Earticle

초록

영어

During the last decade enzyme reaction in organic solvent has been studied to show that specificity in buffer is different from that in organic solvent. The specificity of restriction enzyme was effected by various factors such as ionic strength, salt organic solvent and temperature. In this study, restriction enzyme PvuII which is used most frequently in genetic engineering and the substrate was vector pGEM3 whose sequence was already known were used. As a result the recognition sequence site was changed in the presence of organic solvents whose Log P are -1.5∼0. Their specificities were contrast with activities were contrasted. Specificities were not changed in organic solvent easily in inactivating enzyme. We think that the enzyme recognition site was not changed randomly but by preferential order. A recombinant vector which does not contain typical cleavage site CAG↓CTG was cleaved in 20% ethanol solution. This result might show that restriction enzyme could be used to cleave at unusual sites by changing the reaction conditions.

목차

ABSTRACT
 서론
 실험재료 및 방법
  효소 및 시약
  pGEM3의 분리 정제
  PvuII의 효소반응
  전기영동
  PvuII에 미치는 유기용매의 효과
  PvuII에 미치는 pH, 반응온도, 반응시간의 효과
  PvuII의 절단자리 결정
  PvuII 인식자리가 제거된 재조합 벡터 제조
 실험 결과 
  PvuII에 미치는 유기용매의 효과
  유기용매에서 특이성에 미치는 pH, 온도, 반응시간의 효과
  유기용매에서 PvuII의 절단자리 결정
 고찰
 결론
 감사
 참고문헌

저자

• 김희정 [ Heejung Kim | 전북대학교 자연과학대학 분자생물학과 효소공학연구실 ]
• 이강민 [ Kangmin Lee | 전북대학교 자연과학대학 분자생물학과 효소공학연구실 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576