Earticle

The term of nutri-cosmetics refers to nutritional supplements which can support the function and the structure of the skin. The fisetin is a flavonol which belongs to the flavonoid group of polyphenols. Although the anti-carcinogenic property of fisetin have been widely studied, anti- oxidative function of fisetin in HaCaT keratinocytes is unknown. In this study, we examined the in vitro chemical and cellular anti-oxidant activities of fisetin in HaCaT keratinocytes and we further discussed its potential as an anti-oxidant nutricosmetics. We employed scavenging assay for the 1,1-diphenyl-2,5-picrylhydrazyl (DPPH) radicals and cellular anti-oxidant activity of fisetin was investigated in H2O2-treated HaCaT keratinocytes as judged by the 2’7’-dichlorofluorescein-diacetate (DCFH-DA)-based assay. The fisetin effectively scavenged DPPH radicals (IC50 54 μM) when compared to the scavenging activities of L-ascorbic acid (IC50 87 μM) acid and trolox (IC50 74 μM), well-known anti-oxidants. In addition, the fisetin significantly ameliorates H2O2-induced oxidative stress in HaCaT keratinocytes in dose-dependent manner. The fisetin showed more strong cellular anti-oxidant activity than those of L-ascorbic acid and trolox in H2O2-treated HaCaT keratinocytes. In agreement with result of the cellular anti-oxidant activity, we observed markedly suppressed H2O2-induced oxidative stress by the pretreatment of 100 μM fisetin under the fluorescence microscopy analysis. These results provide the scientific evidences for the development of skin targeted nutri-cosmetics which has anti-oxidant function.