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한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.93
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.94
OPU에 있어 채란기간에 따른 한우 난자회수율과 분할율
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.95
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.96
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.97
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 pp.98-99
Effect of Glutathione on In Vitro Development in Porcine Tetraploid Embryo
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.100
Effects of Uterine Fluid on In Vitro Development in Porcine Embryos
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.101
The Effect of Various Assisted Hatching Techniques on the Mouse Early
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.102
Co-culture with Good COCs Enhances the Rates of Maturation and Development of Poor COCs
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.103
SETD2 and Tri-Methylation of Histone H3K36 in Porcine Parthenogenetic Embryos
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.104
Cathepsin B Inhibitor E-64 Enhances Developmental Rate and Reduces Apoptosis in Porcine Parthenotes
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.105
Cathepsin B, a lysosomal cysteine protease of the papain family, has recently been implicated in quality and developmental competence of bovine preimplantation embryos. In this study, to determine whether inhibition of Cathepsin B activity could improve oocyte maturation and early embryo developmental competence in porcine, we added E-64, an inhibitor of Cathepsin B, into the in vitro maturation and culture media. Both mRNA and protein expression were no differences in germinal vesicle (GV) stage oocytes between good and poor quality from the morphology. Even activity of Cathepsin B is high in good quality GV stage oocytes, E-64 did not enhance in vitro maturation of porcine oocyte. However, I nM E-64 significantly (p<0.05) increased blastocyst formation and cell numbers. Inhibition of Cathepsin B activity did not alter mitochondrial membrane potential, copy numbers and ATP contents. But, E-64 significantly (p<0.05) reduced mitochondrial release of cytochrome c, finally decreased mRNA expression of Caspase 3 and Caspase 9, resulted in reduction of apoptotic index in porcine blastocysts. Collectively, these observations suggest that Inhibition of Cathepsin B activity enhanced developmental competence and reduced apoptosis through protecting against cytochrome C release from mitochondria in porcine parthenotes.
Iron Depletion Decreases ROS in Porcine Early Embryos Developing In Vitro
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.106
Iron is an important element which takes part in several biological processes. The role of iron in porcine oocyte maturation and early embryo development was still unknown. In the present study, iron was depleted by deferoxamine (DFM), a specific iron chelator, during in vitro maturation (IVM) and in vitro culture (IVC) period respectively. Results showed that, oocytes were matured with 1 μM DFM for 44 h followed by parthenogenetic activation (PA) displayed higher (p<0.05) blastocyst rate compared to without DFM in IVM medium. Another hand, supplemented with 0.5 μM DFM to the IVC medium for 7 days enhanced blastocyst formation both in PA and in vitro fertilization (IVF) groups (p<0.05). Although there was no difference in total cell number in IVF-derived blastocysts between control and iron depleted group, apoptotic index was significantly (p<0.05) decreased following iron depletion. ROS content in MII oocyte and blastocyst was significantly (p<0.05) decreased after iron depletion. For clarify the mechanism of the phenomenon, expressions of apoptotic- and ROS-related genes in the blastocyst were checked. Iron depletion reduced the expression of Caspase 3 and increased Bcl-xL in blastocyst (p<0.05), but there was no significanly differenc in m-RNA expression of Nox4. Mitochondrial membrane potential was increased both in MII oocytes and blastocysts after DFM treatment. In conclusion, iron depletion decreased ROS in oocytes and embryos developing in vitro, further enhanced mitochondrial activity and reduced the apoptosis in blastosyst, resulted in enhancement of the developmental capacity in porcine early embryos.
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.107
Increased PlGF Promotes Gestational Loss
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.108
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.109
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.110
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.111
개의 발정단계에 따른 난소, 난관조직에서 BDNF와 그 수용체 발현 분석
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.115
The Expression Change of Steroid Hormone Receptors during the Estrous Cycle in Oviduct of Canine
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.116
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.119
조직 특이 중간엽 줄기세포(Mesenchymal Stem Cells, MSCs)의 Epigenetic Chromatin Modification
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 pp.120-121
Enhanced Direct Conversion into Hepatocytes by Additional Transcription Factor
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 pp.122-123
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.123
Embryonic stem (ES) cells are pluripotent cells with the capacity for unlimited self-renewal or differentiation. Inhibition of MAPK pathways enhances mouse ES cell pluripotency characteristics. Compared to wildtype ES cells, jnk2(—/—) ES cells displayed a much higher growth rate. To determine whether JNKs are required for stem cell selfrenewal or differentiation, we performed a phosphorylation kinase array assay to compare mouse ES cells under LIF+ or LIF- culture conditions. The data showed that activation of JNKs was induced by LIF withdrawal. We also found that JNK1 or 2 phosphorylated Klf4 at threonines 224 and 225. Activation of JNK signaling and phosphorylation of Klf4 inhibited Klf4 transcription and transactivation activity. Importantly, jnk1 (—/—) and jnk2(—/—) murine embryonic fibroblasts (MEFs) exhibited a significantly greater potency in the ability to increase the number of iPS colonies compared with jnkwildtype MEFs. Overall, our results demonstrated that JNK1 and 2 play a negative role in reprogramming to pluripotent stem cells by suppressing Klf4 activity.
한우 고급육형 씨수소 정자의 액체질소 동결전후 운동성 및 생존성 비교
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.127
Effect of Aromatase on Sperm Viability, Penetration and Embryo Cleavage Rate in Pig
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.128
Characterization of Porcine Cryptorchidism Testes
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.129
CD14 is a Putative Marker for Porcine Spermatogonial Germ Cells
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.130
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