Earticle

현재 위치 Home
간행물 내 원문 검색
많이 이용된 논문

Issues

Reproductive & developmental biology

간행물 정보
  • 자료유형
    학술지
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 간기
    계간
  • 수록기간
    1977 ~ 2018
  • 주제분류
    농수해양 > 축산학
  • 십진분류
    KDC 527 DDC 636
Volume 41 No 4 (1건)
No
1

Antioxidant Effect of Alpha-Linolenic Acid during In Vitro Maturation in Porcine Oocytes

Ji-Eun Lee, Yong Hwangbo, Hwa-Young Kim, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park

한국동물생명공학회(구 한국동물번식학회) Reproductive & developmental biology Volume 41 No 4 2017.11 pp.65-70

※ 기관로그인 시 무료 이용이 가능합니다.

4,000원

Alpha-linolenic acid (ALA) is one of n-3 polyunsaturated fatty acids and found mainly in the chloroplasts. Many studies have been reported that intracellular reactive oxygen species (ROS) in mammalian oocytes were reduced by supplementation of ALA in in vitro maturation (IVM) medium. Based on these reports, we expected that ALA acts as an antioxidant during IVM of porcine oocytes. Therefore, the objective of this study was to investigate the antioxidant effect of ALA supplementation during IVM in porcine oocytes. The cumulus-oocyte complexes (COCs) were incubated in IVM medium containing 200 μM H2O2 or H2O2 with 50 μM ALA for 44 h. Nuclear maturation stage of oocytes was evaluated using aceto-orcein method. For measurement of oxidative stress state, intracellular ROS and glutathione (GSH) levels were measured using carboxy-DCFDA and cell tracker red, respectively. In results, oocytes in metaphase-II (MII) stage development was significantly reduced in H2O2 group compared to non- treated control group (61.84±1.42% and 80.00%, respectively; p<0.05) and it was slightly recovered by treatment of ALA (69.76±1.67%; p<0.05). The intracellular GSH levels was decreased in H2O2 groups compared with control groups, but it was enhanced by ALA treatment (p<0.05). On the contrary, H2O2 treatment increased intracellular ROS level in oocytes and H2O2-induced ROS was decreased by treatment of ALA (p<0.05). Our findings suggested that ALA treatment under oxidative stress condition improve oocyte maturation via elevated GSH and reduced ROS levels in oocytes. Therefore, these results suggest that ALA have an antioxidative ability and it could be used as antioxidant in in vitro production system of porcine embryo.

 

0개의 논문이 장바구니에 담겼습니다.

구매 불가 논문

    선택하신 파일을 압축중입니다.
    잠시만 기다려 주십시오.

    다운로드 불가 논문
    페이지 저장
    소속기관 조회
    이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
    기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.