Earticle

목적: 본 연구는 유칼립투스 추출물로 탈색된 모발에 도포하여 모발에 어떠한 영향을 미치는 가를 알아보고자 하였다. 방법: 유칼 립투스 추출물 함량을 0 g, 3 g, 6 g, 9 g을 첨가하여 유칼립투스 제형제를 제조하였다. 제조한 제형제로 8레벨로 탈색한 시료 모발 에 도포하였다. 추출물을 첨가한 제형제로 도포한 시료와 탈색모를 측정하여 비교 분석하였다. 모발의 명도 변화를 알기 위해 색차 계를 이용하여 L* 값을 측정하였다. 또한 탈색된 모발에 영향을 미치는 가를 알기 위해 모발의 인장강도, 메틸렌블루를 이용한 흡광 도, 광택을 측정하였다. 결과: 시료 별 L* 값은 측정 결과 탈색모에 도포한 시료별에서 함량이 높을수록 L* 값의 평균이 높았으며, 8L(3)과 8L(6), 8L(9) 비교 시에는 통계적으로도 유의미한 결과로 명도의 차이가 있었다. 인장강도 측정결과 시료별에서 함량이 높 을수록 인장강도 평균값이 높았으나 통계적으로는 유의미하지 않은 결과로 나타났다. 흡광도 측정결과 시료별에서 평균값과 통계 분석 값의 차이가 있어 유칼립투스 추출물이 탈색모에 영향을 주는 것을 알 수 있었다. 광택 측정결과 평균값에서는 추출물 함량이 증가 할수록 차이를 보였고, 통계적으로 8L(3)을 제외 한 8L(6), 8L(9)에서 차이를 보여 광택에 영향을 미치는 것을 알 수 있었다. 결 론: 탈색모와 추출물을 첨가하여 도포한 시료의 평균값과 통계분석결과 명도, 흡광도, 광택에 있어 차이를 보였다. 유칼립투스 추출 물이 탈색된 모발에 영향을 미치는 것을 알 수 있었다. 차후 다양한 천연 추출물이 탈색된 모발에 영향을 미치는 가를 연구 할 필요가 있다.

Purpose: This study aims to investigate the effects of eucalyptus oil on damaged hair. Methods: Eucalyptus formulations were prepared by adding 0, 3, 6, or 9 g of eucalyptus extract, and these formulations were applied to level 8 bleached hair samples. Various parameters were then measured and compared in the treated bleached hair. The impact of eucalyptus extract on hair brightness was assessed by measuring L* values using a color difference meter. Additionally, tensile strength, absorbance (determined using methylene blue), and gloss of the bleached hair were measured. Results: Measurement analysis showed that L* values increased as the content of eucalyptus extract applied to bleached hair increased. A statistically significant difference in brightness was observed among 8L(3), 8L(6), and 8L(9) samples. Furthermore, average tensile strength of the hair increased as extract content increased, although the difference did not reach statistical significance. Absorbance analysis revealed a significant difference between the average value and statistical analysis value for each sample, indicating that eucalyptus extract affects the absorbance of bleached hair. Gloss analysis also revealed differences in average values as extract content increased, with a significant difference observed at 8L(6) and 8L(9) but not 8L(3), suggesting that eucalyptus extract affects hair gloss in a content-dependent manner. Conclusion: Statistical analysis of average values revealed that eucalyptus extract affected bleached hair in terms of brightness, absorbance, and gloss, with different eucalyptus extract contents having different effects on the hair. Future studies should investigate the effects of various other natural extracts on bleached hair.

目的: 本研究旨在研究桉树油对受损头发的影响。方法: 通过添加 0、3、6 或 9 g 桉树提取物制备桉树制剂，并 将这些制剂应用于8级漂白的头发样品。然后测量并比较经过处理的漂白头发的各种参数。通过使用色差计测量 L* 值来评估桉树提取物对头发亮度的影响。此外，还测量了漂白头发的拉伸强度、吸光度（使用亚甲蓝测定） 和光泽度。结果: 测量分析表明，随着应用于漂白头发的桉树提取物含量的增加，L*值增加。在8L(3)、8L(6) 和8L(9) 样品中观察到亮度存在统计学上的显着差异。此外，头发的平均拉伸强度随着提取物含量的增加而增 加，尽管差异没有达到统计学显着性。吸光度分析显示每个样品的平均值和统计分析值之间存在显着差异，表 明桉树提取物影响漂白头发的吸光度。光泽分析还显示，随着提取物含量的增加，平均值存在差异，在8L(6)和 8L(9)处观察到显着差异，但在8L(3)处没有观察到，这表明桉树提取物以剂量依赖性方式影响头发光泽。结论： 对平均值进行统计分析发现，桉树提取物对漂白头发的亮度、吸光度和光泽度有影响，不同桉树提取物含量对 头发的影响不同。未来的研究应该调查各种其他天然提取物对漂白头发的影响。

목적: 본 연구는 대두유 오일을 이용하여 손상모에 도포 후 모발개선에 미치는 영향이 있는 가를 알아보고자 하였다. 방법: 대두유 추출물 함량을 0 g, 3 g, 6 g, 9 g을 첨가하여 대두유 제형제를 제조하였다. 제조한 제형제로 8레벨로 손상된 모발에 도포하였다. 추 출물을 첨가한 제형제로 도포한 시료와 손상모를 측정하여 비교 분석하였다. 모발의 명도 변화를 알기 위해 색차계를 이용하여 L* 값을 측정하였다. 또한 탈색된 모발에 영향을 미치는 가를 알기 위해 모발의 인장강도, 메틸렌블루를 이용한 흡광도, 광택을 측정하 였다. 결과: 시료 별 L* 값은 측정 결과 손상모에 도포한 시료별에서 L* 값의 평균의 차이가 있고, 통계 결과도 유의미한 결과가 있 어 손상모의 명도 변화에 미치는 영향이 있는 것을 알 수 있었다. 인장강도 측정결과 S(0)과 S(6), S(9) 비교 시 평균의 차이가 있고, 통계 결과도 유의미한 결과가 있어 손상모의 인장강도 변화에 미치는 영향이 있는 것을 알 수 있었다. 흡광도 측정결과 S(0)과 S(6), S(9) 비교 시 평균의 차이가 있고, 통계 결과도 유의미한 결과가 있어 손상모의 흡광도 변화에 미치는 영향이 있는 것을 알 수 있었 다. 광택 측정결과 평균값은 S(0)과 S(6), S(9) 비교 시 평균의 차이가 있고, 통계 결과도 유의미한 결과가 있어 손상모의 광택 변화 에 미치는 영향이 있다는 것을 알 수 있었다. 결론: 손상모에 대두유 추출물을 첨가하여 도포한 시료의 평균값과 통계분석결과 인장 강도, 흡광도, 광택에 있어 차이를 보였다. 결론으로 대두유 추출물이 손상모 개선에 미치는 영향이 있다는 것을 알 수 있었다. 차후 다양한 천연 추출물이 손상모에 어떤 영향을 미치는 가를 더욱 깊이 연구 할 필요가 있다

Purpose: This study was conducted to investigate the effect of the application of soybean oil to damaged hair after. Methods: Soybean oil formulations were prepared by adding 0, 3, 6, and 9 g of soybean oil extract to hair. The prepared formulation agent was applied to the damaged hair at eight levels. The results were measured, compared, and analyzed. To determine the change in the brightness of the hair, the L* value was measured with a chrominance meter. In addition, the tensile strength of the hair, its absorbance using methylene blue, and its gloss were measured to determine whether they affected the bleached hair. Results: There was an effect on the change in brightness of the damaged hair as measured by L* value. Measures of tensile strength showed a difference in the average across S(0), S(6), and S(9), and the results showed statistical significance. The absorbance measurements differed between S(0), S(6), and S(9), showing statistical significance. The differences in average values for gloss were statistically significant across S(0), S(6), and S(9), indicating an effect on the gloss change of the damaged hair. Conclusion: The application of soybean oil extract to damaged hair produced differences in tensile strength, absorbance, and gloss. Thus, soybean oil extract had an effect on the improvement of damaged hair. In the future, and it is necessary to further study how various natural extracts affect damaged hair.

目的: 本研究旨在探讨大豆油对受损头发的修复效果。方法: 将0、3、6和9 g大豆油提取物分别加入头发中，制 备大豆油配方。将制备好的配方涂抹于受损头发上，共设置八个浓度等级。对结果进行测量、比较和分析。为 确定头发亮度的变化，使用色度计测量L*值。此外，还测量了头发的拉伸强度、亚甲蓝吸光度和光泽度，以确定 这些因素是否对漂白后的头发产生影响。结果: L*值测量结果显示，受损头发的亮度有所变化。拉伸强度测量结 果显示，S(0)、S(6)和S(9)组之间的平均值存在差异，且差异具有统计学意义。吸光度测量结果在S(0)、S(6)和 S(9)组之间也存在差异，且差异具有统计学意义。光泽度平均值在S(0)、S(6)和S(9)组之间也存在统计学意义的 差异，表明大豆油提取物对受损头发的光泽度有影响。结论: 大豆油提取物应用于受损头发后，其拉伸强度、吸 光度和光泽度均有所改善。因此，大豆油提取物对改善受损头发具有一定的作用。未来有必要进一步研究各种 天然提取物对受损头发的影响。

목적: 이 연구는 마카다미아 추출물로 손상모에 도포하여 모발 개선에 미치는 효과를 확인하고자 한다. 방법: 마카다미아 추출물 함량을 0 g과 3 g, 그리고 6 g과 9 g을 첨가하여 마카다미아 제형제를 제조하였다. 제조한 제형제를 8레벨로 탈색한 모발 시료에 적 용하였다. 추출물이 포함된 제형제로 도포한 시료와 손상된 모발을 각각 측정하여, 두 집단을 비교 분석하였다. 각 시료의 명도(L*) 변화 차이를 분석 비교하기 위해 색차계를 사용하여 명도(L*)값을 측정하였다. 탈색하여 손상된 모발에 어떠한 영향을 주는 가를 알 기 위해 시료 별 인장강도 측정, 메틸렌블루를 용액을 활용한 흡광도 분석, 모발 표면 광택을 측정하였다. 결과: 각 시료의 명도(L*) 측정 결과 값은 손상모에 도포한 시료에서 함량이 증가할 수록 명도(L*)값 평균의 차이는 있었지만, M(3)과 M(6), M(9) 비교 시에는 통계적으로 의미가 없은 것을 알 수 있었다. 인장강도 측정결과 시료별에서 함량이 높을수록 인장강도 평균값의 차이가 있었고, 통 계적으로 의미가 있는 것을 알 수 있었다. 흡광도 측정결과, 시료별에서 평균값과 통계분석 결과 차이가 있어 마카다미아 추출물이 손상모에 영향을 주는 것을 알 수 있었다. 광택 측정 비교 분석 결과 시료 별 평균값에서 추출물 함량이 높을 수록 차이가 있음을 알 수 있었고, 통계 분석 결과, M(6), M(9) 시료에서 차이가 있어 모발에 영향을 미치는 것을 확인할 수 있었다. 결론: 손상모와 마카다 미아 추출물을 첨가하여 처리한 시료는 인장강도, 흡광도, 광택 측정에서 평균값 및 통계 분석 결과 유의미한 차이를 나타냈다. 결 론으로 본 연구를 통해 마카다미아 추출물이 손상된 모발의 질 개선에 효과가 있음을 확인할 수 있었다. 향후 여러 천연 추출물이 손상모에 어떠한 영향을 미치는지에 대한 추가 연구가 요구된다.

Purpose: This study investigated whether the application of macadamia extract improved damaged hair. Methods: A formulation agent was prepared by adding 0 g and 3 g and 6 g and 9 g of macadamia extract and then applying the agent to sample hair bleached at eight levels. The samples treated with the formulation containing the macadamia extract were measured and compared with the damaged hair for analysis. To determine the hair’s change in brightness, the L* value was measured with a chrominance meter. Additionally, the hair’s tensile strength, and the effects of the treatment on bleached hair were evaluated by measuring both methylene blue absorbance and surface gloss. Results: For each sample, the L* value was found statistically insignificant when compared with M (3), M (6), and M (9). The higher each sample’s application content, the greater the statistically significant difference in average tensile strength. The absorbance measurement showed differences in the macadamia extract’s average value affecting damaged hair due to the difference between the average value and the statistical analysis result for each sample. As the application’s extract content increased, the average gloss measurement differed, and M (6) and M (9) showed statistically significant differences in gloss. Conclusion: The average value of the sample applied to damaged hair, extract amounts, and statistical analysis showed differences in tensile strength, absorbance, and gloss. In conclusion, the macadamia extract’s application improved damaged hair. Future research should investigate how other varied natural extracts affect damaged hair.

目的: 本研究调查澳洲坚果提取物的使用是否可以改善受损头发。方法: 制备配方剂，加入 0 g、3 g、6 g和9 g 澳洲坚果提取物，然后将该配方剂涂抹在8个漂白程度的样品头发上。测量用含有澳洲坚果提取物的配方处理的 样品，并与受损头发进行比较分析。为了确定头发亮度的变化，用色度计测量L*值。此外，还测量了头发的抗拉 强度、亚甲蓝吸光度和光泽度，以确定该应用是否影响漂白头发。结果: 测量了每个样品的L*值，发现涂抹在漂 白头发上的样品含量越高，平均L*值的差异越大，但在比较M(3)与M(6)和M(9)时，这种差异并不具有统计学意 义。抗拉强度测定结果显示，各样品中含量越高，平均抗拉强度的差异越大，且具有统计学意义。吸光度测定 的结果显示，各样品的平均值和统计分析结果存在差异，表明澳洲坚果提取物对受损发质有效果。光泽度测定 结果的平均值随着提取物含量的增加而出现差异，统计上M(6)和M(9)出现差异，表明其对光泽度有影响。结论: 涂抹在受损头发上的样品的平均值、提取物的量和统计分析显示抗拉强度、吸光度和光泽度存在差异。总之， 澳洲坚果提取物的应用改善了受损头发。未来的研究应该调查其他各种天然提取物如何影响受损头发。

목적: 본 연구에서는 Echinacea angustifolia (E. angustifolia)열수 추출물의 화장품 원료로서의 기능성을 확인하기 위해 항산화능 및 항염활성을 측정하였다. 방법: 라디컬 소거능으로서 DPPH, ABTS, FRAP 실험, 항산화 물질 측정으로서 폴리페놀과 플라보노 이드 농도 측정을 실시하였다. 또한, 세포실험에서는 RAW 264.7 cell을 사용하여 세포독성 실험과 항염 실험을 실시하였다. 결과: 실험결과 DPPH 실험에서는 ascorbic acid와 비교 시 27.98%의 항산화능을 나타내었으며, ABTS 실험에서는 ascorbic acid와 비교 시 20.29%의 항산화능을 나타내었다. FRAP에서는E . angustifolia 추출물의 1 mg이 ascorbic acid 0.412±0.013 μg의 환원력을 보 였다. 폴리페놀 농도는 104.42±3.21 mg/g으로 나타났으며, 플라보노이드 농도는 62.86±2.26 mg/g으로 나타났다. 세포실험결 과 세포독성의 경우 80% 이상의 세포가 생존하여 E. angustifolia 추출물이 낮은 독성을 가지고 있음을 확인하였다. 한편 항염 활 성 실험에서도 농도에 따라 염증반응을 감소시키는 동시에 200 μg/mL 농도에서 51.07±4.58%의 염증 억제능을 보였다. 결론: E. angustifolia 추출물의 코스메슈티컬 원료로서의 가능성을 확인할 수 있었다.

Purpose: This study attempted to investigate the antioxidant and anti-inflammatory effects of Echinacea angustifolia extract. Methods: For analysis of radical scavenging activities, DPPH, ABTS and FRAP were performed. To analyze antioxidant properties, in addition, polyphenol and flavonoid concentrations were measured. In cell-based assays, cytotoxicity and anti-inflammatory assays were performed, using RAW 264.7. Results: The results found the followings: DPPH and ABTS assays revealed 27.98% and 20.29% respectively in terms of antioxidant activities when compared to ascorbic acid. In the FRAP assay, E. angustifolia extract (1 mg) showed 0.412±0.013 μg of reducing power (ascorbic acid). Polyphenol and flavonoid concentrations were 104.42±3.21 mg/g and 62.86±2.26 mg/g each. In terms of cytotoxicity, more than 80% cells survived after the assays, confirming the low toxicity of E. angustifolia extract. In anti-inflammatory activities, the extract reduced the inflammatory response and, at the same time, showed 51.07±4.58% inflammationinhibiting effects at 200 μg/mL, confirming the potential of E. angustifolia extract as an ingredient of cosmeceuticals. Conclusion: These results confirmed the potential of E. angustifolia extract as an ingredient of cosmeceuticals.

目的: 本研究试图研究狭叶松果菊（Echinacea angustifolia ，E. angustifolia ）提取物的抗氧化和抗炎作用。方 法: 为了分析自由基清除活性，进行了 DPPH、ABTS和FRAP。此外，为了分析抗氧化特性，还测量了多酚和类 黄酮的浓度。在基于细胞的测定中，使用RAW 264.7 进行细胞毒性和抗炎测定。结果: 与ascorbic acid相比， DPPH和ABTS的抗氧化活性分别高出27.98%和20.29%。在FRAP测定中，E. angustifolia 提取物 (1mg) 显示出 0.412±0.013 μg的还原能力（抗坏血酸）。多酚和类黄酮浓度分别为104.42±3.21 mg/g和 62.86±2.26 mg/ g。细胞毒性检测后，发现超过80%的细胞存活，证实了E. angustifolia 提取物的低毒性。在抗炎活性方面，该 提取物降低了炎症反应，同时在200 μg/mL时，显示出51.07±4.58%的炎症抑制作用，证实了E. angustifolia 提取物作为药妆品成分的潜力。结论: 这些结果证实了E. angustifolia 提取物作为药妆品成分的潜力。

본 연구는 암세포 증식에 미치는 효과를 살펴보기 위하여 Broccoli Extract를 사용하였다. 실험에 사용 한 세포주는 3종으로 호흡기계에 대표적인 폐암세포 A549와 소화기계의 간암세포 SNU-182, 담도암세포 SNU-1196 로 인체 유래 암세포 주를 사용하였으며, 암세포의 증식 억제는 세포의 증식정도를 측정하는 CCK-8 방법을 이용하여 측정하였다. Broccoli Extract 1000ug/mL, 100ug/mL, 10ug/mL 에 대한 암세포 증식 억제 를 살펴본 결과 Broccoli Extract은 대부분의 암세포에서 농도 의존적으로 증식을 억제하였으며, 특히 폐암세포 A549, 간암세포 SNU-182는 Broccoli Extract 1000ug/mL에서 유의한 증식 억제를 보였다. 이러한 결과 브로 콜리 추출물은 세포실험을 통해서 증명된 종양억제기전들이 암 예방 및 치료제로서 잠재력을 제공한다고 볼 수 있다.

This study was conducted to examine the effect of Broccoli Extract on the proliferation inhibition of human-derived cancer cells and the degree of inhibition. The three cell lines used in the experiment were respiratory system lung cancer cells A549, digestive system liver cancer cells SNU-182 and biliary tract cancer SNU-1196. All cancer cells were derived from the human body, and the CCK-8 method was used to measure the degree of inhibition of cancer cell proliferation. As a result of examining the effect on Broccoli Extract 10ug/mL, 100ug/mL, 1000ug/mL, Broccoli Extract inhibited proliferation in a concentration-dependent manner in most cancer cells, In particular, lung cancer cell A549 and liver cancer cell SNU-182 showed significant proliferation inhibition at 1000ug/mL.As a result, it can be seen that broccoli extract provides potential as a cancer preventive and therapeutic agent for tumor suppression mechanisms proven through cell experiments.

본 연구는 방울양배추 추출물의 다양한 암세포 증식에 미치는 영향을 살펴보고 증식 저해 정도를 살펴보기 위하여 실시되었다. 실험에 사용한 세포주는 6종이며 전립샘암 세포주인 DU-145, 폐암 세포주인 A549, 유방암 세포주인 MCF-7, 자궁암 세포주인 HeLa, 담도암 세포주인 SNU-1196, 간암 세포주인 SNU-182 로 모두 인체에서 유래된 암 세포주를 사용하 였다. 암세포의 증식 저해는 CCK-8 assay를 이용하여 측정하였다. 방울양배추 추출물 1ug/mL, 10ug/mL, 100ug/mL에 대한 암세포 증식 저해를 살펴본 결과 폐암세포 A549, 자궁암세포 HeLa는 방울양배추 추출물 10ug/mL, 100ug/mL에서 유 의한 증식 저해를 보여주었으며, 담도암세포 SNU-1196는 방울양배추 추출물 1ug/mL, 10ug/mL, 100ug/mL에서 유의한 증식 저해를

This study was conducted to examine the effect of Brussels sprout extract on the proliferation of various cancer cells and to examine the degree of inhibition of proliferation. The six cell lines used in the experiment were human-derived cancer cell lines: prostate cancer cells DU-145, lung cancer cells A549, breast cancer cells MCF-7, uterine cancer cells HeLa, biliary tract cancer cells SNU-1196, and liver cancer cells SNU-182. Inhibition of cancer cell proliferation was measured using the CCK-8 assay. As a result of examining the inhibition of cancer cell proliferation by 1ug/mL, 10ug/mL, and 100ug/mL of Brussels sprout extract, lung cancer cell A549 and uterine cancer cell HeLa showed significant proliferation inhibition at 10ug/mL and 100ug/mL of Brussels sprout extract, and biliary tract Cancer cells SNU-1196 showed significant inhibition of proliferation at 1ug/mL, 10ug/mL, and 100ug/mL of Brussels sprout extract. These results suggest that Brussels sprout extract may have potential anticancer effects against various types of cancer cells.

본 연구는 염색 전에 참깻묵 추출물과 Bacillus subtilis 균주로 발효한 발효물을 처리했을 시 색상 지속력에 영향을 미치는지 확인하고자 하였다. 화학 시술을 전혀 하지 않은 건강한 20대 여성의 모발을 절단 채취하여 정상모 (N), 1회 탈색모(B1), 2회 탈색모(B2) 시료를 준비하였다. 전처리를 하지 않은 대조군(Cont), 참깻묵 추출물을 전처리 한 실험군(S), 발효물을 전처리한 실험군(FS)으로 나누고, 적색 염색과 청색 염색 후 샴푸 횟수에 따른 염색 지속성을 확인하였다. 실험은 육안평가, 다공성(흡광도)평가, L*a*b* 측정을 진행하였다. 그 결과 육안으로는 큰 변화가 없었고, 다공성평가에서는 대조군 보다 실험군의 염료 탈착이 적은 것을 확인하였다. 또, 색차 측정의 결과 S군과 FS군은 Cont군과 비교하여 적색 염색의 경우 a*값, 청색 염색의 경우 b*값의 유지에 도움이 되었다. 본 연구 결과를 통해, 참깻묵의 추출물과 발효물이 모발을 보호하고, 염색의 지속력을 향상시키는 것을 확인하였다.

This study aimed to investigate the effect of treating hair with perilla meal extract and its fermented product with Bacillus subtilis on color retention. Hair samples were collected from healthy women in their 20s with no prior chemical treatments and were categorized into three groups: untreated hair (N), hair bleached once (B1), and hair bleached twice (B2). The samples were further divided into a control group with no pretreatment (Cont), an experimental group pretreated with perilla meal extract (S), and an experimental group pretreated with the fermented product (FS). After dyeing with red and blue dyes, color retention was evaluated based on the number of shampoos. The experiment included visual assessment, porosity (absorbance) evaluation, and Lab* measurements. Results showed no significant visual changes, but the porosity evaluation indicated less dye detachment in the experimental groups than in the control group. Additionally, color difference measurements showed that the S and FS groups, compared to the Cont group, were better at retaining the a* value for red dye and the b* value for blue dye. These findings confirm that perilla meal extract and its fermented product help protect hair and improve dye retention.

목적: 피부변화 현상을 늦추고, 젊고 산뜻한 외모를 가꾸려는 사회적 요구에 맞춰 최근 화장품의 연구동향은 새로운 천연 원료 개발 과 상용화에 집중되고 있다. 본 연구는 화장품 원료로써 비타민나무열매 오일과 열수 추출물의 피부 눈가주름, 안면 볼 리프팅, 피 부 탄력, 피부 치밀도, 피부 톤, 기미 및 색소 침착 완화 개선에 대한 인체효능을 평가하여 화장품의 새로운 천연 원료로 검증 하는 데 목적이 있다. 방법: 본 연구는 30-60세의 성인 여성 20명을 대상으로 비타민나무 열매 오일과 열수 추출물의 피부 개선 효능을 검증하기 위하여 인체적용시험을 실시하였다. ANTERA 3D를 적용하여 눈가주름 개선, 안면 볼 리프팅 개선, 기미 및 색소침착완 화를 평가하였고, Ballistometer를 이용하여 피부 탄력 개선을 평가하였고, DUB® SkinScanner를 적용하여 피부 치밀도 개선을 평 가하였고, 또한 분광광도계 및 전안촬영시스템을 적용하여 피부 톤 개선을 평가하였다. 결과: 시험물질 도포부위에서 눈가주름 개 선율은 3주 사용 후 6.75%, 6주 사용 후 12.86%로 나타났고, 안면 볼 리프팅 개선율도 시험물질 도포부위에서 3주 사용 후 31.15%, 6주 사용 후 44.63%, 피부 탄력 개선율은 3주 사용 후 2.46%, 6주 사용 후 4.28%로 나타났다. 시험물질 도포부위에서 피부 치밀도 개선율은 3주 사용 후 6.12%, 6주 사용 후 9.63%로 나타났고, 피부 톤 개선율도 시험물질 도포부위가 3주 사용 후 1.12%, 6주 사 용 후 1.63%, 기미 및 색소 침착 완화 개선 개선율도 시험물질 도포부위에서 3주 사용 후 1.60%, 6주 사용 후 2.46%로 나타났다. 결 론: 이상과 같이 비타민나무 열매 오일과 열수 추출물의 피부 눈가주름, 안면 볼 리프팅, 피부 탄력, 피부 치밀도, 피부 톤, 기미 및 색소 침착 완화 개선 효과를 검증하였고 비타민나무 열매 오일과 열수 추출물이 새로운 피부 항노화 화장품 원료로써 사용 될 수 있 는 것으로 사료된다.

Purpose: In this study, a human application test was performed to verify the skin improvement effect of Hippophae rhamnoides (H. rhamnoides ) fruit oil and hot-water extracts to improve wrinkles around the eyes, facial cheek lifting, skin elasticity, skin density, skin tone, spots and pigmentation as ingredients for cosmetics. To meet this purpose, this study has focused on the development and commercialization of new natural ingredients. Methods: This study conducted a human application test to verify the skin improvement effect of H. rhamnoides fruit oil and hot-water extracts in 20 adult women aged 30-60 years old. By applying ANTERA 3D, we evaluated wrinkles around the eyes, improved facial cheek lifting, and spots and pigmentation, evaluated skin elasticity improvement using a Ballistometer, and evaluated skin density improvement by applying DUB® SkinScanner. The skin tone improvement was evaluated by applying a photometer and an anterior eye imaging system. Results: After 3 and 6 weeks of treatment, the rates of improvement at the areas where the test material was applied were respectively 6.75% and 12.86% at the wrinkles around the eyes, 31.15% and 44.63% for facial cheek lifting, 2.46% and 4.28% for skin elasticity, 6.12% and 9.63% for skin density, 1.12% and 1.63% for skin tone, and 1.60% and 2.46% for spots and pigmentation. Conclusion: As a result, the effects of H. rhamnoides fruit oil and hot-water extracts for reducing skin wrinkles, blemishes, and pigmentation, and improving facial cheek lifting, skin elasticity, density, and skin tone were verified. We believe that H. rhamnoides fruit oil and hot-water extracts can be used as a new skin anti-aging cosmetic ingredient.

目的: 近在这项研究中，进行了一项人体应用测试，以验证沙棘果油和热水提取物对改善眼睛周围的皱纹，提拉 脸颊，皮肤弹性，皮肤密度，肤色，色斑和色素沉着的皮肤改善作用。为了达到这个目的，本研究集中于新天 然成分的开发和商业化。方法: 本研究对20名30至60岁的成年女性进行了一项人体应用测试，以验证沙棘果油 和热水提取物的皮肤改善作用。通过使用ANTERA 3D，我们评估了眼睛周围的皱纹，改善了脸颊的提拉，斑点 和色素沉着，使用Ballistometer评估了皮肤弹性的改善，并通过使用DUB®SkinScanner评估了皮肤密度的改 善。通过使用光度计和前眼成像系统评估肤色改善。结果: 治疗3周和6周后，在使用测试材料的区域中，眼部 皱纹的改善率分别为6.75％和12.86％；脸颊提拉的改善率分别为31.15％和44.63％；皮肤弹性的改善率分别为 2.46％和4.28％；皮肤密度为6.12％和9.63％；肤色为1.12％和1.63％；斑点和色素沉着改善率分别为1.60％ 和2.46％。结论: 结果证实了沙棘果油和热水提取物对减少皮肤皱纹，斑点和色素沉着，改善脸颊提拉，皮肤弹 性，密度和肤色的作用。因此充分可以用作一种新型的皮肤抗衰老化妆品成分。

아로니아 비가식 부위(잎, 송이가지, 가지)를 바이오 기능성 소재로 활용하기 위하여 부위별, 용매별 추출물 의 총 폴리페놀 함량과 생리활성을 분석하였다. 잎과 가지 추출물의 폴리페놀 함량은 열매와 송이가지 추출물보다 더 높은 값을 나타냈고, 아로니아의 부위별 항산화능(DPPH 래디컬과 ABTS 래디컬 소거능)을 분석한 결과, 열수추 출물과 메탄올추출물 두 경우에서 가지, 잎, 열매, 송이가지의 순으로 활성을 나타냈다. Tyrosinase 저해활성을 평가한 결과, 열수추출물은 열매, 잎, 가지, 송이가지의 순으로 높은 저해활성을 보였으며, 메탄올추출물의 경우 가지, 잎, 열매, 송이가지의 순으로 저해활성을 갖는 것으로 나타났다. Nitric oxide 저해활성은 열수추출물과 메탄 올추출물 두 경우에서 열매, 잎, 가지, 송이가지의 순으로 높았다. 결론적으로 아로니아 가지와 잎은 항산화, 미백 및 항염증 활성을 갖고 있는 것으로 확인하였고, 추가적인 연구를 통하여 화장품 등의 바이오 헬스케어 제품의 기능성 바이오소재로 개발할 수 있을 것으로 기대된다.

Total polyphenol content and biological activities of water and methanol extract from Aronia melanocarpa berry, leaf, stem and twig were analyzed to investigate the potential for a bio-functional material. The polyphenol content of leaf and twig extracts were higher than those of berry and stem extracts. DPPH radical and ABTS radical scavenging activities of both extracts, were measured in order of twig, leaf, berry and stem. Tyrosinase inhibitory activities of water extract were measured in order of berry, leaf, twig and stem. Those of methanol extract were measured in order of twig, leaf, berry and stem. The nitrite-scavenging ability of water and methanol extract were measured in order of berry, leaf, twig and stem. Consequently it was shown that the non-edible parts of aronia, leaf and twig, had antioxidant activities and nitric oxide scavenging activities. It is expected that these materials could be used as functional bio-materials in bio-health care products including cosmetic products.

본 연구에서는 과일즙이 복합레진의 표면부식과 착색에 미치는 영향을 알아보고자 일상적으로 많이 섭취하 는 과일 중 산도가 높고 색깔이 있는 포도, 레몬, 오렌지, 자몽, 키위 5종을 선정하고 레진시편을 제작하여 과일즙에 침지하였다. 레진시편은 37℃에서 과일즙에 3일, 7일 간격으로 침지하였다. 대조군은 과일즙에 침지하지 않은 레진으 로 하였다. 시간에 따른 표면의 변화는 주사전자현미경을 이용하여 관찰하였으며 착색정도의 변화는 분광광도계를 이용하여 측정하였다. 주사전자현미경으로 레진의 표면변화를 관찰한 결과, 대조군에 비해 실험군은 표면 입자간의 홈의 크기가 증가하였고 직사각형모양의 막대상의 구조가 생겨났으며 작게 돌출된 돌기상의 입자가 사라지는 양상을 보였다. 분광광도계로 레진의 착색정도를 측정한 결과, 실험군 간의 차이는 포도즙에 침지한 경우 명도가 가장 어두 운 것을 볼 수 있었다.

At this study, we select five colorful and high-acid fruits: grape, lemon, orange, grapefruit and kiwi, and we made a composite resin specimen and dipped into fruit juice in order to figure out influence that affects on surface corrosion and coloring. We dipped resin specimen into fruit juice by three-day and seven-day interval at 37℃. Control group used the resin that was not dipped into fruit juice. For surface change according to the time, it was observed by using scanning electron microscopy, and it was measured by using spectrophotometer for change of coloring degree. As a result of surface change of resin by using scanning electron microscopy, furrow size between surface particles increased, and rectangular rod-shape structure was emerged, and small prominent particles on bumps disappeared. As a result of coloring degree of resin by measuring spectrophotometer, a difference between experimental group appears with the darkest brightness in the case where it is dipped into grape extracts.

Background: Philadelphus schrenkii Rupr. is a widespread in East Asia and has historically been used as a medicinal herb in Korea to reduce heat, swelling, and pain. This study investigated the anti-inflammatory effects of P. schrenkii methanol extract (PSE) on lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Methods and Results: PSE inhibited the expression of nitric oxide, prostaglandin E2, inducible nitric oxide synthase, and cyclooxygenase-2 at concentrations that did not affect cell viability as determined by the tetrazolium salt (WST-1) assay. The quantitative reverse transcription poly- merase chain reaction and enzyme-linked immunosorbent assay measured that pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and monocyte chemoat- tracted protein-1, were decreased in PSE-treated groups. Western blotting confirmed that PSE inhibited the phosphorylation of nuclear factor kappa B and mitogen-activated protein kinases. Gas chromatography-mass spectrometry analysis revealed the presence of active anti-inflammatory compounds in PSE. Conclusions: This study demonstrated that PSE has potent anti-inflammatory activity in vitro and pro- vides a reasonable basis for the traditional use of P. schrenkii in treating inflammation-related diseases.

Elsholtzia fruticosa (EF) is present in tropical regions throughout South Asian countries as well as the Himalayas. Although it has been used as a traditional medicine to treat digestive, respiratory, and inflammatory issues, its effect on preadipocyte differentiation is unknown. In this study, we examined the effects of a methanol extract prepared from EF on the differentiation of 3T3-L1 preadipocytes. Cell differentiation was assessed by microscopic observation and oil-red O staining. The expression of adipogenic and lipogenic genes, including PPARγ and C/EBPα, was measured by western blot analysis and quantitative real-time polymerase chain reaction (qRT?PCR), to provide insight into adipogenesis and lipogenesis mechanisms. The results indicated that EF promotes the differentiation of 3T3-L1 preadipocytes, with elevated lipid accumulation occurring in a concentration-dependent manner without apparent cytotoxicity. EF enhances the expression of adipogenic and lipogenic genes, including PPARγ, FABP4, adiponectin, and FAS, at the mRNA and protein levels. The effect of EF was more pronounced during the early and middle stages of 3T3-L1 cell differentiation. Treatment with EF decreased C/EBP homologous protein (CHOP) mRNA and protein levels, while increasing C/EBPα and PPARγ expression. Treatment with EF resulted in the upregulation of cyclin E and CDK2 gene expression within 24 h, followed by a decrease at 48 h, demonstrating the early-stage impact of EF. A concomitant increase in cyclin-D1 levels was observed compared with untreated cells, indicating that EF modulates lipogenic and adipogenic genes through intricate mechanisms involving CHOP and cell cycle pathways. In summary, EF induces the differentiation of 3T3-L1 preadipocytes by increasing the expression of adipogenic and lipogenic genes, possibly through CHOP and cell cycle-dependent mechanisms.

Background: Alzheimer’s disease (AD) is a major neurodegenerative disorder and major type of dementia with progressive cognitive deficits, including multifactorial disorders. The present study examined the therapeutic effect of Sedum kamtschaticum leaf extract (SKE) on cognitive dysfunction disorder induced in rats through trimethyltin (TMT) administration. Methods and Results: In the preliminary screening experiment, SKE was selected as an acetylcholinesterase (AChE) inhibitor. Rats in the negative control and SKE groups were intraperitoneally injected with TMT (8 ㎎/㎏ body weight) dissolved in 0.9% saline and normal rats were injected with 0.9% saline alone. Doses of SKE (100, and 300 ㎎/㎏) were orally administered to the rats for 14 days. Normal and control rats were treated with saline for the same period. We performed a behavioral test using a Morris water maze and found that the escape and latency time periods of the SKE300 rats were shorter than those of the control rats. SKE therefore ameliorated cognitive dysfunction in this animal model. Immunohistochemical assay of biomarkers in the hippocampal cells revealed that the cell levels of phosphorylated cAMP response element binding protein (p-CREB) and AChE in the CA3 region of the control group were lower than those in the normal group. However, p-CREB and AChE reactive hippocampal cells in SKE300 group were significantly increased compared to those of the control. The hippocampal cells of brain-derived neurotrophic factor (BDNF) and protein kinase C (PKC) in control rats tended to be lower than the normal group, while the cells in the SKE groups tended to be higher than the control group. Conclusions: These results suggest that SKE ameliorates cognitive disorders through the neuroprotective effects related to p-CREB and AChE in the hippocampal cells.

Buckwheat (Fagopyrum esculentum) has been known for having strong anti-oxidant, anti-mutagenic, and anti-carcinogenic activities. The free radical theory of aging, also known as the oxidative stress theory of aging, claims that cellular oxidative damage accumulated with time is a major causal factor of aging. In the present study, we investigated the effect of buckwheat extracts on resistance to oxidative stress and aging using Caenorhabditis elegans as a model system. Survival under an oxidative-stress condition induced by paraquat increased markedly following 500㎎/L buckwheat extracts treatment, suggesting lower cellular oxidative damage by buckwheat extracts. A lifespan assay also revealed that treatment of buckwheat extracts significantly extended both the mean and maximum lifespan in C. elegans. Interestingly, this lifespanextension by buckwheat extracts was not accompanied by reduced fertility. These findings suggest that buckwheat extracts can confer longevity phenotype to C. elegans through its strong anti-oxidant activity and support the aging theory which emphasizes a pivotal role of oxidative stress during aging.

The present study investigated an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix for possible neuroprotective effects on neurotoxicity induced by amyloid β protein (Aβ) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to 10 μM Aβ (25-35) for 36 h induced neuronal apoptotic death. At 1-50㎍/㎖, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) induced by Aβ (25-35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of ICR mice with 15 nmol Aβ (25-35) was inhibited by chronic treatment with HS0608 (25, 50 and 100 ㎎/㎏, p.o. for 7 days) as measured by a passive avoidance test. From these results, we suggest that the antidementia effect of HS0608 is due to its neuroprotective effect against Aβ (25-35)-induced neurotoxicity and that HS0608 may have a therapeutic role in preventing the progression of Alzheimer’s disease.

Paeoniae radix has been widely used for its anti - allergic, anti - inflammatory and analgesic effects, and demon strated to have anticonvulsant, memory enhancing and anxiolytic activities. The present study was performed to examine the protective effect of methanol extract of Paeoniae radix (PR) from Paeonia Japonica Miyabe et Takeda (Paeoniaceae) on hydro gen peroxide (H 2 O 2 ) - induced neurotoxicity using cultured rat cerebral cortical neuron. H 2 O 2 produced a concentration - depen dent reduction of neuronal viability. PR, over a concentration range of 10 to 100 ㎍ / ㎖ showed concentration - dependent decrease of the H 2 O 2 ( 1 0 0 µ M ) - induced neuronal cell death, as assessed by a 3 - [4,5 - dimethylthiazol - 2 - yl] - 2,5-di - phenyl - tetrazo lium bromide assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. PR (100 ㎍ / ㎖ ) inhibited 100 µM H 2 O 2 - induced elevation of the cytosolic Ca 2+ concentration ([Ca 2+ ] c ), which was measured by a fluorescent dye, fluo - 4 AM. PR (50 ㎍ / ㎖ ) i n h i b i t e d g l u t a m a t e r e l e a s e i n t o m e d i u m i n d u c e d b y 1 0 0 µ M H 2 O 2 , which was measured by HPLC, and generation of reactive oxygen species (ROS). These results suggest that PR may mitigate the H 2 O 2 - induced neurotoxiciy by interfering with the increase of [Ca 2+ ] c , and then inhibiting glutamate release and generation of ROS in cultured neurons.

Polygalae Radix (PR) from Polygala tenuifolia (Polygalaceae) is traditionally used in China and Korea, since this herb has a sedative, antiinflammatory, and antibacterial agent. To extend pharmacological actions of PR in the CNS on the basis of its CNS inhibitory effect, the present study examined whether PR HAS THE NEURONS. PR, over a concentration range of 0.05 to 5㎍/㎖ , inhibited KA (500μm)-induced neuronal cell death, which was measured by a trypan blue exclusion test and a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-terazolium bromide (MTT) assay. PR (0.5 ㎍/㎖)ingibited glutamate release into medium induced by KA (500 μM), whcih was measured by HPLC. Pretreatment of PR(0.5㎍/㎖) inhibited KA (500 m)-induced elevation of cytosolic calcium concentration([Ca2+]c), which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that PR prevents KA-induced neuronal cell damage in vitro.

본 배경 : 질염은 항생제를 복용하는 방식으로 치료를 하고 있으며, 이러한 항생제의 지속적인 치료는 내성을 유발할 수 있다. 연구 방법 : Lactic acid bacteria 2종에 에센셜 오일을 이용한 항균 활성을 보고자 한다. SE(Grapefruit Seed Extract), eucalyptus, tea tree, clove bud oil, cinnamon oil, lemongrass oil, thyme oil, ginger oil을 일정 비율로 넣어 배양 후, 병원성 미생물- E. coli, C. albicans와 Lactic acid bacteria은 균주에 맞는 배지를 사용하여 균 수를 측정 하였다. 결과 : Essential oil 7종과 GSE가 병원성 미생물에 억제 효과가 있으며, 병원성균(E. coli, C. albicans)에 대한 Grapefruit seed extract(GSE)의 억제농도를 확인하였다, 병원성균은 억제하고 Lactic acid bacteria는 억제하지 않는 배합비도 확인하였다(GSE 농도가 200ppm에서 Eucalyptus globulus(EG) oil 50㎕와 Melaleuca alternifolia oil(Tea tree oil, TTO) 50㎕(pH 5.0, 5.5, 6.0)). 결론 : 본 실험에서 Essential oil은 다양한 항균 활성 가지고 있어 항생제 대안으 로도 생각할 수 있으며, 장기 항생제 치료환자에 대한 보조 항균제로서도 유용할 것으로 생각된다.

Objectives: Antibiotics help treat Vaginitis, and prolonged usage of antibiotics can lead to resistance. Methods: This study investigates the antimicrobial activity of two types of lactic acid bacteria using essential oils. After cultivation by adding grapefruit seed extract (GSE), eucalyptus, tea tree, clove bud, cinnamon, lemongrass, thyme, and ginger oils in a specific ratio, pathogenic microorganisms, namely E. coli, C. albicans, and lactic acid bacteria were released. The number of bacteria was measured using a medium suitable for the strains. Results: The essential oils and GSE inhibited pathogenic microorganisms, and the inhibitory concentration of GSE against pathogenic bacteria (E. coli, C. albicans) was confirmed. The non-inhibitory mixing ratio was also confirmed (50 μl of eucalyptus globulus (EG) oil and 50 μl of melaleuca alternifolia oil (tea tree oil, TTO) at 200 ppm GSE (pH 5.0, 5.5, 6.0)). Conclusion: Essential oils can be considered as an alternative to antibiotics because of their antibacterial properties. They are useful as auxiliary antibacterial agents for patients under long-term antibiotic treatment.

본 연구의 목적은 건강한 모발을 유지하기 위해 트리트먼트로 모발을 유지 관리하기 위해서이다. 잦은 퍼머넌트 로 인한 모발 끝에 많은 손상을 가져오는데 손상된 모발 끝을 보호하기 위해 단계별로 퍼머넌트 웨이브 전·후 처리제를 사용한다. 본 연구에 사용된 은행잎 추출물은 항균, 항산화 및 항암에 효과적이고 혈액순환과 피부보습 효과가 있다. 이 추출물을 파마지 1장과 파마지 2장에 적셔 와인딩한 후 큐티클, 인장강도 및 웨이브 형성률을 알아보고자 한다. 평균비교분석을 하였으며, 파마지 2장에 은행잎 추출물을 적용하였을 때 퍼머넌트한 모발끝이 모발개선효과가 가장 높은 것으로 나타났다.

The purpose of this study is to maintain healthy hair by treatment. Frequent permutations cause a lot of damage to the ends of the hair, and use permant wave pre- and post-processing agents step by step to protect the damaged ends of the hair. The Ginkgo Biloba Leaf Extract used in this study are effective for anti-bacterial, antioxidant, anti-cancer blood circulation and skin moisturizing. This extract was soaked in 1 perm paper and 2 perm papers and wound, and then the cuticle, tensile strength and wave formation rate were investigated. An average comparison analysis was conducted, and when the ginkgo leaf extract was applied to two perm paper sheets, the permanent hair tip showed the highest hair improvement effect.