Earticle

초록

영어

Abstract : In this study the anti-oxidant, anti-inflammatory, and whitening effects of purple cabbage methanol extract (PM), purple cabbage hot-water extract (PW), brussels sprouts methanol extract (BM), brussels sprouts hot-water extract (BW), green cabbage methanol extract (GM), and green cabbage hot-water extract (GW) were evaluated. The anti-oxidant activities were assayed by three methods based on scavenging of DPPH, ABTS, and the total polyphenol content. PM and BM extracts showed the highest DPPH anion and ABTS cation radical scavenging activities. BM extract also exhibited the highest phenolic content (24.13 mg gallic acid/g). The anti-inflammatory activities on lipopolysaccharide (LPS)-induced RAW264.7 cells were also investigated. The BM significantly suppressed NO production, cytokine release (TNF-α, IL-6, and PGE2), and inflammatory gene (iNOS and COX-2) expression. Furthermore, the BM extract inhibited in vitro mushroom tyrosinase activity and α-MSH-induced extracellular melanin content in B16F10 cells. Based on these results, extracts of brussels sprouts could be a candidate for anti-oxidant, anti-inflammatory and whitening agents.

중국어

摘要:本研究共采集了6类样品:红甘蓝甲醇提取物(PM), 红甘蓝热水提取物(PW), 球子甘蓝 甲醇提取物(BM), 球子甘蓝热水提取物(BW)􀕵甘蓝甲醇提取物(GM)和卷心菜热水提取物 (GW), 旨在于评价其抗氧化􀕵抗炎和美白功效, 根据DPPH, ABTS和总多酚含量对抗氧化活 性进行了比较分析, 研究结果表明, PM和BM提取物对阴离子DPPH自由基和阳离子ABTS的 自由基清除能力较高, 且BM提取物的总多酚含量最好, 为24.13 mg gallic acid/g, 针对抗 炎活性的研究则通过 LPS 诱导 RAW264.7 细胞株炎症来实施, 结果表明, BM 提取物对NO 生成, TNF-α, IL-6 和 PGE2 分泌以及与 iNO 和 COX-2炎症相关基因的生成水平呈现出显 著的抑制作用, 此外􀕵BM 提取物在in vitro mushroom tyrosinase的激活, B16F10 细胞株 中抑制基于 α-MSH 的黑色素生成, 综上, 可以推断球子甘蓝提取物作为抗氧化剂, 抗炎和 美白候选物质具有巨大潜力.

목차

Abstract
I. 서론
II. 이론적 배경
1. 활성산소와 피부 노화
2. 피부 항노화 기능: 항산화제
3. 피부 항노화 기능: 항염제
4. 피부 항노화 기능: 미백제
III. 연구방법
1. 소재 준비 및 추출
2. DPPH radical scavenging assay
3. ABTS radical scavenging assay
4. Total polyphenol content assay
5. WST-8 cell viability assay
6. Griess reagent assay
7. Western blot assay
8. Cytokine Enzyme Linked Immunosorbent Assay(ELISA)
9. In vitro mushroom tyrosinase inhibition assay
10. Extracellular melanin content assay
11. 통계
III. 결과 및 고찰
1. 방울양배추 추출물의 수율
2. 방울양배추 추출물의 항산화 효능
3. 세포 생존율
4. 방울양배추 추출물의 LPS 유도에 의한 항염 효능
5. 티로시나아제 저해능
IV. 결론
참고문헌
中文摘要

키워드

저자

• 김준희 [ Jun Hi Kim | 서경대학교 일반대학원 미용예술학과 학생 ]
• 이지안 [ Ji-An Lee | 서경대학교 미용예술대학 뷰티테라피 & 메이크업학과 교수 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  국제보건미용학회지 [Journal of Health and Beauty]

• 간기

  계간

• pISSN

  2005-369X

• eISSN

  2765-1347

• 수록기간

  2007~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 593 DDC 646