Earticle

현재 위치 Home
간행물 내 원문 검색
많이 이용된 논문

Issues

Reproductive & developmental biology

간행물 정보
  • 자료유형
    학술지
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 간기
    계간
  • 수록기간
    1977 ~ 2018
  • 주제분류
    농수해양 > 축산학
  • 십진분류
    KDC 527 DDC 636
Volume 40 No 3 (1건)
No
1

돼지 정자의 동결보존 시 α-Linolenic Acid의 효과

이원희, 황보용, 이상희, 양진우, 김화영, 이유림, 박지은, 정희태, 양부근, 박춘근

한국동물생명공학회(구 한국동물번식학회) Reproductive & developmental biology Volume 40 No 3 2016.08 pp.27-31

※ 기관로그인 시 무료 이용이 가능합니다.

4,000원

The aim of this study was to evaluate effect of α-linolenic acid (ALA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved in 20% egg yolk freezing extender containing ALA (0, 3, 5, and 10 ng/mL) with 0.05% ethanol. The frozen-boar spermatozoa were thawed at 37.5℃ for 45 sec in water-bath. The spermatozoa samples were evaluated the plasma membrane integrity, acrosome reaction, and mitochondrial integrity using flow cytometry. In results, population of live sperm with intact plasma membrane was significantly higher in control and 3 ng/mL ALA treatment group than ethanol group (p<0.05). In contract, dying sperms were higher in ethanol group than 3 ng/mL ALA treatment (p<0.05). Acrosomal membrane damage in all sperm population was reduced in 3 ng/mL ALA groups compared with ethanol treatment (p<0.05). However, acrosome damage in live sperm population was no significant difference among the all treatment groups. Mitochondrial integrity was not influenced by ALA treatments in both of live and all sperm population. In conclusion, this results show that supplement of ALA during the cryopreservation process could reduce the membrane damages including plasma and acrosomal membrane, whereas ALA did not influence to mitochondria in boar spermatozoa. Therefore, these results suggest that ALA can protect against the membrane damage derived cryo-stress, and cryopreservation efficiency of boar semen would be improved by use of ALA.

 

0개의 논문이 장바구니에 담겼습니다.

구매 불가 논문

    선택하신 파일을 압축중입니다.
    잠시만 기다려 주십시오.

    다운로드 불가 논문
    페이지 저장
    소속기관 조회
    이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
    기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.