Earticle

초록

영어

Purpose: This study aims to verify the biological activities of leaf and flower extracts of Osmanthus fragrans var. aurantiacus (OF) and Osmanthus asiaticus Lour. var. fragrans (OA), which can be used in the cosmetics industry. Methods: Ethanol extraction of the flowers and leaves of OF and OA was performed to determine the total polyphenol and flavonoid content, the scavenging activities of DPPH and ABTS radicals, the inhibitory effects on elastase and tyrosinase, and antibacterial effects. Results: The total polyphenol content of leaf and flower extracts was determined to be 135.64 μg/mL and 80.06 μg/mL, respectively, for OF and 104.11 μg/mL and 71.64 μg/mL, respectively, for OA. The total flavonoid content of leaf and flower extracts was found to be 123.48 μg/mL and 86.20 μg/mL, respectively, for OF and 104.07 μg/mL and 78.13 μg/mL, respectively, for OA. At a concentration of 500 μg/ mL, the DPPH scavenging activity of leaf and flower extracts was found to be 84.49% and 72.08%, respectively, for OF and 77.12% and 68.75%, respectively, for OA. The ABTS radical scavenging activity of leaf and flower extracts was determined to be 90.36% and 83.15%, respectively, for OF and 88.14% and 80.90%, respectively, for OA. The inhibitory effect of leaf and flower extracts on elastase was confirmed to be 58.31% and 46.14%, respectively, for OF and 51.22% and 39.26%, respectively, for OA. The inhibitory effects of leaf and flower extracts on tyrosinase were found to be 57.24% and 48.12%, respectively, for OF and 52.15% and 41.12%, respectively, for OA. Upon evaluation, the leaf and flower extracts of OF and OA exhibited antibacterial effects on Propionibacterium acnes (P. acnes ) and Malassezia furfur (M. furfur ). Conclusion: Among the ethanol extracts of the leaves and flowers of both species, the leaf extract of OF was confirmed to have not only an antioxidant effect but also a high inhibitory effect on elastase and tyrosinase, along with antibacterial effects. Therefore, it is expected to have high potential as a cosmetic material.

한국어

목적: 본 연구에서는 금목서, 은목서 잎과 꽃 추출물에 대한 생리활성 검증을 통해 향장 산업계에서 활용될 수 있는 기초 데이터를 제공하고자 한다. 방법: 금목서, 은목서 잎과 꽃을 에탄올로 추출하여 총 폴리페놀과 플라보노이드 함량, DPPH, ABTS 라디컬 소거 능, elastase, tyrosinase 저해효과 그리고 항균효과를 측정하였다. 결과: 총 폴리페놀 함량은 금목서 잎과 꽃 추출물은 135.64 μg/ mL, 80.06 μg/mL으로 확인되었으며, 은목서 잎과 꽃 추출물은 104.11μ g/mL, 71.64 μg/mL 함량이 확인되었다. 총 플라보노이드 함량은 금목서 잎과 꽃 추출물은 123.48 μg/mL, 86.20 μg/mL으로 확인되었으며, 은목서 잎과 꽃 추출물은 104.07 μg/mL, 78.13 μg/mL함량이 확인되었다. 500 μg/mL 농도에서 DPPH 라디컬 소거능은 금목서 잎과 꽃 추출물은 84.49%, 72.08%, 은목서 잎과 꽃 추출물은 77.12%, 68.75%의 소거능이 확인되었으며, ABTS 라디컬 소거능은 금목서 잎과 꽃 추출물은 90.36%, 83.15%, 은목 서 잎과 꽃 추출물은 88.14%, 80.90% 확인되었다. Elastase 저해효과는 금목서 잎과 꽃 추출물은 58.31%, 46.14%, 은목서 잎과 꽃 추출물은 51.22%, 39.26% 확인되었다. Tyrosinase 저해효과는 금목서 잎과 꽃 추출물은 57.24%, 48.12%, 은목서 잎과 꽃 추출물 은 52.15%, 41.12% 확인되었다. P. acnes, M. furfur의 항균효과를 측정한 결과 금목서, 은목서 잎과 꽃 추출물은 항균효과가 확 인되었다. 결론: 따라서 금목서, 은목서 잎과 꽃 에탄올 추출물 중, 금목서 잎 추출물은 항산화 효과를 가질 뿐만 아니라, elastase, tyrosinase 저해효과도 높게 확인 되었으며, 항균효과에도 뛰어나 향장소재로서의 적용 가능성 또한 높을 것으로 사료된다

중국어

目的: 本研究旨在通过验证金桂以及银桂的叶和花提取物的生理活性，为化妆品行业提供可使用的基础数据。方 法: 通过提取金桂以及银桂的叶和花，分别测定其总多酚和黄酮含量、DPPH、ABTS自由基清除活性、弹性蛋白 酶、酪氨酸酶抑制作用和抗菌作用。结果: 发现金桂叶和花提取物中的总多酚含量分别为135.64 μg/mL和80.06 μg/mL，银桂的叶和花提取物中的总多酚含量分别为104.11 μg/mL和71.64 μg/mL。金桂叶和花提取物的总黄 酮含量分别为123.48 μg/mL和86.20 μg/mL，银桂的叶和花提取物的总黄酮含量分别为104.07 μg/mL和78.13 μg/mL。在浓度为500 μg/mL时，金桂的叶和花提取物的DPPH自由基清除活性分别为84.49% 和72.08%，银 桂的叶和花提取物的DPPH自由基清除活性分别为77.12%和68.75%；金桂的叶和花提取物的ABTS自由基清除 活性为90.36%和83.15%，而银桂的叶和花提取物的ABTS自由基清除活性为88.14%，80.90%；金桂的叶和花 提取物的弹性蛋白酶抑制作用分别为58.31%和46.14%，而银桂的叶和花提取物的弹性蛋白酶抑制作用分别为 51.22%和39.26%；金桂的叶和花提取物的酪氨酸酶抑制效果分别为57.24%和48.12%；银桂的叶和花提取物的 酪氨酸酶抑制效果分别为52.15%和41.12%。作为测量P. acnes 和M. furfur 的抗菌效果的结果，金桂以及银桂的 叶和花提取物都具有抗菌效果。结论: 因此，金桂以及银桂的叶和花提取物乙醇提取物中，金桂的叶提取物不仅 具有抗氧化作用，而且对弹性蛋白酶和酪氨酸酶具有较高的抑制作用。

목차

Abstract
Introduction
Methods
1. 실험재료 및 추출방법
2. 총 폴리페놀 함량 측정
3. 총 플라보노이드 함량 측정
4. DPPH 라디컬 소거능 측정
5. ABTS 라디컬 소거능 측정
6. Elastase 저해효과 측정
7. Tyrosinase 저해효과 측정
8. 항균효과 측정
9. 통계
Results
1. 총 폴리페놀 및 총 플라보노이드 함량
2. DPPH 라디컬 소거능
3. ABTS 라디컬 소거능
4. Elastase 저해효과
5. Tyrosinase 저해효과
6. 항균효과
Conclusion
References
국문초록
中文摘要

키워드

생리활성 화장품 엘라스타제 티로시나제 항균 Biological activity Cosmetic Elastase Tyrosinase Antibacterial 生理活性，化妆品，弹性蛋白酶，酪氨酸酶，抗菌

저자

• Oh Sun Yoon [ 윤오선 | Department of Cosmetology, Cheong Am College, Suncheon-si, Jeollanam-do, Korea ] Corresponding Author

참고문헌

발행기관

간행물