Earticle

To confirm the effect of alkaline water drinking on the red blood cell density, the participants were divided into two groups, purified water (PW) drinking group (n=10) and alkaline water(AW) drinking group (n=10). Two uL of blood was collected before drinking water, and at 10 minutes, 30 minutes, 1 hour, and 2 hours after drinking 15mL/kg within 10 minutes. Blood was on the slide glass under light microscope, red blood cells were counted from taken by 400 times. Also, to confirm the effect of alkaline water drinking on the properties of urine, urine was collected before drinking water and when it feel nature calls after drinking. Urobilinogen, glucose, bilirubin, ketone body, gravity, RBC, protein, WBC, pH and MDA in urine were investigated. Purified water (pH 6.9, +372mV) was produced only by physical filtering using commercial purified filter. AW (pH 9.2, +203.8mV) was prepared by Mineral water ionizer (Blue QQTM, Eagle Eye America Inc., Korea). Red blood cell density was showed lower tendency in AW group than PW group until an hour after drinking, and especially lowest tendency in 30 minutes (p<0.05). But two hours later, it was showed tendency to back to normal. ROS was showed low tendency both AW and TW groups after drinking. AW group was lower than PW group. Urobilinogen, glucose, bilirubin, ketone body and protein were showed low tendency both AW and PW. But pH was showed rising trends both AW (p<0.001) and PW group (p<0.01). Alkaline water is absorbed faster to the blood compared to the tap water and have higher antioxidant effect than PW group.

Rheumatoid arthritis (RA) burden is steadily increasing, while current management options remain limited with regards to undesirable effects, and at times, cost. Early evidence shows that molecular hydrogen (H2) therapy significantly reduces RA disease activity, and H2 may become a safe, effective and inexpensive complement to mainstay therapies. H2‘s unique ability to selectively reduce reactive oxygen species may account for why it is succeeding where other antioxidant strategies have failed. The present work aims to critically appraise the available evidence, mainly the first randomized controlled trial in the field, from an evidence based medicine perspective. Demonstrating the quality of the evidence with a systematic approach will help justify and orient future research efforts. The verdict is the following: despite some methodological shortcomings, H2’s benefits in clinically important outcomes are non-negligible and merit further investigations. Mechanistic studies are needed to improve our understanding of H2 at a molecular level, but thanks to H2’s reported safety, well designed and well executed epidemiological initiatives may be ethically viable and may become powerful assets in building the case for its clinical application.

To confirm the safety and effectiveness of water through far infrared filter, the participants were divided into two groups, general purified water (PW; precarbon filter, hollow fiber membrane filter, and postcarbon filter) drinking group (n=10) and far infrared filter water (FIW; precarbon filter, far infrared filter, and postcarbon filter) drinking group (n=10). All filters made by Dajin filter (Daejin Filter Co., Ltd., Chilgok, Gyeongbuk). All of participants were more than 200 mg/dL in total cholesterol levels and more than 23% in BMI (kg/m2). Drinking amount was 15mL/kg a day for three months. Blood glucose level, blood pressure, blood (CBC, HbA1C, GOT, GPT, T-cho, TG, K, P, Ca), and body fat tests were examined before drinking water, in one month, two months, and three months. In blood glucose test result, glucose level changed to 77.7±7.6 mg/dL in three months from 91.3 ±6.3 mg/dL before drinking in FIW group (p<0.001). However, there were no significant differences in blood pressure, blood test (CBC, HbA1C, GOT, GPT, T-cho, TG, K, P, Ca), and body fat test results. These results considered water through far infrared filter have blood sugar depressing effect, and safe because the water do not have a some impact on human body.

To confirm the antioxidative effect of hydrogen water drinking, the participants were divided into two groups, purified water (PW) drinking group (n=20) and hydrogen water (HW) drinking group (n=20) because of cross test in each 10 in a group. Purified water (pH 6.9, +432mV) was produced only by physical filtering using commercial carbon filter, and hydrogen water (Few Hydrogen Rich WaterTM, Hayannamoo Beverage Co., Ltd., Korea) was used in this experiment (pH 7.5, -650mV, hydrogen 1.5ppm). Fifty uL of blood was collected before drinking water, at 1 and 2 minutes after drinking 12mL/kg. ROS in blood was examined using FORT test kit. ROS was showed lower tendency in HW group than PW group after drinking, and especially lowest tendency in a minute (p<0.01) and 5 minutes (p<0.05). But, there is no the antioxidative effect in purified water. Consequentially, drinking hydrogen water is useful to remove free radicals, ROS.

Non-thermal plasma water has dual function of anti-oxidant effect and anti-microbial effect, so it is useful apply several disease including atopic dermatitis and athlete foot. Atopic dermatitis (AD) is a chronic inflammatory skin disease described not only by the skin-barrier impairment and dysfunctional immune balance but also by increased oxidative stress. The infiltration of immune cells releases bioactive substances including reactive oxygen species. Here, we introduced a potential agent to manage AD in the form of non-thermal plasma water (NTPW) generated by electrical discharger (Plus Co., Ltd.) in water on the regulation of immune markers and antioxidant markers in dinitrochlorobenzene-induced hairless mice. Recent studies found its promising ability of activating, controlling and catalyzing reactions and complex biochemical procedures. NTP brings a big potential in wound healing, such as efficient disinfection or sterilization, therapy of various skin infections or tissue regeneration. In us in vivo experiment, atopic dermatitis was induced by application of 1% DNCB for the first week and alternate 0.5% DNCB application for the following 4-week NTPW treatment. While the regulatory immune and oxidative stress effects of NTP water was found to be more improved than no treatment group which is comparable to the positive controls. NTP water reduced the severity of dermatitis, including erythema/hemorrhage, edema, erosion and scaling, and skin scratching, total WBC specifically the neutrophils and lymphocytes; IgE level is also slightly decreased. ROS and NO in skin lysate showed an improved normalized level comparable to normal control. Accordingly, the antioxidant enzymes including glutathione peroxidase, superoxide dismutase, catalase, and MDA normalized the oxidative stress levels in skin lysate; these could play role in prohibiting AD aggravation. Furthermore, our results suggest that the NTP mechanism of action in AD is regarded with the regulation of immune response in acute phase in AD, and also to the inhibition of neutrophils, lymphocyte, and Th2-mediated IgE overproduction, marking an anti-inflammatory effect. Recent studies depict the association of atopic dermatitis with excessive skin floral invasion especially Staphylococcus aureus. Here, we introduced a possible agent to manage AD in the form of non-thermal plasma (NTP), a partially ionized water where energy is stored in the electron without any change of temperature. Recent studies found its promising effect in different biological application because of its enriched properties of H2O2, OH-, HOCl, O3, and other ions. In this randomized, double blind controlled study, 7 children aged 3 to 17 years old undergone a two-week NTP water bathing intervention in Yeongwol, Republic of Korea. Water samples were obtained aseptically before and after of bathing in NTPW to check the colony forming unit of S. aureus. Previously, in vitro results showed that has remarkable antibacterial and antifungal effect in TW. Before and after skin conditions were also compared by an automated dermatological scale. Our cumulative results showed reduction of Staphylococcus aureus count from patient’s skin swab. In addition, NTPW revealed slightly improved skin condition including moisture, elasticity, roughness and wrinkle. These results could open new horizons in medical dermatology as a management and treatment scale of AD. Overall, our findings strongly recommend that spraying and bathing with NTPW could be a promising therapeutic approach in ROS related disease and skin infectious disease.

수소수와 같은 기능수의 시장이 커짐에 따라 수소수 및 수소수 발생기등 관련 분야의 산업화를 위한 기반 구축이 절실히 요구되고 있다. 기반 구축을 위한 필요한 요소중 하나가 제품 인증을 위한 측정 기술 및 제품 안전을 위한 표준화 이다. 본 세션에서는 물에 용존되어 있는 수소 기체의 함량을 측정할 수 있는 다양한 분석 방법에 대해 검토하고 의견을 수렴하고자 항다. 또한 수소생성기기의 경우 수소의 발생부터 물에 용존시키는 공정까지 안전성 확보를 할 수 있는 요소 기술에 대한 표준 작업이 필요하므로 이에 대한 토의 검토를 통해 공 인적인 절차를 제정하는데 필요한 의견을 수렴하고자 한다.

There is a high demand for industrialization on functional waters including “Hydrogen-water”measurement and a hydrogen generator. In this session we are going to make a open discussion in elucidating possible methods to analyze the hydrogen content absorbed in water quantitatively and evaluating issues on hydrogen generators for its safety. Furthermore we would like to discuss how to standardize these technical issues.