Earticle

초록

영어

Alpha-linolenic acid (ALA) is one of n-3 polyunsaturated fatty acids and found mainly in the chloroplasts. Many studies have been reported that intracellular reactive oxygen species (ROS) in mammalian oocytes were reduced by supplementation of ALA in in vitro maturation (IVM) medium. Based on these reports, we expected that ALA acts as an antioxidant during IVM of porcine oocytes. Therefore, the objective of this study was to investigate the antioxidant effect of ALA supplementation during IVM in porcine oocytes. The cumulus-oocyte complexes (COCs) were incubated in IVM medium containing 200 μM H2O2 or H2O2 with 50 μM ALA for 44 h. Nuclear maturation stage of oocytes was evaluated using aceto-orcein method. For measurement of oxidative stress state, intracellular ROS and glutathione (GSH) levels were measured using carboxy-DCFDA and cell tracker red, respectively. In results, oocytes in metaphase-II (MII) stage development was significantly reduced in H2O2 group compared to non- treated control group (61.84±1.42% and 80.00%, respectively; p<0.05) and it was slightly recovered by treatment of ALA (69.76±1.67%; p<0.05). The intracellular GSH levels was decreased in H2O2 groups compared with control groups, but it was enhanced by ALA treatment (p<0.05). On the contrary, H2O2 treatment increased intracellular ROS level in oocytes and H2O2-induced ROS was decreased by treatment of ALA (p<0.05). Our findings suggested that ALA treatment under oxidative stress condition improve oocyte maturation via elevated GSH and reduced ROS levels in oocytes. Therefore, these results suggest that ALA have an antioxidative ability and it could be used as antioxidant in in vitro production system of porcine embryo.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Oocytes Collection and In Vitro Maturation
  Measurement of Intracellular GSH and ROS Levels
  Evaluation of Nuclear Maturation
  Statistical Analysis
 RESULTS
  Changes of Intracellular ROS and GSH Level by ALA under Oxidative Stress
  Antioxidant Effect of ALA on Nuclear Maturation of Porcine Oocytes
 DISCUSSION
 REFERENCES

키워드

저자

• Ji-Eun Lee [ College of Animal Life Sciences, Kangwon National University, Chunchoen 24341, Republic of Korea ]
• Yong Hwangbo [ College of Animal Life Sciences, Kangwon National University, Chunchoen 24341, Republic of Korea ]
• Hwa-Young Kim [ College of Animal Life Sciences, Kangwon National University, Chunchoen 24341, Republic of Korea ]
• Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University, Chunchoen 24341, Republic of Korea ]
• Boo-Keun Yang [ College of Animal Life Sciences, Kangwon National University, Chunchoen 24341, Republic of Korea ]
• Choon-Keun Park [ College of Animal Life Sciences, Kangwon National University, Chunchoen 24341, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

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• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636