Earticle

초록

영어

Kluyveromyces marxianus 17555ΔURA3 strain was engineered by random integration of xylose metabolic pathway. Dual expression cassettes (KmXYL1-KmXYL2, KmXYL1-KmXYL3 and KmXYL2-KmXYL3) for xylose utilization were constructed using pJSKm316-GPD vector. Fermentation experiments were performed under different aeration conditions (micro aerobic or high aerobic) by engineered K. marxianus strains (17555-DC12, 17555-DC13 and 17555-DC23) at 30oC. The parental strain consumed 26.13 g/L xylose and produced 7.36 g/L xylitol and 2.05 g/L ethanol at micro aerobic conditions. In contrast, K. marxianus 17555-DC12 strain consumed 44.39 g/L xylose and produced 19.73 g/L xylitol and 5.72 g/L ethanol for 96 h. At high aerobic conditions, the parental strain consumed 31.01 g/L xylose and produced 9.62 g/L xylitol and 1.86 g/L ethanol. On the other hand, K. marxianus 17555-DC12 consumed 38.52 g/L xylose and produced 15.52 g/L xylitol and 5.10 g/L and ethanol for 72 h. Xylose consumption rate, xylitol production and ethanol production rates from K. marxianus 17555-DC12 were improved by 24%, 61% and 174%, respectively as compared to those from the parental strain at high aerobic conditions.

키워드

저자

• Deok-Ho Kwon [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ]
• Jae-Bum Park [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ]
• Dae-Hyuk Kweon [ Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University ]
• Jin-Ho Seo [ Department of Agricultural Biotechnology, Seoul National University ]
• Suk-Jin Ha [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  강원대학교 산림과학연구소 학술대회

• 간기

  부정기

• 수록기간

  2017~2024

• 십진분류

  KDC 526 DDC 634