Dahyeon Kim, Taeck Joong Jeon, Byeonggyu Park, Dong Yeop SHIN
언어
영어(ENG)
URL
https://www.earticle.net/Article/A448244
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4,200원
원문정보
초록
영어
Calcium ions play an important role in development and intracellular signaling . Dictyostelium discoideum has 14 genes encoding calcium -binding proteins (CBPs), but the function of most CBPs during development has not yet been studied . In this study, we investigated the specific functions of CBP7, one of 14 CBPs, in development using RNA interference cell lines of CBP 7, cell lines overexpressing CBP 7, cell lines with point mutations in the EF-hand domain , and cell lines expressing fragment proteins . was intended to reveal. CBP7 consists of 169 amino acids and contains 4 EF-hand domains. The CBP7-overexpressing cells showed complete loss of developmental process . These cells remained in the single-cell growth stage under development -inducing conditions , while wild-type cells formed aggregations within 6– 8 h of development and eventually formed fruiting bodies . The experiments using point-mutated CBP7 protein showed that all EFhand domains of CBP7 were important for CBP7 to function during developmental process . These results suggest that CBP 7 plays an important role in developmental processes across all EF-hand domains.
목차
Abstract 1. Introduction 2. Materials and Methods 2.1. Strains and cell culture 2.2. Fluorescence image acquisition 2.3. Development 3. Results 3.1. Identification of the gene encoding CBP7 3.2. Developmental defects induced by overexpression of CBP7 3.3. Development at various calcium concentrations 3.4. EF-hands in CBP7 are important in the process of development 4. Discussion 5. Conclusion References
키워드
CBP7DevelopmentDictyostelium Calcium
저자
Dahyeon Kim [ Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University ]
Taeck Joong Jeon [ Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University ]
Byeonggyu Park [ ALBIOTEK, 302, Galmachi-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do, Republic of Korea ]
Dong Yeop SHIN [ INVIVO Co. Ltd., 121, Daehak-ro, Nonsan 32992, Republic of Korea ]
Corresponding Author
조선대학교 기초과학연구원 [The Natural Science Research Institute of Chosun]
설립연도
2008
분야
자연과학>자연과학일반
소개
본 연구원은 기초과학을 진흥하기 위한 연구·교육 및 그 보급을 목적으로 한다. 이 목적을 달성하기 위하여 다음 각 호의 사업을 수행한다.
1. 기초과학 제 분야에 관한 조사와 연구
2. 기초과학에 관한 학술행사(학술대회, 학술세미나, 심포지엄, 초청강연회 등) 개최
3. 학문후속세대 및 일반인을 위한 기초과학 교육
4. 기관지『조선자연과학논문지』 발간
5. 『자연과학연구총서』, 『자연과학번역총서』 등 단행본 발간
6. 기타 본 연구원의 목적과 관련된 사업
간행물
간행물명
통합자연과학논문집(구 조선자연과학논문집) [Journal of Integrative Natural Science]