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Iron Depletion Decreases ROS in Porcine Early Embryos Developing In Vitro

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 38 No 2 Supplement (2014.06)바로가기
  • 페이지
    pp.106-106
  • 저자
    Ming-Hui Zhao, Shuang Liang, Jung-Woo Kwon, Seon-Hyang Kim, Nam-Hyung Kim, Xiang-Shun Cui
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A227344

원문정보

초록

영어
Iron is an important element which takes part in several biological processes. The role of iron in porcine oocyte maturation and early embryo development was still unknown. In the present study, iron was depleted by deferoxamine (DFM), a specific iron chelator, during in vitro maturation (IVM) and in vitro culture (IVC) period respectively. Results showed that, oocytes were matured with 1 μM DFM for 44 h followed by parthenogenetic activation (PA) displayed higher (p<0.05) blastocyst rate compared to without DFM in IVM medium. Another hand, supplemented with 0.5 μM DFM to the IVC medium for 7 days enhanced blastocyst formation both in PA and in vitro fertilization (IVF) groups (p<0.05). Although there was no difference in total cell number in IVF-derived blastocysts between control and iron depleted group, apoptotic index was significantly (p<0.05) decreased following iron depletion. ROS content in MII oocyte and blastocyst was significantly (p<0.05) decreased after iron depletion. For clarify the mechanism of the phenomenon, expressions of apoptotic- and ROS-related genes in the blastocyst were checked. Iron depletion reduced the expression of Caspase 3 and increased Bcl-xL in blastocyst (p<0.05), but there was no significanly differenc in m-RNA expression of Nox4. Mitochondrial membrane potential was increased both in MII oocytes and blastocysts after DFM treatment. In conclusion, iron depletion decreased ROS in oocytes and embryos developing in vitro, further enhanced mitochondrial activity and reduced the apoptosis in blastosyst, resulted in enhancement of the developmental capacity in porcine early embryos.

키워드

Iron Porcine Maturation Early embryo ROS

저자

  • Ming-Hui Zhao [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
  • Shuang Liang [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
  • Jung-Woo Kwon [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
  • Seon-Hyang Kim [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
  • Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]
  • Xiang-Shun Cui [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 center for Bio-Resource Development, Chungbuk National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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