Earticle

인체의 두피 각질에서 유산균 Leuconostoc mesenteroides 을 분리하여 배양하고 이를 함유한 가용화 에센 스를 피부에 첩포한 후 자극 유무 검증을 통해 화장품의 소재로 활용 가능성을 판단하고자 하였다. 1% 농도로 희석 한 L. mesenteroides 배양액과 이를 1% 함유한 에센스를 제조하여 L. mesenteroides 배양액은 31명, 에센스는 32명의 피시험자들의 등 부위에 24시간 첩포하고 이를 제거한 후 30분, 24시간, 48시간 후의 상태를 피부과 전문 의가 육안으로 판독하여 자극 지수를 산출하였다. 이 결과 L. mesenteroides 배양액은 0.011점, 에센스는 0점이 도출되어 모두 무자극으로 피부에 안전한 것으로 나타났다. 본 연구에서의 일차자극시험 결과와 선행 연구에서 보 고된 항알레르기, 항산화, 항염 활성능 등을 미루어 보아 저 농도의 L. mesenteroides 배양액은 피부에 안전하여 향후 기초화장품에 적용 시 피부자극이 없는 소재로 활발한 연구가 가능할 것으로 기대된다.

It was to establish the usage possibility as cosmetic material by testing stimulation of Leuconostoc mesenteroides which were cultured from human scalp’s dead skin cell by patching it to the skin in the form of solubilized essence. Cultured L. mesenteroides diluted to 1% concentration and essence which contains it were patched to 31 and 32 participants’ back each for 24 hours and established the stimulus figures by the specialists in 30 minutes, 24 hours, and 48 hours after they were taken off. They are both revealed as non-stimuli due to the result of 0.011 and 0 points each and therefore they are safe when used as skin products. Considering the results of this test and the other previous studies which reported the L. mesenteroides as anti-allergic, antioxidant, and anti-inflammatory, L. mesenteroides is usable for cosmetics and worth further research.

목적: 본 연구는 Derma GenieTM 및 Elixir StoneTM 유효 성분을 함유한 패치 제품의 종아리 붓기 감소, 혈행 개선, 피부 온도(온열) 상승에 대한 인체효능평가를 진행하고자 한다. 방법: 본 연구는 만 20-60세의 성인여성 20명을 대상으로 4주동안 패치 제품의 종 아리 붓기 감소, 혈행 개선, 피부 온도(온열) 상승효과에 대한 인체적용시험을 실시하였다. 결과: 종아리 붓기 감소 평가 결과, 패치 미부착 부위와 비교 시, 종아리 붓기가 통계적으로 유의하게 감소되었다(p<0.05). 혈행 개선 평가 결과, 부착 부위의 변화율이 통계 적으로 유의하게 높아 혈행 개선에 도움됨을 확인하였다(p<0.05). 피부 온도 상승 평가 결과, 패치 사용 전과 비교하여 패치 부착부 위에서 피부 온도 값이 1회 사용 후 6.84% 통계적으로 유의하게 증가되어 단 1회 사용만으로 피부 온도 상승(온열) 효과가 있음을 확인하였다(p<0.05). 결론: Derma GenieTM 및 Elixir StoneTM 유효 성분으로 제조한 패치 제품의 종아리 붓기 감소, 혈행 개선, 피부 온도(온열) 상승에 효과적임을 확인하였다.

Purpose: This study evaluated the human efficacy of a patch product formulated with the active ingredients Derma Genie™ and Elixir Stone™ in reducing calf swelling, improving blood circulation, and increasing skin temperature (thermogenic effect). Methods: A human application test was conducted for 4 weeks on 20 adult female participants aged 20–60 years to assess the effects of the patch product on the three variables mentioned above. Results: The evaluation of calf swelling demonstrated a statistically significant decrease in swelling compared with that in the untreated area (p<0.05). In the assessment of blood circulation, the rate of change in the patch-applied area was significantly higher, confirming its beneficial effect of enhancing circulation (p<0.05). Finally, the skin temperature evaluation showed a statistically significant increase of 6.84% in the patch-applied area after a single application compared with the baseline, supporting its immediate thermogenic effect (p<0.05). Conclusion: These findings confirm that the patch product formulated with the active ingredients Derma Genie™ and Elixir Stone™ is effective at reducing calf swelling, improving blood circulation, and increasing skin temperature.

目的: 本研究旨在评估含有Derma GenieTM和Elixir StoneTM有效成分的贴片产品在缓解小腿浮肿、促进血液循 环以及提高皮肤温度(热感)方面的功效。方法: 本研究以20名20-60岁的成年女性为研究对象，进行为期4周的临 床评估试验，评价贴片产品对缓解小腿浮肿、改善血液循环及提升皮肤温度(热感)的效果。结果: 缓解小腿浮肿 的评价结果为，与未使用贴片的对照部位相比，使用贴片部位小腿浮肿显著减少，统计学分析显示差异具有显 著性(p <0.05)；改善血液循环的评价结果为，使用贴片部位的血液流动变化率显著提高，证实该产品有助于促 进血液循环(p <0.05)；提升皮肤温度(热感)的评价结果为，与使用前相比，使用贴片部位的皮肤温度在单次使用 后立即显著上升6.84%，表明该产品仅一次使用即可产生明显的热感效果(p <0.05)。结论: 研究结果表明，含有 Derma GenieTM及Elixir StoneTM有效成分的贴片产品对缓解小腿浮肿、改善血液循环及提高皮肤温度(热感)具有 显著效果。

다양한 의료 응용 분야에서 웨어러블 및 피부 부착형 전자 패치에 피부 표면의 높은 접착력과 내수성이 요구 된다. 본 연구에서는 탄소 기반 전도성 고분자 복합 소재에 개구리 발바닥의 육각 채널와 문어 빨판의 흡착 구조 패 턴을 모사한 신축성 있는 전자 패치를 보고한다. 개구리의 발바닥을 모사한 육각 채널 구조는 수분을 배수하며, 균열 억제 효과를 통해 점착력을 향상 시키며, 문어 빨판을 모사한 흡착 구조는 젖은 표면에서 높은 점착력을 나타낸다. 또한 고점착 전자패치는 실리콘(max. 4.06 N/cm2), 피부 복제 표면(max. 1.84 N/cm2) 등 다양한 표면에 건조 및 젖 은 조건에서 우수한 접착력을 가지고 있다. 고분자 매트릭스와 탄소 입자를 기반으로한 고분자 복합소재를 통해 제작 된 고점착 전자 패치는 건조 및 습한 환경에서 심전도(ECG)을 안정적으로 감지할 수 있다. 이 연구에서 보여진 특성 을 기반으로 제안된 전자 패치는 다양한 생체 신호의 진단을 위한 웨어러블 및 피부 부착 센서 디바이스를 구현하는 잠재적 응용 가능성을 제시한다.

High adhesion and water resistance of the skin surface are required for wearable and skin-attachable electronic patches in various medical applications. In this study, we report a stretchable electronic patch that mimics the drainable structure pattern of the hexagonal channels of frog's pads and the sucker of an octopus based on carbon-based conductive polymer composite materials. The hexagonal channel structure that mimics the pads of frogs drains water and improves adhesion through crack arresting effect, and the suction structure that mimics an octopus sucker shows high adhesion on wet surfaces. In addition, the high-adhesive electronic patch has excellent adhesion to various surfaces such as silicone wafer (max. 4.06 N/cm2) and skin replica surface (max. 1.84 N/cm2) in dry and wet conditions. The high skin-adhesive electronic patch made of a polymer composite material based on a polymer matrix and carbon particles can reliably detect electrocardiogram (ECG) in dry and humid environments. The proposed electronic patch presents potential applications for wearable and skin-attachable electronic devices for detecting various biosignals.

166Ho, a $\beta$-emitting radionuclide, was incorporated within polyurethane film for possible application for the therapy of skin cancers. The aim of this study was to investigate skin irritant after radiation with 166Ho patch in rabbits and to estimate the efficacy of this therapy for skin cancer patients. Six NZW rabbits were used for skin irritant in this study. The dorsal hair of rabbits was removed with an electric clipper and blade. (omitted)

Background: Atopic dermatitis (AD) is frequently associated with food allergies. In addition to the skin prick test (SPT) and serum-specific IgE, the atopy patch test (APT) has been introduced as a diagnostic procedure for food allergies. Objective: Our aim was to evaluate the diagnostic value of the APT, the SPT and the serum-specific IgE levels compared with that of oral food challenge test against milk and egg in AD patients. Methods: We conducted the SPT and APT, and determined the serum-specific IgE levels against milk and egg antigens for 101 patients. Oral food challenge tests were conducted for 86 out of 101 AD patients. The sensitivity,specificity and positive and negative predictable values were calculated for all the tests. Results: Twenty-five patients were positive to oral food challenges. The sensitivity of the APT for milk was 66.7%, while the figures for the SPT and the serum-specific IgE were 35.5% and 14.2%. The sensitivity of the APT for egg was 50%, while that for the SPT and serum-specific IgE were 21.4% and 6.7%. Conclusion: We were able to conclude that the APT test seems to be a valuable additional tool for the diagnostic method of food

Background: Hyaluronic acid (HA)-based microneedle patch has recently been studied for wrinkle improvement. Cross-linked HA (CLHA) is widely used in dermal fillers. Acetyl hexapeptide-8 (AHP-8) and epidermal growth factor (EGF) are used for cosmetic ingredients. Objective: This study aimed to verify the efficacy of the CLHA/HA-based patch with microstructure (microneedle patch) containing AHP-8 or EGF. Methods: A total of 52 Korean females were enrolled in a double-blind, randomized, controlled, splitface trial. The subjects were divided into 3 groups: (1) microneedle patch alone, (2) microneedle patch/AHP-8, and (3) microneedle patch/EGF. The treatment was applied on the periorbital and nasolabial fold area for 4 hours to completely dissolve the microstructures once per week for 29 days. Evaluations, including photodamage scoring, image analysis with Antera 3Dⓡ (Miravex, Ireland), skin hydration measurement, and adverse effect assessments, were performed at each visit. Results: Fifty subjects (96.2%) completed this clinical study. On day 29 after application, statistically significant improvements in wrinkle and skin hydration were observed in all groups (p＜0.01). Treatment with microneedle patch/AHP-8 and microneedle patch/EGF showed statistically significant improvements in wrinkle compared with microneedle patch alone (p＜0.05). No serious adverse effects were noted. Conclusion: Combination of CLHA-based microneedle patch and functional cosmetic ingredients can improve wrinkle with minimal discomfort.

Clonidine, an antihypertensive drug, stimulates postsynaptic $\alpha_2$adrenergic receptors in the CNS and lowers arterial pressure by erects on both cardiac output and peripheral resistance. However, many patients experience that sedation and xerostomia occured upon oral administration of clonidine. These side effects are due to high plasma peak concentration and can be avoided when clonidine is given transdermally. In this study, we performed the skin irritation test for transdermal administration of clonidine patch on New Zealand white rabbits. Twelve New Zealand white rabbits were divided into two groups according to the dose levels, respectively. After transdermal administration of clonidine patch with two doses, clinical manifestations, body weight loss and postmortem findings were observed. We could not find any significant evidence of skin irritation by transdermal administration of clonidine patch.

Anti-inflammatory and analgesic activities and skin irritation of piroxicam patch were investigated. Piroxicam patch increased the pain threshold in rat hind paw inflamed by carrageenan and inhibited writhing induced by acetic acid in mice. Piroxicam patch also inhibited the carrageenan-induced edema in rat hind paw as well as the increased vascular permeability induced by histamine in rats. In adjuvant arthritis of rats, piroxicam patch showed anti-inflammatory effects. Skin irritation of piroxicam patch was tested in Newzealand White rabbits and evaluated by Primary Irritation Index of Draize. The results from skin irritation test showed that piroxicam patch seemed practically non-irritating. The result from the present study indicates that piroxicam may be useful without serious side effects as anti-inflammatory analgesics in this patch form.

자궁경부암이 수술 절개부위에 재발하는 경우는 매우 드물다. 본 저자들은 개인 산부인과에서 자궁의 양성질환 진단하 전자궁 적출술 시행 후 자궁경부암으로 진단되어 본원으로 전원, Cisplatin-5FU 동시 항암방사선요법 치료 후 추적관찰 중 5개월 후 절개부위에 재발된 한 예를 경험하였다. 재발부위 종양을 광범위 절제 후 근막 결손 부위를 Gore-Tex® 패치를 이용하여 봉합 후 피부근막 피판으로 피부 복원을 시행하였다. 수술 후 cisplatin 동시 항암방사선요법 시행 후 합병증 및 재발 없이 추적관찰 중인 한 예를 간단한 문헌고찰과 함께 보고하는 바이다.