Earticle

초록

영어

Purpose: The purpose of this study was to determine the potential of Achyranthes fauriei as a functional ingredient by analyzing its physiological activity and identifying its main components. Methods: Radical scavenging activity was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. Total polyphenol and flavonoid content in A. fauriei root extracts were measured. The concentrations of each component in the samples were measured by HPLC. For quantitative analysis, the standard substances, 20-hydroxyecdysone and 25S-inokosterone, were used to compare and analyze the calibration of these two compounds. Results: The DPPH radical scavenging activity of Achyranthes fauriei root extracts was increased in the order AFR-100 E>AFR-50 E>AFR-D.W., based on their concentration. The ABTS assay revealed an increase in the order AFR-50 E>AFR-100 E>AFR-D.W., based on concentration. The total polyphenol content was in the order AFR-100 E>AFR-50 E>AFR-D.W.; total flavonoid content followed the same trend. HPLC analysis revealed that 20-hydroxyecdysone had the highest concentration in the AFR-50 E sample, whereas 25S-inokosterone had the highest concentration in the AFR-100 E sample. Conclusion: Based on in vitro studies of the A. fauriei root, a calibration analysis of 20-hydroxyecdysone and 25S-inokosterone revealed very high correlation coefficients, indicating a strong linear relationship as concentration increased, with a significant increase in flavonoid content. The results indicate that A. fauriei root contains a high number of flavonoids, which are natural compounds exhibiting excellent antioxidant and anti-inflammatory effects. We also established a robust quantitative analysis method for A. fauriei.

한국어

목적: 본 연구는 기능성 원료로서의 우슬의 생리적 활성 능력과 주요성분의 함량을 분석하여, 우슬의 기능성 원료로서의 가능 성을 탐색하고자 하였다. 방법: DPPH 및 ABTS 실험을 통해 라디칼 소거 활성을 분석하였고, 우슬 추출물의 총 폴리페놀 및 총 플라보노이드 함량을 분석하였다. HPLC 분석을 통해 시료 내 각 성분의 농도를 정밀하게 측정하고 정량 분석을 위해 표준 물질 20-hydroxyecdysone와 25S-inokosterone를 두 화합물의 검량을 비교 분석하였다. 결과: Achyranthes fauriei 뿌리 추 출물의 DPPH 라디칼 제거 활성 실험 결과 AFR-100 E>AFR-50 E>AFR-D.W. 순으로 농도 의존적으로 증가하였다. ABTS 분석결과 AFR-50 E>AFR-100 E>AFR-D.W. 순으로 농도 의존적으로 높아졌다. 총 폴리페놀 함량의 결과는 AFR-100 E>AFR-50 E>AFR-D.W. 순이었으며, 총 플라보노이드 함량 AFR-100 E>AFR-50 E>AFR-D.W. 순이었다. HPLC 분석결과 20-hydroxyecdysone는 AFR-50 E 샘플에서 가장 높은 농도를, 25S-inokosterone는 AFR-100 E 샘플에서 가장 높은 농도를 보 였다. 결론: 본 우슬의 in vitro 실험을 통해 20-hydroxyecdysone와 25S-inokosterone 검량분석결과, 두 그래프 모두 상관계수가 매우 높아 농도가 클수록 데이터의 선형 관계가 크며, 플라보노이드 함량이 크게 증가할 수 있음을 확인하였다. 본 연구 결과 우슬 의 뿌리가 항산화와 항염 효과가 뛰어난 천연 화합물인 플라보노이드를 다량 포함하고 있음을 확인하였으며, 우슬의 우수한 정량분 석 방법을 제시하였다.

중국어

目的: 通过分析牛膝的生理活性并鉴定其主要成分，以确定牛膝作为功能成分的潜力。方法: 通过DPPH和ABTS 实验分析自由基清除活性，并分析牛膝提取物的总多酚和总黄酮含量。通过HPLC分析，精确测定样品中各成分 的浓度，并采用标准物质20-羟基蜕皮酮和25S-肌酮进行定量分析，对两种化合物的校准进行比较分析。结果: 牛膝根提取物的DPPH自由基清除活性根据其浓度按 AFR-100 E>AFR-50 E>AFR-D.W.的顺序增加。ABTS 测定 显示，根据浓度AFR-50 E>AFR-100 E>AFR-D.W.的顺序增加。总多酚含量顺序为AFR-100 E>AFR-50 E>AFR-D. W.；总黄酮含量也呈现同样的趋势。HPLC分析显示，20-羟基蜕皮酮在AFR-50 E样品中浓度最高，而25S-肌诺 酮在AFR-100 E样品中浓度最高。结论: 基于对牛膝根的体外研究，20-羟基蜕皮酮和25S-肌睾酮的校准分析显示 出非常高的相关系数，表明随着浓度的增加呈很强的线性关系，黄酮类化合物含量显着增加。结果表明，牛膝 根含有大量黄酮类化合物，它们是具有优异抗氧化和抗炎作用的天然化合物，并提出了一种优良的植物定量分 析方法。

목차

Abstract
Introduction
Methods
1. Sample preparation
2. Statistical processing
3. In vitro assay
Results and Discussion
1. Extraction results by solvent
2. DPPH radical scavenging activity
3. ABTS radical scavenging activity
4. Total polyphenol content
5. Total flavonoid content
6. HPLC contents analysis
Conclusion
Acknowledgements
Author's contribution
Author details
References
국문초록
참고문헌
中文摘要

키워드

저자

• Joon Han Lee [ 이준한 | School of Industrial Bio-Pharmaceutical Science, College of Health & Biotechnology, Semyung University, Jecheon-si, Chungcheongbuk-do, Korea ]
• Do Hyeong Kim [ 김도형 | Department of Oriental Medical Food & Nutrition, College of Health & Biotechnology Semyung University, Jecheon-si, Chungcheongbuk-do, Korea ]
• Jin Jung Ahn [ 안진정 | Department of Beauty Care, College of Health & Biotechnology, Semyung University, Jecheon-si, Chungcheongbuk-do, Korea ] Corresponding Author
• Byung Wook Yang [ 양병욱 | School of Industrial Bio-Pharmaceutical Science, College of Health & Biotechnology, Semyung University, Jecheon-si, Chungcheongbuk-do, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  아시안뷰티화장품학술지 [Asian Journal of Beauty and Cosmetology]

• 간기

  계간

• pISSN

  2466-2046

• eISSN

  2466-2054

• 수록기간

  2016~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 593 DDC 646