Earticle

초록

영어

Purpose: We wished to determine the optimal method for the extraction of polysaccharides with antioxidant activities from Polygonatum odoratum . Methods: Single-factor and orthogonal tests were applied to identify the best parameters for water extraction and fermentation. The solid-liquid ratio, temperature, and duration of water extraction were determined according to the total polysaccharide content. The fermentation temperature, agitation speed, inoculation amount, and fermentation time were investigated according to the optical density of Saccharomyces cerevisiae. The molecular weight distributions of water- and fermentation-extracted polysaccharides were determined by high performance gel permeation chromatography. The antioxidant activities of the polysaccharides were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) ABTS radical scavenging capacities, and ferric reducing antioxidant power (FRAP). Results: The optimum water extraction parameters were 80°C for 2.5 h with a 1:60 solid-liquid ratio. For fermentation, an 8% extraction volume was inoculated with S. cerevisiae . Optimal fermentation occurred at 28°C for 28 h, with an agitation speed of 180 r/min. The molecular weights of water- and fermentation-extracted polysaccharides were 2.067×104 and 9.475×103 Da, respectively. The DPPH, ABTS, and FRAP results demonstrated an obvious increase in antioxidant activity after fermentation. Conclusion: The fermentation extraction of P. odoratum may provide valuable materials for future cosmetic use.

한국어

목적: 본 연구는 Polygonatum odoratum으로부터 항산화 활성을 갖는 다당류를 추출하기 위한 최적의 방법을 결정하고자 한다. 방 법: 최적의 물 추출 발효 공정 변수를 결정하기 위해 단일 요인 및 직교 실험을 진행하였다. 총 다당류 함량에 따라 물질 대 액체 비 율, 온도, 물담근 시간을 결정하였다. Saccharomyces cerevisiae의 흡광도를 지표로 하여 발효온도, 교반속도, 접종량, 발효시간을 조사하였다. 물 추출 다당류와 발효 추출 다당류의 분자량 분포를 결정하기 위해 고성능 겔 투과 크로마토그래피를 사용하였다. 다 당류의 항산화 활성은 DPPH와 ABTS 라디칼 소거능, FRAP (철환원 항산화력)을 이용하여 평가하였다. 결과: 최적의 물 추출 매 개변수는 고액비1:60, 온도 80℃, 추출시간 2.5 h이다. 발효를 위해 추출 부피의 8%에 S. cerevisiae를 접종하였다. 최적의 발효조 건은 28℃에서 28 h, 교반속도는 180 r/min이다. 물추출 다당류와 발효추출 다당류의 분자량은 각각 2.067×104 Da, 9.475×103 Da이었다. DPPH, ABTS, FRAP 항산화 활성이 발효 후 크게 향상되었다. 결론: Polygonatum odoratum의 발효 및 추출 과정은 Polygonatum odoratum의 화장품산업에 응용될 수 있는 원료를 제공할 수 있다고 사료된다

중국어

目的: 研究从玉竹中提取抗氧化多糖的最佳工艺。方法: 采用单因素试验和正交试验确定最佳水提发酵工艺参 数。根据多糖总含量确定料液比、温度和水浸时间。以酿酒酵母的光密度为指标，考察发酵温度、搅拌速度、 接种量和发酵时间。采用高效凝胶渗透色谱法测定了水提多糖和发酵提多糖的分子量分布。以DPPH和ABTS 自由基清除能力和铁还原抗氧化能力(FRAP)评价多糖的抗氧化活性。结果: 最佳水提工艺参数为80℃，料液比 1:60，提取2.5 h。发酵时，以8%的萃取量接种酿酒酵母。最佳发酵条件为28℃发酵28 h，搅拌速度为180 r/ min。水提多糖和发酵多糖的分子量分别为2.067×104和9.475×103 Da。发酵后DPPH、ABTS和FRAP的抗氧化 活性明显提高。结论: 玉竹发酵提取工艺可为玉竹的美容应用提供有价值的原料。

목차

Abstract
Introduction
Methods
1. Chemicals and reagents
2. Extraction methodds
3. Total polysaccharide content
4. Single-factor tests for optimal water extraction
5. Orthogonal test for water extraction
6. Fermentation process
7. Single-factor tests for optimal fermentation
8. Orthogonal test for fermentation optimization
9. Molecular weight distribution assay
10. Determination of DPPH radical scavenging activity
11. Scavenging of ABTS radicals
12. Total antioxidant capacity assay
13. Statistical analyses
Results
1. Optimization of extraction conditions for water-soluble polysaccharides
2. Optimization of fermentation conditions
3. Molecular weight distribution
4. DPPH radical scavenging activity
5. ABTS radical scavenging activity
6. Total antioxidant capacity
Discussion
Conclusion
Acknowledgements
Author's contribution
Author details
References
국문초록
中文摘要

키워드

Polygonatum odoratum 당류 발효 분자량 항산화 Polygonatum odoratum Polysaccharides Fermentation Molecular weight Antioxidant activity 玉竹，多糖，发酵，分子量，抗氧化活性

저자

• Junchen Liu [ 류준신 | Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing, China ]
• Zaijun Geng [ 경재군 | Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing, China ]
• Hui Huang [ 황혜 | Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing, China ]
• Miaomiao Guo [ 곽묘묘 | Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing, China ]
• Li Li [ 리려 | Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing, China ] Corresponding Author

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