Earticle

초록

영어

This study aimed to explore the potential application of Salix gracilistyla leaf extract to the skin, evaluating its antioxidant effects and protective properties against oxidative stress using human-derived keratinocytes. The findings revealed that the extract exhibited significant DPPH radical scavenging activity with an IC50 value of 109.29± μg/mL, indicating a dose-dependent increase in scavenging activity. Furthermore, the extract contained notable levels of total polyphenols (203.13±3.25 μg/mL) and flavonoids (154.31±1.16 μg/mL). No cytotoxicity was observed in HaCaT cells at concentrations below 100 μg/mL. Additionally, the extract demonstrated dose-dependent protective effects against hydrogen peroxide-induced oxidative stress. These results imply that Salix gracilistyla leaf extract holds promise for antioxidant skincare applications, potentially mitigating harmful various factors that can damage the skin. Further research in this area is expected to deepen our understanding of its benefits and applications.

중국어

本研究旨在于确认藤柳叶提取物的抗氧化效果及其对皮肤的适用性，本研究利用藤柳叶提取物的抗氧化作用及人源皮肤角质形成细胞，测定了其细胞毒性。研究结果表明，DPPH自由基清除能力IC50值为109.29±μg/mL，清除能力依赖浓度呈现出增加趋势。总多酚含量为203.13±3.25μg/mL，黄酮含量为154.31±1.16μg/mL。HaCaT角质细胞的细胞毒性在浓度为100μg/ml以下时，未呈现出细胞毒性，过氧化氢引起的对氧化压力的保护作用则依赖其浓度呈现出显著的保护效果。基于上述结果，可以推断藤柳叶提取物对皮肤具有抗氧化效果，并能够保护及消除皮肤有害因素。因此，今后需要对此进行持续的研究。

목차

Abstract
I. 서론
II. 이론적 배경
1. 항산화 효과
2. 각질세포
III. 연구방법
1. 재료 및 추출 방법
2. 총 폴리페놀 함량
3. 총 플라보노이드 함량
4. DPPH 라디칼 소거 활성
5. 세포배양
6. 세포 생존율 측정
7. HaCaT 세포에서 H2O2에 의해 유도된 산화적 스트레스에 대한 세포 보호효과 측정
8. 통계처리
III. 결과 및 고찰
1. 총 폴리페놀과 플라보노이드 함량
2. DPPH radical 소거활성 측정
3. 세포 생존율에 미치는 영향
4. HaCaT 세포에서 H2O2에 의해 유도된 산화적 스트레스에 대한 세포 보호 효과
IV. 결론
참고문헌
中文摘要

키워드

저자

• 박주아 [ Ju A Park | 청암대학교 향장피부미용과 교수 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  국제보건미용학회지 [Journal of Health and Beauty]

• 간기

  계간

• pISSN

  2005-369X

• eISSN

  2765-1347

• 수록기간

  2007~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 593 DDC 646