Earticle

C57BL/6 마우스에서 자외선(Ultraviolet, UV) B 조사에 의한 표피 멜라닌세포의 변화에 대한 보중익기탕(Bu-Zhong-Yi-Qi-Tang, BZYQT)의 효과를 관찰하였다. 마우스에 UVB를 매일 80 mJ/cm2 (0.5 mW/sec)씩 7일간 조사하고, BZYQT를 UV 조사 전 또는 조사 후에 복강내주사 또는 피부에 도포하여, 멜라닌세포 형성 억제효과 및 형성된 멜라닌세포에 대한 미백 효과를 dihydroxyphenylalanine (DOPA) 염색으로 관찰하였다. 마우스의 귀등쪽 표피를 분리하여 관찰한바, 정상대조군에서는 mm2당 11-16개의 멜라닌세포가 관찰되었으며, UV 조사 일주일 후 발달된 가지를 가진 DOPA 양성 멜라닌세포는 급격히 증가하였다. 멜라닌세포 형성 억제 실험에서는 평균치를 기준으로 복강 내 주사군에서 16.3%, 피부도포군에서 26.6%(p<0.01)의 효과가 관찰되었으며, 형성된 멜라닌세포의 감소 효과 실험에서는 평균치를 기준으로 복강 내 주사군의 경우 3주에 24.0%(p<0.01), 6주에 26.0%(p<0.01)의 효과가 관찰되었고, 피부도포군의 경우 3주에 5.2%, 6주에 12.5%(p<0.05)의 효과를 보였다. 이상의 결과는 BZYQT가 UV에 의한 멜라닌세포 형성 억제제 및 미백제로서의 적용 가능성을 제시하였다.

We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of Bu-Zhong-Yi-Qi-Tang (BZYQT) on the formation, and decrease of UVB-induced epidermal melanocytes. C57BL/6 mice were irradiated by UVB 80 mJ/cm2 (0.5 mW/sec) daily for 7 days, and BZYQT was intraperitoneally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 melanocytes/mm2, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with BZYQT before each irradiation interrupted UVBinduced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with BZYQT at 3rd and 6th weeks after irradiation. The present study suggests the BZYQT as inhibitor of UVB-induced pigmentation and depigmenting agent.

The purpose of this study reported here was to investigate the ability of Bu-Zhong-Yi-Qi-Tang (BZYQT), known to elevate hematopoietic functions, to protect mice undergoing treatment with whole body single gamma-irradiation. BZYQT was given (25 mg/kg B.W.) intraperitoneally at 36 and 12 hours before irradiation and 30 minute and 24 hours after irradiation. Recovery of neutrophil and lymphocyte counts was significantly stimulated by extract of BZYQT. Stimulated recovery by the extract from the BZYQT was also observed in thrombocyte. However, the anti-radiation effect of erythrocyte, hemoglobin and hematocrit was not as significant as that of leukocyte. Further studies are needed to better characterize the protective nature of BZYQT extract and its ingredients.

암 환자를 대상으로 방사선 치료빈도가 높아짐에 따라 다양한 작용 기전을 바탕으로 한 방사선 방어제 개발에 많은 노력을 경 주하고 있다. 본 연구에서는 조혈, 면역세포의 증식과 다양한 싸이토카인의 분비를 증가시킴으로써 방사선 방어효능이 보고된 바 있는 인삼 다당체를 대상으로 화학적 구성성분과 각종 면역활성 지표 및 방사선 방어효능을 확인하고 가장 상관관계가 좋은 인자를 추출해내고자 하였다. 면역학적 활성으로는 임파구 증식능, 대식세포의 산화질소 분비능, AK세포 활성능을 검사하였으며 방사선 방어효과는 endogenous colony-forming unit(e-CFU)로 측정하였다. 인삼으로부터 추출된 다당체의 구성성분 중 galactose함량이 증가할수록 면역증강작용이 우수하였으며 임파구 증식능이 방사선 방어효과와 밀접한 관계가 있는 것으로 나타났다.

According to the increase in the use of radiotherapy to cancer patients, many approaches have been tried to develop new agents for the protection of surrounding normal tissues. However, it is still few applied in the clinic as a radioprotector. We aim to find a representative parameter for radioprotection to easily predict the activity of in vivo experiment from the results of in vitro screening. The polysaccharide extracted from Panax ginseng was used in this study because the immunostimulator has been regarded as one of the radioprotective agent category and was already reported having a promising radioprotective activity through the increase of hematopoietic cells and the production of several cytokines. Mitogenic activity, AK cells activity and nitric oxide production were monitored for the in vitro immunological assay, and endogenous colony-forming unit (e-CFU) was measured as in vivo radioprotective parameter. The immunological activity was increased by the galactose contents of ginseng polysaccharide dependently. The result of this study suggests that mitogenic activity of splenocytes demonstrated a good correlation with in vivo radioprotective effect, and may be used as a representative parameter to screen the candidates for radioprotector.

감자의 재배기간 중에 침적된 방사성 핵종의 토양-작물체 전이계수(TFa, m2 kg-1-fresh)를 측정하기 위하여 감자의 파종 2 일전 및 생육 중 세 차례에 걸쳐 54Mn, 60Co, 85Sr, 137Cs 함유 용액을 재배상자의 토양표면에 처리하였다. 파종 전 처리에서는 핵종이 표토(pH 5.2의 양질사토)와 혼합되었다. 조사 부위는 잎, 줄기, 괴경껍질, 괴경육이었다. 파종 전 처리시 54Mn, 60Co, 85Sr, 137Cs의 TFa 값은 작물체 부위에 따라 각각 1.9×10-4~1.5×10-2, 1.8×10-4~7.5×10-4, 4.0×10-4~1.6×10-2, 1.5×10-4~3.9×10-4의 변이를 보였다. 생육 중 처리시 TFa 값은 파종 전 처리에 비해 대체로 수 배 정도 낮았다. 54Mn, 85Sr, 137Cs의 경우 생육초기 또는 중기 처리시 후기 처리보다 높았으나 60Co의 경우 이와 반대였다. 부위 간에는 대체로 잎에서 가장 높았고 괴경육에서 가장 낮았다. 토양으로부터 네 부위로의 총 흡수율은 0.05~3.16%의 범위였다. 파종 전 처리 후 3 년차 54Mn, 60Co, 137Cs의 TFa 값은 부위에 따라 각각 1년차의 11~25%, 21~25%, 38~67%였다. 본 연구결과는 감자의 생육 중 방사성 핵종의 단기침 적시 영향을 예측하거나 관련 모델의 검증에 활용할 수 있다.

To measure the soil-to-plant transfer factors (TFa, m2 kg-1-fresh) of radionuclides deposited during the growing season of potato, a radioactive solution containing 54Mn, 60Co, 85Sr and 137Cs was applied to the soil surfaces in soil boxes 2 d before seeding and three different times during the plant growth. For the pre-seeding application (PSA), radionuclides were mixed with the topsoil (loamy sand and 5.2 in pH). The plant parts investigated were leaves, stems, tuber skin and tuber flesh. The TFa values of 54Mn, 60Co, 85Sr and 137Cs from the PSA were in the ranges of 1.9 10-4~1.5 10-2, 1.8 10-4~7.5 10-4, 4.0 10-4~1.6 10-2, 1.5 10-4~3.9 10-4, respectively, for different plant parts. The TFa values from the growing-time applications were on the whole a few times lower than those from the PSA. For 54Mn, 85Sr and 137Cs, the TFa values from the early- or middle-growth-stage application were higher than those from the late-growth-stage application, whereas the opposite was true for 60Co. Leaves and tuber flesh had the highest and lowest TFa values, respectively, in most cases. The total uptake from soil by the four plant parts was in the range of 0.05~3.16%. In the third year following the PSA, the TFa values of 54Mn, 60Co and 137Cs were 11~25%, 21~25% and 38~67% of those in the first year, respectively, depending on the plant parts. The present results can be used for estimating the radiological impact of an acute radioactive deposition during the growing season of potato and for testing the validity of relevant food-chain models.

이 연구에서는 국내 개발중인 초고온가스로 (VHTR: Very High Temperature Reactor)를 대상으로, 발생되는 삼중수소 양, 계통간 이송, 제거, 분포 그리고 최종적으로 생산된 수소에 대한 삼중수소에 의한 오염 준위를 예측할 수 있는 해석 모델인 TRITGO 코드를 소개하였고, 수소를 생산하는 IS (Iodine Sulfide) 계통으로의 삼중수소 투과양을 모의할 수 있도록 코드를 개선하였다. 또한 GT-MHR 600MW 열출력을 가정, 최종 수소 생산물의 삼중수소에 의한 오염치를 예측하였다. 예상 오염치는 약 0.055 Bq/H2-g 으로 일본 규제치 56 Bq/H2-g 에 약 1/1000 수준으로 낮게 예측되었다. 모의 결과 삼중수소 방출을 억제하기 위해서는 피복관의 건전성 유지 및 헬륨 냉각재와 흑연으로 구성된 반사체내 불순물인 3He 및 Li을 가능한한 낮은 준위로 유지하는 것이 필요함을 보여 주었다. 또한 냉각재내 불순물을 직접 제거할 수 있는 정화계통의 성능이 중요한 설계인자로 판단되었다.

In this study, The TRITGO code was introduced, which can predict the amount of tritium production, it's transport, removal, distribution and the level of contamination for the produced hydrogen by the tritium on the VHTR (very high temperature gas cooled reactor). The TRITGO code was improved so that the permeation to the IS Iodine Sulfide) loop for producing the hydrogen can be simulated. The contamination level of the produced hydrogen by the tritium was predicted by the improved code for the VHTR with 600MW thermal power. The contamination level for the produced hydrogen by tritium was predicted as 0.055 Bq/H2-g. This level is three order of lower than the regulation value of 56 Bq/H2-g from Japan. From this study, the following results were obtained. it is important that the fuel coating (SiC layer) should be kept intact to prevent the tritium from releasing. Also it is necessary that the level of impurity such as 3He and Li in the helium coolant and the reflector consisting of the graphite should be kept as low as possible. It was found that the capacity of the purification system for filtering the impurities directly from the coolant will be the important design parameter.

한국원자력연구원 새빛연료과학동의 굴뚝에서 대기로 방출되는 배기의 방사능을 측정, 분석 및 평가하였다. 동 시설에서는 연구용원자로 하나로의 핵연료를 생산하는 외에 첨단 핵연료를 연구하고 있으며 환경을 보호하기 위하여 시설외부로 방출되는 굴뚝배기 방사능 감시기를 연속 가동하고 있다. 2008년 1월 ~ 3월동안 굴뚝배기 방사능 감시기의 밀리포어 집진필터에 포집된 방 사능과 핵종을 정기적으로 측정하고 핵종을 분석한 결과, 천연방사성 핵종인 라돈(222Rn)과 토론(220Rn)의 단 반감기의 딸핵종들 및 40K이 미량으로 검출되었으나 72시간 이내에 계측기의 최소검출방사능(MDA) 이하로 붕괴하였으며 우라늄 핵종은 검출되지 않았다. 이로서 한국원자력연구원 새빛연료과학동으로부터 우라늄 핵종은 대기중으로 방출되지 않는 것으로 평가되었다.

Radioactivities of the stack air effluents from the Advance Fuel Science Building (AFSB) at KAERI have been investigated and evaluated. In this AFSB, nuclear fuels for the HANARO research reactor have been fabricated and the advanced nuclear fuels have been studied. A stack air monitoring system has been continuously operating to monitor the stack air effluents from the facility to protect the environment. As the results of the periodical radioactivity measurement and both the gamma and alpha spectrometry for the millipore filters taken from the stack air monitor from January until March 2008, a trace amount of primordial 40K and the short-lived decay products of natural borne 222Rn and 220Rn have been detected. However, the radioactivities have rapidly decayed to the level below the Minimum Detectable Activity (MDA) of the counting system. Therefore, it was evaluated that no uranium isotopes have been released to the atmosphere from the stack of the AFSB at KAERI.