Earticle

본 연구에서는 hG-CSF의 발현을 유도적으로 조절하기 위한 FIV-Tet-On lentivirus vector system을 구축하고자 하였다. hG-CSF는 호중성구 계열 세포의 증식과 분화, 생존에 영향을 미치는 물질로서, 이 유전자의 발현을 증가시키기 위하여 FIV-Tet-On vector 상의 hG-CSF나 rtTA2SM2 서열의 3' 위치에 WPRE 서열을 도입하였다. 구축된 각각의 vector는 293FT 세포에 일시적으로 transfection하여 virus를 생산하였으며, 이 virus를 일차 배양 세포인 CEF와 PFF에 감염시켰다. 각 세포에 전이된 hG-CSF의 발현 양상을 관찰하기 위하여 doxycycline을 첨가하거나 첨가하지 않은 배지에서 배양한 후 quantitative real-time PCR, Western blot과 ELISA를 이용하여 hG-CSF 유전자의 발현 정도를 비교 측정한 결과, CEF에서는 WPRE가 hG-CSF의 3' 위치에 도입된 경우에 발현량과 유도율이 가장 높은 것으로 나타났고, PFF에서는 rtTA 서열의 3'위치에 도입된 경우에 발현량과 유도율이 가장 큰 것으로 확인되었다. 이 FIV-Tet-On vector system은 형질 전환 동물의 생산이나 유전자 치료에서 문제시되는 외래 유전자의 지속적인 과다 발현에 의한 개체의 생리적인 부작용을 최소화하기 위한 해결 방법으로 제시될 수 있을 것이다.

In this study, using FIV-based lentivirus vector system, we tried to express hG-CSF in tetracycline-controllable manner. hG-CSF influences the proliferation, differentiation, and survival of cells in the neutrophil lineage. To enhance stability and translation of hG-CSF transcript, WPRE sequence was also introduced into FIV-Tet-On vector at downstream region of either the hG-CSF gene or the sequence encoding rtTA. Primary culture cells (CEF, chicken embryonic fibroblast; PFF, porcine fetal fibroblast) infected with the recombinant FIV were cultured in the medium supplemented with or without doxycycline for 48 hours, and induction efficiency was measured by comparing the hG-CSF gene expression level using quantitative real-time PCR, Western blot and ELISA. Higher hG-CSF expression and tighter expression control were observed from the vector in which the WPRE sequence was placed at downstream of the hG-CSF (in CEF) or rtTA (in PFF) gene. This FIV-Tet-On vector system may be helpful in solving serious physiological disturbance problems which has continuously hampered successful production of transgenic animals and gene therapy.

Speed-regulation characteristic of PMSM (permanent magnet synchronous motor) in marine electric propulsion system is studied in this paper. Governing method based on vector control is analyzed according to the mathematical model in two-phase rotating coordinate system. After that, a noble PID control strategy which raises dual closed-loop i.e. speed loop and stator current loop is proposed based on fuzzy control theory. Speed deviation and its rate are taken as inputs for adjustment of the PID controller. The strategy proposed in this paper improves the response of system, static and dynamic characteristics of PMSM servo system as well as anti-jamming capability. Finally, simulation results from Simulink show the correctness and realizability of our system.

다중 모터를 효과적으로 제어하기 위하여 신호 측정 및 제어 알고리즘 수행 관련 스케쥴링 방법을 제안하고 제어 성능을 검토한다. 차량 내 전자제어시스템과 연계되어 동작하는 다중 모터 제어기는 CAN(Controller Area Network) 통신을 통하여 위치/속도 지령 값을 전달 받고 각 모터에 대한 위치/속도 제어, 전류 제어 등을 수행한다. 하나의 마이크로컨트롤러를 이용하여 다수 대 모터를 효과적으로 제어하기 위하여 내부 자원과 알고리즘 수행에 대한 적절한 스케쥴링이 중요하다. ADC(Analog to Digital Converter) 타이밍과 제어 알고리즘 수행 타이밍을 다양하게 변화시키며 실제 실험을 통하여 다중 모터 제어기의 성능을 분석한다.

In this paper, we propose a scheduling method for signal measurement and control algorithm execution in a multi-motor drive controller. The multi-motor controller which is used for vehicle control receives position/velocity command and performs position/velocity control and current control. Internal resource allocation and control algorithm execution timing are very important when one microcontroller is used for multi-motor drives. The control performance of the velocity control system is verified by varying ADC(Analog to Digital Converter) conversion timing and algorithm execution timing using real experiments.

본 논문에서는 다중 모터 기반 피드백 제어 시스템에 대하여 캐스케이드 형태의 제어기를 고려하고 제한된 ADC(Analog to Digital Converter) 자원을 효과적으로 이용하기 위한 자원 할당 방법을 제안한다. 이 방법의 목적은 한정된 ADC 자원으로 모터 위치 측정값의 오차 및 d-q 축 전류 측정값의 오차를 최소화하는 것이다. 다중 모터 기반 피드백 제어 시스템에 대한 캐스케이드 제어 구조는 속도 제어기와 전류제어기로 구성되며, 이러한 구조가 각 모터에 병렬로 적용되어 각 모터의 회전 속도가 독립적으로 제어된다. 다중모터제어기는 추가되는 마이크로컨트롤러 없이 오직 하나의 컨트롤러에 구현된다. 다수의 AC 모터로 구성된 실험환경과 제안된 제어구조 및 ADC 할당방법을 이용함으로 써 각 모터의 속도 및 토크가 주어진 속도 지령값을 정밀하게 추종함을 보인다.

In this paper, we consider a cascaded type of control architecture for a multi motor-based feedback control system and propose an ADC (Analog to Digital Converter) resource allocation method to efficiently utilize the limited ADC resources. The purpose of the resource allocation method is to minimize both the motor position measurement error and the d-q current measurement error. The cascaded type of control architecture is applied in parallel to each motor to independently control the speed of a motor in the multi motor control system. All the control algorithms are implemented by software using a single microcontroller without using additional microcontrollers. It is illustrated by experiments that the speed and the torque of each motor are controlled precisely by the proposed control architecture with the efficient ADC allocation method.

The Attitude control model for AUV is highly nonlinear and strong coupling six degrees of freedom model. With it, and complex disturbance of the marine environment a new control strategy chaos least squares support vector machines is proposed. The control model is infinitely approximated by least squares support vector machine. The parameters are optimized Chaos theory. It is formed the Chaos-LSSVM model. The purpose is to obtain excellent attitude control of quality. The Chaos-LSSVM predictive controller for AUV attitude is designed combined with a simplified longitudinal hydrodynamic equations and the Chaos-LSSVM model. Meanwhile the stability of the attitude control system is ensured by the Lyapunov stability theory. Finally, examples of simulation results show that the control model can obtain a good control effect, improve the underwater vehicle dynamic positioning attitude control effectiveness and robustness.

Decoupling control of two-motor dual three-phase permanent-magnet synchronous motor (PMSM) series-connected system can be realized by using proper phase transposition rules with a single supply from a six-phase voltage source inverter (VSI), which solved the control problem of two motors in series. Since the traditional two-vector SVPWM strategy, which is commonly used in three-phase system, exists 5th and 7th harmonics in x-y plane, making it hard to realize decoupling control of two-motor dual three-phase PMSM series-connected system. Meanwhile, the DC bus voltage utilization of carrier-based PWM strategy is lower. Thus one method, which adopted a TBTSB-SVPWM strategy with two modulators, was proposed in this paper. It ensures the inverter output voltage only contains two kinds of fundamental components required by the two series-connected motors with a single VSI, making it possible to realize decoupling control of two-motor dual three-phase PMSM series-connected system. Simulation results indicate that there is no influence on the other motor while the speed/load of any one motor changed, achieving the decoupling control of series-connected system, and the feasibility and validness of the proposed approach are verified.

This paper proposes a control mode switching scheme between vector control and constant V/f control for induction machine (IM) drives for maximum torque utilization in a higher speed region. For the constant V/f scheme, a smooth transition method from the linear range of PWM up to the six-step mode is applied, by which the machine flux and torque can be kept constant in a high-speed range. Also, a careful consideration of the initial phase angle of the voltage in the transient state of the control mode change between the vector control and V/f schemes is described. The validity of the proposed strategy is verified by the experiment result for a 3-kW induction motor drives.

This paper presents the control algorithm for maximum efficiency drives of an induction motor system with the high dynamic performance. This system uses a simple model of the induction motor that includes equations of the iron losses. The model, which only requires the parameters of the induction motor, is referred to a field-oriented frame. The minimum point of the input power can be obtained at the steady state condition. The proposed optimal efficiency control algorithm calculates the reference torque and flux currents for the vector control of the induction motors. A 32 bit floating point TMS320C32 DSP chip implements the drive system with the efficiency optimization controller. The results show the effectiveness of the control strategy Proposed for the induction motor drive.

In this paper, a current hysteresis control with good decoupling properties for doubly-fed brushless induction machines (BDFIMs) has been proposed based on a generalized vector model. The independent control of the reactive power and speed for BDFIMs has been achieved by controlling the d-axis and the q-axis current of the control windings (CW). The proposed vector control method has been developed for the power winding (PW) flux frame. Experimental verification of a type Y180M-4 BDFIM prototype with 1/4 pole-pairs has been presented. Evidence of its good performance has been shown through experimental results.

In the High-Speed range for salient type synchronous reluctance motors, the effect of iron loss can not be negligible. In this paper, the SynRM without cage is analyzed mathematically to model and the speed control system is examined by simulation. In order to control the revolution speed, a closed-loop control with vector control and decoupling control are applied to the high-speed SynRM drives. The speed control system is analyzed to investigate the desired speed characteristics of high SynRM by simulation.

polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) 유전자로 구성된 재조합 베큘로바이러스를 제작하였다. 본 재조합 베큘로바이러스 시스템은 여러가지 세포주와 여러 가지 조직에 감염하여 시험하였고 재조합된 유전자의 전달과 유전자 발현을 대조 벡터시스템과 비교하였다. 본 연구의 결과로 제작된 재조합 베큘로바이러스 시스템은 유전자의 전달과 발현에 있어서 대조 벡터시스템 보다 효능과 안전성면에서 우수하였다.

A recombinant baculovirus vector systems were composed of genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD). These recombinant baculovirus vector system were transfected into various cell lines and tissues and confirmed gene transfer and expression of these vector systems with only control vector system. From the result, gene transfer and gene expression of recombinant baculovirus vector systems were superior in terms of efficacy and safety than in the control vector system.

A method of speed sensorless vector control of induction motor is proposed in this paper. This method uses the slip frequency estimated by only the primary voltage and current. As this slip frequency accords with the command value of it, the commanded primary frequency is controlled. The validity of the method is confirmed by the simulation and experimental results.

This paper presents a impletation of vector control of induction motor using Matlab/Simulink and dSPACE DS1104. System consists of digital input block, digital ouput block, ADC block, protection block, motor control block and PWM block. It is applied indirect vector control and PI controller to speed controller and current controller.

This paper presents the realization of the vector control for AC servo motor using a high performance DSP, TMX320F2812. This DSP has some special peripheral circuits to drive a AC Servo motor as AD converter, QEP and so on. It makes us reduce the time of developing a control system and also can be simple size controller. The vector control algorithm for instantaneous torque control and SVPWM algorithm by offset voltage methods is adopted and we got the satisfactory results.

Speed sensorless modes of operation are becoming standard solution in the area of electric drives. This paper presents flux estimator and speed estimator for the speed sensorless vector control of induction motors. The proposed sensorless methods are based on the model reference adaptive system (MRAS) observer and adaptive speed observer (ASO). The proposed speed estimation algorithm can be employed in the power control of grid connected induction generator for wind power applications. Two proposed schemes are verified through computer simulation PSIM and compared their simulation results.

This paper presents a voltage control algorithm for a hybrid multilevel inverter based on a staged-perception of the inverter voltage vector diagram. The algorithm is applied to control a three-stage eighteen-level hybrid inverter, which has been designed with a maximum number of symmetrical levels. The inverter has a two-level main stage built using a conventional six-switch inverter and medium- and low- voltage three-level stages constructed using cascaded H-bridge cells. The distinctive feature of the proposed algorithm is its ability to avoid the undesirable high switching frequency for high- and medium- voltage stages despite the fact that the inverter's dc sources voltages are selected to maximize the number of levels by state redundancy elimination. The high- and medium- voltage stages switching algorithms have been developed to assure fundamental switching frequency operation of the high voltage stage and not more than few times this frequency for the medium voltage stage. The low voltage stage is controlled using a SVPWM to achieve the reference voltage vector exactly and to set the order of the dominant harmonics. The inverter has been constructed and the control algorithm has been implemented. Test results show that the proposed algorithm achieves the desired features and all of the major hypotheses have been verified.

본 논문에서는 MATLAB/SIMULINK와 dSPACE DS1104보드를 이용하여 유도전동기의 간접백터제어 알고리즘을 구현하였다. 유도전동기의 간접벡터제어를 위한 전체 시스템 모델은 SIMULINK 그래픽 모델과 dSPACE DS1104 R&D Control 보드의 Real Time Interface(RTI)를 이용하여 구현한 후에 실험을 수행하였다. 벡터제어를 위해서 전동기 전류의 측정은 16kHz로 측정하였고, 측정된 전류를 이용하여 8Hz로 전류제어기, 4kHz로 속도제어기를 구현하였다. MATLAB과 dSPACE 보드의 컴파일러가 설계된 그래픽 모델의 C source 파일과 object 파일을 자동으로 생성시켜주기 때문에 추가적인 프로그램의 coding 없이 실제 시스템의 시뮬레이션과 실험을 동시에 수행할 수 있으며 원하는 제어성능을 얻을 수 있다.