Earticle

초록

영어

This study aims to assess the anti-oxidant, anti-inflammatory, and anti-microbial effects of Suaeda australis, which is known as halophytes. Methanol extracts of S. australis were fractioned according to the polarity with n-hexane, chloroform, ethyl acetate, n-butanol, and H2O. The anti-oxide activity of the fractions was evaluated using DPPH radical scavenging activity and total polyphenol content. All solvent fractions showed potent anti-oxidative effects, as assessed using radical scavenging activity, with IC50 values ranging from 109.782 to 754.691 ㎍/mL. In agreement with this pattern, the total phenolic contents were widely distributed in solvent fractions, and ranged from 10.746 to 50.672 mg/g GAE of dry weight. In the evaluation of anti-inflammatory effects, LPS-activated RAW264.7 macrophages suppressed the expression of iNOS and COX-2, as well as the production of TNF-α and IL-6. Skin barrier protective effects were investigated in HaCaT cells measuring the mRNA expression of skin barrier integrity and hydration genes, namely, HBD (human β-defensin), LL-37(cathelicidin), and HAS (hyaluronic acid synthase). The ethyl acetate fraction showed the strongest skin barrier integrity. Based on the current results, S. australis was suggested to posses natural anti-oxidant and anti-inflammatory agents, and skin protective materials, indicating that it could be a valuable source of multi-functional cosmetic ingredients.

중국어

本研究旨在于调查盐生植物南方碱蓬地上部分提取物的抗氧化, 抗炎, 抗微生物肽及皮肤保 湿功效. 本文以南方碱蓬甲醇提取物为对象, 使用不同极性的有机溶剂, n-hexane, chloroform, ethyl acetate, n-butanol及水制造了分划物. 通过DPPH径向清除活性和总多 酚含量的测定, 对其抗氧化功效分析的结果显示, 分划物的DPPH IC50值在109.782至754.691 微克/mL浓度范围内, 与此相同, 总多酚含量结果为10.746至50.672 mggAE/g, 所有分划物 均显示出抗氧化功效. 通过确认LPS诱导炎症反应的RAW264.7巨噬细胞中的抗炎功效的结果 显示, NO生成和TNF-α, IL-6等血清素, iNOS和COX-2的产生均受到了阻碍. 最后, 将南方 碱蓬的皮肤抗微生物肽和保湿基因, HBD (humanβ-对defensin, LL-37(cathelicidin), and HAS(hyaluronic acid synthase) mRNA的生成物, 在HaCaT角质细胞中进行了调查, 结果表明, 乙酰乙酸分泌物具有最佳的细胞屏障保护效果. 综合上述实验, 南方碱蓬作 为天然抗氧化剂, 抗炎剂和护肤成分功效的多功能化妆品原料, 其价值较高.

목차

Abstract
I. 서론
II. 이론적 배경
1. 피부 기능
2. 항산화제와 피부장벽
3. 항염제와 피부장벽
4. 항미생물 펩타이드
III. 연구방법
1. 추출 및 수율
2. 메탄올추출물과 분획물의 DPPH 전자공여능 측정
3. 분획물의 ABTS 양이온 라디컬 소거능 측정
4. 분획물 내 폴리페놀 함량 측정
5. 분획물에 의한 세포 독성 측정
6. Nitric oxide (NO) 생성량 측정
7. iNOS 및 COX-2의 단백질 발현 변화 측정
8. 염증성 싸이토카인 변화량 측정
9. 항미생물 펩타이드 및 수분 유전자 mRNA 발현 수준 측정
10. 통계
IV. 결과 및 고찰
1. 방석나물 메탄올 추출물의 DPPH 라디컬 소거 활성
2. 방석나물 지상부 메탄올추출물의 DPPH 라디컬 소거 활성
3. 메탄올추출물과 분획물의 항산화 활성
4. 세포 생존율
5. NO 생성 억제 효과
6. 염증성 싸이토카인 분비 억제 효능
7. 염증 관련 iNOS 및 COX-2 효소 단백질 발현 저해효과
8. 항미생물 펩타이드 및 피부 보습 유전자 mRNA 발현 증가
IV. 결론
참고문헌
中文摘要

키워드

저자

• 박지효 [ Ji Hyo Park | 서경대학교 일반대학원 미용예술학과, 학생 ]
• 이지안 [ Ji-An Lee | 서경대학교 미용예술대학 뷰티테라피 & 메이크업학과, 교수 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  국제보건미용학회지 [Journal of Health and Beauty]

• 간기

  계간

• pISSN

  2005-369X

• eISSN

  2765-1347

• 수록기간

  2007~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 593 DDC 646