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Developing an efficient screening method for detection of F. velutipes mutants with altered cellulose degradation activity.

첫 페이지 보기
  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    버섯 바로가기
  • 통권
    제15권 1호 통권 23호 (2011.05)바로가기
  • 페이지
    pp.46-46
  • 저자
    Jasun Koo, Arend F. van Peer, Sung-i Woo, Pyung-Gyun Shin, Kab-Yeul Jang, Young-Bok Yoo, Won-Sik Kong
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A193596

원문정보

초록

영어
Many basidiomycetes, especially mushroom species, thrive on saccharides that they
obtain from the decomposition of cellulose, hemi-cellulose and or lignin. Cellulose is
degraded by specific enzymes called cellulases. Cellulases play an important role in the biosphere by recycling cellulose, the most abundant carbohydrate produced by plants.
Also cellulases have many potential biotechnologic and industrial applications. Detection of cellulose degrading activity is commonly performed on carboxymethylcellulose CMC medium in combination with a stain that reveals the degraded CMC. In order to efficiently screen the F. velutipes knockout library, obtained through Agrobacterium-mediated transformation (ATMT), for those important enzymes we compared two different staining methods i.e. Congo Red and Gram’s Iodine (as reported in R. C. Kasana et al 2008).
The latter stain showed strongly enhanced detection in time and intensity, facilitating mass screening of our mutant database. Using Gram’s iodine and square petri dishes containing up to 9 colonies at a time we can now rapidly screen multiple mutants in a short period.
We are going to find the genes. Inactivated genes of mutants with altered cellulase degradation activity will be identified and cloned for further analysis.

저자

  • Jasun Koo [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-707. Republic of Korea. ]
  • Arend F. van Peer [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-707. Republic of Korea. ]
  • Sung-i Woo [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-707. Republic of Korea. ]
  • Pyung-Gyun Shin [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-707. Republic of Korea. ]
  • Kab-Yeul Jang [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-707. Republic of Korea. ]
  • Young-Bok Yoo [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-707. Republic of Korea. ]
  • Won-Sik Kong [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-707. Republic of Korea. ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    버섯 [Mushroom]
  • 간기
    반년간
  • 수록기간
    1997~2025
  • 십진분류
    KDC 525 DDC 635

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