Earticle

초록

영어

CCl4-induced liver injury is closely associated with oxidative stress-mediated impaired hepatic synthetic function, resulting in coagulation disorders; however, effective dietary interventions remains limited. This study aimed to investigate the hepatoprotective and anticoagulant effects of quercetin and rutin, bioflavonoids from Tartary buckwheat (Fagopyrum tartaricum) in a CCl4-induced rat model. Sprague-Dawley rats were randomly divided into six groups (n = 8 per group, total n = 48): Normal, Quercetin, Rutin, CCl4, CCl4 + Quercetin, CCl4 + Rutin. The rats were orally administered either vehicle, rutin, or quercetin (300 mg/kg) once daily for five days. Acute hepatic injury was induced by a single intraperitoneal injection of CCl4 (25% in olvie oil, 4 mL/kg) on the final days. Rats in the pre-treatment groups were administered rutin or quercetin prior to CCl4 exposure. Statistical analysis was performed using one-way ANOVA, and statistical significance was set at p < 0.05. Biochemical and coagulation parameters were assessed and, also histopathological changes of liver sections were examined. In the CCl4 group, liver enzyme levels (AST, ALT, and ALP) were significantly elevated compared to the normal group (F= 213.27, 89.34, and 44.33, respectively; all p < 0.001). Notably, while both quercetin and rutin significantly mitigated these increases, rutin exhibited a superior restorative effect on AST level (313.25 ± 32.61 IU/L) compared to quercetin (360.12 ± 41.61 IU/L) (p < 0.05). Similarly, while rutin showed a slightly more potent restorative effect on coagulation parameters than quercetin, the difference between the two groups did not reach statistical significance (p > 0.05). Histopathological results supported the serum biochemical evidence, revealing that rutin-pretreated liver tissues showed more improved morphological features compared to the quercetin group. These results suggest that quercetin and rutin have therapeutic potential against CCl4-associatedcoagulationabnormalitiesandconsequentialliverdamageinrats.

한국어

본 연구는 랫드에서 퀘르세틴과 루틴의 사전투여가 CCl4로 유발된 급성 간 손상에 미치는 보호효과를 평가하고자 수행 되었다. 퀘르세틴과 루틴은 체중 1 kg 당 300 mg씩 하루 한 번, 5일간 경구 투여하였다. 마지막 투여 24시간 후 CCl4 (4 mL/kg, 1:4 in olive oil)를 정맥 주사하여 간 손상을 유도하고, 혈청에서 AST, ALT, ALP 농도와 항응고 활성 (PT 및 aPTT)을 검사하였다. 실험결과, 퀘르세틴과 루틴의 투여는 CCl4로 인해 상승한 ALT, ALT 및 ALP 수치를 유의하게 억제하였 으며(p < 0.05), 증가한 항응고 활성과 조직학적 손상 역시 유의하게 완화되었다. 이상의 결과는 퀘르세틴과 루틴의 사전 투여 가 혈액 응고 장애를 포함한 급성 간 손상을 예방할 수 있음을 알 수 있었다.

목차

Abstract
요약
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Animal
2.3. Experimental design
2.4. Preparation of blood sample
2.5. Measurement of serum biochemcial parameters
2.6. Measurement of coagulation parameters
2.7. Tissue collection and histological assays
2.8. Statistical analysis
3. Results and Discussion
3.1. Effect of quercetin and rutin on biochemical parameters
3.2. Effect of quercetin and rutin on coagulation parameters
3.3. Histopathology
4. Conclusion
REFERENCES

키워드

메밀 루틴 퀘르세틴 사염화탄소 간 보호효능 혈액응고 장애 Tartary buckwheat rutin quercetin CCl4 hepatoprotective coagulation disturbance

저자

• Kwang Yeon Lee [ 이광연 | Researcher, Dept. of Food and Nutrition, Far East University ]
• In Young Bae [ 배인영 | Professor, Dept. of Food and Nutrition, Far East University ] Corresponding Author

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