Earticle

본 논문의 목적은 이스라엘이 이라크와 시리아에 대해 수행하였던 핵 개발시설에 대한 공습의 사례를 통해 예방공격의 효 용성을 살펴보고, 북한에 대해 예방공격을 수행해야 한다면 바람직한 방안을 제시하는데 있다. 군사적 예방공격이 “단기적인 성공은 가능하나 장기적으로는 효과성이 크지 않다”는 주장에 답하기 위해 1981년에 이라크를 대상으로 수행된 Operation Opera와 2007년에 시리아에 감행된 Operation Orchard 수행과정을 살펴보고, 이러한 예방공격 이후에 각 국가의 핵 개발과정 을 살펴본다. 한반도에서 효과적인 예방공격을 위해서는 현재의 국제적 압박 바탕 하에 예방공격의 정당성을 충분히 확보하 고, 1회의 확실한 군사적 공격이 시설 외에도 인력 등에 대해서도 이루어져야 하며, 공격 이후에도 지속적인 핵 개발 재개여부 를 모니터링 해야 한다.

The purpose of this paper is to examine the effectiveness of preventive attacks through cases of military attacks against nuclear facilities in Iraq and Syria that Israel has conducted. It also suggests a desirable approach to preventive attacks against North Korea. The Operation Opera in Iraq in 1981 and the Operation Orchard in Syria in 2007 are examined with an aim to support the opinion “the preventive attack can not be successful in the long run though it may be effective in the short term”. It is also possible to denuclearize if the effective preventive attacks on the Korean peninsula are conducted together with removing both nuclear weapons development means and will. In order to guarantee the successful prevent attacks, it is necessary to secure the legitimacy of preventive attacks as well as international pressure. Also, the reliable military attack should be done on facilities, manpower and monitored continuously to prevent developing the nuclear afterwards.

This study was conducted to investigate whether lysophosphatidic acid (LPA) could improve the development of porcine somatic cell nuclear transfer (SCNT) embryos. Porcine SCNT-derived embryos were cultured in chemically defined polyvinyl alcohol (PVA)-based porcine zygote medium (PZM)-4 without or with LPA, and the development, cell proliferation potential, apoptosis, and expression levels of pluripotent markers were evaluated. LPA significantly increased the rates of cleavage and blastocyst formation compared to those seen in the LPA un-treatment (control) group. The expression levels of embryonic development-related genes (IGF2R, PCNA and CDH1) were higher (p < 0.05) in the LPA treatment group than in the control group. LPA significantly increased the numbers of total, inner cell mass and EdU (5-ethynyl-2’-deoxyuridine)-positive cells in porcine SCNT blastocysts compared to those seen in the control group. TUNEL assay showed that LPA significantly reduced the apoptosis rate in porcine SCNT-derived embryos; this was confirmed by decreases (p < 0.05) in the expression levels of pro-apoptotic genes, BAX and CASP3, and an increase (p < 0.05) in the expression level of the anti-apoptotic gene, BCL2L1. In addition, LPA significantly increased Oct4 expression at the gene and protein levels. Together, our data suggest that LPA improves the quality and development of porcine SCNT-derived embryos by reducing apoptosis and enhancing cell proliferation and pluripotency.

To develop tailored elementary, middle, and high school textbooks suitable for understanding the nuclear energy and radiation, quantitative and qualitative research was carried out in parallel, which included nine steps to ensure the validity of content and structure. The elementary, middle, and high school students wanted to acquire information used in their daily lives, including the definition of nuclear energy and radiation, principles and status of nuclear power generation, and information about irradiated food, medical radiation, and radiation in life. In the evaluation of the effects of textbook contents according to the educational requirements of each school level, high suitability frequencies (>80%) were shown for the human character, education goals, curriculum goals, evaluation method, and education time. At some levels, the high suitability frequencies (>70%) were shown for the education grade, education type, and textbook type.

This study was conducted to investigate nuclear remodeling and developmental rate following nuclear transfer of fetal fibroblast cells, ear skin cells and oviduct epithelial cells into porcine recipient oocytes. To test par-thenogenetic activation, oocytes were treated with a 6-dimethylaminopurine (6-DMAP), a single DC-pulse (DC), calcium ionomycin (ionomycin), DC+6-DMAP and ionomycin + 6-DMAP after in vitro maturation. For nuclear transfer, in vitro matured oocytes were enucleated, and donor cells were transferred into oocytes. Cloned embryos were fused and stimulated with 6-DMAP for 4 h and cultured in vitro for 6 days. Among treatments for parthenogenesis, the activation rate of DC +6-DMAP treatment was significantly higher than that of single treatment roups (p<0.01), except for DC treatment group. However, the difference was not significant in activation rate compared to other complex treatment groups. Nuclear swelling of the cloned embryos was initiated at 60 min after stimulation and increased afterwards. Fusion rates were not different among different donor cells. Cleavage rates of DC treatment groups were significantly higher than those of DC+6-DMAP treatment groups (p<0.05) in case that fetal fibroblast and ear cells were used for nuclear donor. The cloned embryos from developed to blastocysts in oviduct epithelial cell nuclear transfer with DC+6-DMAP treatment was significantly higher compared to those with DC only treatment (p<0.05). However, no blastocyst was developed from nuclear transfer of fetal fibroblast and ear cells regardless of activation treatments. Based on these results, a proper activation stimulation may be necessary to increase the activation rate and the development to blastocyst in cloned porcine embryos.

Interspecies somatic cell nuclear transfer (iSCNT) is a valuable tool for studying the interactions between an oocyte and somatic nucleus. The object of this study was to investigate the developmental competence of in vitro‐matured porcine oocytes after transfer of the somatic cell nuclei of 2 different species (goat and rabbit). Porcine cumulus oocytes were obtained from the follicles of ovaries and matured in TCM‐199. The reconstructed embryos were electrically fused with 2 DC pulses of 1.1 kV/cm for 30 μs in 0.3 M mannitol medium. The activated cloned embryos were cultured in porcine zygote medium‐3 (PZM‐3), mSOF or RDH medium for 7 days. The blastocyst formation rate of the embryos reconstructed from goat or rabbit fetal fibroblasts was significantly lower than that of the embryos reconstructed from porcine fetal fibroblast cells. However, a significantly higher number of embryos reconstructed from goat or rabbit fetal fibroblasts cultured in mSOF or RDH, respectively, developed to the morular stage than those cultured in PZM‐3. These results suggest that goat and bovine fetal fibroblasts were less efficacious than porcine‐porcine cloned embryos and that culture condition could be an important factor in iSCNT. The lower developmental potential of goat‐porcine and porcine‐bovine cloned embryos may be due to incompatibility between the porcine oocyte cytoplasm and goat and bovine somatic nuclei.

Porcine fibroblasts were transferred into enucleated bovine oocytes for the interspecies nuclear transfer (NT). After NT, the embryos were cultured in three different culture systems. The media used for the experiment were CR1aa and NCSU23. The culture systems used for the experiment were: 1. Culture in CR1aa for 7 days (CR). 2. Culture in CR1aa for 2 days and subsequently in NCSU23 for 5 days (CR-NC). 3. Culture in NCSU23 for 7 days (NC). Bovine (intraspecies) NT group was used as a control. The oocytes in bovine NT group were treated the same as interspecies NT embryos except using bovine fibroblasts as nuclear donors. Regardless of their nuclear origin (interspecies vs bovine), the embryos in CR (68.4% vs 77.2%) and CR-NC (67.8% vs 70.5%) showed better developmental competence to the 2-cell stage (p<0.05) than those in NC (41.0% vs 10.0%). Bovine NT embryos in CR-NC did not develop over the 4-cell stage after the medium replacement, while interspecies NT embryos in CR-NC continued to develop and could reach over the 8-cell stage (12.2%). Blastocysts were only found in bovine NT group (17.4%), but no blastocyst was found in interspecies NT group. This study suggests that the development of interspecies NT embryos mostly depends on their recipient cytoplasm during the culture in vitro.