Earticle

This paper explores the linguistic specificity of English and Korean by comparing the formal realizations of specificity. The indefinite articles in English and zero articles in Korean are argued not to indicate the specificity features of the noun phrase[NP] concerned, with these features dependent partially on the contexts of the sentences and partly on additional lexical markers and word orderings of the arguments under the principle of communicative dynamism. Secondly, the two aspects of specificity effects are compared between the two languages. The first effect concerns the gradience of specificity when supplementary elements like certain/specific in English and etten/-ul in Korean are added to noun phrases. The supplementary elements in English ensure the specificity of the NPs, while these elements in Korean increase the probability of specificity, but do not guarantee it. The second effect is that the notion of specificity serves as a constraint on movement and co-reference. In English the specificity constraint for movement applies to the wh-movement and complex NP constraint for relativization, while it does not apply in the Korean language. It is argued that the specificity requirements for co-reference applies to long-distance co-reference of personal pronouns in English, while they apply to the reflexive pronoun caki and casin distinction in Korean.

One account of English L2 learners’ errors in the use of articles is based on the assumption that they are influenced by the specificity of the reference, which is a non-target semantic feature, as contrasted with the target feature, definiteness. Another problem that has been increasingly recognized, and yet treated independently of the specificity-driven errors, is L2 learners’ tendency to use articles excessively in the contexts for a zero form. The present study aims to seek for a connection between these two problems by reexamining the intervention effect of specificity on Korean EFL learners’ substitution errors of the definite and indefinite articles, together with the patterns of their excessive use of articles in zero-form contexts. Data were collected from three proficiency groups of high school students (N = 102) with a forced-choice elicitation task comprising 40 questions across 8 distinct combinations of three semantic features: definiteness, specificity, and plurality. The results indicate that Korean students are only partially under the influence of specificity; their responses did not fluctuate consistently according to the value of specificity in relation to that of definiteness. That they also make omission errors frequently suggests that their awareness of the zero article affects their decision in the first place before they choose between a and the . Furthermore, Korean students tend to use the for indefinite singulars and generic plurals, while overusing the zero article for non-generic definite plurals. These findings imply that definiteness is a notion that requires learning rather than resetting and that its acquisition correlates more closely with the usage of the zero article than with the specificity ambiguities of the indefinite article.

실증적인 연구들에 의하면 고객회사(client firms)와 서비스 제공업자(vendor) 간의 파트너십의 질과 범위가 정보시스템 아웃소싱의 성공적인 구현에 결정적인 역할을 하는 것으로 알려졌다. 본 연구는 이러한 정보시스템 아웃소싱 구현에 있어서 파트너십의 역할을 좀 더 심층적으로 분석하려는 노력의 일환이다. 본 연구에서의 기본 명제는 파트너십 특성들의 아웃소싱 구현에의 효과는 아웃소싱 업무의 특성(task characteristics)과 같은 상황적 요인에 의해 영향을 받을 수 있다는 것이다. 구체적으로 본 연구에서는 자산특이성(asset specificity)과 파트너십 특성간의 상호효과(interaction effect)에 중점을 두고 조사를 하게되는데, 파트너십은 관계적거래 특성(relational exchange characteristics)에 의해 구체화되었다. 207개의 미국 회사의 아웃소싱 관계를 대상으로 한 설문조사를 기반으로 한 결과에 의하면, 전반적으로 비관계적거래(discrete exchange)보다 관계적거래에 기반을 두고 아웃소싱 관계를 운영하는 것이 아웃소싱의 성공적 구현을 위하여 필수적인 것으로 보인다. 한편 상호효과를 조사하기 위해서는 MRA와 하위그룹분석(subgroup analysis)을 시행하였는데, 자산특이성과 일부 관계적거래 특성들간에 상호효과가 유의성이 있음을 발견하였다. 발견된 상호효과를 근거로 상황 변수를 고려하지 않은 연구 결과 중 확실하지 않은 결과(inconclusive results)에 대한 해석을 시도하였다.

디지털 환경에서 소비자는 구매 이전 단계에서 검색을 통해 정보를 탐색하며, 이 과정에서 나타나는 검색 키워드는 소비자의 의사결정 상태를 반영하는 중요한 행동적 지표로 볼 수 있다. 이에 본 연구는 구매 의사결정 여정 단계와 제품의 혁신성이 검색 키워드의 구체성에 미치는 영향을 실험을 통해 검증하였다. 연구 결과, 소비자는 의사결정 여정이 진행됨에 따라 점차 구체적인 정보를 탐색하는 경향을 보였다. 그러나 이러한 경향은 제품의 혁신성에 따라 상이하게 나타났다. 구체적으로, 혁신제품의 경우 여정이 진행됨에도 불구하고 검색 키워드의 구체성이 상대적으로 낮은 수준에 머무르는 반면, 비혁신제품은 초기 단계부터 비교적 높은 수준의 구체적 탐색이 이루어지는 것으로 나타났다. 본 연구는 제품의 혁신성이 소비자의 정보탐색 방식에 영향을 미치며, 동일한 의사결정 여정 단계에서도 검색 행동이 달라질 수 있음을 보여준다. 이를 통해 소비자 구매 의사결정 여정에 대한 이해를 확장하고, 검색 기반 환경에서의 마케팅 전략 수립을 위한 실무적 시사점을 제시하였다.

In a digital environment, consumers actively search for information prior to purchase, and the search keywords generated in this process can serve as important behavioral indicators reflecting their decision-making state. This study examines the effects of decision journey stages and product innovativeness on the specificity of search keywords through an experimental design. The results show that consumers tend to search for more specific information as they progress along the decision journey. However, this pattern varies depending on the level of product innovativeness. Specifically, for innovative products, the specificity of search keywords remains relatively low even as the decision journey progresses, whereas for non-innovative products, consumers exhibit relatively high levels of specific search behavior from the early stages. These findings suggest that product innovativeness influences consumers’ information search patterns and that search behavior may differ even at the same stage of the decision journey. This study extends the understanding of the consumer decision journey and provides practical implications for developing marketing strategies in search-based environments.

본 연구는 긍정 정서 반응이 우울 증상에 미치는 영향을 자서전적 기억 특정성이 조절하는지를 검증하였다. 이를 위해 260명의 대학생을 대상으로, 한국판 긍정 정서 반응 척도(K-RPA), 자서전적 기억 검사(AMT), 및 통합적 한 국판 CES-D 척도를 사용하여 설문을 실시하였다. 그 결과, 긍정 정서 반응의 긍정 반추 요인과 우울 증상의 관계 에서 자서전적 기억 특정성의 조절효과가 유의미하였다. 다시 말해, 자서전적 기억 특정성이 중간과 높은 집단에 서는 긍정 반추의 증가에 따라 우울 증상이 감소하였다. 하지만 자서전적 기억 특정성이 낮은 집단에서는 긍정 반 추가 증가하여도 우울 증상이 감소하지 않았다. 또한, 긍정 정서 반응의 가라앉히기 요인은 우울 증상에 정적 영 향을 미쳤다. 이러한 결과를 바탕으로 본 연구의 의의와 제한점 및 추후 연구의 방향에 대하여 논의하였다.

This study examined the moderating effect of autobiographical memory specificity on the relationship between responses to positive affect and depressive symptoms. Two hundred and sixty undergraduate students completed the Korean-Responses to Positive Affect Questionnaire(K-RPA), the Autobiographical Memory Test(AMT), and the Center for Epidemiological Studies-Depression Scale(CES-D). The results indicated that autobio- graphical memory specificity moderated the relationship between positive rumination and depressive symptoms. That is, in groups with moderate and high autobiographical memory specificity, the depressive symptoms decreased with the increase of positive rumination. However, in groups with low autobiographical memory specificity, the depressive sym- ptoms did not decrease even as positive rumination increased. Dampening had a significant positive effect on depressive symptoms. Based on the results, the implications and limitat- ions of this study were discussed.

e-커머스 플랫폼 문헌에서 유효한 관점 중 하나는 정보 비대칭 상황에서의 판매자 신호전략이다. 본 연구에서는 판매자의 신호전략이 소비자의 의사결정에 미치는 영향을 체계적으로 탐색하기 위해 신호이론과 쇼핑목표이론을 기반으로 연구모델을 구성하였다. 구체적으로 소비자의 쇼핑 목표에 따라 판매자가 제공하는 신호 효과(i.e., 평판, 온라인 구전 비율, 가격)에 차이가 있는지 분석하였다. 실증분석을 위해서는 대표적인 e-커머스 플랫폼인 아마존을 대상으로 26,246개의 데이터를 수집하여 가우시안 코플라 방법을 활용하였다. 분석결과, 판매자가 제공하는 신호는 수익에 긍정적인 영향을 미치는 것으로 나타났으며, 이러한 효과는 소비자의 쇼핑목표에 따라 조절되는 것으로 분석되었다. 본 연구는 쇼핑목표이론을 기반으로 소비자가 입력하는 키워드에 따라 판매자의 신호전략 효과에 차이가 있다는 점을 발견함으로써 신호이론 및 전자상거래 문헌에 기여하였다.

One of the valid perspectives in the e-commerce platform literature is the seller signaling strategy in the information asymmetry situation. In this study, a research model was constructed based on signaling theory and shopping goal theory to systematically explore the effects of a seller's signaling strategy on consumer decision-making. Specifically, the study examined whether the signaling effects (i.e., reputation, electronic word-of-mouth, price) provided by the seller differed based on consumers' shopping goals. For the empirical analysis, the Gaussian Copula method was employed, utilizing 26,246 data collected from Amazon, a leading e-commerce platform. The analysis revealed that the signals provided by the seller positively impacted sales, and this effect was moderated by consumers' shopping goals. Drawing on shopping goal theory, this study contributes to signaling theory and e-commerce literature by discovering differences in the effectiveness of a seller's signaling strategy based on the keywords input by consumers.

본 연구는 거래비용이론을 경험재 선택에 적용하여 경험재의 거래특성과 거래비용에 대해 살펴보는 것을 목적으로 하였다. 경험재의 특성을 반영하여 경험특유성, 거래불확실성, 개인불확실성을 제안하고 이 변수들이 거래 비용에 주는 영향, 거래비용이 선택의도에 주는 영향을 가정하였다. 경험재 선택에 관한 207부의 데이터를 분석한 결과는 다음과 같다. 첫째, 경험특유성(장소특유성, 물리적장비특유성, 지식기술특유성, 시간특유성), 거래불확실성 (제품불확실성, 과정불확실성), 개인불확실성(선호불확실성, 상황불확실성)은 거래비용에 모두 긍정적 영향을 주는 것으로 나타났다. 둘째, 거래비용(탐색비용, 비교비용, 검사비용, 협상비용, 주문과지불비용, 전달비용)은 해당 경험 재의 선택의도에 부정적 영향을 주는 것으로 나타났다. 본 연구의 결과는 거래비용의 증가가 경험재의 선택 가능성 을 줄일 수 있고, 거래비용을 감소시키기 위해 경험특유성과 거래불확실성, 개인불확실성의 전략적 고려가 필요함을 보여주고 있다. 또한 거래와 비용적 관점에서의 접근이 부족했던 경험재 연구를 보완함으로써 이론적인 기여를 하고 있다.

The purpose of this study is to investigate the effect of transaction characteristics on transaction cost and choice intention by applying transaction cost theory to experiential product. Experience-specificity, transaction uncertainty, and personal uncertainty are proposed to reflect the characteristics of experiential products, and the effects of these variables on transaction costs and transaction costs are assumed to have an influence on the choice intention. The results of this study are summarized as follows. First, experience-specificity(site, physical equipment, knowledge·skill, temporal), transactional uncertainty(product-, process-), personal uncertainty (preference-, and situation-) have a significant positive effect on transaction cost. Second, transaction costs (search, comparison, examination, negotiation, payment, delivery) have a significant negative effect on the choice intention of the experiential product. The results of this study show that the increase of transaction costs can reduce the choice of experiential products and the strategic consideration of experience specificity, transaction uncertainty and individual uncertainty are required to reduce transaction costs. In addition, experiential products lacked access from a transactional and cost-based point of view, and this study contributes theoretically by compensating for the lack.

Park, Kee-Tae. “The Semantic-Pragmatic Analysis in the Existential There-Construction: focused on the Specificity Effect.” Studies in English Language & Literature 41.3 (2015): 135-152. There are some issues on inconsistent phenomena between practical language use and grammatical theory within the existential there-construction in English. Therefore, in this study, the aim is to point out the unsettled issues of "the definiteness effect" claiming that all associate NPs must be as indefinite expressions in many studies so far, and to prove that, as an alternative to settle down the issues, the theoretical adequacy of "the specificity effect" in Enç(1991) is more acceptable than that of the definiteness effect within the existential there-construction in English. In various comments, it has been broadly known that the definiteness effect prevents definite expressions from being as associate NPs after the predicative verb. In order to review several previous issues with the above remark from semantic-pragmatic perspectives, the fact that the definiteness effect may have the pragmatic nature will be discussed. With respect to this point, introducing “the specificity effect” which is counter to the definiteness effect, I discuss that all associate NPs must be interpreted as new information regardless of their definiteness. Thereby, I also discuss that the definite associates are reinterpreted, which is an inherent function different from that of the indefinite associates. In other words, the definite associates have the dual meanings (the dual functions) at the time of utterance. Thus, as one way to settle down the previous issues suggested in the existential construction with the definiteness effect, the role of "the specificity effect" must be very important to prove its theoretical pragmatic adequacy in the existential there-construction which asserts the existence of the postverbal entity of NP. (Konyang University)

During the last decade enzyme reaction in organic solvent has been studied to show that specificity in buffer is different from that in organic solvent. The specificity of restriction enzyme was effected by various factors such as ionic strength, salt organic solvent and temperature. In this study, restriction enzyme PvuII which is used most frequently in genetic engineering and the substrate was vector pGEM3 whose sequence was already known were used. As a result the recognition sequence site was changed in the presence of organic solvents whose Log P are -1.5∼0. Their specificities were contrast with activities were contrasted. Specificities were not changed in organic solvent easily in inactivating enzyme. We think that the enzyme recognition site was not changed randomly but by preferential order. A recombinant vector which does not contain typical cleavage site CAG↓CTG was cleaved in 20% ethanol solution. This result might show that restriction enzyme could be used to cleave at unusual sites by changing the reaction conditions.

In this paper, I propose that the specificity effect is able to improve or override weak crossover (WCO) phenomena, since [+specific] features associated with D-linking elements overtly move in wh-questions. These crossover configurations have been described in terms of syntactic factors. Postal (1971, 1993) and Wasow (1979) both refer to these kinds of crossover phenomena. Strong Crossover (SCO) violates the crossover principle or Binding Condition C. In contrast, WCO phenomena have remained marginal. The local constraints are not available to explain the variation associated with discourse connectivity, which includes WCO phenomena, or to capture pragmatic variation in context. From the perspective of semantic and pragmatic approaches, I suggest that specificity related to discourse context is able to explain the degree of the grammatical acceptability of wh-phrases in WCO configuration.

One account of English L2 learners' errors in the use of articles is based on the assumption that they are influenced by the specificity of the referent. Another problem that has been increasingly recognized, and yet treated independently of the alleged specificity-driven errors, is L2 learners' tendency to use articles excessively in the contexts for a zero form. The present study aims to seek for a connection between these two problems by retesting the intervention effect of specificity on Korean EFL learners' substitution errors of the definite and indefinite articles along with the patterns of their excessive use of articles in zero-form contexts. Data were collected from three proficiency groups of high school students (N = 102) with a forced-choice elicitation task comprising 40 questions across 8 distinct combinations of three semantic features: definiteness, specificity, and plurality. The analysis of their accuracy rates and error distributions indicate that they are only partially under the influence of specificity; their responses did not fluctuate consistently according to the value of specificity in relation to that of definiteness, which implies that definiteness is a grammatical feature that requires learning rather than resetting and that its learnability issue correlates more closely with the usage of the zero article than with the specificity ambiguities of the indefinite article.

The purpose of our study is to clarify about the influence that the visual information gives to the brain activities when pressure exerted the abdomen by waist belts. The visual information means that the some different visual information is inputted it, and the brain activity is evaluated by Electroencephalogram(EEG) measurements. At the same time, we carried out the sensory tests and verified about the relations between the psychological stress and the brain activities. There was a difference in change in the power of the $alpha$ wave with the eyes opened between man and woman. from the result of the sensory test, in the case of under the condition in the darkness with the eyes opened, there was no change in the a wave with before the pressure and after the pressure. In the other cases, $alpha$ wave changed in the same way with before the pressure and after the pressure. In the case of the visible and the invisible in pressure place, $alpha$ wave changed in the same way with before the pressure and after the pressure, but there was a difference in value. From the above, it isn't recognized that the visual information is influencing a pressure sense but some influences are given to it to the brain activities.

Pyruvate decarboxylase (PDC) catalyzes the conversion of pyruvate to acetaldehyde as the penultimate step in alcohol fermentation. The enzyme requires two cofactors, thiamin diphosphate (ThDP) and $Mg^{2+}$, for activity. Zymomonas mobilis PDC shows a strong preference for pyruvate although it will use the higher homologues 2-ketobutyrate and 2-ketovalerate to some extent. We have investigated the effect of mutagenesis of valine 111 and leucine 112 on the substrate specificity. V111 was replaced by glycine, alanine, leucine, and isoleucine while L112 was replaced by alanine, valine, and isoleucine. With the exception of L112I, all mutants retain activity towards pyruvate with $k_{cat}$ values ranging from 40% to 139% of wild-type. All mutants show changes from wild-type in the affinity for ThDP, and several (V111A, L112A, and L112V) show decreases in the affinity for $Mg^{2+}$. Two of the mutants, V111G and V111A, show an increase in the $K_m$ for pyruvate. The activity of each mutant towards 2-ketobutyrate and 2-ketovalerate was investigated and some changes from wild-type were found. For the V111 mutants, the most notable of these is a 3.7-fold increase in the ability to use 2-ketovalerate. However, the largest effect is observed for the L112V mutation which increases the ability to use both 2-ketobutyrate (4.3-fold) and 2-ketovalerate (5.7-fold). The results suggest that L112 and, to a lesser extent, V111 are close to the active site and may interact with the alkyl side-chain of the substrate.

최근 작업기억 훈련을 통한 아동의 학습역량 강화에 대한 관심이 주목받고 있으나, 그 효과성에 대한 의문이 제기되고 있다. 이에, 이 연구는 영역 특수성 기반 작업기억 훈련이 실제 교육 현장에서 취약계층 아동의 작업기억 향상에 기여할 수 있는지 확인하고자 하였다. 이를 위해, D 지역 내 사회서비스 시설 이용 아동을 대상으로 실험집단과 통제집단으로 구분하고, 정보양식 및 기능 특수성 기반 작업기억 훈련 프로그램과 단기기억 훈련 프로그램을 두 집단에 각각 적용한 후, 사전․사후 검사를 통해 집단 간 훈련효과를 비교하였다. 연구 결과, 작업기억의 핵심기능인 조작 및 최신화 능력에서 실험집단이 통제집단보다 더 큰 향상을 보였고, 선택적 주의 및 전환기능에서 두 집단 간 차이가 나타나지 않았다. 이는 영역 특수성 기반 작업기억 훈련 프로그램의 현장 적용의 실제적 효과가 있음을 나타낸다. 이 연구 결과는 영역 특수성 기반 작업기억 훈련이 실제 교육 현장에서 효과를 나타낼 수 있음을 나타내며, 이를 통해 사회서비스 차원에서 취약계층 아동의 학습역량 강화를 위한 작업기억 능력 향상에 기여할 수 있음을 시사한다.

Working memory is a key mechanism of the learning capacity. This study examined whether a domain-specificity-based working memory training program could help improve children' working memory in field situations instead of the laboratory. For this purpose, children using social service centers were recruited and divided into experimental and control groups. The groups were administered using the domain-specificity-based working-memory training program and short-term memory training program, respectively, and the training effects between the groups were compared using pre- and post-tests. The results showed that the manipulation and updating scores were improved strongly in the experimental group compared to the control group, whereas the groups showed similar selective attention and switching. This suggests that the domain-specificity-based working-memory training program has meaningful effectiveness in field applications. Therefore, the current study suggests that providing working memory training programs as a form of social service can enhance the working memory abilities of vulnerable children and improve their learning capacity.

최근 텍스트 분석을 딥러닝에 적용한 연구가 꾸준히 이어지고 있으며, 특히 대용량의 데이터 셋을 학습한 사전학습 언어모델을 통해 단어의 의미를 파악하여 요약, 감정 분류 등의 태스크를 수행하려는 연구가 활발히 이루어지고 있다. 하지만 기존 사전학습 언어모델이 특정 도메인을 잘 이해하지 못한다는 한계를 나타냄에 따라, 최근 특정 도메인에 특화된 언어모델을 만들고자 하는 방향으로 연구의 흐름이 옮겨가고 있는 추세이다. 도메인 특화 추가 사전학습 언어모델은 특정 도메인의 지식을 모델이 더 잘 이해할 수 있게 하여, 해당 분야의 다양한 태스크에서 성능 향상을 가져왔다. 하지만 도메인 특화 추가 사전학습은 해당 도메인의 말뭉치 데이터를 확보하기 위해 많은 비용이 소요될 뿐 아니라, 고성능 컴퓨팅 자원과 개발 인력 등의 측면에서도 많은 비용과 시간이 투입되어야 한다는 부담이 있다. 아울러 일부 도메인에서 추가 사전학습 후의 성능 개선이 미미하다는 사례가 보고됨에 따라, 성능 개선 여부가 확실하지 않은 상태에서 도메인 특화 추가 사전학습 모델의 개발에 막대한 비용을 투입해야 하는지 여부에 대해 판단이 어려운 상황이다. 이러한 상황에도 불구하고 최근 각 도메인의 성능 개선 자체에 초점을 둔 추가 사전학습 연구는 다양한 분야에서 수행되고 있지만, 추가 사전학습을 통한 성능 개선에 영향을 미치는 도메인의 특성을 규명하기 위한 연구는 거의 이루어지지 않고 있다. 본 논문에서는 이러한 한계를 극복하기 위해, 실제로 추가 사전학습을 수행하기 전에 추가 사전학습을 통한 해당 도메인의 성능 개선 정도를 선제적으로 확인할 수 있는 방안을 제시한다. 구체적으로 3개의 도메인을 분석 대상 도메인으로 선정한 후, 각 도메인에서의 추가 사전학습을 통한 분류 정확도 상승 폭을 측정한다. 또한 각 도메인에서 사용된 주요 단어들의 정규화된 빈도를 기반으로 해당 도메인의 특수성을 측정하는 지표를 새롭게 개발하여 제시한다. 사전학습 언어모델과 3개 도메인의 도메인 특화 사전학습 언어모델을 사용한 분류 태스크 실험을 통해, 도메인 특수성 지표가 높을수록 추가 사전학습을 통한 성능 개선 폭이 높음을 확인하였다.

Recently, research on applying text analysis to deep learning has steadily continued. In particular, researches have been actively conducted to understand the meaning of words and perform tasks such as summarization and sentiment classification through a pre-trained language model that learns large datasets. However, existing pre-trained language models show limitations in that they do not understand specific domains well. Therefore, in recent years, the flow of research has shifted toward creating a language model specialized for a particular domain. Domain-specific pre-trained language models allow the model to understand the knowledge of a particular domain better and reveal performance improvements on various tasks in the field. However, domain-specific further pre-training is expensive to acquire corpus data of the target domain. Furthermore, many cases have reported that performance improvement after further pre-training is insignificant in some domains. As such, it is difficult to decide to develop a domain-specific pre-trained language model, while it is not clear whether the performance will be improved dramatically. In this paper, we present a way to proactively check the expected performance improvement by further pre-training in a domain before actually performing further pre-training. Specifically, after selecting three domains, we measured the increase in classification accuracy through further pre-training in each domain. We also developed and presented new indicators to estimate the specificity of the domain based on the normalized frequency of the keywords used in each domain. Finally, we conducted classification using a pre-trained language model and a domain-specific pre-trained language model of three domains. As a result, we confirmed that the higher the domain specificity index, the higher the performance improvement through further pre-training.

The importance of explicit form-focused instruction is well recognized these days and the research has been focused on how to teach grammatical forms. Among several issues concerning instructional styles, this study deals with instructional modes: input-based and/or output-based instruction. There have been conflicting research findings regarding the efficacy of input-based or output-based instruction. To these conflicts the third view is added, which argues that input practice is better for comprehension skills ,and output practice for production skills, seeing language as one of many skills we can learn. Given this background, the present study proposed an experiment to test the argument of skill acquisition theory, and the results indicates that the specificity of practice effect in skill acquisition theory did not work for the learning of the third person singular -s of English.

본 연구에서는 식물의 제초제 해독 기구를 알아보기 위해, 양배추 (Brassica oleracea)로 부터 글루타티온 전달효소를 정제하고 외래성 이물질에 대한 기질 특이성과 저해 효과를 분석하였다. 양배추로부터 DEAE-Sephacel 컬럼과 GSH-Sepharose 컬럼 크로마토그래피를 이용하여 약 10%의 수율로 글루타티온 전달효소를 정제하였다. 정제된 효소에 대해 분자량을 측정한 결과, SDS-polyacrylamide 겔 전기영동으로 측정한 분자량은 23,000Da을 나타내었으며, 겔 크로마토그래피로 측정한 분자량은 48,000Da을 나타내었다. 따라서 정제된 양배추 글루타티온 전달효소는 소단위체의 분자량이 약 23,000Da의 동종이량체라는 사실을 알 수 있었다. 이 효소의 저해제에 대한 효과를 조사한 결과, S-hexyl-GSH와 S-(2,4-dinitrophenyl)GSH에 의해 활성이 저해되었다. 양배추 글루타티온 전달효소의 기질특이성은 CDNB와 ETA에서 높은 활성을 보였으며, cumene hydroperoxide에 대한 GSH peroxidase 활성도 나타내었다.

To gain further insight into herbicide detoxification of plant, we purified a glutathione S-transferase from Brassica oleracea (BoGST) and studied its substrate specificity towards several xenobiotic compounds. The BoGST was purified to electrophoretic homogeneity with approximately 10% activity yield by DEAE-Sephacel and GSHSepharose column chromatography. The molecular weight of the BoGST was determined to be approximately 23,000 by SDS-polyacrylamide gel electrophoresis and 48,000 by gel chromatography, indicating a homodimeric structure. The activity of the BoGST was significantly inhibited by S-hexyl-GSH and S-(2,4-dinitrophenyl)GSH. The substrate specificity of the BoGST displayed high activities towards CDNB, a general GST substrate and ethacrynic acid. It also exhibited GSH peroxidase activity toward cumene hydroperoxide.

본 연구의 주된 목적은 ‘통합적 정보공유 성과모델’을 제안하기 위함이다. 정보공유(information sharing)를 통하여 SCM(Supply Chain Management)내 성과를 향상시켜 줌을 증명하고, 그 현상을 설명해 주는 요소로 자산전용성(asset specificity)을 선정하여 그간의 이론적 관계를 가설로 설정하였다. 즉, 자산전용성이 독립변수로서, 정보공유수준과 정보 공유중요도는 매개변수로서, 종속변수 SCM성과에 유의한 영향을 주는 것을 문헌연구를 통해 가설로 설정하고, 이를 증명하는 ‘정보공유를 통한 성과형성모델’을 제안하고자 하는 것이다. 이는 기존 정보공유를 통한 공급사슬의 효율적 관리에 관한 연구를 제고시키는 학문적 공헌과 함께, 국내외 기업들에게 공급사슬관리를 위한 설명력 있는 모델을 제시하는 실무적 기여가 크다고 할 수 있다. Preliminary Study에서는 정보공유수준과 SCM성과 간의 관계를 확인하였고, Study1에서는 이를 확장하여 정보공유수준과 함께 정보공유중요도가 SCM성과를 설명해주는 유의한 요소인지를 검정하였다. 마지막으로 Study3에서는 (1)자산전용성은 (2)정보공유수준과 (3)정보공유중요도에 영향을 주고, (2)정보공유수준과 (3)정보공유중요도는 완전매개변수로 SCM성과에 영향을 주는 관계를 확인하였고, 이를 ‘정보공유를 통한 성과형성모델’로 확정하였다.

The Purpose of this study is to suggest ‘the Performance Formation Model through Information Sharing.’ In the model, asset specificity is the independent variable, the level of information sharing and the importance of information sharing play roles of the mediators, and SCM(Supply Chain Management) performance is the dependent variable. In Preliminary Study, the positive relationship between the level of information sharing and SCM performance was identified using the data from Delphi Korea Corp. In Study1, which extended the Preliminary study using the data collected from HYUNDAI Mobis, not only the level of information sharing, but also the importance of information sharing was verified as a significant factor affecting SCM level. In Study2, we examined ‘the Performance Formation Model through Information Sharing’using the data from Kyeryong Construction Corp. Finally, we statistically proved (1) asset specificity affects (2) the level of information sharing and (3) the important of information sharing, and then (2) & (3) affect the SCM performance.