Earticle

본 연구는 광고크리에이티브 전략으로 활용되는 스토리텔링의 메시지 효과에 관한 것으로, 광고에서 스토리텔링 유형이 광고에 대한 태도, 스토리에 대한 태도, 브랜드에 대한 태도에 어떠한 영향을 탐색해 보는 것을 연구목적으로 한다. 이를 바탕으로 스토리텔링 광고 전략의 접근 방향도 제시하였다. 20, 30대 남녀 420명을 대상으로 3가지 형태의 인쇄광고물을 보여주고 실험 한 결과는 다음과 같다. 첫째, 스토리텔링 유형에 따라 광고태도, 스토리 태도, 브랜드 태도가 차이가 났다. 둘째, 광고 태도, 스토리 태도, 브랜드 태도에 있어 패션관여도와 스토리텔링 유형 간의 상호작용성을 분석한 결과, 스토리텔링 유형의 주효과만 나타났다.

The purpose of this study is to investigate the influences of the story-telling types on the consumer's attitudes towards the advertising and the brand. Three advertisements with different story-telling types were shown to 420 subjects. Meaningful results are found out as follows. First, there is a significant difference in liking and understanding for the ads by storytelling types. And, there is a significant difference between story interest and story understanding by storytelling types. There are same patterns of effects on brand attitudes. A few potentials values of the story-telling strategy in advertising creative strategy are proposed for the future study.

Kim, Jeong-Eun. 2009. Korean language teachers' intercultural competence and Intercultural education methods for teachers. The Language and Culture 5-3: 75-97. The context of the Korean language classroom is the place where diverse people with different cultures and languages can communicate among various cultural backgrounds. Thus, this requires the teacher to be qualified with intercultural competence in order to help Korean learners understand and negotiate their conflicts, because in that context, the teacher's correlational ability must have been reflected in the aspects of respecting and recognizing learners from various backgrounds as well as all the curriculums of language learning. Therefore, this study surveys intercultural competence of Korean teachers, interacting with diverse people between different cultures, and presents the programs for intercultural learning. To do this, the research will be as follow: First of all, after this study looks into the curriculums of Korean academic degrees, 'Education of Korean as a foreign Language', it observes how the intercultural education for teachers has been working. In addition, this study is intended for implications, by monitoring the curriculums of developing the vernacular in contents of the standard education, based mainly on the communication, in Japan. Moreover, this focuses on the main points, which several researchers have suggested, developing intercultural competence for communicating effectively between cultures. Based on these materials, by means of the survey instrument consisted of interview and questionnaire, this research examines and analyzes intercultural competence of the Korean educators as following: knowledge and experiences about intercultures, communicative skills between different cultures, and interactive correlational abilities. The result of the analysis is showed with methods of the intercultural teaching required Korean educators, which are divided into 'culture-specific' and 'culture-general". (Sookmyung Women's University)

과학영재교육의 주요한 목적은 과학영재들이 성장해서 그들의 영재성을 발휘하도록 하는 것 에 있다. 무엇보다도 과학영재들의 과학과 과학자에 대한 인식은 그들의 진로나 과학에 대한 사고방 식에 큰 영향을 줄 수 있기 때문에 과학자로서의 도전감이나 그것을 위한 준비에 대한 생각 등 구체 적인 과학영재들의 사고를 파악하는 것이 필요하다. 이에 본 연구에서는 ‘My Dream Job' 프로젝트를 통해 과학영재들의 미래 자신의 직업에 대한 인식과, 과학에 대한 도전의식을 파악하고자 하였다. 대 부분의 영재들이 현재 자신들이 속해 있는 전공분야를 지속적으로 발전시키기를 원하고 있었으나, 소수 의견 중에는 의사 또는 인문분야의 직업을 선택하겠다고 대답한 학생들도 있었다. 이것이 잘 못 되었다고 볼 수는 없으나, 과학기술분야의 인재 육성을 성공적으로 달성하기 위해서는 과학영재들 의 과학 분야에 대한 도전의식을 고취시키고 유지시킬 수 있는 교육적 자극과 교수전략에 대한 연구 가 필요하다.

The principal purpose of science gifted education is enhance up their ability in gifted. It is needed to find out their thinking about scientific challenge that is give influence in their course or scientific mind. In this study, we want to find out their challenging mind in science and their cognition about future job through the 'My Dream Job Project'. Almost students of science gifted are wants they can make development their subject area. Achieving to make capable man in science fields, we need to study about educational stimulation and teaching skill.

This study suggests a plan to apply head movements to Korean culture class to highlight the importance of nonverbal communication in educational aspect. Nonverbal communication involves those nonverbal stimuli in a communication setting that are generated by both the source [speaker] and his or her use of the environment and that have potential message value for the source or receiver [listener]. Basically it is sending and receiving messages in a variety of ways without the use of verbal codes (words). It is both intentional and unintentional. Most speakers/listeners are not conscious of this. In this paper, we explore nonverbal characteristics of Korean traditional “Bongsan mask dance”, focusing on head movements. Conclusions derived from this study are followings: The function of nonverbal communication in the “Bongsan mask dance” represents repeating, contradicting, complementing, accenting, substituting, and regulating. This study paid attention to this aspect and attempted to implement values of nonverbal communication in Korean culture class.

Ganoderma lucidum 균사체의 액체 배양을 통하여 균체 내 sphingolipid를 분리 정제하여 그 구조를 규명하였으며, 피부보습을 비롯한 각종의 기능성을 탐색하여 화장품 소재 및 새로운 생물 산업소재의 이용가능성을 검토한 결과 배양한 Ganoderma lucidum로부터 일련의 과정을 통하여 crude sphingolipids를 얻고, methanol침전, Dowex AG 50W-X8(H2) cation exchange chromatography 및 preparative thin layer chromatography를 행하여 sphingolipids를 분리하였다. 생산량은 0.4g/L였으며, 수율은 1%였다. UV/VIS spectrum, FT-IR 및 1H-NMR 분석을 행하여 구조를 해석한 결과 phytosphingosine 유도체로 판명되었다. 이 phytosphingosine 유도체의 피부에 대한 보습효과는 500μg/mL 이상의 농도에서 약 20% 정도 skinmate 값이 증가하였으며, 100μ/mL 이상의 농도에서 거칠기 감소에 유의한 결과를 나타내었다. 이상의 결과로부터 Ganoderma lucidum이 생산하는 sphingolipids는 피부보습 및 거칠기 개선에 유의성이 확인되어 화장품 소재 및 새로운 생물 산업소재로서의 이용가능성이 높다고 할 수 있다.

Sphingolipid production was investigated through Ganoderma lucidum-submerged cultivation. Crude sphingolipid obtained from G. lucidum was purified by methanol precipitation, Dowex AG DW-X8 (H+ form) cation exchange chromatography, and preparative thin layer chromatography, Structure and functionalities of purified sphingolipid were elucidated including cutaneous hydration effect. Possibility of use as cosmetics material and new biomaterial was explored. Production was 0.4 g/L at 1% yield. Purified sphingolipid was identified as D-ribo-1,3,4-trihydroxy-2-aminoocta decan through UV/VIS, FT-IR, and 1H-NMR. Sphingolipids increased skinmate value for cutaneous hydration effect by 20% at 500 μg/mL and decreased skin roughness at 100 μg/mL. Results suggest shingolipids from G. lucidum are effective for cutaneous hydration and improvement of skin roughness.

Objective: This study examined the effects of participation in the Yeonje Parenting Dads Program on changes in fathers' parenting attitudes. Methods: The participants included 33 fathers who completed the Yeonje Parenting Dads Program, of whom 13 also participated in in-depth interviews. The program comprised parenting education, parent-child experiential activities, and online mission-based tasks. Quantitative data were analyzed using paired-sample t-tests with the Parenting Attitudes Inventory, while qualitative data from post-program individual in-depth interviews were subjected to thematic analysis. Results: Quantitative findings indicated a significant increase in supportive expressions and significant decrease in achievement pressure, punishment, and inconsistency, reflecting a shift toward more emotionally supportive and consistent parenting attitudes. Qualitative findings revealed that fathers transitioned from an authoritarian and directive parenting style to a relationship-centered approach that values children's emotions and opinions, and they redefined their roles as active co-parents. Conclusion/Implications: The findings demonstrate the effectiveness of a community-based fatherhood program in enhancing fathers' engagement and positive parenting attitudes. These results underscore the need for the continued development of father-focused parenting education programs and for policy support to expand and sustain such community-based interventions.

흑효모(Aureobasidium pullulans)로부터 추출한 베타글루칸(β-glucan)은 기능성 식품 및 의약품 소재로의 활용 가능성이 높아 주목받고 있는 천연물 중 하나이다. 본 연구에서는 흑효모 유래 저분자 베타글루칸의 효용성을 확인하기 위해 A. pullulans 발효 배지로부터 얻은 베타글루칸을 수산화나트륨을 이용한 알칼리 가수분해로 가용성이 높은 저분자 베타글루칸으로 제조하였으며, 이것이 M1 및 M2 대식세포의 분극화에 미치는 영향을 조사하였다. RAW264.7 대식세포를 이용하여 lipopolysaccharide (LPS)와 interferon-γ (IFN-γ)로 자극하여 M1 대식세포로 분극화하였고, M2 대식세포로의 분극화는 interleukin-4 (IL-4)와 IL-13을 사용하였다. 각각의 분극화 과정에서 흑효모 유래 저분자 베타글루칸을 함께 처리한 결과, M1 대식세포의 마커인 CD86의 발현이 현저하게 감소하였으며, nitric oxide, IL-6, tumor necrosis factor-α와 같은 M1 대식세포의 마커 생성도 현저하게 감소함을 확인하였다. 또한, 흑효모 유래 저분자 베타글루칸의 처리가 M1 대식세포로의 분극화에 핵심적인 역할을 하는 전사인자인 signal transducer and activator of transcription (STAT)-1의 인산화를 억제하였다. 대조적으로, M2 대식세포의 마커인 IL-10은 IL-4와 IL-13로 자극한 RAW264.7 세포에서 현저하게 증가하였다. 이러한 결과는 흑효모 유래 저분자 베타글루칸이 대식세포의 M1/M2 분극화를 변화시켜 STAT-1의 하향 조절을 통해 항염증 활성을 나타내며, 염증성 질환의 잠재적인 치료제로서의 가능성을 시사한다.

Beta-glucan (β-glucan) extracted from the black yeast Aureobasidium pullulans (A. pullulans) is a natural products that is attracting attention due to its significant as a functional food and medicine. In this study, highly soluble low-molecular-weight β-glucan was obtained from an A. pullulans fermentation medium through alkaline hydrolysis using sodium hydroxide, and its effects on M1 and M2 macrophage polarization were investigated. RAW 264.7 macrophages were polarized to M1 through stimulation with lipopolysaccharide (LPS) and interferon-γ (IFN-γ), or to M2 with interleukin 4 (IL-4) and IL-13, in the presence of different concentrations of low-molecular-weight β-glucan extracted from the A. pullulans. Following the β-glucan treatment, the surface marker CD86 and the production of M1-type proinflammatory mediators were reduced. Additionally, phosphorylation of the signal transducer and activator of transcription (STAT)-1 increased in the LPS + IFN-γ-stimulated RAW264.7 cells, while the M2-associated IL-10 production significantly increased in IL-4 + IL-13-stimulated RAW264.7 cells. These findings indicate that low-molecular-weight β-glucan from A. pullulans can alter the M1/M2 polarization of macrophages, thereby playing an anti-inflammatory role via STAT-1 downregulation. This suggests its potential use as an effective immune modulator for attenuating the progression of inflammatory diseases.

The aim this study was to investigate the effectiveness and meaning of agro-healing programs for people with mental disabilites in vocational rehabilitation facilities. A mixed-methods approach was conducted, including a quantitative study with 12 participants diagnosed with mental disabilities and a qualitative study involving two staff members working at a vocational rehabilitation facilities located in Ganghwa-gun. The main results were as follows. First, the subjective perception of physical health among participants with mental disabilities, measured using a single-item 5-point likert scale, showed an increase after the program compared to the initial state, although the change was not statistically significant by Wilcoxon signed rank test. In contrast, subjective mental health perception assessed using the CES-D-10 and self-care awareness evaluated through a single-item 5-point likert scale were statistically significant positive improvements following the completion of the program. Secondly, opinions on future vocational rehabilitation-related agro-healing programs were high in the areas of plant resources and activities other than agriculture. Thirdly, the perceived effectiveness and meaning of agro-healing programs by staff members were analyzed through qualitative research methods. The findings revealed three main themes: "everyone returns to normal daily life," "improvement of health for individuals with mental disabilities," and "the path of life's challenges and explorations," along with 10 key sub-themes. Based on these results, practical and policy recommendations were proposed to integrate agriculture and welfare for the vocational rehabilitation of individuals with mental disabilities.

본 연구에서는 딥러닝 기반 영상 분석 기법인 You only look once(YOLO)를 활용하여 동적원심모형실험 상의 구조물 진동 변위를 측정하였다. 해당 기법의 적용성을 검증하기 위해 진동 가진기 실험을 선행으로 수행하였다. 그 결과 YOLO 기법으로 개발된 구조물 인식모델은 기존의 상용 트래킹 소프트웨어에 비해 우수한 안정성과 정확성을 보였다. 이후 원심모형실험에 이 기법을 적용하였고, 가속도계 이미지를 직접 인식하는 기법과 전처리 방식 중 가장 높은 정확도를 나타낸 Deep Image Enhancement 기법을 결합하여 평균 오차율 7.096%을 달성하였다. 또한 고속푸리에변환을 통해 유사한 주파수 특성이 도출되어 본 기법의 신뢰성을 추가로 입증하였다.

A deep learning-based image analysis approach, You Only Look Once (YOLO), was adopted to quantify the dynamic displacement of a structure in centrifuge model tests. To verify the method's effectiveness, a vibration excitation experiment was conducted prior to its application in the centrifugal model tests. The results demonstrated that the proposed YOLO-based model exhibited high stability and accuracy, outperforming conventional tracking software. In the centrifuge model tests, the approach that combined direct accelerometer measurements with preprocessing via a deep image enhancement technique achieved the highest accuracy, with an error rate of 7.096%. Additionally, fast Fourier transform analysis revealed comparable frequency characteristics, further validating the method's reliability.

Objective: This study examines the roles and support experiences of early childhood teachers in play-based learning through a phenomenological lens. It aims to explore how teachers perceive their roles and navigate challenges while facilitating play in early childhood education settings. Methods: This qualitative study employed a phenomenological approach, conducting in-depth, semi-structured interviews with four early childhood teachers. Participants were purposefully selected based on a minimum of three years of teaching experience in diverse early childhood settings. Data were collected over eight weeks and analyzed using Giorgi's phenomenological method. Results: The analysis yielded 74 statements, 21 sub-components, 11 components, and four major themes. The four major themes identified were "Types of Play," "Teacher's Role," "Limitations of Play Support," and "Teachers' Perception of Play." Conclusion/Implications: The findings highlight the critical role of teachers in fostering supportive play environments while balancing pedagogical goals with children's autonomy. Professional development programs emphasizing reflective practices, play facilitation skills, and emotional support strategies are essential for enhancing early childhood education outcomes.

복숭아혹진딧물(Myzus persicae Sulzer, Hemiptera: Aphididae)은 채소작물, 과수작물과 화훼작물에 발생하여 농업과 원예작물에 경제적 피해를 주는 주요 해충이다. 복숭아혹진딧물과 기주내부 미생물간의 상호작용을 이해하기 위해, 격리된 사육시설에서 약 2년간 누대사육된 복숭아 혹진딧물 내의 미생물 군집구조는 16S rRNA gene을 이용하여 분석하였다. 복숭아혹진딧물에 우점하는 미생물은 γ-proteobacteria에 속하는 Buchnera sp., Pseudomonas sp., Propionibacterium sp. 등이 주요 우점하는 것을 확인되었다. 미생물 군집크기분석은 정량 PCR을 통해 분석하였으며, 박테리아 군집의 크기가 곰팡이 군집보다 약 4,300배 큰 것을 확인하였다. 이러한 결과로부터 복숭아혹진딧물 내부에는 γ-proteobacteria에 속하는 Buchnera sp.가 기주와 매우 밀접한 상호관계가가 이루어진다고 판단되어진다. 본 연구의 결과는 복숭아혹진딧물과 기주내부 미생물간의 상호작용을 이해하는데 기초자료로 제공될 수 있을 것으로 기대된다.

The green peach aphid (Myzus persicae Sulzer, Hemiptera: Aphididae) is a significant agricultural pest, inflicting economic loss on vegetable crops, fruit trees, and ornamental plants. To elucidate the interactions between M. persicae and its endosymbiotic microbial communities, the microbiota of aphids maintained in isolated rearing conditions for approximately two years were analyzed using 16S rRNA gene sequencing. The dominant microbial taxa identified included γ-proteobacteria, such as Buchnera sp., Pseudomonas sp., and Propionibacterium sp. Quantitative PCR revealed that the bacterial community size was approximately 4,300 times greater than the fungal community size. Among these, Buchnera sp., a γ-proteobacterium, exhibited a highly intimate symbiotic relationship with the host aphid. These findings contribute foundational knowledge toward understanding the interactinos between M. persicae and its associated microbial communities, with implications for the management of this pest species.

In this study, the feasibility of utilizing waste sludge generated from an S-water purification facility that treats coal mine wastewater contaminated with Fe and Mn as an adsorbent for arsenic removal from contaminated water and the arsenic adsorption mechanism were evaluated. The physical, chemical, and structural characteristics of S-CMDS (coal mine drainage sludge) were analyzed, revealing a mesoporous structure and a large specific surface area of 242 m²/g. The particle surface exhibited a positive charge, with a point of zero charge (pH_zpc) of 8.78. The total Fe content was measured as 3.175 g/kg, and SEM-EDS analysis confirmed a high Fe to O ratio. XRD analysis revealed the presence of peaks of magnesium-bearing calcite, but no peaks of iron-hydroxide minerals were observed, indicating that S-CMDS consists of amorphous iron hydroxide. Dynamic adsorption experiments demonstrated that the pseudo-second-order kinetic model accurately described the adsorption behavior, with equilibrium adsorption capacities (q_e) for As(III) and As(V) of 5.204 mg/g and 5.271 mg/g, respectively, at pH 3. However, the q_e values decreased significantly at pH values above pH 9, confirming the strong influence of pH on the adsorption performance. The calculated adsorption bond energies for As(III) and As(V) were 10.907 kJ/mol and 12.671 kJ/mol, respectively, suggesting that ion exchange is the dominant adsorption mechanism. Furthermore, analysis using the intraparticle diffusion model indicated that intraparticle diffusion is the rate-limiting step in the arsenic adsorption process. Based on these results, S-CMDS demonstrates strong potential as an effective adsorbent for the removal of anionic arsenic and is expected to serve as an eco-friendly and efficient material for the treatment of arsenic-contaminated water.

활성산소종은 미토콘드리아 대사활동을 통해 생성되는 반응성이 강한 산소 함유 분자로서 특히 간에서 복잡한 세포 분자와 반응하여 산화적 스트레스를 유발하는 물질이다(Choi et al., 2012). 에탄올 대사 과정에서는 ROS가 생성되어 산화스트레스를 유발하며, 과도한 에탄올은 ROS를 제거하는 항산화 시스템을 억제함으로써 지방, 단백질 또는 DNA와 같은 생체 분자에 산화 손상을 유발하여 세포를 손상시킨다(Albano, 2006; Koch et al., 2004). 본 연구에서는 오리나무 잎 추출물(AJL)에서 알코올(EtOH)로 유발된 산화적 스트레스에 대한 간세포 손상의 보호 효과를 평가하기 위해 HepG2/2E1 세포에서 EtOH와 AJL 및 대조군silymarin을 각각 처리한 후 세포생존율을 평가하고 세포독성이 없는 범위를 설정하여 활성산소종 생성량, 항산화 관련 지표(DPPH, ABTS, MDA, GSH) 및 간 손상 지표(AST 및 ALT)를 측정하였다. 알코올에 대한 간 보호 효과에서는 알코올만 처리한 군의 세포생존율이 53.8%로 감소하였으나 AJL을 함께 처리 군에서 세포생존율이 85.1%의 유의적인 증가로 간 보호효과를 확인하였다. 다음으로 AJL에서 세포 내 ROS 생성 억제능은 에탄올 처리군에서 정상군에 비해 유의적으로 ROS 생성이 증가하였으나 AJL 농도별 처리시 정상군 수준으로 ROS 생성을 현저하게 감소시켰다. MDA 함량은 에탄올 처리군에서 무처리군에 비해 유의적으로 증가하였으나 AJL 처리시 농도 의존적으로 에탄올 처리군에 비해 유의적인 차이를 보이며 MDA 함량을 감소시켰다. GSH 함량은 에탄올 처리군에서 정상군보다 유의적으로 함량이 감소하였으나 AJL 농도별 처리군 모두 에탄올 처리군에 비해 GSH 함량을 증가시켰다. 또한 간손상 지표로 활용되는 AST 및 ALT 활성 변화 확인 시, 에탄올 처리에 따른 간세포 손상군에 비하여 AJL 처리군에서는 농도 의존적으로 현저하게 감소시켰으며, 대조군silymarin과 비교하여 유사한 감소된 결과를 관찰하였다. 이상의 결과로부터 종합해 볼때AJL은 간손상에 대한 보호 및 간손상 억제 가능성을 기대할 수 있으며, 간기능 개선 효과에 대한 건강기능식품개발의 기초자료로서 활용될 수 있을 것이라 사료된다.

Alcoholic liver disease (ALD) is a significant risk factor in the global disease burden. The stem bark of the Betulaceae plant Alnus japonica, which is indigenous to Korea, has been used as a popular folk medicine for hepatitis and cancer. However, the preventive effect of Alnus japonica leaf extracts on alcohol-related liver damage has not been investigated. The objective of this study was to investigate the hepatoprotective effects of the extracts of Alnus japonica leaf (AJL) against ethanol-induced liver damage in HepG2/2E1 cells. Treatment with AJL significantly prevented ethanol-induced cytotoxicity in HepG2/2E1 cells by reducing the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). This protective effect was likely associated with antioxidant potential of AJL, as evidenced by the attenuation of reactive oxygen species (ROS) and malondialdehyde (MDA) production and restoration of the depleted glutathione (GSH) levels in ethanol-induced HepG2/2E1 cells. Our findings suggest that FCC might be considered as a useful agent in the prevention of liver damage induced by oxidative stress by increasing the antioxidant defense mechanism.

Rapid, early, accurate detection and identification of the various pathogenic agents associated with the development of biological weapons is critical in preventing loss of life and limiting the impact of these organisms when used against civilian or military targets. The aim of this study was to produce a system for the simple, rapid, accurate and simultaneous detection and identification of Ricin, Botulinum toxin B and Staphylococcal enterotoxin B as a proof of principle for developing field appropriate reverse transcription loop-mediated isothermal amplification systems for the accurate identification of potential biological threats. These systems were designed to facilitate the identification of potential threats even in remote or resource-limited locations.

올리브(Olea europaea L.)는 올리브유를 생산하는 중요한 영양학적 및 경제적 이익을 제공하는 매우 중요한 작물이다. 우리나라는 2009년 제주도에 올리브 5품종을 도입하였으며, 올리브 재배시험은 2012년 제주도에서 시작되었다. 본 연구는 올리브 4개 품종('Frantoio', 'Koroneiki', 'Leccino', 'Verdale')에 대한 수체생육과 과실특성을 평가하고 생육기간에 따른 기상을 분석하여 재배적지를 평가하고자 하였다. 올리브 수체생육 중 수폭은 서귀포시 식재 올리브가 제주시 식재 올리브 보다 약 107~121%정도 양호하였다. 또한, 올리브 개화 후 6일간의 일평균습도는 제주시 82.3%, 서귀포시 92.4%로 확인되었으며, 서귀포시가 제주시보다 10.2~19.2% 높은 것으로 확인되었다. 본 연구를 통해 수체생육은 제주시보다 서귀포시가 좀 더 유리하였으며, 과실의 생산량과 과실크기는 제주시가 서귀포시보다 우세하였다. 또한, 개화기의 대기습도, 강수량, 강우일수와 같은 기상환경이 올리브 과실 생산에 매우 큰 영향을 줄 수 있다는 것을 관찰하였다. 본 연구의 결과는 한국의 올리브 재배적지를 선정하는데 기초자료로 활용될 것으로 기대한다.

Olive (Olea europaea L.) is a crucial crop that provides significant nutritional and economic benefits, primarily through olive oil production. Korea introduced five cultivars of olives in Jeju Island in 2009. In addition, olive cultivation field tests began on Jeju Island in 2012. This study investigated the growth and fruit characteristics of four olive cultivars ('Frantoio', Koroneiki', 'Leccino', and 'Verdale') planted in Jeju-si and Seogwipo-si. Also, it was intended to evaluate the suitability of olive plantations by analyzing the climatic conditions during the growth period. The wide diameter growth of olive planted in Seogwipo-si was approximately 107 to 121% better than those planted in Jeju-si. Also, the average daily humidity for six days after flowering of Jeju-si and Seogwipo-si was 82.3% and 92.4%, respectively. The difference in daily average humidity was 10.2 to 19.2% higher in Seogwipo-si than in Jeju-si. This study found that Seogwipo-si is more favorable for tree growth, while Jeju-si demonstrated superiority in fruit yield and size. Also, it has been observed that weather conditions, including air humidity, rainfall, and the number of rainy days during the flowering period, significantly impact olive fruit production. These results are expected to serve as foundational data for selecting optimal olive cultivation sites in South Korea.

복숭아혹진딧물의 포식기생자인 콜레마니진디벌의 기능반응을 온도별(15, 20, 25, 30℃) 복숭아혹진딧물 밀도를 달리하여(2, 4, 8, 16, 32, 64, 128마리) 평가하였다. 모든 온도 처리에서 콜레마니진디벌은 제 3 유형의 기능반응을 보였다. 30℃에서 공격률(0.0043 day^-1)은 높았지만 처리시간(0.9268 day)은 다른 온도보다 길었다. 추정된 처리시간은 온도가 15℃에서 25℃로 증가할수록 감소하였다. 콜레마니진디벌의 24시간동안 최대 공격률은 15, 20, 25, 30℃에서 29.1, 31.9, 59.3, 25.9이었다. 온도처리별 복숭아혹진딧물 기생률은 유사한 특징을 나타내었다. 가장 높은 기생률은 15, 20, 25, 30℃에서 복숭아혹진딧물 32, 32, 16, 32마리에 대해 0.52, 0.54, 0.57, 0.40이었으며 25℃에서 콜레마니진디벌의 기생력이 가장 높게 나타났다. 본 연구는 복숭아혹진딧물의 생물적 방제원으로서 콜레마니진디벌의 효율성을 여러 온도조건에서 평가하였다.

In this study, we evaluated the functional response of Aphidius colemani Viereck (Hymenoptera: Braconidae), a parasitoid of Myzus persicae (Sulzer) (Hemiptera: Aphididae), at different temperatures (15, 20, 25, 30℃) and varying aphid densities (2, 4, 8, 16, 32, 64, 128 individuals per leaf). At all temperature treatments, A. colemani exhibited a Type III functional response. The attack rate was high at 30℃ (0.0043 day^-1), but the handling time (0.9268 day) was longer compared to other temperatures. The estimated handling times decreased as the temperature increased from 15℃ to 25℃. The maximum attack rates of A. colemani over 24 hours were 29.1, 31.9, 59.3, and 25.9 at 15, 20, 25, and 30℃, respectively. The parasitism rates of M. persicae under different temperature treatments showed similar characteristics. The highest parasitism rates were observed at aphid densities of 32, 32, 16, and 32 individuals for 15, 20, 25, and 30℃, with values of 0.52, 0.54, 0.57, and 0.40, respectively; the parasitism efficiency of A. colemani was highest at 25℃. This study evaluates the efficiency of A. colemani as a biological control agent for M. persicae under various temperature conditions.

Microbial forensics is a scientific discipline for analyzing evidence related to biological crimes by identifying the origin of microorganisms. Multiple locus variable number tandem repeat analysis(MLVA) is one of the microbiological analysis methods used to specify subtypes within a species based on the number of tandem repeat in the genome, and advances in next generation sequencing(NGS) technology have enabled in silico anlysis of full-length whole genome sequences. In this paper, we analyzed unknown samples provided by Robert Koch Institute(RKI) through The United Nations Secretary-General's Mechanism(UNSGM)'s external quality assessment exercise(EQAE) project, which we officially participated in 2023. We confirmed that the 3 unknown samples were B. anthracis through nucleic acid isolation and genetic sequence analysis studies. MLVA results on 32 loci of B. anthracis were analysed by using genome sequences obtained from NGS(NextSeq and MinION) and Sanger sequencing. The MLVA typing using short-reads based NGS platform(NextSeq) showed a high probability of causing assembly error when a size of the tandem repeats was grater than 200 bp, while long-reads based NGS platform(MinION) showed higher accuracy than NextSeq, although insertion and deletion was observed. We also showed hybrid assembly can correct most indel error caused by MinION. Based on the MLVA results, genetic identification was performed compared to the 2,975 published MLVA databases of B. anthracis, and MLVA results of 10 strains were identical with 3 unkonwn samples. As a result of whole genome alignment of the 10 strains and 3 unknown samples, all samples were identified as B. anthracis strain A4564 which is associated with injectional anthrax isolates in heroin users.

The COVID-19 pandemic is not over despite the emergency use authorization as can see recent COVID-19 daily confirmed cases. The viruses are not only difficult to diagnose and treat due to random mutations, but also pose threat human being because they have the potential to be exploited as biochemical weapons by genetic manipulation. Therefore, it is inevitable to the rapid antibody-based therapeutic platform to quickly respond to future pandemics by new/re-emerging viruses. Although numerous researches have been conducted for the fast development of antibody-based therapeutics, it is sometimes hard to respond rapidly to new viruses because of complicated expression or purification processes for antibody production. In this study, a novel rapid antibody-based therapeutic platform using single B cell sorting method and mRNA-antibody. High immunogenicity was caused to produce antibodies in vivo through mRNA-antigen inoculation. Subsequently, antigen-specific antibody candidates were selected and obtained using isolation of B cells containing antibody at the single cell level. Using the antibody-based therapeutic platform system in this study, it was confirmed that novel antigen-specific antibodies could be obtained in about 40 days, and suggested that the possibility of rapid response to new variant viruses.