This study is reported the test results of clinical efficacy on the skin through human application experiments with serum and cream manufactured using Nano-UA60. As a result of the UA toxicity test through cell culture, cells showed activity when the concentration of UA was 0.6 μM or less, and toxicity was found above 1.2 μM. As a result of testing with the skin patch test with UA-cream, 0.1% of SLS is shown a severe irritation of 31.25, and 5,000 IU/g RP showed a mild irritation of 11.25. 1.0% Nano-UA60 showed minimal stimulation at 6.3. 0.1-1.0% UA-serum showed lower stimulation than 0.1% SLS and 5000 IU/g RP. The collagen synthesis rate is shown 0.2% activity in control, a comparative group, but in the case of UA, it showed a proliferation effect of 17.0% at 1 μM and 23.6% at 2 μM. The elastin synthesis rate is shown a low activity of 1.2% in control, but in the case of UA, it showed a proliferation effect of 41.9% at 1 μM. The inhibitory effect of elastase according to the content change of UA was 7% at 4 ppm, 40% at 10 ppm, 80% at 20 ppm, and 86% at 40 ppm. The wrinkle improvement effect evaluated by the researcher had a significant effect compared to plasebo in thick wrinkles, thin wrinkles, left cheek areas, and skin roughness. Moisturizing power before application was 55.7, and based on this, the skin moisturizing effect after 90 minutes of sample application increased by 53.1% and 56.1% compared to before application. The moisturizing power of Nano-UA60 serum after 2 hours was 98.8, which was 14.3% higher than that of Placebo, and the moisturizing power after 8 hours was 80.08, which was higher than that of Placebo. Through the above results, the ursolic acid component is expected to be widely used the high functional cosmetics fields also to the pharmaceutical industry as an excellent component with a fine wrinkle improvement effect.
목차
Abstract Ⅰ. 서 론 Ⅱ. 실험 및 연구방법 1. 실험재료 2. 실험장치 Ⅲ. 실험방법 1. 나노-UA60,크림과 세럼의 제조방법 2. UA의 세포독성시험법 3. 효능효과 평가방법 Ⅳ. 결과 및 고찰 1. 나노 니오좀 캡슐의 구조확인 및 입경분포 측정결과 2. Normal Human Fibroblast를 사용한 UA 세포독성과 세럼의 피부안전성 3. 콜라겐 단백질 합성률 4. 피부잔주름개선 효과 5. 피부보습 효능효과 결과 Ⅴ. 결론 References
K-뷰티인그리에경영학회(구 한국인그리에학회) [The Society of K-Beauty Ingrier Management]
설립연도
2018
분야
예술체육>미용
소개
인그리에(Ingrier)란 화장품 성분, 제형, 향, 칼라, 느낌 뿐만 아니라 유효성, 안전성 등을 감별하여 고객의 기호에 맞게 화장품을 추천하여 적합한 사용법을 제공해 고객의 만족을 이끌어 낼 수 있는 화장품 전문가를 말합니다. 본 한국인그리에학회는 대학, 기업 및 다양한 분야의 현장에서 화장품 산업의 발전을 기대하며 뷰티융합의 장을 열어보고자 합니다. 아울러 인그리에가 성장할 수 있는 각 분야의 우수한 연구를 확장하고 선도하는 수준 높은 학술지로서의 역할을 다하고자 합니다.
간행물
간행물명
한국인그리에학회지 [Journal of Korea Society of Ingrielogy]