2019 International Symposium of Institute of Forest Science (2019.09)바로가기
페이지
pp.89-89
저자
Zijiang Li, Rui Xu, Wei Liu, Jie Zhang, Lei Wu, Chuanling Si
언어
영어(ENG)
URL
https://www.earticle.net/Article/A450339
원문정보
초록
영어
The pine cones of Pinus pumila were extracted by ethanol. From the resulted residues, the polysaccharides of P. pumila cones were obtained by hot water extraction, spray drying and alcohol precipitation processes. The content of the polysaccharides was determined by phenol sulfuric acid method. The analysis of monosaccharide composition of the polysaccharide conducted by gas chromatography revealed that it was a heteropolysaccharide and consisted of L-arabinose, D-mannose, D-glucose and D-galactose in a molar ratio of 2.33:1.00:2.20:1.94, which indicated typical IR characteristics of polysaccharides. In addition, the antioxidant activity of polysaccharides was evaluated by measuring DPPH free radical and ABTS free radical scavenging capacity, as well as reducing capacity, and then the immunoregulatory activity of polysaccharides against RAW264.7 macrophages was evaluated by in vitro experiments. The results showed that pine cones polysaccharides of P. pumila exhibited significant scavenging effects on DPPH radicals, ABTS+ radicals and strong reducing power in a concentration-dependent manner. In addition, polysaccharides of P. pumila cones could stimulate RAW264.7 macrophages to produce a large amount of NO, but had no effect on cell proliferation. Overall, polysaccharides from Pinus pumila cones may have potential applications in the medical and food industries.
키워드
Pinus pumilaPolysaccharides of pine conesImmunoregulatory activityMonosaccharide compositionAntioxidant activity
저자
Zijiang Li [ Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science and Technology, Tianjin 300457, China; Shandong Institute of Commerce and Technology, Jinan 250103, China ]
Rui Xu [ Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science and Technology, Tianjin 300457, China ]
Wei Liu [ Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science and Technology, Tianjin 300457, China ]
Jie Zhang [ Shandong Institute of Commerce and Technology, Jinan 250103, China ]
Lei Wu [ Institute of Applied Chemistry, Jiangxi Academy of Sciences, Nanchang 330096, China ]
Corresponding Author
Chuanling Si [ Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science and Technology, Tianjin 300457, China ]
Corresponding author