었다. GCA는 SCA는 모두 1%의 유의성(有意性)을 나타내었으며 GCA가 SCA보다 컸다. 무거운 쪽이 모두 우성(優性)을 나타내었고 과경(果梗)은 부분우성(部分優性), 태좌(胎座), 과육(果肉) 및 1건과중(乾果重)은 완전우성(完全優性), 종자(種子)는 초우성(超優性)을 타나내었다. 유효유전자수(有效遺傳子數)는 과경(果梗)과 태좌(胎座)는 1개(個), 종자과육(種子果肉) 1건과중(乾果重)은 2개(個)로 추정(推定)되었으며 유전력(遺傳力)은 모두 높았다.

This study was performed to obtain the basic informations for red dry pepper fruit with more pericarp weight(or in percentage) with a complete diallel cross(excluding reciprocals) using eight cultivars. Heterosis, combining ability and inheritance of the dry red fruit weight and its components(stem, placenta, seed, and pericarp) were evaluated. The results obtained were summarized as follows : Dry weight/fruit and its four antomical components were heavier in the earlier harvest fruit than in that of the later fruit. They showed 1% significance among parents and , and those of were significantly heavier than in parent. All characters in earlier fruit of parent, however, were higher than in later fruit of . Dry weight percentage of pericarp to dry weight/fruit was highest followed by seed. Percentage of pericarp in the later fruit was increased while the seed decreased and percentages of stem and placenta were not differed between the earlier and later fruit. hybrids above the higher parent were observed in all characters. Mean heterosis (%) was positive in all characters while mean heterobeltiosis (%) was negative excepting seed and dry weight/fruit. GCA and SCA variances were highly significant, and GCA vaiances were greater than SCA in all characters. The directions of dominance were positive. Partial dominance was shown in stem, complete dominance in placenta, pericarp and dry weight/fruit, and over dominance in seed. The effective genes were estimated as one for stem and placenta, and two for seed, pericarp and dry weight/fruit. Heritabilities in narrow and broad sense were higher.

동할발생조건(胴割發生條件)에 대(對)한 미립(米粒)의 형태(形態)나 성분함량(成分含量)의 영향(影響)을 알고저 비료시용방법(肥料施用方法)을 달리하여 재배(裁培)한 벼의 동할립(桐割粒)과 건전립(健全粒)을 구분(區分)하여 분석(分析)해본 결과(結果) 동할발생(桐割發生)은 삼강(三剛)벼가 락동(洛東)벼보다, 만기수확구(晩期收穫區)가 적기수확구(適期收穫區)보다 많았으며 3요소외(三要素外)에 규산질비료(珪酸質肥料)를 병용함으로서 동할율(桐割率)이 감소(減少)되었다. 동할립(桐割粒)은 건전립(健全粒)에 비(比)하여 천립중(千粒重) 및 미립(米粒)의 체적(體積)(립장(粒長)립폭(粒幅)립후(粒厚)), 립장(粒長), 립장(粒長)/립폭(粒幅)이 컸으며 품종간(品種間)에는 미립(米粒)의 립장(粒長)/립폭비(粒幅比)가 큰 삼강(三剛)벼가 락동(洛東)벼보다 동할율(胴割率)이 높았다. 동할(胴割)이 비교적(比較的) 잘 되는 삼강(三剛)벼는 미립내(米粒內) 규산함량(珪酸含量)이 낮았고 인산함량(燐酸含量)이 높았으며 동할(胴割)이 잘 안되는 락동(洛東)벼는 그 반대(反對)이었다. 그래서 삼강(三剛)벼는 미립내(米粒內) 규인비(珪燐比)가 낮았고 락동(洛東)벼는 높았으며 비료삼요소(肥料三要素)에 규산(珪酸)을 시용(施用)함으로서 그 함량비(含量比)를 높일수 있었다.

To investigate the influences of morphological features and the chemical compound of rice grain on the crack of rice kernels, rice was cultivated under the different method of fertilizer application and was harvested at optimal and late stage to the crack features of kernels of cracked and healthy grains. The results are summarized as follows ; The occurrence of cracked kernel was more severe in "Samgang-byeo" than in "Nagdong-byeo" and the rice harvested at the late stage was more cracked kernel than that of optimal harvest. The application of silicate fertilizer in addition to the N.P.K. fertilizer resulted in the decrease of cracked kernels. The grain weight, the grain volume (length width thickness), grain length and grain length/grain width etc. of the cracked kernels were larger than those of the healthy grains. The long "Samgang-byeo", having the long grain Shape, which has larger ratio of grain length/grain width than that of "Nagdong-byeo", shows higher rate of cracked kernels. The grain of "Samgang-byeo" which is easily cracked relatively it contains lower silica and higher phosphorus while "Nagdong-byeo" shows the opposite results. The ratio of silica/phosphorus in the grain was low in "Samgang-byeo" but it was high in "Nagdong-byeo". The ratio of silica/phosphorus in rice grain was increased by the application of silicate fertilizer in addition with N.P.K. fertilizers.

소맥(小麥) 집단(集團)에 있어서 조숙(早熟) 다수성(多收性) 계통(系統)의 효율적(效率的)인 선발(選拔)에 관(關)한 기초자료(基礎資料)를 얻고자 중숙(中熟)조숙(早熟)(올밀/새밀), 만숙중숙(中熟)(Naphal/청계밀) 두 조합(組合)을 공시(供試)하여 집단(集團)의 유전적(遺傳的) 변이(變異)를 조사(調査)하고 경로분석(徑路分析)을 한바 얻어진 결과(結果)는 다음과 같다. 유전변이계수(遺傳變異係數)는 올밀새밀 조합(組合)에서는 수획지수(收獲指數), 립수(粒數), 수량(收量), 간장(稈長)의 순(順)으로, Naphal청계밀 조합(組合)에서는 수획지수(收獲指數), 간장(稈長), 1,000 립중(粒重)의 순(順)으로 높았다. 유전력(遺傳力)(협의(狹意))은 양조합(兩組合)에서 출수기(出穗期), 성숙기(成熟期), 간장(稈長), 수획지수(收獲指數)가 높았고(0.525~0.808) 립수(粒數), 수량(收量)은 낮았다. 유전획득량(遺傳獲得量)(%)은 출수기(出穗期), 성숙기(成熟期)가 양조합(兩組合)에서 모두 높았고 수량(收量)은 낮았다. 1,000립중(粒重)의 유전획득량(遺傳獲得量)(%)은 Naphal청계밀 조합(組合)에서는 높게 나타났다. 수량(收量)과 수수(穗數) 및 립수(粒數)와는 높은 정(正)의 유의상관(有意相關)을 보였고 수량(收量)에 대(對)한 직접효과(直接效果)는 수수(穗數)가 가장 컸고 다음은 립수(粒數)였다.

This study was conducted to obtain the basic informations on the selection for the early and high-yielding lines in populations of wheat. Two combinations such as Olmil-Saemil cross and Naphal-Cheonggemil cross and their eight populations were used and genetic parameters of genetic variances and path-coefficients for agronomic traits mainly in populations were analyzed. Genetic coefficient of variability in Olmil-Saemil cross was higher in harvest index, grain number, grain yield and plant height in decreasing order, but in Naphal-Cheonggemil cross harvest index, plant height, and 1,000-grain weight were higher in order. Heading and maturing dates, plant height, and harvest index showed high hertability (0.525-0.808) in both crosses, but very low heritabilities were observed in grain numbers per panicle and yield. In both crosses, genetic advances(%) was higher in heading and maturing stage, but lowest in yield. High genetic advance was obtained in 1,000-grain weight of Naphal-Cheonggemil cross. The highly significant correlations between yield and panicle number, yield and grain number per panicle were obtained, and the highest direct effect on yield was obtained in panicle number followed by grain number per panicle number.

식물생장조절제(植物生長調節劑)에 의(依)한 담배의 액아발생(腋芽發生)에 관(關)한 기초자료(基礎資料)를 얻고자 조생종(早生種)인 hicks와 만생종(晩生種)인 kusaga mammoth에 MH, GA, BA의 농도를(濃度)를 달리해서 단독(單獨) 또는 혼용처리(混用處理) 하였을 시 액아발생(腋芽發生)에 미치는 영향(影響)을 구명(究明)한바 그 결과(結果)를 요약(要約)하면 다음과 같다. 적심(摘心) 직후(直後) MH900g(a.i.)/ha 처리(處理)는 액아발생(腋芽發生)을 완전히(完全)히 억제(抑制)시켰지만 농도(濃度)가 감소(減少)함에 따라 품종(品種)에 관계(關係)없이 액아(腋芽)가 발생(發生)하였다. 무적심(無摘心) 개체(個體)에서도 비슷한 경향(傾向)을 나타내었다. 적심(摘心)한 개체(個體)에서는 GA와 BA의 여느 농도(濃度)에서 혼용(混用)을 하여도 MH가 첨가(添加)되면 액아(腋芽)가 발생(發生)하지 않지만 무적심(無摘心) 개체(個體)에서는 MH 900 g(a. i.)/ha에 BA와 GA의 농도비(濃度比)가 10:1로 혼용(混用)될 때는 액아(腋芽)가 발생(發生)하였다. 무적심(無摘心) 개체(個體)에 GA와 BA 처리(處理)는 액아발생(腋芽發生)에 아무런 영향(影響)을 미치지 않았다. 적심(摘心)한 개체(個體)에 GA와 BA의 단독처리(單獨處理)는 농도(濃度)가 높아짐에 따라 액아발생(腋芽發生)을 증가(增加)시키며 혼용(混用)하였을 때에는 antagonistio한 경향(傾向)을 나타내었다.

This study was conducted to obtain basic informations for growth regulators on occurrence of sucker in tobacco plants (Nicotiana tabacum L.). Varieties used were "hicks" and "kusaga mammoth" and growth regulators such as MH(maleic hydrazide), GA(Gibberellic acid) and BA(Benzyl adenine) were used. Immediately after topping, an application of maleic hydrazide at 900g a.i./ha completely inhibited sucker development, but sucker were developed as the rates of MH decreased, in both varieties. In nontopped tobacco plants, the similar trend as in the topped plant was observed except for no sucker development in the untreated control. Any combination of GA and BA under presence of MH had no effects on sucker development in the topped tobacco plants. However, in the nontopped plants, sucker were observed when the combined ratio of BA and GA was 10 to 1 under the presence of MH standard level. The highest no. of sucker was obtained when combined BA with GA under the presence of MH, showing higher response of hicks than that of kusaga mammoth. A single application of GA and BA in the topped plants markedly increased sucker number as GA concentrations increased showing varietal difference. GA increased sucker number as high as as 42% for hicks, but inhibitory effect on kusaga mammoth in comparision with the untreated control, showing very effective on hicks. BA showed the similar effect like GA. Combinations of GA and BA showed antagonistic effect on sucker development. The length of sucker was markedly promoted as the GA rates increased, and the promotive effect of sucker length by GA was not nullified by the addition of BA. But combination treatment of GA and BA mostly resulted in less dry weight than the untreated control, indicating that sucker developed from the combined treatments of GA and BA were not normal and kusaga mammoth was more affected by them.

땅콩 기생선충의 종류(種類) 및 그 방제법(防除法)을 구명(究明)코자 1980년(年)부터 1981년(年)까지 2개년(個年)에 걸쳐 실시(實施)된 결과(結果)를 요약(要約)하면 다음과 같다. 1. 땅콩 기생선충으로는 Aphelenchoides besseyi, Aphelenchus avenae, Criconemoides informis, Helicotylenchus dihystera, Meloidogyne arenaria, Meloidgyne hapla, Psilenchus hilarulus, Pratylenchus minyus, Tylenchus sp. 등(等) 9 종(種)이 동정(同定)되었다. 2. 약제처리(藥劑處理)에 따른 선충의 계절적(季節的) 변화(變化)는 처리(處理) 90일후(日後)에는 밀도(密度)가 낮았다가 170일후(日後)인 수확기(收穫期)에는 다시 증가(增加)되는 경향(傾向)을 나타냈으며 당근뿌리흑선충(Meloidogyne hapla)의 밀도(密度)는 수확기(收穫期)에 있어서도 훈증제(薰蒸劑)인 Telone C-17 처리구(處理區)는 무처리구(無處理區)에 비(比)하여 현저(顯著)히 감소(減少)되었으며 1%의 유의성(有意性)이 인정(認定)되었다. 3 초장(草長), 분기수(分技數), 건물중(建物重)은 Telone C-17 처리구(處理區)가 가장 높았으며 그다음 Mocap 10G, Carbofuran 3 G. 등(等)의 순(順)이었다. 4. 수량(收量)에 있어서는 Telone C-17 처리구(處理區)가 무처리구(無處理區)에 비(比)하여 고령(高靈)에서는 73% 선산(善山)에서는 60%가 증수(增收)되어 가장 효과(效果)가 좋았고 다음이 Mocap 10G. Carbofuran 3G. 등(等)의 순(順)으로 나타났다.

Nine species of plant parasitic nematodes, Aphelenchoides besseyi, Aphelenchus avenae, Criconemoides informis, Helicotylenchus dihystera, Meloidogyne arenaria, Meloidogyne hapla, Pratylenchus minyus and Tylenchus sp. Were found in peanut field in Korea. Chemicals used were; Telon C-17, Mocap 10G and Carbofuran 3G for control peanut parasitic nematodes. All chemicals reduced nematode populations but varied in effectiveness. Telon C-17 was especially effective against Meloidogyne hapla, the principal species on peanut and resulted in significant yield increased than the control. Plant height, number of branches and dry weight of peanut were increased over the nontreated control by chemical soil treatments.

A strain of Alcaligenes sp. Isolated from the sludge of industrial area was found to uptake 24.1 mg lead/g dried cell during incubation in medium containing 100g/ml of lead. Analyses of cellular subfractions reveal that fractions of cell wall contain 88.6 percent of lead found associated with the cells and the remainder is found associated with the cytoplasmic fraction. Ultrastructural examination of the cells cultured in media containing 500 and 1000g/ml of lead showed no major irregularities between cells of the treated and untreated cultures.

본(本) 시험(試驗)은 제사용수(製絲用水)에 대(對)한 몇가지 화학약제(化學藥劑)의 처리(處理)가 견층(繭層) sericin의 용해성(溶解性)에 미치는 영향(影響)을 조사(調査)하였던 바, 견층(繭層) sericin의 용해량(溶解量) 조절(調節)에 참고자료(參考資料)가 될 수 있는 다음과 같은 결과(結果)를 얻었다. 공시(供試) 약제(藥劑) 중(中)에서 견층(繭層) sericin의 용해촉진력(溶解促進力)이 가장 큰 약제(藥劑)는 Sodium Hydroxide(NaOH)였으며, Potassium Hydroxide(KOH) > Sodium Silicate() > Sodium Bicarbonate()의 순(順)으로 용해추진력(溶解促進力)이 작았다. 공시(供試) 약제(藥劑) 중(中)에서 견층(繭層) sericin의 용해억제력(溶解抑制力)이 가장 큰 약제(藥劑)는 Form Aldehyde(HGHO)였으며, Ammonium Alum() > Acetic Acid() > Hydrochloric Acid(HCl)의 순(順)으로 용해억제력(溶解抑制力)이 작았다. 공시(供試) 약제(藥劑) 중(中)에서 견층(繭層) sericin의 용해억제력(溶解抑制力)이 클 것으로 기대(期待)했던 Hydrochloric Acid(HCl)은 이상(以上)의 고온(高溫)에서는 오히려 견층(繭層) sericin의 용해(鎔解)를 크게 촉진(促進)함을 알 수 있었다.

The purpose of this study is to find out influences of several chemicals on the solubility of cocoon sericin in water, and there are several results of use to control the solubility of cocoon sericin in water. The results obtained are summarized as follows : The chemical which shows the strongest accelerating power of the solubility of cocoon sericin in water is Sodium Hydroxide (NaOH), the second is Potassium Hydroxide (KOH), the third is Sodium Silicate (), and the weakest is Sodium Bicarbonate () in order among noticed silk-reeling accelerators. The chemical which shows the strongest inhibiting power of the solubility of cocoon sericin in water is Form Aldehyde (HCHO), the second is Ammonium Alum (), the third is Acetic Acid (), the weakest is Hydrochloric Acid(HCl) in order among noticed silk-reeling inhibitors. Particulary Hydrochloric Acid (HCl), which is expected to show strong inhibiting power of the solubility of cocoon sericin in water, shows accelerating power of the solubility of cocoon sericin at high temperature over 80 degrees of Celsius thermometer in water.

1980년(年) 한국(韓國)에서 발견(發見)된 Microsporidia S 80은 포자(胞子)의 길이가 폭(幅)이 으로 난원형(卵圓形)인데 그 크기가 형태(形態)로 보아 누에에 기생(寄生)하는 Microsporidia로서 지금까지 한국(韓國)이나 일본(日本)에서 알려진 것 들과는 다른 것이다. 과산화수소(過酸化水素) 원액(原液)으로 처리(處理)하였을때에 추출(抽出)된 극사(極絲)의 길이는 평균(平均) 이었고 극사(極絲)의 선단(先端)에서 둥근 모양의 sporoplasm이 돌출(突出)되어 있는 것이 관찰(觀察)되었다. 한편 포자(胞子)는 Giemsa, Safranin-O, Gram 등(等)의 염색(染色)에서 부분적(部分的)으로 염색(染色)되는 특징(特徵)이 나타났으며, 전자현미경(電子顯微鏡)으로 관찰(觀察)한 포자(胞子)의 미세구조(微細構造)는 제일 바깥 부분(部分)이 전자밀도(電子密度)가 높고 요철(凹凸)이 있는 얇은 exospore로 둘러싸여 있고 그 안쪽에는 투명(透明)하고 두꺼운 endospore가 있는데 endospor는 포자(胞子)의 정단부(頂端部)가 다른 부위(部位)보다 상당히 얇은데 이곳으로 극사(極絲)가 추출(抽出)되는 것으로 생각된다. endospore와 cytoplasm과의 경계부(境界部)에는 세포질(細胞質)을 싸고 있는 한층(層)의 한계막(限界膜)인 inner limitting layer가 있다. 세포질(細胞質)의 전단부(前端部)에는 polar cap이 있고 그 밑에는 lamellar 구조(構造)의 발달(發達)된 polaroplast가 있으며 polaroplast의 후부(後部)는 vesiculate part로 되어 있는 것으로 보인다. polar filament는 anchoring disc인 polar cap에서 곧게 뻗어 나아가다가 포자(胞子)의 내벽(內壁)을 따라 돌면서 용수철 처럼 말려 있는데 polar filament의 coiled part 중앙(中央)에 2중막(重膜)에 싸여진 전자밀도(電子密度)가 높은 후형질(厚形質)로된 2핵(核)이 인접(隣接)하여 있으며 포자(胞子)의 부단부(部端部)에는 posterior vacuole이 있을 것이다. 이러한 포자(胞子)의 크기와 형태(形態) 및 미세구조(微細構造)로 보아 Microsporidia인 것을 확인(確認)할 수 있으며 분류학(分類學) 상(上)의 위치(位置)는 Microsporea 강(綱), Microsporidia 일(日)에 소속(所屬)시킬 수 있고 아목(亞目) 및 속(屬)을 규명(糾明)하기 위(爲)해서는 그 생활사(生活史)가 밝혀져야 한다. Mcrosporidia S80을 2령(齡) 기잠(起蠶)에 경구접종(經口接種)한 경우(境遇)의 LD50은 로서 인 Nosema bombycis와 비교(比較)할때 병원성(病原性)이 낮은 것으로 나타났으나 그 발생지역(發生地域)이 경기도(京畿道)를 중심(中心)으로 인접지역(隣接地域)인 강원(江原), 충북(忠北) 충남(忠南) 및 전남(全南)에서도 발생(發生)되고 있는 점(點)과 4령기(齡期) 접종시(接種時) 전견중(全繭重)과 견층중(繭層重)에 나쁜 영향(影響)을 미칠뿐 아니라 화아비율(化蛾比率)이 낮고, 감염(感染)된 모아(母蛾)는 물론 감염(感染)된 웅아(雄蛾)와 교미(交尾)한 모아(母蛾)에서도 산란불능아(産卵不能蛾)가 발생(發生)하거나 산란수(産卵數)가 건전구(健全區)에 비(比)하여 떨어지는 동시(同時)에 부수정란(不受精卵) 비율(比率)도 높은 것으로 볼 때 사견양잠(絲繭養蠶)은 물론 종견양잠(種繭養蠶)에 있어서도 피해(被害)를 입고 있을 것으로 본다. 감염모아(感染母蛾) 21마리에서 얻은 차대잠(次代蠶)의 검사결과(檢査結果) 경란전달(徑卵傳達)은 되지 않는 것으로 나타났으나 혹시 경란전달(徑卵傳達)이 된다고 해도 그 빈도(頻度)는 매우 낮을 것이며 1/21 미만(未滿)일 것으로 생각된다.

The new microsporidia S80 isolated from, Bombyx mori L. in Korea showed ovoid in the morphology of the spores and the size were measured in length and width. No other microsporidian spore like this has not been so far isolated from Silkworm. The length of the polar filament extruded in hydrogen peroxide () at was of a round cytoplasm on the top. The spores were partly stained with Giemsa, Safranin-O and Gram as the same staining properties as Nosema bombycis, Microsporidia K 79 and other microsporidian spores. The fine structures were observed under scanning eleceron microscope through ultrathin sectioning. The spore wall was composed of three layers ; the thin exospore of an electron dense rippled layer, the thick electron lucent endospore which was thinning considerably at the polar filament insertion point, and the inner limiting membrane. Polar cap present at the sporeapex, with a long polar filament of 12-13 coils, subtending angle of to spore axis, which is tubular made up of a multilayered and are a benes core, light ring structure enclosing the dance core, the dark ring structure enclosing the inner light ring structure and the other than and light ring structure bounded from cytoplasm. Lamellate polaroplast occupied the anterior part of the spore, and the two neclei with dense nucleoplasm bounded by a double nuclear envelope were cited in the slight downer middle portion of spore. From the characteristics of the shape, size and fine structures, it is certain to reason the Microsporidia S80 belong to the phylum Microspora, class Microspora, order Microsporida, order Microsporida. The shape of two nuclei cited seems to be genus Nosema, but in the classification for the suborder it should be defined wheather pansporoblasts be formed or not and for the genis especial attempts have been made to define the characters which distinguish the disporous genera in the life cycle. Survey through the infection of the bad cocoons during 1980 to 1982 in South Korea the areas contaminated with new microsporidia were revealed 5 provinces of Kyung-Gi, Kang-Won, Chung-Nam and Chun-Nam. Pathological effects inoculated per os at second instar larvae of silkworm, the LD 50 was as lower pathogenecity than that of Nosema bombycis Naegeli of . While on the other hand the inoculation of the microsporidia at fourth instar larvae lowerd the whole cocoon weight and cocoon shell weight and significant at 1% level. The microsporidia S80 defined it can not be transmitted transovarially from the result of predictive and collective examination of 21 egg batches from the infected female moth.

경북(慶北)의 낙동강수계(洛東江水系)와 동해안수계농촌(東海岸水系農村) 17개(個) 지점(地点)을 기준(基準)하여 농업용수원(農業用水源)의 수질환경(水質環境)을 조사검토(調査檢討)하여 장래(將來)의 방재대책(防災對策)에 참고하고져 조사분석(調査分析)을 실시(實施)한 바 농업용수원(農業用水源)을 기준(基準)할 때 수온상태(水溫狀態)는 수질(水質)을 저해(沮害)시킬 큰 요인(要因)이나 문제점(問題点)이 없으며 PH는 6.5~7.8의 절위에서 기준치(基準値)에 저촉되지 않으며 염도의 기준(基準)이 되는 전기전도도(電氣傳導度)도 에 이르는 지점도 있으나 농업용수의 수질저해지점에는 미치지 못하며 BOD의 경우는 기준치 10ppm에 육박(肉迫)하는 지점(地点)이 있어 다소(多少)의 위험위협을 받고 있다. 그리고 DO의 경우는 위험기준치에 미달(未達)하고 있다. 그리고 중금속(重金屬)에 속하는 Cu, Pb, Cd 등도 아직은 위험기준치에는 미달(未達)이나 장차에는 주위의 산업(産業)과 도시발달(都市發達)에 따르는 특별(特別)한 조치가 없는 한(限) 위험조건에 이를 것으로 생각된다.

Total annual water requirements for agricultural use are exceedingly large. In many areas of the country, particularly in the arid regions, irrigation water requirements comprise the bulk of the total developed water supply. On the other hand, we are, at present, poorly prepared to evaluate the hazards of many substances in the water phase of the agricultural environments. This study is to survey the water quality phase of agricultural conditions and to give some ideas to prevent disasters from the environmental hazards such as PH, Electric Conductivity, BOD, DO, Cu, Pb, and Cd. In the analysed results, mean PH was 6.5 to 7.8, maximum EC was at , the maximum BOD was less than 10 ppm (standard value), the DO was 4.2 to 12.0 mg/L (standard value is more than 2.0 mg/L) and the Cu, Pb, Cd were estimated in scope of safety value respectively. Especially BOD is gradually closed with the hazardous point in the few places. At present, the hazardous environmental conditions originated from the municipal sewage, industrial waste, and human and domestic animals excrements etc. have to strongly be improved much better than before.

농기계(農機械)의 포장작업시(圃場作業時) 주행성능(走行性能)에 크게 영향(影響)을 미치는 토양경도(土壤硬度)를 깊이별로 측정(測定)하기 위한 토양경도계(土壤硬度計) 개발(開發)을 목적(目的)으로 스트레인 게이지를 이용(利用)한 토양경도계(土壤硬度計)를 제작(製作)하여 경도(硬度)를 측정(測定)한 실험(實驗)에서 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. Strain gage를 이용(利用)한 토양경도계(土壤硬度計)의 Calibration곡선(曲線)이 상관계수(相關係數)가 0.99876, Dial gage를 이용(利用)한 기존(旣存) 시험기(試驗器)의 Calibration 곡선(曲線)이 상관계수(相關係數)가 0.97150으로 나타나 Strain gage를 이용(利用)하는 쪽이 더욱 정밀(情密)한 측정(測定)이 가능(可能)하였다. 동일(同一) 토양(土壤)에 대(對)한 측정치(測定置)의 표준편차(標準偏差)는 모래, 논흙 2종(種)의 토양(土壤)에 대(對)하여 각각(各各) Strain gage를 이용(利用)한 토양경도계(土壤硬度計)가 6.794, 8.271, Dial gag를 이용(利用)한 기존(旣存) 시험기(試驗器)가 7.490, 9.161로 나타나 Strain gage를 이용(利用)하는 쪽이 측정오차(測定誤差)가 작았다.

A soil hardness meter with strain gages was developed in order to measure the hardness of the soil. Soil hardness tests were conducted at the laboratory based on this measuring system. Also, these results were compard with that from the cone penetrometer which is widely used for this purpose. The following conclusions were drawn from the results. 1. Since the correlation coefficient of calibration curve obtained from the soil hardness meter with strain gages was 0.99876 and that of calibration curve obtained from the cone penetrometer with dial gage was 0.97150, the soil hardness meter with strain gages was more recomendable than that with dial gage for this purpose. 2. Standard deviations of soil hardness for sands and soil of paddy field when the soil hardness meter with strain gages was used were 6.794 and 8.271, respectively and that of soil hardness for sands and soil of paddy field when the cone penetrometer with dial gage was used were 7.490 and 9.169, respectively. Thus, the soil hardness meter with strain gages seemed to have lower measuring error than with dial gage.

연습림(演習林)에 분포(分布)된 식물(植物)은 79과(科) 188속(屬) 248종(種) 16변종(變種), 1아종(亞種), 3품종(品種)으로서 총계(總計) 298종류(種類)였다. 목본식물(木本植物)은 39과(科), 67속(屬)112종(種), 7변종(變種) 3품종(品種)이었으며 이중에서 교목(喬木)이 50종류(種類) 관목(灌木)이 46종류(種類), 만목(蔓木)이 16종류(種類)였다. 참취, 더덕, 지치등의 식용식물(食用植物)이 118종류(種類) 오미자, 오갈피나무등의 약용식물(藥用植物)이 48종류(種類)였다. 이 지역일대(地域一帶)에서 아직 보고(報告)되지 않았던 특기할 수 있는 군락(群落)은 왕느릅나무(Ulmus macrocarpa), 오미자(Schizandra chinensis), 오갈피나무(Acanthopanax sessiliflorus), 지치(Lithospermum erythrorhizon)등이었다.

This experiment was conducted to investigate the flora of Kyungpook National University Forests, which is located in Walme-Ri, Hyundong-Myon, Chungsong-Kun and at 59' 01' 30"E longitude and 10' 13' 00"N latitude. The results obtained can be summarized as follows; The kinds of the vascular plants identified are 298 which are composed of 79 families, 188 genera, 248 species, 1 subspecies, 46 varieties and 3 formae. Woody plants are composed of 39 families, 67 genera, 112 species, 7 varieties and 3 formae. The total number of woody plants listed are 122, tall trees of woody plants 50, shrubs 46 and winders 16, respectively. The kinds of edible plants listed are 118 such as Lithospermum erythrohizon, Codonopsis lanceolata and Aster scaber etc. Medical plants are listed as 48 kinds such as Schizandra chinensis, and A canthopanax sessiliflorus etc. Con Codonopsis Schizandra. It is the first time to report that the populations of Ulmusmacrocarpa, Acanthopanax sessiliflorus, Schizandra chinensis, and Lithospermum erythrorhizon are existed in this area.

This is a study on a landscape planning along the street 1~4, which are gateways to Taegu city and an important scenery. Therefore, emphasis was given to provide a bright, clean and beautiful first impression to visitors and citizens. The area is being developed for education and athletic park. Plans to construct a fountain, pergolas and a pond were set up to complement the active and passive facilities to the existing a athletic facilities in the area. Since D part in the area have alkali soil (pH 8.1), soil amendment is necessary for successful growth of trees and ground covers. When the area is filled or added with new soil for soil amendment, it will be important to use fresh soil which can support plant growth. It will be ideal to use ground covers and shrubs on the existing retaining walls along the street for keeping natural vegetation and slope stability instead of further cutting and reconstruction. It is not reasonable in technical and economic sense to remove rocks of the slope for planting trees. In order to harmonize plant color distribute, tall needle and broad leaf trees in the background as a screen, flowering trees were arranged in groups at front, and annuals were mass-planted along the footway. Natural-form-trees will be planted because they not only serve as a greenbelt but also are more economics in maintenance than formative trees.

사과 Fuji와 국광을 저온(低溫) 상태하(狀態下)에서 저장방법(貯藏方法)을 달리하였을 때, 내부갈변(內部褐變) 및 PPO.의 변화(變化)에 대하여 조사한 결과는 다음과 같다. 1. P.E film저장(貯藏)에 있어서 내부갈변(內部褐變) 정도(程度)는 11월(月)에 수확(收穫)한 Fuji가 10월(月)에 수확(收穫)한 Fuji보다 많았고, 0.09mm 2%구(區)에서 가장 많이 나타났고 0.06mm 2%구(區), 0.06mm 2%구(區)의 순(順)이었으며 를 완전히 제거(除去)한 감압저장(減壓貯藏)에서는 내부갈변(內部褐變)을 볼 수 없었다. 2. P.E film저장중(貯藏中) Fuji의 PPO. activity 및 PPO. specific activity는 11월(月) 수확(收穫)이 10월(月) 수확(收穫)에 비해 높았으며, 0.09mm의 2%구(區)가 0.06mm구(區)보다 높았고, 저장중(貯藏中) 증가(增加)하는 경향이었고, 를 완전히 제거(除去)한 감압저장(減壓貯藏) 동안 Fuji의 PPO. activity는 변화(變化)가 거의 없었으나 PPO. specific activity는 계속 감소(減少)하였다. 3. 국광의 PPO. activity는 Fuji에 비해 상당히 높았고 저장중(貯藏中) PPO. activity 및 specific activity는 저장(貯藏) 초기(初期)와 거의 차이(差異)가 없었다. 4. 이상(以上)으로 미루어 Fuji의 내부갈변(內附褐變)은 저온장해(低溫障害)보다는 장해(障害)로 보이며 품종간(品種間)의 PPO. activity의 정도(程度)와 내부갈변(內附褐變)의 관련(關聯)은 찾을 수 없는 것 같다. 그러나 Fuji의 경우 PPO. activity증대(增大)는 의 영향(影響)을 받은 것으로 보이며 내부갈변(內部褐變)과 어느정도 관련(關聯)이 있는 것 같다.

This report decribes the changes of internal browning and PPO in apples, when the method of storage was changed in the condition of low temperature. Internal browning was found more Fuji harvested in November than Fuji in October. The degree of internal browning was the highest during the storage of 0.09mm 2% and 0.06mm 2%, 0.06mm 20% was followed. In the subatmospheric storage with complete remove of internal browning was not observed. During the storage of P.E. film bag, Fuji havested in November was higher than in October, and Fuji on 0.09mm 2% was higher than in 0.06mm, and Fuji during the storage of P.E. film bag was increased in PPO activity and PPO specific activity. During the subatmospheric storage with complete remove of , PPO activity of Fuji was little changed, but it's PPO specific activity was continuously decreased. PPO activity of Ralls was more higher than PPO activity of Fuji. PPO activity and PPO specific activity during the early stage of storage and the end stage of storage were little changed. Internal browning of Fuji was more effected injury than chilling injury. There is no relation between internal browning and degree of PPO activity was effected by and slightly related with internal browning.

마늘의 저장성(貯藏性) 향상(向上)을 위한 기초자료(基礎資料)를 얻기위해 난지계(暖地系)인 남해재래(南海在來)와 한지계(寒地系)인 달성재래(達成在來)를 각각(各各) 시기별(時期別)로 수확직후(收穫直後) 구(球)의 기부(基部)로부터 줄기길이를 7cm 및 25cm로 남긴 후 열풍건조기(熱風乾燥機)에서 로 1일(日) 12시간(時間)씩 4일간(日間) 건조(乾燥)한 후 중량감소율(重量減少率)을 관행건조(慣行乾燥)와 비교(比較)하고 저장중(貯藏中) 발근(發根), 맹아(萌芽) 및 부패정도(腐敗程度)를 조사(調査)한 결과(結果)는 다음과 같다. 열풍건조(熱風乾燥) 4일후(日後)의 중량감소율(重量減少率)은 남해재래(南海在來)의 7cm 구(球)에서는 관행건조(慣行乾燥) 13일(日)의 효과(效果)가 있었으며 25cm 구(球)에서는 14일(日)의 효과(效果)가 있었다. 달성재래(達成在來)는 7cm 처리(處理)의 4일간(日間) 열풍건조(熱風乾燥) 효과(效果)는 조기수확(早期收穫), 적기수확(適期收穫), 만기수확별(晩期收穫別)로 관행건조일수(慣行乾燥日數)에 각각(各各) 22일(日), 18일(日), 16(日)의 효과(效果)가 있었으며 25cm처리(處理)의 4일간(日間) 열풍건조(熱風乾燥) 효과(效果)는 조기수확(早期收穫), 적기수확(適期收穫), 만기수확별(晩期收穫別)로 각각(各各) 관행건조(慣行乾燥)의 18일(日), 16일(日), 14일(日)의 효과(效果)가 있었다. 저장초기(貯藏初期)의 사경(砂耕)에 의한 발근(發根), 맹아(萌芽)는 열풍건조구(熱風乾燥區)가 관행건조구(慣行乾燥區)에 비하여 지연(遲延)되는 경향(傾向)을 보였다. 저장후기(貯藏後期)의 인편상태(鱗片狀態)는 열풍건조(熱風乾燥)가 관행건조(慣行乾燥)에 비해 식용가능(食用可能)한 인편수(鱗片數)가 많았으며 부패인편수(腐敗鱗片數)는 적었다.

This study was performed to obtain a basic materials for improvement of garlic storage ability. The "Namhae jaerae" of southern strain and the "Dalsung jaerae" of northern strain were harvested by period, their stems were cut at 7cm and 25cm from disk and then placed them in blast drying system 12 hours per day at for 4 days, or in natural state. The decrease rate of bulb weight was compared and rooting, sprouting, the rate of decay was investigated between natural dry(conventional method) and hot-air dry (blast drying system). The results obtained were as follows. In the case of the decrease rate of bulb weight after hot-air dry; 7cm plot" "Namhae jaerae" was similiar to one of 13th day of natural dry and 25cm plot 14th day. In "Dalsung jaerae" 7cm plot of early, common and late harvest was respectively similiar to one of 22nd, 18th and 16th day of natural dry, 25cm plot of early, common and late harvest showed the same decrease rate of bulb weight as that of 18th, 16th and 14th day of natural dry respectively. In the case of rooting and sprouting in sand culture at the early period of storage, hot-air dry showed more prolonged tendency than conventional drying method. In the case of clove state in the latter period of storage, number of eatable cloves was more numerous and number of decayed cloves were less in blast drying system than in conventional method.

저장(貯藏)사과주의 산막(産膜)을 방지(防止)할 목적(目的)으로 사과주 양조장내(釀造場內)의 산막(産膜)이 형성(形成)된 저장(貯藏) 사과주에서 산막효모(産膜酵母) 45종(種)을 분리(分離)하고 그중(中) 가장 뚜렷한 산막생성능(産膜生成能)을 나타내는 균주(菌株) FY-4, FY-5를 선별(選別)하였다. 2균주(菌株) 중(中) FY-5는 분류학적(分類學的) 제성질(諸性質)로 보아 Hansenula beijerinckii 또는 그 근연균(近緣菌)으로 동정(同定)되었다.

Film-formation, which often occurs during storage of apple wine, owing to contamination by film-forming yeasts, results in inferior products. Therefore, for the purpose of preventing this occurrence, we isolated and identified yeast strain. Among the total number of 45 yeast strains which were isolated from contaminated apple wine in winery, the strains FY-4 and FY-5 were found to be useful. The strain FY-5, which greatly formed film on apple wine, was identified as Hansenula beijerinckii or similar strain according to taxonomic characteristics.

소고기의 Chuck부위에 존재하는 색소중 Oxy-와 Metmyoglobin의 농도를 알기 위하여 Hunter-Lab와 분광광도계를 사용하였다. Hunter-Lab에 의한 Oxymyoglobin의 농도는 L, a 그리고 b에서 각각 34.03, 10.40, 그리고 6.50으로 측정되었으며 이들 수치는 CIE Primaries로서는 각각 X, Y 그리고 Z에서 13.33, 11.58 그리고 9.94로 환산되었다. 또 Hunter-Lab에 의한 Metmoyglobin은 L, a 그리고 b에서 39.10, 13.20 그리고 10.13의 수치를 보여줬으며, CIE primaries에서는 17.88, 15.29와 11.40이 X, Y와 Z로 각각 환산되었다. Oxymyoglobin의 Spectrum은 Extinction Coefficient()에서 0.79와 0.83의 수치를 가진 2개의 Peak가 파장 530과 570nm에서 각각 나타났으며 Metmyoglobin의 경우는 Extinction Ceofficient에서 0.58 그리고 파장이 500인 peak을 보이면서 불규칙한 곡선을 보여 주었다.

Oxy - and metmyoglobin concentrations in beef chuck were measured by Hunter-Lab and spectrophotometer. The values of oxymyoglobin by Hunter-Lab got 34.03, 10.40, and 6.50 as average in L, a, and b while the CIE color primaries were 13.33, 11.58, and 9.94 in X, Y, and Z, respectively. The metmyoglobin values recorded 39.10, 13.20, and 10.13 in L, a, and b, of which values were converted to 17.88, 15.29, and 11.40 in X, Y, and Z of the CIE primaries. Absorption spectrum of oxymyoglobin showed 2 peaks which were 0.79 and 0.83 pf extinction coefficient with 530 and 570nm in wavelengh, and that of metmyoglobin appeared on an irregular curve with a summit of 0.58 and 500 in extinction coeffcient (/mg) and wavelength(nm) each.

1. L. acidophilus 성장(成長)을 본 결과 접종후 12시간에 가장 높은 생균수(生菌數)를 측정(測定)하였다. 2. 우유배지(牛乳培地)에서 성장(成長)한 L. acidophilus와 Tomato Juice의 것과 비교할 때 Tomato Juice에서 성장(成長)한 균(菌)이 굵게 보였다. 3. 항생효과(抗生效果)의 분석(分析)은 (1) Well 방법(方法)에서 Acidophilus Tomato Juice의 Acidophilus milk 보다 더 강(强)한 항생대(抗生帶)를 보였다. (2) 디스크 방법(方法)에서 Shigella dysenteriae 병원균(病原菌)에 대하여 가장 강한 항생대(抗生帶)를 보였으며 그외 다른 병원성(病源性)에 대하여서도 모두 항생작용(抗生作用)을 보였다. 4. Sephadex G-50 Gel여과 크로마토그라피의 제 5번째 분류(分溜)가 항생효과(抗生效果)를 가장 크게 나타냈다. 5. UV 흡광 스펙트라에서 약 270nm에서 최고 피크를 보였다.

1. At selected time intervals, viable cell plate counts were run. After the 12 hours incubution the number of viable cells was maximum. 2. The morphology of L. acidophilus in tomato juice was thicker rods than those of in milk media. 3. In the assay of antimicrobial activity; (1) The antimicrobial activity of acidophilus tomato juice showed bigger inhibitory zone than acidophilus milk in the well-cup assay technique. (2) The inhibitory zone against Shigella dysenteriae was the biggest among the other pathogenic organisms in the disc assay. 4. The antimicrobial activity of sephadex G-50 gel filtration chomatography showed in the 5th fraction. 5. The spectra of ultraviolet absorption exhibited around maximum peak at 270nm.

본(本) 연구(硏究)는 한우육용화(韓牛肉用化) 개량(改良)에 있어서 가장 중요한 경제형질(經濟形質)인 체중에 대(對)한 유전적개량량(遺傳的改良量)을 최대(最大)로 증대(增大)시킬 수 있는 적정규모(適正規模)를 산출(産出)하여 한우(韓牛)의 육용능력(肉用能力)을 조기(早期)에 효율적(效率的)으로 개량(改良)할 수 있는 방안(方案)을 제시(提示)하기 위하여 수행되었다. 체중에 대(對)한 유전적개량량(遺傳的改良量)과 세대별(世代別) 체중평균치의 변화(變化)를 추정(推定)하기 위하여 2재(才)이상의 암소잡균(雜菌)은 매세대(每世代) 675,000두(頭)가 유지(維持)되고 또한 암소는 매년(每年) 15%가 도태(淘汰)된다는 등(等) 몇가지 반정(飯定)아래서 다음과 같은 요인(要因)이 고려(考慮)되었다. 1) 암소 집단중(集團中) 인공수정(人工授精)되는 비율(比率); 30, 40, 50, 60, 70% 2) 종모우(種牡牛) 두당, 년간(年間) 정액(精液) 생산(生産) 수량(數量); 5,000, 7,000, 10,000, 15,000, 20,000두분(頭分) 3) 종모우(種牡牛)의 평균체중; 480, 520, 560, 600, 640, 680, 720kg/18개월령(個月齡)이다. 본연구(本硏究)에서 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1) 체중의 유전적개량량(遺傳的改良量)은 이용(利用)되는 종모우(種牡牛)의 체중에 크게 영향(影響)을 받으며 매세대당유전적개량량은 28.66~36.31kg으로 추정(推定)되었다. 2) 유전적개량량(遺傳的改良量)의 80~90%는 종모우선발(種牡牛選拔)에 기인(起因)하는 것으로 나타났다. 3) 종모우(種牡牛)의 평균체중이 매세대(每世代)마다 40kg씩 증가(增加)되고 암소의 인공수정(人工授精)되는 비율(比率)이 50%이상으로 확대(擴大)되어지는 경우 5세대(世代)이후에서 수소 및 암소의 체중평균치는 인공수정집단(人工授精集團)에서는 600kg과 520kg, 그리고 전(全) 모집단(母集團)에 있어서는 560kg과 480kg에 도달될 것으로 추정(推定)되었다.

This study was conducted to find out the most fabourable breeding structure for the maximum genetic gain of live weight, the most important economic traits of Korean native cattle, in order to achieve the improvement goals for meat productivity of the native cattle early and effectively. For estimating genetic gain and population mean changes, the following factors were investigated under the assumation that 675,000 heads of over-two-year old cows were maintained each generation and 15% of the cows were culled every year: 1. The proportion of cow population inseminated by A I bulls; 30, 40, 50, 60, 70% 2. The number of semen doses produced from each A I bull per year; 5,000, 7,000, 10,000, 15,000, 20,000 doses. 3. The average body weight of A I bulls; 480, 520, 600, 640, 680, 720kg/18 months of age. The estimated results are summarized as follows: 1. The genetic gain of live weight is affected greately by the levels of A I bulls' body weight and the genetic gain was estimated 28.66~36.31kg per generation. 2. The proportion of genetic gain from sire selection were estimated 80~90%. 3. When the average body weight of A I bulls increase 40kg per generation and more than 50% of cow population is inseminated by A I bulls, then the phenotypic mean value of live weight of bulls at the age of 18 months and heifers at the age of 2 years are expected to be reached 600kg and 520kg in the A I population; 560kg and 480kg in the whole population, respectively, after 5th generation.

Testosterone propionate가 체성장 사료 섭취량 및 plasma내의 Creatinine과 cholesterol에 미치는 영향을 구명하기 위해 생후 3개월령된 숫 흰쥐 30마리를 대조구 거세구 거세한 후 1 mg, 2 mg. 3 mg, 4 mg으로 각기 호르몬을 투여한 처리구로 나누어 각 5마리씩 배치하여 체중측정과 사료섭취량은 6일간격으로 testosterone주입은 3일 간격으로 처리하고 성장천자(穿刺) 채혈하여 plasma를 분리해 내어 분석했다. 체중의 변화는 거세구가 대조구와 다른 testosterone처리구에 비해 증체가 둔화되었으나 전체적으로 볼 때 각 처리구간에 유의성은 없었다. 사료섭취량은 전체적으로 유의성이 없었으며 사료섭취량의 증감과 증체량을 비교했을 때 부분적으로 평행하는 경향을 보였다. Creatinine의 Plasma내 함량은 처리군간에 고도의 유의성을 나타내었고(P<0.01) 대조구와 비교해 볼 때 거세구는 감소했고 testosterone처리구에서는 2 mg이상의 처리구에서 상승치를 보였으며 특히 4 mg을 처리한 구에서는 큰 증가를 나타내었다. Cholesterol의 Plasma내 함량은 처리구간에 고도의 유의성을 나타내었으며(P<0.01) 대조구와 비교해 볼때 거세구는 증가했고 testosterone처리구에서는 1mg 처리시 동일했으며 그 이상의 hormone 처리에서는 상당히 감소했다.

To investigate the effect of testosterone upon the body weight, feed intake, and creatinine and cholesterol content in the blood plasma, thirty rats which were three months old, were assigned into 6 groups of 5 rats. Those groups included one control group and 5 castrated groups which injected 0 to 4mg of testosterone each. The body weight gain in the control showed relatively higher value than the castrated, showing no significant effect between the groups. Also no significant effect in the feed intake between the groups, due to the amount of testosterone injection, was found. The creatinine content in the plasma showed a highly significant effect between the groups, giving a lower concentration in the castrated group compared with the control while the content in the hormone treated groups increased with increasing amount of testosterone injection. Otherwise the cholesterol concentration in the plasma was inversely proportional to the creatinine in each group.

우백혈청(牛白血病)virus에 대한 혈청항체의 측정(測定)을 위한 간접효소면역법(間接酵素免疫法)(ELISA)의 확립(確立) 및 ELISA법(法)의 감도(感度)를 알기 위하여 한천(寒天) gel 면역확산법(免疫擴散法)(ID)과 비교검토(比較檢討)하였으며 한우(韓牛), 유우(乳牛) 및 육우(肉牛)등 264두(頭)의 혈청(血淸)을 공시(供試)하였다. 혈청(血淸)을 가(加)하지 않은 공(孔)의 흡광치(吸光値)(C)로서 혈청치(血淸値)를 제(除)한 후, 표준(標準)BLV항체흡성혈청치(N)로서 피검혈청치(被檢血淸値)(T)를 나눈치(値)(T-C/N-C)가 1.5이상(以上)인 것을 BLV항체 양성(陽性)으로 하였을 때 gp-ID의 성적(成績)과 98.5%(259/263)가 일치(一致)되었다. gp-ID 향성혈청(陽性血淸) 145예(例)중 144예(例)가 ELISA 양성(陽性)으로 99.6%, gp-ID 음성혈청(陰性血淸) 118예(例)중 115예(例)가 ELISA음성(陰性)으로 97.5%가 일치(一致)되었다. 이상에서와 같이 gp-ID용(用)의 BLV 항원(抗原)을 사용한 ELISA법(法)을 gp-ID와 동등(同等) 또는 더 감도(感度)가 우수한 BLV의 혈청항체측정법 임이 입증되었으며 실용성(實用性)이 충분(充分)하다고 사료(思料)된다.

A microplate enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to bovine leukemia virus(BLV) is described and compared its sensitivity with that of the agar gel immunodiffusion test (ID) with BLV glycoprotein (gp) antigen using 263 sera collected in Korea and Japan. There was 98.5 per cent agreement between ELISA and ID when ELISA value, the value of tested serum(T) was devided with that of standard negative seurm(N) after the value of control was eliminated from T and N (T-C/N-C), of 1.5 or greater was considered positive. One hundred and forty four (99.6%) of 145 sera which were positive by ID were greater than 1.5 by ELISA, and 115 (97.5%) of 118 sera which were negative by ID were less than 1.5 by ELISA. As a result, it suggest that the ELISA test using BLV-gp antigen provides a useful serological tool for the diagnosis of BLV infection.

Aspirin이 위점막(胃粘膜)에 손상(損傷)을 일으키는 기전(機轉)을 해명(解明)하고 침해(侵害)받는 세포(細胞)의 종류(種類) 및 형태(形態)를 구명(究明)하고자 rat에 체중(體重) 1kg당(當) 60mg을 경구(經口) 및 피하(皮下)로 투여(投與)한 후(後) 경시적(經時的)으로 임상소견(臨床所見)을 관찰(觀察)함과 동시(同時)에 위점막세포(胃粘膜細胞)의 형태적변화(形態的變化)를 광학(光學) 및 전자현미경적(電子顯微鏡的)으로 관찰(觀察)한 결과(結果)를 요약(要約)하면 다음과 같다. aspirin의 경구(經口) 및 피하투여(皮下投與)에 의(衣)하여 특별(特別)한 임상소견(臨床所見)이나 위점막(胃粘膜)의 육안적(肉眼的) 및 광학현미경적(光學顯微鏡的) 소견(所見)은 인정(認定)되지 않았으나 미세형태학적(微細形態學的)으로는 투여경로(投與經路)에 관계(關係)없이 명백(明白)한 변화(變化)를 인정(認定)할 수 있었다. 즉(卽) 위점막세포중(胃粘膜細胞中) 가장 심하게 손상(損傷)받는 세포(細胞)는 벽세포(壁細胞)와 주세포(主細胞)로서 벽세포(壁細胞)에서는 SER과 intracellular canaliculi의 증식(增殖)이, 그리고 주세포(主細胞)에서는 RER의 단열(斷裂), 확장(擴張) 및 효소원과립(酵素原顆粒)의 수적감소(數的減少)와 구조적이상(構造的異常)과 같은 가역적(可逆的) 변성변화(變性變化)가 인정(認定)되었으며 점액세포(粘液細胞), 점액경세포(粘液頸細胞), 기저과립세포(基底顆粒細胞)는 비교적(比較的) 경(經)한 변화(變化)를 보였다. 경구투여군(經口投與群)보다는 경미(輕微)하지만 피하주입군(皮下注入群)에서도 경구투여군(經口投與群)에 상응(相應)하는 변화(變化)를 나타낸 점(点)은 aspirin에 의한 위점막(胃粘膜) 손상(損傷)이 aspirin의 직접적영향외(直接的影響外)에 혈중(血中) aspirin 농도(濃度)에 의(依)한 간접적손상(間接的損傷)도 가미(加味)된다는 것을 시사(示唆)하는 형태적소견(形態的所見)이라고 생각(生覺)되었다.

A study was undertaken on rats in order to clarify the gastric mucosal, morphologic response to oral and subcutaneous administration of acetylsalicylic acid(aspirin). Aspirin was administered orally (oral group) and subcutaneously (subcutaneous group) at does rate of 60 mg per kg of body weight per day to 40 normal rats. On the 1st, 3rd, 7th, and 15th day of administration, in addition to clinical observation, 5 rats each from the both groups were sacrificed and examined macroscopically, histologically and electron-microscopically for the morphological changes of gastric mucosal cells with the following results. Although the clinical, macroscopic and histological changes were not significant, marked ultrastructural changes were observed. Parietal and chief cells were affected most severely by the administration of aspirin ; parietal cells showed increase in the number of SER and intracellular canaliculi, where-as in chief cells fragmentation, luminal dilatation, decrease in the number and structural abnormalities of RER were seen. Relatively mild changes were observed in mucous, mucous neck and basal-granulated cells. Although the degree of changes was milder than those of oral group, the similar changes were also observed in the subcutaneous group. From these results, it would be concluded that aspirin injury of gastric mucosa is effected not only by the direct injury to the mucosa but also indirectly by the blood concentration of aspirin.

The study was conducted to indentify systematically current marketing structure and markeiing efficiency of major cash crops such as red peppers and garlics. That various data and information have been used to analyze the problems related, some of them have come from secondary sources, but major parts of real circum stances were primarily obtained from the scheduled field survey. Some of valuable facts and consequences stemmed from the research can be summarized as fellows: First of all, the commercial rates of peppers and garlics produced by sample farms were 87.5 percent and 69 percent, respectively. On the other hand, annual handling volumes per marketing firm of those two crops were on the average estimated into 51,000 kyun(about 600gr) for peppers and 20,000 hundred bulbs of garlic. As seen physical losses over the entire marketing channels of each crop, it was approximately calculated in 14.7 percent of peppers and 21.1 percent of garlic, respectively. Secondarily, it was found that marketing chanells of these crops are very diversified and complex. However, the five-staged chanell is typical: i.e., from producers to local consigners, to local carry-out merchants, to consigner-wholesales, and finally to retailers. Thirdly, it was also estimated that gross marketing margin, marketing cost and profit based on average grade of peppers were 56.1 percent, 22.6 and 33.5 percent, respectively, having 43.8 percent of farmer's share, and those of garlic 38.9, 17.1, 21.8 and 61.1 percent, separately. To the end, it seems that current collective bargaining power of farmers' cooperatives is virtually weak compared to free individual marketing firms in terms of bssiness volumes and quantity handled over the year.

This study attempted to introduce Fei-Ranis's agricultural development theory and discuss its problem for the rural development of less developed world. Fei-Ranis systematized the development process of Western European economy on the ground of dualism. They divided the process into 4 stages by the concept of 'mode of operation'. Paticularly, they consider agrarian mercantilism as take-off stage and its development were achieved by the increase of trade margin and labor productivity. Especially, they thought that only agricultural revolution through the diffusion of internal exchange economy and construction of tree-star system can accomplish favorable transition to industrial capitalism. In order to promote this agricultural development, less developed world must abolish short-run agricultural policy and propel 'learning by the contact' strategy through 'tree-star system' and 'parellel development.' In reality, it was problematic that the contemporary less developed world is trying, in the course of a few decades, to imitate Western European experience with development over the last four centuries. But Fei-Ranis ignored qualitative aspects of agricultural development by tree-star system and also it is criticized that they considered agricultural development process of less developed world follows only that of Western European classical process.