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Surface Markerand Paracrine Factors based Neural Differentiation of Male Germline Stem Cells
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.136
ERK1 Phosphorylates iPS Factor to Regulate Protein Stability and Stem Cell Self-Renewal
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.137
Nanogregulates human and mouse embryonic stem (ES) cell self-renewal activity. Activation of ERKs signaling negatively regulates ES cell self-renewal and induces differentiation, but the mechanisms are not understood. We found that ERK1 binds and phosphorylates Nanog. Activation of MEK/ERKs signaling and phosphorylation of Nanog inhibit Nanog transactivation, inducing ES cell differentiation. Conversely, suppression of MEK/ERKs signaling enhances Nanog transactivation to inhibit ES cell differentiation. We observed that phosphorylation of Nanog by ERK1 decreases Nanog stability through ubiquitination-mediated protein degradation. Further, we found that this phosphorylation induces binding of FBXW8 with Nanog to reduce Nanog protein stability. Overall, our results demonstrated that ERKs-mediated Nanog phosphorylation plays an important role in self-renewal of ES cells through FBXW8-mediated Nanog protein stability.
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 pp.138-139
Culture of Cryopreserved Spermatogonia for Long Term Maintenance
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.140
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.141
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.142
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.143
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.144
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.145
The Pluripotent Status of Porcine Embryonic Stem Cell Lines Derived from Various Origins
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.146
The pluripotent stem cells can be divided to naïve and primed state according to their pluripotent state. In this study, total 7 primed ESC lines were derived from porcine embryos of various origins, including in vitro fertilized (IVF), parthenogenetic activation (PA) and nuclear transfer (iPS-NT) from a donor cell with induced pluripotent stem cells (iPSC). To characterize ESCs, we performed several approaches including morphological observation, intensive alkaline phosphatase activity, the strongly pluripotency-associated genes such as POU5F1 (formerly known as OCT4), Sox2 and NANOG, immunocytochemistry analysis, the three germ layer associated genes such as AFP (edndoderm), DESMIN (mesoderm), CRABP2 (ectoderm), karyotyping and embryoid body formation. Furthermore, we investigated the XIST gene expression from all female cell lines. Establishment of porcine naïve ESCs, especially iPS-NT ESCs, will make possible sophisticated genetic manipulation to create ideal animal models for pre-clinical research and studies of human diseases. This study will help to approach the goal for establishing naive pluripotent stem cells in pigs.
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.147
Purification of the Human Tumor Nerosis Factor alpha Receptor in the Milk of Transgenic Pig
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.151
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.152
Functional Analysis of α-1,3-Galactosyltransferase Knock-out (—/—) Pigs
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.153
Efficient RGEN-Mediated Homologous Recombination in the Bovine Fibroblasts
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.154
돼지 단위발생 난자에서 CRISPR/Cas9 시스템을 이용한 FoxN1 유전 형질전환 효율 분석
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.155
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.156
The Role of Calcineurin Binding Protein 1 in Rheumatoid Arthritis
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.157
Improved Productivity of 11β-HSD1 Overexpressed Piglets by Re-Cloning
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.159
유선조직 특이적인 hEPO 유전자 발현의 In Vitro 검정
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.160
IL2RG Gene-Targeted Severe Combined Immunodeficiency Pigs
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.161
한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.162
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