Earticle

초록

영어

Kluyveromyces marxianus 17555-DF-33 was engineered by integration of KmXYL1 and KmXYL2 genes into K. marxianus 17555ΔURA3 by random integration method. After random integration of KmXYL1 and KmXYL2 genes, K. marxianus 17555-DF-33-14 strain was isolated for efficient ethanol production from xylose, through a directed evolutionary approach. In order to optimize fermentation conditions, agitation and medium pH conditions were varied using bioreactor. Xylose consumption rate, xylitol production rate and ethanol production rate were 1.50 g/L∙h, 0.73 g/L∙h and 0.02 g/L∙h, respectively at 600 rpm. However, ethanol production rate was 6 fold higher at 500 rpm (0.12 g/L∙h) than that from 600 rpm. In addition, when pH was controlled by 5.0, xylitol and ethanol concentration were increased, but cell growth was decreased. The optimum fermentation conditions were 500 rpm and pH 5.0 for ethanol production. Under the optimum conditions, xylose consumption rate, xylitol production rate and ethanol production rate were 1.05 g/L∙h, 0.43 g/L∙h and 0.13 g/L∙h, respectively.

키워드

저자

• Deok-Ho Kwon [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ]
• Jae-Bum Park [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ]
• Dae-Hyuk Kweon [ Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University ]
• Jin-Ho Seo [ Department of Agricultural Biotechnology, Seoul National University ]
• Suk-Jin Ha [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  강원대학교 산림과학연구소 학술대회

• 간기

  부정기

• 수록기간

  2017~2024

• 십진분류

  KDC 526 DDC 634