Earticle

초록

영어

Purpose: In a previous study, two-spotted cricket oil (GBO) was characterized for terms of its fatty acid composition and its antioxidant and anti-inflammatory activities, while its antimicrobial activity remained unexamined. This study aimed to evaluate the antimicrobial activity of GBO against five animal pathogenic bacteria and to investigate the correlations among the antimicrobial, antioxidant, and anti-inflammatory activities of GBO. Methods: The antimicrobial activity of GBO was evaluated against five animal pathogens (Aeromonas salmonicida subsp. salmonicida, Staphylococcus aureus, Salmonella Typhimurium, Lactococcus garvieae, and Vibrio anguillarum) using the broth dilution method. Spearman correlation analysis was performed between the pathogen growth inhibition data and previously reported antioxidant, anti-inflammatory, and cell viability data for GBO. Results: GBO exhibited minimum inhibitory concentrations (MICs) ranging from 3.125% to 6.25% (v/v) against the five animal pathogenic bacteria, well below its previously established cytotoxic threshold. Bacterial growth was strongly negatively correlated with total flavonoid content (TFC) and DPPH radical scavenging activity, and strongly positively correlated with nitric oxide (NO) production. Conclusion: GBO exhibited broad-spectrum antimicrobial activity against all five pathogens tested and Spearman correlation analysis showed that this activity varied in a coordinated manner with its flavonoid-driven antioxidant capacity and suppression of NO production. These findings suggest an interconnected, rather than independent, basis for its antimicrobial, antioxidant, and anti-inflammatory properties and support its potential as a functional ingredient in veterinary cosmeceutical formulations for companion and aquatic animals.

한국어

목적: 우리는 이전 연구에서 쌍별귀뚜라미(Gryllus bimaculatus) 오일의 지방산 조성과 항산화, 항염증 기능성을 분석하였으나, 항 균 활성은 평가되지 않았다. 본 연구는 동물 병원성 세균 5종에 대한 쌍별귀뚜라미 오일의 항균 활성을 평가하고, 쌍별귀뚜라미 오 일의 항산화, 항염증, 항균 활성 간의 상관관계를 분석하고자 하였다. 방법: 쌍별귀뚜라미 오일의 항균 활성은 동물의 병원성 세균 5종(Aeromonas salmonicida subsp. salmonicida, Staphylococcus aureus, Salmonella Typhimurium, Lactococcus garvieae, Vibrio anguillarum)에 대해 액체배지 희석법을 이용해 평가되었다. 또한 본 연구에서 도출된 항균 활성과 기존에 보고된 항산화, 항염증 활성 간의 상관관계는 Spearman 분석을 통해 평가하였다. 결과: GBO는 5종의 병원성 세균 모두에서 농도의존적인 성장 억제 효과를 나타냈다. 최소억제농도(MIC)는 A. salmonicida, L. garvieae 및 V. anguillarum에서 3.125% (v/v), S. Typhimurium 및 S.aureus에서 6.25% (v/v)로 나타났다. 총 페놀 함량(TPC), 총 플라보노이드 함량(TFC) 및 DPPH 라디칼 소거능은 병원성 세균의 성장과 음의 상관관계를 나타냈으며, NO 생성은 병원성 세균의 성장과 양의 상관관계를 나타냈다. 결론: GBO는 본 연구에서 실험한5종 병원균 모두에 대해 항균 활성을 나타냈다. Spearman 상관분석 결과, 이러한 항균 활성은 항산화 지표 및 NO 생성 억제와 통계적으로 연관된 양상을 보였다. 이는 GBO의 항균, 항산화 및 항염증 기능성이 서로 독립적이기보다는 상호 연관되어 있음을 시사하며, 반려동물 및 관상어를 위한 코스메슈티컬 제형의 기능성 원료로 활용될 잠재력이 있음을 뒷받침한다.

중국어

目的: 在以往的研究中，双斑蟋蟀油(GBO)的脂肪酸组成及其抗氧化和抗炎活性已被表征，但其抗菌活性尚未经 审查。本研究旨在评估GBO对五种动物致病菌的抗菌活性，并探讨GBO的抗菌、抗氧化与抗炎活性之间的相关 性。方法: 采用肉汤稀释法评估了(GBO)对五种动物病原菌(杀鲑气单胞菌杀鲑亚种、金黄色葡萄球菌、鼠伤寒 沙门氏菌、鱼球菌和鳗弧菌)的抗菌活性。对病原菌生长抑制数据与此前报道的GBO抗氧化、抗炎和细胞活力 数据进行了斯皮尔曼相关性分析。结果: GBO对这五种动物致病菌表现出3.125%至6.25% (v/v)的最小抑菌浓度 (MIC)，远低于其此前确定的细胞毒性阈值。细菌生长与总黄酮含量(TFC)和DPPH自由基清除活性呈强负相关， 与一氧化氮(NO)生成量呈强正相关。结论: GBO对所有测试的五种病原菌均表现出广谱抗菌活性，斯皮尔曼相关 性分析表明，该活性与其黄酮驱动的抗氧化能力及NO生成抑制作用协调变化。这些发现表明其抗菌、抗氧化和 抗炎特性具有相互关联而非相互独立的依据，并支持其作为伴侣动物和水生动物兽用药妆配方中功能性成分的潜力。

목차

Abstract
Introduction
Materials and Methods
1. Processing and Composition of Two-Spotted Cricket Oil
2. Evaluation of Antimicrobial Activity
3. Statistical Analysis and Spearman Correlation
Results and Discussion
1. Antimicrobial Activity of GBO against Five Animal Pathogens
2. Correlations of Antimicrobial Activity with Antioxidant and Anti-inflammatory Properties of GBO
Conclusion
References
국문초록
참고문헌
中文摘要

키워드

저자

• Seung-Won Yi [ 이승원 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
• So-Young Lee [ 이소영 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
• Haesung Lee [ 이해성 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
• Joon Ha Lee [ 이준하 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
• Minchul Seo [ 서민철 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
• Ji Hae Lee [ 이지혜 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
• Heui-Sam Lee [ 이희삼 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
• You-Young Jo [ 조유영 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  아시안뷰티화장품학술지 [Asian Journal of Beauty and Cosmetology]

• 간기

  계간

• pISSN

  2466-2046

• eISSN

  2466-2054

• 수록기간

  2016~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 593 DDC 646