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In Vitro Development of Interspecies Nuclear Transfer Embryos using Porcine Oocytes with Goat and Rabbit Somatic Cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 32 No 4 (2008.12)바로가기
  • 페이지
    pp.249-253
  • 저자
    Yan Shi Quan, Kenji Naruse, Su Min Choi, Myung Youn Kim, Rong Xun Han, Chang Sik Park, Dong Il Jin
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A98933

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원문정보

초록

영어
Interspecies somatic cell nuclear transfer (iSCNT) is a valuable tool for studying the interactions between an oocyte and somatic nucleus. The object of this study was to investigate the developmental competence of in vitro‐matured porcine oocytes after transfer of the somatic cell nuclei of 2 different species (goat and rabbit). Porcine cumulus oocytes were obtained from the follicles of ovaries and matured in TCM‐199. The reconstructed embryos were electrically fused with 2 DC pulses of 1.1 kV/cm for 30 μs in 0.3 M mannitol medium. The activated cloned embryos were cultured in porcine zygote medium‐3 (PZM‐3), mSOF or RDH medium for 7 days. The blastocyst formation rate of the embryos reconstructed from goat or rabbit fetal fibroblasts was significantly lower than that of the embryos reconstructed from porcine fetal fibroblast cells. However, a significantly higher number of embryos reconstructed from goat or rabbit fetal fibroblasts cultured in mSOF or RDH, respectively, developed to the morular stage than those cultured in PZM‐3. These results suggest that goat and bovine fetal fibroblasts were less efficacious than porcine‐porcine cloned embryos and that culture condition could be an important factor in iSCNT. The lower developmental potential of goat‐porcine and porcine‐bovine cloned embryos may be due to incompatibility between the porcine oocyte cytoplasm and goat and bovine somatic nuclei.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

저자

  • Yan Shi Quan [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
  • Kenji Naruse [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
  • Su Min Choi [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
  • Myung Youn Kim [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
  • Rong Xun Han [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
  • Chang Sik Park [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
  • Dong Il Jin [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 32 No 4

    피인용수 : 0(자료제공 : 네이버학술정보)

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