A novel hydrogen fermentation technique by using polyvinyl alcohol (PVA) gel beads as a biomass carrier was investigated. The hydrogen gas was stably produced throughout the experimental period in a continuous reactor. Even though the hydrogen productivity was suddenly decrease by experimental troubles, the bacteria attached to the PVA gel beads played as an inoculum, it was promptly recovered. The hydrogen yield per glucose was not very high (1.0 – 1.2mol-H2/mol-glucose), thus the optimization of the experimental conditions such as ORP and HRT should be considered to improve the hydrogen productivity. Bacterial community was stable during experimental period after the PVA gel beads applying, which indicated that applying of biomass carrier was specific to keep not only the biomass but also the bacteria commonly. Clostridium species were phylogenetically detected, which suggested that these bacteria contributed to the hydrogen production in the biofilm attached to the PVA gel beads..
목차
Abstract Introduction Materials and methods Continuous hydrogen fermentation Analysis Bacterial community analysis by denaturinggradient gel electrophoresis (DGGE) Results and Discussion Hydrogen productivity in PVA gel beads fluidized bed reactor Effect of PVA gel beads applying on hydrogen production and metabolites Relationships between hydrogen productionand lactic acid before and after applying PVAgel beads Results of bacterial community analysis by PCR-DGGE Conclusions Reference
키워드
Hydrogen fermentationPVA gel beadsFluidized bedBiofilmBacterial community
저자
Masaharu Nakao [ Graduate School of Science and Technology, Kumamoto University ]
Corresponding author
Yasunori Kawagoshi [ Graduate School of Science and Technology, Kumamoto University ]
Naoe Hino [ Graduate School of Science and Technology, Kumamoto University ]
Tomonori Iwasa [ Graduate School of Science and Technology, Kumamoto University ]
Kenji Furukawa [ Graduate School of Science and Technology, Kumamoto University ]