Earticle

To conduct a risk assessment of AFB1 intake, AFM1, which is a metabolite of AFB1 in the human and porcineurine, was determined by competitive direct ELISA (cdELISA). The detection limit of cdELISA using anti-AFM1 antibodyand AFB1-HRP conjugate was 10pg/mL. The recoveries of AFM1 were 117-167% after the addition of AFM1 in the humanurine in a range of 3-100pg/mL. 165 samples (95.5%) of those obtained from 172 persons evidenced measurable levels ofurinary AFM1. The detected AFM1 ranges were 0-11.6pg/mL and the average level of AFM1 contamination was 2.74±1.89pg/mL. The estimated amount of AFM1 excretion in the human urine was 3.97ng/day and the estimated AFB1 intakeamount was 79.4ng/day. The probable daily intake (PDI) of AFB1 by the subjects was estimated to be 1.28ng/kg.bw/day,which was higher than the tolerable daily intake (TDI, 0.15ng/kg.bw/day). In the case of porcine urine, the AFM1 rangedbetween 0.97-26.7pg/mL and the average contaminated AFM1 was 10.62±4.39pg/mL. The estimated amount of AFM1 excretionin the porcine urine was 27.6ng/day, and the estimated AFB1 intake amount was 551ng/day.