Earticle

초록

영어

Bacteria can sense and respond to diverse environmental signals, including chemical gradients, temperature shifts, osmotic stress, and mechanical forces. However, bacterial responses to complex acoustic environments remain poorly understood. In this exploratory study, we examined the phenotypic and metabolic characteristics of Escherichia coli exposed to two distinct acoustic conditions. We observed condition-associated differences in colony morphology, with the Chanyang condition showing greater biomass accumulation and more irregular and spreading colony structures than the K-pop and non-stimulated conditions. Because biofilm formation and extracellular matrix production were not directly measured, these morphological characteristics are interpreted as biofilm-like features rather than evidence of confirmed biofilm formation. To explore potential biological processes associated with these phenotypic differences, we combined untargeted metabolomics, molecular networking, and AI-assisted biological knowledge integration. The Chanyang-associated metabolic profile included several putatively annotated lipid-related features, including N-acylglycine-, N-acylethanolamine-, and ceramide-related features, as well as other metabolites potentially associated with membrane and stress-related processes. These annotations were not confirmed using authentic standards or orthogonal structural methods and therefore should be considered hypothesis-generating. The observed metabolic differences do not establish whether the response resulted from direct mechanosensory processes or indirect changes in the culture environment. Likewise, quorum-sensing activity was not directly assessed. Overall, the findings indicate that distinct acoustic exposure conditions were associated with differences in E. coli colony morphology and metabolic profiles. These observations provide a basis for future studies investigating how acoustic or mechanically associated environmental stimuli may influence bacterial physiology and cellular organization.

목차

Abstract
1. Introduction
2. Materials and Methods
2.1. Bacterial strains and culture conditions
2.2. Acoustic stimulation protocols
2.3. Metabolite extraction and sample preparation
2.4. LC-MS/MS profiling and data analysis
3. Results
3.1. Acoustic stimulation induces distinct morphological phenotypes
3.2. PLS-DA identified molecular features contributing to metabolic discrimination
3.3. Molecular networking revealed distinct molecular feature families
3.4. Putative N-acyl lipid features associated with the Chanyang condition
3.5. Additional putative respiratory- and membrane-associated features
4. Discussion
5. Conclusions
References

키워드

저자

• Ryoichi Yamada [ Department of Life Science and Biochemical Engineering, Sun Moon University/Genome-Based BioIT Convergence Institute, Asan 31460, Korea ]
• Hirotake Yamaguchi [ Department of Life Science and Biochemical Engineering, Sun Moon University/Genome-Based BioIT Convergence Institute, Asan 31460, Korea ]
• Tae-Jin Oh [ Department of Life Science and Biochemical Engineering, Sun Moon University/Genome-Based BioIT Convergence Institute/Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  The Journal of Sciences and Innovation for Sustainable Peace(구 The journal of Hyojeong Academia)

• 간기

  반년간

• pISSN

  3140-1619

• 수록기간

  2023~2026

• 십진분류

  KDC 238 DDC 289