발생공학 국제심포지엄 및 학술대회 The 13th International Symposium on Developmental Biotechnology::한국동물생명공학회(구 한국동물번식학회)

To investigate the efficient laboratory techniques for the injection of TALENs mRNA into porcine parthenogenesis and IVF embryos we had explores the injection time, volume and concentration, for the efficient blastocyst production has been identified within the experimental period. In experiment 1, to investigate injection time, compared 4 different time durations (1 hr, 2 hrs, 4 hrs & 5 hrs) after post activation of parthenogenetic embryos. There is no significant difference between four groups regarding the percentage of cleavages (55%). But comparatively higher percentage (20%) of blastocyst can be observed in the groups of 1hr and 2hrs injection after post activation. Even though observed higher blastocysts, only EGFP expressed blastocysts (2.2%) taken from the treatment of injection after 4 hrs of post activation. In experiment 2, compared two different concentration of EGFP injection in to embryos. 20 ng/μL and 50 ng/μL two concentrations were injected to the parthenogenetic embryos after 4 hrs of post activation and observed blastocysts after 7 days of incubation. There were significant difference between two treatments of concentrations considering percentages of cleavages and blastocysts. 50 ng/μL concentration injected embryos capable enough to cleaved up to 39% and produces blastocysts 10%, meanwhile 20 ng/ μL concentration illustrates 62% cleavage and 16% blastocysts percentages. Only 20 ng/μL injected embryos capable enough to express EGFP (3.1%) after 7 days of incubation. These results implies that 20 ng/μL concentration and injection 4hrs after post activation more effective injection conditions for microinjection.

145

hEPO 유전자가 발현되는 형질전환 닭의 생산

구본철, 권모선, 노지열, 김민지, 김태완

한국동물생명공학회(구 한국동물번식학회) 발생공학 국제심포지엄 및 학술대회 Vision of Animal Reproductive Biotechnology ; from Basic Research to Practical Applications 2013.10 p.195

146

No Porcine Endogenous Retrovirus (PERV) Elements Expression after Pig-to-Nonhuman Primate Xenotransplantation

Seongsoo Hwang, Yideun Jung, Kahee Cho, Keon Bong Oh, Sun-A Ock, Seoki Im, Heui-Soo Kim, Ik Jin Yun, Curie Ahn, Jin-Ki Park

한국동물생명공학회(구 한국동물번식학회) 발생공학 국제심포지엄 및 학술대회 Vision of Animal Reproductive Biotechnology ; from Basic Research to Practical Applications 2013.10 p.196

147

Erythropoietin Inhibits Gluconeogenesis and Improves Glucose Tolerance in EPO Transgenic Pig

Sun-young Baek, Won-kil Lee, Soo-hyne Park, Mi-Ryung Park, Kyung-Woon Kim, Jin-Ki Park, Sung-Ku Hong, Hak-Jae Chung

한국동물생명공학회(구 한국동물번식학회) 발생공학 국제심포지엄 및 학술대회 Vision of Animal Reproductive Biotechnology ; from Basic Research to Practical Applications 2013.10 p.197

148

Establishment of hDAF Knock-in Vector for Xenotransplantation

Jung Taek Kang, A Rum Park, Eun Jin Lee, Young Chul Par, Yun Jin Yun, Dal Young Ji, Kwang Wook Park

한국동물생명공학회(구 한국동물번식학회) 발생공학 국제심포지엄 및 학술대회 Vision of Animal Reproductive Biotechnology ; from Basic Research to Practical Applications 2013.10 p.198

149

Production of Living Piglet using 4 Genes Multi-Cistronic Vector for the Generation of Alzheimer's Disease Animal Model

Hyun-Yong Choi, Eun-Young Kim, Seung-Eun Lee, Jeremiah Ji-Man Moon, Min-Jee Park, Mi-Ryung Park, Hak-Jae Park, Jin-Ki Park, Se-Pill Park

한국동물생명공학회(구 한국동물번식학회) 발생공학 국제심포지엄 및 학술대회 Vision of Animal Reproductive Biotechnology ; from Basic Research to Practical Applications 2013.10 p.199

Earticle

Issues

발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

Sperm Male Reproduction

Stem Cells

Transgenesis Xenotranplantation