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Reproductive & Developmental Biology(Supplement)

간행물 정보
  • 자료유형
    학술대회
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 간기
    연간
  • 수록기간
    2001 ~ 2017
  • 주제분류
    농수해양 > 축산학
  • 십진분류
    KDC 527 DDC 636
Volume 38 No 2 Supplement (114건)
No
92

Nanogregulates human and mouse embryonic stem (ES) cell self-renewal activity. Activation of ERKs signaling negatively regulates ES cell self-renewal and induces differentiation, but the mechanisms are not understood. We found that ERK1 binds and phosphorylates Nanog. Activation of MEK/ERKs signaling and phosphorylation of Nanog inhibit Nanog transactivation, inducing ES cell differentiation. Conversely, suppression of MEK/ERKs signaling enhances Nanog transactivation to inhibit ES cell differentiation. We observed that phosphorylation of Nanog by ERK1 decreases Nanog stability through ubiquitination-mediated protein degradation. Further, we found that this phosphorylation induces binding of FBXW8 with Nanog to reduce Nanog protein stability. Overall, our results demonstrated that ERKs-mediated Nanog phosphorylation plays an important role in self-renewal of ES cells through FBXW8-mediated Nanog protein stability.

100

The pluripotent stem cells can be divided to naïve and primed state according to their pluripotent state. In this study, total 7 primed ESC lines were derived from porcine embryos of various origins, including in vitro fertilized (IVF), parthenogenetic activation (PA) and nuclear transfer (iPS-NT) from a donor cell with induced pluripotent stem cells (iPSC). To characterize ESCs, we performed several approaches including morphological observation, intensive alkaline phosphatase activity, the strongly pluripotency-associated genes such as POU5F1 (formerly known as OCT4), Sox2 and NANOG, immunocytochemistry analysis, the three germ layer associated genes such as AFP (edndoderm), DESMIN (mesoderm), CRABP2 (ectoderm), karyotyping and embryoid body formation. Furthermore, we investigated the XIST gene expression from all female cell lines. Establishment of porcine naïve ESCs, especially iPS-NT ESCs, will make possible sophisticated genetic manipulation to create ideal animal models for pre-clinical research and studies of human diseases. This study will help to approach the goal for establishing naive pluripotent stem cells in pigs.

102

Purification of the Human Tumor Nerosis Factor alpha Receptor in the Milk of Transgenic Pig

Keun Jung Kim, Ji Hye Lee, Eun Young Kim, Kang Sun Park, Kil Woo Han, Jeong Yu, Jun Jong Baek, Jae Sam Lim, Hong Ju Kim, Hong Kyun Kim, Min Kyu Kim

한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.151

109

Improved Productivity of 11β-HSD1 Overexpressed Piglets by Re-Cloning

Yeon-Ik Jeong, Yubyeol Jeon, Chi-Hun Park, Kyeong-Hee Ko, Yeon-Woo Jeong, Yeun-Wook Kim, Sang-Hwan Hyun, Il Suk Yang, Woo-Suk Hwang

한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.159

111

IL2RG Gene-Targeted Severe Combined Immunodeficiency Pigs

Yun-Jung Choi, Deug-Nam Kwon, Woo-Jin Park, Jae-Woong Han, Min-Hui Kang, Da-som Kim, Eunsu Kim, Sangiliyandi Gurunathan, Jin-Hoi Kim

한국동물생명공학회(구 한국동물번식학회) Reproductive & Developmental Biology(Supplement) Volume 38 No 2 Supplement 2014.06 p.161

 
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