Earticle

초록

영어

Exercise-associated muscle cramps are thought to arise from impaired regulation of intracellular calcium homeostasis; however, the cellular mechanisms controlling aberrant calcium release remain incompletely defined. In this study, we utilized Dictyostelium discoideum , a conserved model for calcium signaling, to examine endoplasmic reticulum(ER)-derived calcium release and its modulation by magnesium, taurine, and caffeine. cAMP stimulation elicited a robust intracellular Ca2+ increase, which was suppressed by ER calcium store depletion, confirming the ER as the primary calcium release source. Extracellular magnesium inhibited Ca2+ release in a dose-dependent manner, with maximal responses observed in magnesium-free conditions and saturating inhibition at concentrations ≥ 0.5 mM; magnesium oxide exhibited a comparable inhibitory effect. Conversely, caffeine and taurine modestly reduced Ca2+ release amplitude while slightly accelerating the time-to-peak response, indicating fine-tuned modulation of ER calcium dynamics rather than complete inhibition. These findings demonstrate differential regulation of ER-mediated calcium release by widely used nutritional and pharmacological agents, providing cellular insights into calcium dysregulation underlying exercise-associated muscle cramp pathophysiology.

목차

Abstract
1. Introduction
1.1. Definition and clinical significance of muscle cramps
1.2. Types of muscle cramps
1.3. Rationale for focusing on exercise-associated muscle cramps(EAMC)
1.4. Mechanisms underlying muscle cramps
1.5. Integrated mechanism: neuromuscular dysregulation and disruption of calcium homeostasis
1.6. Calcium-dependent regulation of muscle contraction and mechanisms of ER-derived calcium release
1.7. Nutritional and pharmacological factors related to muscle cramps
1.8. Biological questions
2. Materials and Methods
2.1. Cell culture
2.2. Live-cell imaging and analysis of calcium dynamics in GCaMP3, 6-expressing cells
3. Results
4. Discussion
4.1. Primary source of cAMP-induced calcium release
4.2. Magnesium supports the possibility of ER calcium release through the direct action of magnesium ions.
4.3. Caffeine and taurine exert potent inhibition of ER calcium release
4.4. Implications and conclusions
Reference

키워드

저자

• Young Joon Hong [ Department of Biology, Chosun University, Gwangju 61452, Korea ]
• Hun Jeong [ Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University/Research Institute of Chosun Natural Science & CSU G-LAMP Project Group, Chosun University, Gwangju, 61452, Korea ]
• Taeck Joong Jeon [ Department of Biology, Chosun University/Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University/Research Institute of Chosun Natural Science & CSU G-LAMP Project Group, Chosun University ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  통합자연과학논문집(구 조선자연과학논문집) [Journal of Integrative Natural Science]

• 간기

  계간

• pISSN

  2005-1042

• 수록기간

  2008~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 405 DDC 505