Earticle

The study tested overall engineering properties of the mortar mixed with iron sulfate, which can replace the expensive pigment and represents colors as well. In the experiment with mortar not hardened, in cases of mixture proportion such as 1:1 or 1:3, the flow test data and air content decreased as iron sulfate increased. However, unit volume weight increased. Setting time is delayed as more iron sulfate is added. The compressive strength of the hardened mortar in 1:1 and 1:3 mixture proportion has improved with 9% or less of iron sulfate and decreased with 12% or more. As for drying shrinkage properties compared with plain and irrespective of mixture proportion, it has decreased with 6% or less of iron sulfate adding and increased with excess of 6%. Meanwhile, in regard to chromaticity, a*b* has increased as iron sulfate being added more and the case of mixture proportion of 1:1 has shown higher a*b* than that of 1:3. The value of a*b* in the mixture proportion of 1:1 was higher outdoors than indoors in the initial ageing period. At the later ageing days, a*b* of indoors remained unchanged but that of outdoors went down sharply to the level of indoors at 28 ageing days. a*b* of the mixture proportion of 1:3 showed no difference between indoors and outdoors at the initial ageing period and thereafter, a*b* of indoors remained stable while a*b* of outdoors went down and even became less than that of indoors at 28 ageing days.

비료중에 함유된 주성분 및 미량원소 함량이 ICP-OES로 측정되었고 분해는 염산 또는 회화(ashing) 로 이루어졌다. P 함량이 높은데도 불구하고 기존의 AAS로 측정할 때 보다 ICP-OES 측정이 보다 더 신뢰할 만 하였다. 고체시료, 특히 오스트리아 동부지역 토양시료들은 왕수로 분해하고 또한 대자율을 측정하였다. 염 기성슬래그와 염기성 slag potash에서 대자율이 높았으며 다른 비료들은 대자율이 훨씬 더 낮았다. 따라서 대 자율 측정은 unknown samples의 이러한 혼합성분들의 탐지에 적절할 것이다.

a set of various fertilizer samples, main and trace elements were determined with ICP-OES after dissolution with hydrochloric acid, or ashing with subsequent dissolution, respectively. In spite of high P contents, ICP-OES gave reliable results when compared to conventional AAS determinations. With respect to previous investigations, trends in Cd, Cr, Zn and Ni contents of fertilizers were observed. The solid samples were also submitted to magnetic susceptibility measurements. Similarly, a set of soil samples from a remote plain area in the East of Austria was investigated for aqua regia leachables and magnetic measurements. High magnetic susceptibility was found only in basic slag and basic slag potash. Other fertilizers were much lower in magnetic susceptibility than the soils investigated. Thus, fertilization would not interfere with magnetometric profiling for the detection of environmental contaminations in the field.

Hydroxyproline (HYP) is a post-translational product of proline hydroxylation catalyzed by an enzyme prolyl 4-hydroxylase (P4H) which plays a crucial role in the synthesis of all collagens. Considering the role of collagen and its significance in many clinically important diseases such as liver fibrosis, a great deal of attention has been directed toward the development of an assay at cell-based system. The reason is that cell-based assay system is more efficient than enzyme-based in vitro system and takes much less time than in vivo system. Several assay procedures developed for P4H are laborious, time-consuming and not feasible for the massive-screening. Here, we report the cell-based assay method of prolyl 4-hydroxylase in immortalized rat hepatic stellate HSC-T6 cells. To optimize the cell culture condition to assay for HYP content, various concentrations of reagents were treated for different times in HSC-T6 cells. Our data showed that the treatment with ascorbate in a hypoxic condition for 24 h resulted in the maximal increase of HYP by 1.8 fold. Alternatively, cobalt chloride ($5\;{\mu}M$) and ascorbate ($50\;{\mu}M$) in normoxic states exhibited similar effect on the production of HYP as in a hypoxic condition. Therefore, cobalt chloride can be substituted for a hypoxic condition when an anaerobic chamber is not available. Rosiglitazone and HOE077, known as inhibitors of collagen, synthesis decreased P4H enzyme activity by 32.3% and 15%, respectively, which coincided with previous reports from liver tissues. The level of the smooth muscle ${\alpha}$-actin, a marker of activated stellate cells, was significantly increased under hypoxia, suggesting that our experimental condition could work for screening the anti-fibrotic compounds. The assay procedure took only 3 days after treatment with agents, while assays from the primary stellate cells or liver tissues have taken several weeks. Considering the time and expenses, this assay method could be useful to screen the compounds for the inhibitor of prolyl 4-hydroxylase.

This study compares the performance of various convolutional neural network (CNN) models for building an automated deep learning-based letter screening system targeting letters received by inmates in correctional institutions. The models evaluated include well-known architectures such as MobileNet, ResNet, and Inception, as well as recently proposed lightweight models such as ResMobileNet and IGSe, along with GroupConv and SE. Each model was trained on image data containing the Korean word for "knife" ("칼") to assess performance in terms of accuracy, processing time, and model compactness. A total of 1,197 letter image samples were used in the experiment, including 1,140 images with normal words and 57 images containing the target word. The experimental results showed that the MobileNet model had the shortest processing time, making it suitable for real-time applications, while the IGSe model achieved the highest accuracy, demonstrating optimal performance for letter screening tasks. This study suggests that deep learning-based screening techniques can be effectively applied to enhance digital security in the management of inmate correspondence within correctional institutions.

Many basidiomycetes, especially mushroom species, thrive on saccharides that they obtain from the decomposition of cellulose, hemi-cellulose and or lignin. Cellulose is degraded by specific enzymes called cellulases. Cellulases play an important role in the biosphere by recycling cellulose, the most abundant carbohydrate produced by plants. Also cellulases have many potential biotechnologic and industrial applications. Detection of cellulose degrading activity is commonly performed on carboxymethylcellulose CMC medium in combination with a stain that reveals the degraded CMC. In order to efficiently screen the F. velutipes knockout library, obtained through Agrobacterium-mediated transformation (ATMT), for those important enzymes we compared two different staining methods i.e. Congo Red and Gram’s Iodine (as reported in R. C. Kasana et al 2008). The latter stain showed strongly enhanced detection in time and intensity, facilitating mass screening of our mutant database. Using Gram’s iodine and square petri dishes containing up to 9 colonies at a time we can now rapidly screen multiple mutants in a short period. We are going to find the genes. Inactivated genes of mutants with altered cellulase degradation activity will be identified and cloned for further analysis.

Background: The number of synthesized chemicals has rapidly increased over the past decade. For many chemicals, there is a lack of information on toxicity. With the current movement toward reducing animal testing, the use of toxicity big data and deep learning could be a promising tool to screen potential toxicants. Objectives: This study identified potential chemicals related to reproductive and estrogen receptor (ER)-mediated toxicities for 1135 cleaning products and 886 laundry products. Methods: We listed chemicals contained in cleaning and laundry products from a publicly available database. Then, chemicals that potentially exhibited reproductive and ER-mediated toxicities were identified using the European Union Classification, Labeling and Packaging classification and ToxCast database, respectively. For chemicals absent from the ToxCast database, ER activity was predicted using deep learning models. Results: Among the 783 listed chemicals, there were 53 with potential reproductive toxicity and 310 with potential ER-mediated toxicity. Among the 473 chemicals not tested with ToxCast assays, deep learning models indicated that 42 chemicals exhibited ER-mediated toxicity. A total of 13 chemicals were identified as causing reproductive toxicity by reacting with the ER. Conclusions: We demonstrated a screening method to identify potential chemicals related to reproductive and ER-mediated toxicities utilizing chemical toxicity big data and deep learning. Integrating toxicity data from in vivo, in vitro, and deep learning models may contribute to screening chemicals in consumer products.

The white button mushroom, Agaricus bisporus, is commercially the fifth most important edible mushroom, accounting for the production of 9,732 tons of mushrooms in Korea in 2015. The genus Agaricus has been known for its potential to degrade lignocellulosic materials. Chemical analyses carried out during the cultivation of A. bisporus indicated that the cellulose, hemicellulose, and lignin fractions were changed preferentially for both vegetative growth and sexual reproduction. We screened A. bisporus strains for effective biodegradation through extracellular enzyme activity using cellulase, xylanase, and ligninolytic enzymes. The enzyme biodegradations were conducted as follows: mycelia of collected strains were incubated in 0.5% CMC-MMP (malt-mops-peptone), 0.5 Xylan-MMP, and 0.5% lignin-MMP media for 14 days. Incubated mycelia were stained with 0.2% trypan blue. Eighteen strains were divided into 8 groups based on different extracellular enzyme activity in MMP media. These strains were then incubated in sterilized compost and compost media for 20 days to identify correlations between mycelial growth in compost media and extracellular enzyme activity. In this study, the coefficient of determination was the highest between mycelial growth in compost media and ligninolytic enzyme activity. It is suggested that comparison with ligninolytic enzyme activity of the tested strains is a simple method of screening for rapid mycelial growth in compost to select good mother strains for the breeding of A. bisporus.

Trichoderma disease of oyster mushroom has not been effectively detected in the field for testing its resistance against the disease with its varieties. In this study, we investigated the methods to detect its resistance in the laboratory by using media, which enables us to understand the relevant characteristics (e.g., lysis, toxin enzyme, mycelial growth rate). In coculturing with strains of Trichoderma and oyster mushroom, it is possible to observe the difference in the resistance of oyster mushroom against Trichoderma with the phenomena of barrage reaction, overgrowth and lysis. We also observed the inhibition of mycelial growth of oyster mushroom using the dilution method with 48-well plate, but could not observed the inhibition of mycelial growth using the filter paper method of cultural supernatant. In simultaneously culturing both Trichoderma and oyster mushroom, it was possible to detect the inhibition of the mycelial growth of oyster mushroom, but Trichoderma mycelium did not overgrow against oyster mushroom. We found that the pathogenicity was efficient in using solid medium with the phenomena of overgrowth and lysis by inoculating Trichoderma on top of mycelia of oyster mushroom. In conclusion, the methods (e.g., coculture method, dilution method with 48 -well plate, post-inoculation method) are recommended to detect the resistance of oyster mushroom against Trichoderma disease.

Although atopic dermatitis (AD) is one of the most common skin disease, specially in children, it is poorly characterized of pathogenesis and is having difficulty for the development of therapeutic agent. Therefore we reviewed previous studies for atopic dermatitis biomarkers and screening method for the measurement of clinical severity, and suggest a development direction of atopic dermatitis therapeutic agent. Biomarkers for AD was investigated in terms of immune and genomic biomarkers, and main biomarkers of AD were focused on immune biomarkers based on the pathogenesis mechanism. The available immune biomarkers of AD were IgE, IL-4, IL-5, IL-6, IL10, IL-12 and IL18; the genomic biomarkers were chromosome 5q31-33, chromosome 16p12 and HLA-DRB1*15 gene. The screening method of AD has not been established, but SCORAD index was only applicable. SCORAD index measures three parameters, such as extent, intensity, subjective symptom, and is the most accurate and objective method to measure AD severity, and to identify the efficacy of therapeutic agent. Finally, this review article suggests good biomarkers and screening method for AD, and those would contribute to understand the pathogenesis of AD and to develop therapeutic agents.

본 논문에서는 MIL-HDBK-344A 기반의 ESS(environmental stress screening) 시험 에 대해 소개하고, 유도탄 내부 케이블의 ESS 시험에 대한 목적과 규격 설정 및 시험 방안에 대하여 확인하였다. ESS 시험구성은 온도 순환과 랜덤 진동의 환경 자극으로 이루어져 있으며, 핸드북의 절차를 기반으로 유도탄 내부 케이블의 ESS 시험 규격 설정과 시험 구성을 제시하고, 진동 시험을 위한 유도탄 내부 케이블의 ESS 시험을 위한 치구를 제작하였다. 스크린 강도(SS; screening strength)를 통해 결정되는 결함 감지 확률은 일회성으로 사용되는 유도탄의 신뢰성에 부합되도록 선정하였고, 또한 ESS 시험 중 유도탄 내부 케이블의 검사방법과 고려해야 할 점에 대하여 소개하였다.

In this paper, the ESS test based on the MIL-HDBK-344A is introduced, and the purpose of the ESS test of the guided missile internal cable, and the specification and test method are confirmed. The ESS test configuration consists of environmental stimulation of temperature circulation and random vibration. Based on the procedure in the handbook, the ESS test specification setting and test configuration of the guided missile internal cable are presented, and the jig for the ESS test of the guided missile internal cable for the vibration test was produced. The probability of detecting a defect determined through the screening strength(SS) was set to match the reliability of a one-time guided missile, and also introduced the inspection method and considerations for the cable inside the guided missile during the ESS test.

In coculturing with strains of Trichoderma and oyster mushroom, we could detect the difference in the resistance of oyster mushroom against Trichoderma with the phenomena of barrage reaction, overgrowth and lysis. We selected the isolates ASI 2183, ASI2504 and ASI 2477 as varieties that showed the resistance. The isolates ASI 2240, ASI 2479 and ASI 2181 were the best in their resistance against Trichoderma in the method using culture filtrate. In common, the isolates ASI 2479 and ASI 2240 were selected in both methods. In post-inoculation method, the isolates ASI 2479, ASI 2333 and ASI 2181 were selected and ASI 2302 was susceptible. For the same isolate of Trichoderma, the resistance varied depending on the isolates of oyster mushroom used in the experiments. Because we could detect the interactions between Trichoderma and oyster mushroom, it is possible to detect the level of the resistance that differs in the varieties. However, there were the cases of detecting the level of the resistance in repetitions with the same isolate, which may be caused by the vitality of isolates of Trichoderma and oyster mushroom. It is efficient to test the resistance with the resistant isolate of Pleurotus salmoneostramine and the susceptible isolate of ASI 2302.

Diplomatic pouches are protected by the 1961 UN Vienna Convention on Diplomatic Relations. In Article 27, Paragraph 3 of the Convention, it is stipulated that “diplomatic pouches cannot be opened or detained.” Since it is impossible to search or customs inspection, it is virtually impossible to check the contents. The Ministry of Foreign Affairs and Trade operates diplomatic pouches as a means of transporting official documents and common items between the headquarters and diplomatic missions abroad. You can see that a bag or pouch for sending documents is expressed as a diplomatic bag. In the case of Diplomatic Pouch certified by the Minister of Land, Infrastructure and Transport, there is no problem because they are exempt from security screening. In order to prevent the occurrence of security screening complaints at security checkpoints, it is necessary to revise the regulations on the special security screening method for Diplomatic Pouch. For Diplomatic Pouch, it is institutionalized to apply to the head of the regional aviation office for a certificate from the relevant national mission proving that there are no dangerous objects such as weapons or explosives that can be used to Act of Unlawful Interference. Diplomatic Pouch The occurrence of complaints should be minimized.

FM screening has been praised for its apparent resolution advantage over conventional AM screening. FM screening is also known for its criticalness of film output and difficulties in the proofing stage because of the microdot formation. However, FM screening is not a well understood process from press performance point of view. This study was carried out for the purposed of introduction the availability of hifi-offset printing to domestic printing industries and evaluating the print qualities between 300pli AM screen and FM screen with the equivalent sized microdots to 4-5% dot area of the 300lpi AM screen, together with comparing with the 175lpi AM screen representing a conventional printing. Solid density, dot gain, color gamut and some other quality properties were measured and discussed.

복분자 와인의 제조를 위하여 복분자 과실 및 엑기스로부터 야생효모 Saccharomyces cerevisiae BA29를 분리 및 동정하였으며, 생화학적 특성 및 biogenic amine 생성 여부, 배양학적 특성 및 알코올 발효능과 알코올, 당, 아황산 저항성을 조사하였다. 또한 S. cerevisiae BA29의 산업적 공정 적용을 위한 균체량 증가를 위하여 통계학적 방법인 반응표면분석법을 사용하여 배양 배지 조성의 최적화를 수행하였다. 실험계획법은 중심합성계획을 사용하여 모델을 설정하였고, 산업적 공정 적용 시 비용 대비 효율성이 높은 molasses를 대체 탄소원으로 사용하여 실험을 수행하였다. 통계프로그램을 이용하여 분석한 결과 최대 균체 성장을 위한 배지 조성으로는 molasses 200 g/L, peptone 30 g/L, yeast extract 40 g/L로 예측되었으며, 이때의 최대 균체량은 20.6565 g/L로 예측되었다. 모델의 검증실험 결과 기본 배양 배지와 비교하였을 때 6.39 g/L에서 $20.9167{\pm}0.7925g/L$로 약 3.27배 증가하였다. 최종적으로 S. cerevisiae BA29를 사용하여 복분자 와인을 제조한 결과 20.33%의 알코올 생성능을 나타냈다. 이로써 복분자 과실로부터 분리한 효모 S. cerevisiae BA29를 이용한 우수한 복분자 와인 제조의 가능성을 확인하였다.

Rubus coreanus is known as Korean black raspberry, native to Korea, Japan, and China. Preliminary studies evaluating their potential for cancer treatment in mammalian test systems are ongoing. In recent years, interest has been renewed due to their high levels of anthocyanins. Anthocyanins in black raspberry are important due to their potential health benefits as dietary antioxidant, anti-inflammatory compound, and as a chemopreventive agent. In the present study, Saccharomyces cerevisiae BA29 was isolated from black raspberry fruit and fruit juice as a biogenic amine non-producing strain for manufacturing of black raspberry wine, after which we investigated its characteristics: biogenic amine-producing ability, cell growth ability, alcohol-fermentation ability, and resistance to alcohol, glucose, and sulfur dioxide. Based on preliminary experiments, we optimized culture medium compositions for improving dried cell weight of S. cerevisiae BA29 by response surface methodology (RSM) as a statistical method. Design for RSM used a central composite design, and molasses with the industrial applicability was used as a carbon source. Through statistical analysis, we obtained optimum values as follows: molasses 200 g/L, peptone 30 g/L, and yeast extract 40 g/L. For the model verification, we confirmed about 3-fold improvement of dried cell weight from 6.39 to 20.9167 g/L compared to basal yeast peptone dextrose medium. Finally, we manufactured black raspberry wine using S. cerevisiae BA29 and produced alcohol of 20.33%. In conclusion, S. cerevisiae isolated from black raspberry fruit and juices has a great potential in the fermentation of black raspberry wine.