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Hypochlorous acid (HOCl) water was initially developed in Japan in 1987 and its antimicrobial property was reported in 1992. HOCl water is used globally to improve public health and recently started to use in agriculture. It is generated through a process called electrolysis. General property of HOCl water is low in pH, a high oxidation-reduction potential, high available chlorine concentration and high dissolved oxygen. The property of HOCl water depends on the parameters such as concentration of the ingredient NaCl and HCl, voltage, and electrolyte flow rate used in the electrolysis process. Recently, mixing type of HOCl generation without electrolysis, hydrogen-mineral HOCl (HM-HOCl), was developed and started to be used in agriculture due to several advantages such as easy production of high-concentration and high-capacity HOCl, long-lasting HOCl concentration, low level of harmful material and easy handling. Several experiments revealed that HM-HOCl is more available than electrolyzed HOCl to apply to various fields such as organic farming without pesticide, aquaculture and fish farming, and livestock breeding. It is very useful for decreasing stink in cattle shed, preventing viral disease of fishes, and decreasing mortality rate and increasing growth rate of broiler chickens by drinking the high concentration HOCl water (free active chlorine 50 ppm). The results of HM-HOCl usage was satisfied by farmers and governmental institutions of Korea. Therefore, we would like to introduce HM-HOCl, a new concept of disinfectant, and expand its usage in sustainable development in agriculture and rural environment.

Hypochlorous acid has very ideal condition such as powerful and broad sterilizing effect, easy accessibility, low price and high safety as a disinfectant. It can be applied by various ways, but the effect may vary depending on the method, so it is necessary to establish use standard for the maximized effect. In this study, we investigated the sterilizing effect of hypochlorous acid indoor environments contaminated with Staphylococcus aureus using fogging method. Hypochlorous acid water was classified two: Huureka-E generated by electrolysis [pH 5.4, oxidation-reduction potential (ORP) 530, active chlorine concentration (ACC) 51], and Huureka-M produced by a mixing and dilution method (pH 5.5, ORP 913, ACC 53). Blood agar plate were put on the table 1 m high in an enclosed room, then hypochlorous acid was fogged up to 80% humidity, and S. aureus-diluted solution was sprayed three times on the air. The plate was moved for bacterial culture 0 min 30 min, 60 min, and 90 min after spraying bacteria. As a result of the bacterial culture, the growth inhibitory rate of Huureka-M was 92.2 ± 0.8%, 96.0 ± 0.6% and 97.4 ± 0.1% time-dependently (p < 0.001 vs. 0 minm, respectively), and that of Huureka-E was 66.1 ± 1.3%, 76.4 ± 2.4% and 98.2 ± 0.6% (p < 0.001 vs. 0 min, respectively). In confluence, the fogging of both electrolysis-type and mixing-type hypochlorous acid water showed highly satisfactory sterilizing power as a disinfectant. Since spraying or fogging is a highly effective application method for a large space, hypochlorous acid can be recommended to be utilized in the hospital, agriculture sector, livestock industry, public facilities, etc. Further study will be necessary to be standardized the sterilizing protocol according to the application method and ACC.

About two billion people in the world drink alcoholic products and among them, more than 76 million are suffering from serious alcoholic disorder including hangover. Hanover can be caused by the by-products which are produced in the process of alcohol metabolism such as acetaldehyde and acetate in human body. There are various type of anti-hangover-related commercial products which can help to somewhat reduce hangover symptoms. In this study, we investigated the effects of Quechung which is a granule-type of anti-hangover compounds containing functional substances such as HK mycelial culture of Lentinus edodes, Hovenia dulcis fruit extract, Lactobicillus fermentum MG590 and so on. The subjects were 20 healthy men and women, and washout period for the crossover design was 7 days. Participants were randomly divided into two groups: the control group (alcohol + purified water) and the experimental group (alcohol + Quechung). The experimental group was first supplied with the anti-hangover compound before and after 30 min of 20% alcohol intake (7.8 g/kg body weight). Blood pressure, temperature, heart rate, and alcohol concentration in breath and blood were measured one time before alcohol intake and also 3 times after Quechung intake at an interval of 1 h. Biochemical parameters such as albumin, electrolytes, lipid profile, liver function markers, alcohol level in breath and blood, acetaldehyde concentration were analyzed. Consequently, breath alcolol concentration was lower in the experimental group than control group after 1 h, especially showed statistical difference in female. Blood alcohol concentration before and after treatment also showed significant decrease in experimental group, whereas blood acetaldehyde showed significant increase in control group. Especially female participants showed higher effect than male. In the reslut of AST, experimental group showed little difference before and after treatment but control group showed significant increase, in addition female much higher effect than male. Taken together, this anti-hangover compound, Quechung, clinically showed the hangover-relieving effect on the acute alcohol-induced hangover symptom based on the positive results of alcohol concentration, acetaldehyde concentration, and level of liver function markers etc. in addition, Quechung also can influence on the protection of liver and blood vessel. This clinical study has a significance in that it provides scientific evidence to systemically establish database for the effectiveness and efficiency of the commercial anti-hangover products, and to help consummer’s easy choice and their better health.

The major purpose of tour is rest and we are facing corona virus threatening, so people looking for healing tour to solve stress driven problems and get health. Two major area of healing tour is beauty and health and hydrogen related directly to support both effect. Healthy skin and beauty are considered as a cornerstone of general well-being in human. Skin as a largest body organ plays an important role in body protection against environmental, physical, chemical and biological factors. These factors include agents that may act as oxidation reactions that produce ROS/RNS, and other oxidants in skin cells. An increased amount of the oxidants, exceeding the antioxidant defense system capacity is called oxidative stress, leading to chronic inflammation, which, in turn, can cause collagen fragmentation and disorganization of collagen fibers and skin cell functions, and thus contribute to skin diseases including psoriasis, atopic dermatitis and ageing. In this review, we report here a summary of present state of knowledge related to role of oxidative stress in pathogenesis of dermatological disorder and its impact on beauty and day to day lives of patient and discuss how molecular hydrogen showed therapeutic effect against skin diseases through effects on oxidative stress. A finding from this summary indicates that molecular hydrogen might be effective treatment modalities for prevention and treatment of skin diseases.

As the aging population is rapidly increasing in the world, geriatric diseases such as dementia, hypertension, diabetes, cerebrovascular diseases, and degenerative diseases are emerging as social problems. Along with this, the prevalence of dementia is also rising rapidly. There are quite variety of diseases that can cause dementia, among which reactive oxygen species (ROS) receive attention as an etiological factor. ROS is associated with about 90% of modern diseases. It is known to be the leading cause of aging, and is closely related with cancer, diabetes, dementia, chronic diseases, etc. In 2007, Ota Shigeo research team reported that hydrogen water could selectively react with ROS such as OH-, which led to the discovery of the anti-oxidant effect of molecular hydrogen. Therapeutic effect of H2 was reported through in vivo and clinical studies including improvement of metabolic syndrome, blood-brain barrier breakdown, Alzheimer's disease, Parkinson's disease, cerebral infarction, neurodegeneration, cognitive ability, dementia, and bedsores. In addition, antioxidant effect on the blood, sterilizing effect on oral bacteria, anti-fungal effect, anti-algae effect and improvement of skin troubles were also reported through hydrogen treatment. In conclusion, several studies have shown that hydrogen has excellent antioxidant effects. Moreover, studies have also shown its safety to the human body and environment. It is essential to verify the effect of H2 and to provide clinical application especially to the aging population suffering from dementia or various geriatric diseases in Korea, which is about to enter the super-aging society.

There are countless nutritional supplements, medical technologies and medicines that have been remarkably developed day to day, however, human diseases also have been diversified along with these innovative health technologies. According to statistics of the National Health Insurance Service, patients suffering from chronic diseases are on an increasing trend due to the soaring population of senior citizens. Recently, it is accepted that an excessive free radicals such as reactive oxygen species (ROS) is closely related to aging and a cause of various diseases such as cancer, dementia, diabetes, etc. Free radicals such as •O−2, H2O2, and •OH are formed as a natural byproduct of the normal metabolism of oxygen (O2), and have important roles in cell signaling, homeostasis and antibiosis. However, excessive production of ROS attack and destroy cells, organs and tissues, which causes oxidative damage. It is known that around 90% of diseases is caused by oxidative stress. As a strong anti-oxidant which reduces levels of toxic ROS, molecular hydrogen currently receives attention in the medical field. Hydrogen acts as an anti-oxidant in the body, including infarcted brain and blocked blood vessels. Hydrogen is an essential constituent of human body, and is one of the components of fats, proteins and carbohydrates. Hydrogen is discharged as water by being combined with ROS in the body. It increases immunity, keeps the cells healthy, prevents various diseases, increases energy, and slows down the aging process. Recently, it was reported that hydrogen therapy clinically alleviated the symptoms of COVID-19 patients. Thus, hydrogen medicine is expected to be an alternative for the solution of aging and intractable diseases.

Application of hydrogen has numerous positive effects such as selectively scavenging effect on free radicals, which act as an causal factor of many oxidative stress-related diseases. With this, disease-prevention and anti-aging effect through hydrogen intervention have been explored and proven. In addition to health care, hydrogen also shows excellent effect on skin beauty. In 2010, we opened Hydrogen Healing Center (HEALINGTOPIA Co.) in Sunae-dong, Bundang, Seongnam, Gyeonggi-do, and gave our services to more than 3,500 customers till date. The center was opened with the goal to promote and experience hydrogen-related healthcare products (BIOSUSONARA) through introducing HEALINGTOPIA Customer Care Programs as below: (1) Far-infrared Resonance Magnetic Hydrogen Spa (Heat, Magnetic field and Hydrogen treatment) (2) Hydrogen Respirator (Hydrogen gas inhalation) (3) Hydrogen美tox (directly spraying of hydrogen and hydrogen water on the skin) (4) Drinking hydrogen water (5) Hydrogen foot bath The management category of customers and the improved result are as shown in Table 1. The annual sales growth rate of the hydrogen-related products (BIOSUSONARA) are steadily increasing because of the increase of the customers who want to use the hydrogen-related products at home in their daily life. The purchase of the customers is based on the experience of hydrogen effect at the HEALINGTOPIA center. Also, there is continuous promotion of using hydrogen-related products because of the positive results from the satisfied customers, which act has an advertising impact to other people.

Care farming is the use of farming practices for the stated purpose of providing or promoting healing, mental health, social, or educational care services. Hydrogen disinfectant can be used at care farming to raise plant and animal and can hydrogen be used to improve health by installing several devices which is hydrogen producing at home. And the more, mixing type of HOCl generation without electrolysis, hydrogen mineral HOCl (HM-HOCl), was developed recently and started to be used in agriculture due to several advantages such as easy production of high-concentration and high-capacity HOCl, long-lasting HOCl concentration, low level of harmful material and easy handling. Several experiments revealed that HM-HOCl is more available than electrolyzed HOCl to apply to various fields such as organic farming without pesticide, aquaculture and fish farming, and livestock breeding. It is very useful for decreasing stink in cattle shed, preventing viral disease of fishes, and decreasing mortality rate and increasing growth rate of broiler chickens by drinking the high concentration HOCl (50 ppm). The results of HM-HOCl usage is satisfied by Korean farmers and governmental institutions. Therefore, we would like to introduce MH-HOCl, a new concept of disinfectant, and expand its usage in sustainable development in agriculture and rural environment. HM-HOCl would be one of important factor to make success hydrogen care farming in Korea. We are faced corona virus threatening and care farming might be one of solution to live safe from it.

Health is the most important aspect of human life. Recently, there has been a rapid increase in consumer interest in the health-enhancing roles of specific foods and water as it posses’ physiologically active components, so-called as functional foods and functional water. Hydrogen-rich and electrolyzed alkaline waters have been reported as types of functional waters that may ameliorate various oxidative stress–related diseases including various dermatological problems as it has potent anti-oxidant effects. In recent years, molecular hydrogen (H2) has attracted lots of attention in biomedical community such as food and cosmetic industries because of its abilities to modulate signal transduction, protein phosphorylation, and gene expression that enable its anti-inflammatory, anti-allergy, and anti-apoptotic activities. Due to its easy delivery in human body through inhaling H2 gas, drinking H2-dissolved water (H2-water), injecting H2-saline, taking an H2 water bath and H2-spray; popularity of hydrogen business is increasing in many countries including Japan, China and Korea especially in food and cosmetic industries. In Japan, China and Korea, hydrogen-containing beverages and cosmetic items are commercially available in the market. Besides this, new concept of hydrogen-tourism is getting famous in these nations which focuses on the overall holistic approach of human health through introducing various products related with H2 in forms of beverages (coffee, water) and cosmetic products for enhancing beauty and hydrogen spa for relaxation of body and mind and gaining attraction of people.

Health and vibrant life are required by everyone around the globe. To improve quality of life, maintaining a healthy state, and to prevent various diseases, and to evaluate the effects of potentially improve quality of life increasing factors are important. In recent years, oxidative stress has been postulated to be an important factor in the pathogenesis and development of lifestyle-related diseases, cancer, and the aging process. Despite the clinical importance of oxidative damage due to oxidative stress, antioxidants have been of limited therapeutic success. Of these, molecular hydrogen (H2) is known as a “novel” antioxidant in preventive and therapeutic applications. H2 shows not only effects against oxidative stress but also various anti-inflammatory and anti-allergic effects. Thus, H2 is nowadays used in daily life as well as and in medical field in both developed and developing countries such as Japan, USA, and China. Unfortunately, in Vietnam, one of the developing countries in the world has a poor health system and a high death rate due to non-communicable diseases like cancer, cardiovascular, and diabetes. Growing evidence has shown that hydrogen gas can either alleviate the side effects caused by conventional chemotherapeutics, or suppress the growth of cancer cells and xenograft tumor, suggesting its broad potent application in clinical therapy. Besides that studies have reported that H2 also has effects of on diseases such as diabetes, cerebral infarction, and Alzheimer’s disease. However in Vietnam, currently there are only a few research related to H2 has conducted that mainly focus on the application in the environment, energy industry. Therefore, H2 in Vietnam in medical field needs to be exploited in the future as it is completely new area.

Various neurodegenerative diseases including dementia and cognitive dysfunction pathogenesis are caused due to oxidative stress. Several studies have proved that molecular hydrogen (H2) acts as a therapeutic and preventive anti-oxidant, however, H2 effect on dementia is poorly documented. In the present study, we investigated the effects of H2 inhalation on trimethyltin (TMT)-induced memory dysfunction in the C57BL/6 mice. For this, mice were intraperitoneally injected a single dosage of TMT 2.6 mg/kg body weight. The subjects were randomly divided into the following groups: normal control without TMT induction, TMT only as a negative control (TMT injection + saline administration), positive control group [TMT injection + lithium chloride (50 mg/kg body weight) injection], H2-2wk group [TMT injection + H2 gas inhalation (once a day for 30 min for 2 weeks) and H2-4wk group [TMT injection + H2 gas inhalation (once a day for 30 min for 4 weeks). As a result, TMT-exposed mice showed symptoms such as tremor, seizure and aggressive behavior. Meanwhile, H2-treated groups after TMT treatment showed significantly increased spatial cognition abilities in the Y-maze test. Likewise, the level of the oxidative stress markers such as ROS, NO and MDA were found to be decreased in H2-2wk and H2-4wk groups as compared to the TMT only group in both serum and brain lysate, coherently, antioxidant enzymatic activities were increased in H2-2wk and H2-4wk groups compared to TMT only group. Moreover, H2 showed prevention effect of neuronal apoptosis through the suppression of apoptotic signaling molecules such as BAX, and cleaved caspase-7. Overall, H2 inhalation exhibited memory enhancing activity and decreased the oxidative stress markers and suppressed apoptotic signaling molecules on TMT-inducd mice. Therefore, H2 might be one of candidates for repairing dementia and neuro-degenerative diseases which shows symptoms such as dysfunction of learning, memory and cognition. However further studies are needed to clarify this notion.

Hypochlorous acid water can promote pruritus as an adverse effect under certain conditions, which should be taken into account to ensure safety. In recent days, Mineral Supplement Hypochlorous Acid Water (MS-HOCl) has received massive attention in research as a new-concept cleansing agent due to its high sterilizing properties, easy accessibility, and safety aspects. This present study was conducted to evaluate the drinking effects of MS-HOCl treatment of the Helicobacter pylori (H. pylori )-infected C57BL/6 mouse model to verify the safety effects. The experimental mice were divided into 3 groups: NC (Purified water + no infection), PW (Purified water + H. pylori infection), and MS-HOCL (MS-HOCl + H. pylori infection). Water was supplied through water bottle for mouse for 4 weeks, and changed once a day. We measured the bodyweight, and total WBC and its differential counts such as lymphocytes, monocytes, neutrophil, eosinophil and basophil. Besides that, we also tested biochemical markers such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxides (GPx), and calcium activity in both serum and stomach lysate samples. Our results showed that daily oral administration of MS-HOCl water for 4 wk did not show any abnormal findings about hematological and biochemical parameters such as ROS, NO, GPx, and calcium activity in both serum and stomach lysate. There were no significant differences in bodyweight between the three groups. Overall, our results showed no negative effects of drinking MS-HOCl in the H. pylori -infected C57BL/6 mice model. However, more controlled studies are needed to determine the safety of drinking MS-HOCl.

Skin is a complex barrier organ that can fight against various endogenous and exogenous factors. The damaged skin barrier can lead to different skin problems that can exhibit the skin condition such as a decrease of elasticity and sebum level, an increase of water loss, strengthening of keratinocyte cohesion and wrinkle formation, which will eventually lead to different skin diseases. With the current use of chlorine-rich water as disinfectant and its potential in medical field, it is important to check its safety use. The objective of the study was to evaluate skin safety of electrolyzed neutral oxidizing water (ENOW) (pH: 7.11, oxidation reduction potential: 450 mV, available chlorine concentration: 5.0 ppm) after the long-term use (90-day skin application) on the skin of C57BL/6 mice compared to normal control group (NC) treated with tap water (TW) (pH: 7.8, oxidation reduction potential: 500mV, available chlorine concentration: 0.7 ppm). We were measured skin state such as the moisture, elasticity, sebum, pore size, and wrinkle. Oxidative stress markers such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxides (GPx) and catalase (CAT) were measuredin using serum and skin lysate samples. Inflammation cytokines such as IL-1α, IL-1β, IL-13, GM-CSF and TNF-α, and the Th1/Th2 cytokines such as IL-2, IL-4, IL-5, IL-6, IL-10 and IL-12 were also analyzed. In consequently, The skin state of ENOW group showed higher level of elasticity and moisture compared to NC group. ROS and NO levels in skin did not show adverse effect in ENOW group. GPx were also controlled in all groups, however, a significant decrease in CAT level was observed in both NC and ENOW groups. Moreover, serum inflammatory cytokine of ENOW group did not show significant difference as compared to NC and TW groups, however, GM-CSF showed a significantly lower level in the ENOW group than NC group. Cytokines related to allergic response showed no difference in the skin. Taken together, long term treatment of ENOW might be safe on skin in vivo . However, more clinical studies are needed to verify the skin safety of ENOW.