Earticle

초록

영어

Purpose: This study aimed to investigate the use of purified Kaki-tannin obtained from a Korean natural product, Diospyros kaki Thumb. In order to confirm the potential application of kaki-tannin to cosmetics, the efficacy was evaluated by kaki-tannin that molecular weight decreased by gamma irradiation. Methods: Kaki-tannin polymer was extracted by hydrothermal extraction from persimmon. The molecular weight of Kaki-tannin polymer reduced by gamma-ray irradiation, and then separated and purified. Further, the molecular weight of this polymer was reduced by gamma-ray irradiation; subsequently, it was separated and purified. Radical scavenging activities of this reduced molecular weight Kaki-tannin were examined by the 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt radical cation, and hydroxyl radical scavenging assays. Moreover, the inhibitory effects of Kaki-tannin on tyrosinase and melanin synthesis in B16F10 melanoma cells were measured in vitro. Furthermore, the expression of tyrosinase, microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP)-1, and TRP-2 during melanogenesis was confirmed by western blotting. Results: Irradiated Kaki-tannin exhibited an antioxidant effect as well as suppressed tyrosinase activity and melanogenesis. The results of this study confirmed that the expression of tyrosinase, MITF, and TRP-2 decreased as the treatment of irradiated Kaki-tannin increased. Conclusion: In the present study, to incorporate high molecular weight Kaki-tannin in cosmetics, it was first irradiated with gamma rays in order to reduce its weight. This study confirmed that Kaki-tannin irradiated by gamma-ray has antioxidant and whitening effects as well as the potential to be used as a cosmetic material.

한국어

목적: 화장품의 기초 소재 개발방안 중 하나로, 국내 천연물인 청도 감을 이용하여 감 탄닌을 추출하였다. 30,000 Da의 큰 분자량 을 가지는 감탄닌을 화장품에 효능 성분으로 이용 가능하도록 방사선 조사를 통하여 저분자화 하여 그 효능을 평가하였다. 방법: 감을 시료로 하여 열수추출을 통하여 고분자 감탄닌을 추출하였으며 고분자 감탄닌에 Gamma ray를 조사하여 저분자화 하고 분 리 정제 하였다. 방사선이 조사된 감탄닌에서 DPPH, ABTS+ 및 Hydroxyl 라디칼 소거능을 측정하였으며, in vitro상의 tyrosinase 활성 저해 효과와 B16F10 melanoma cell에서의 멜라닌 합성 억제 효과를 관찰하였다. 또한 그 때의 멜라닌 합성 관련 단백질 발 현량을 western blotting을 통하여 확인하였다. 결과: In vitro 시험 결과, 방사선이 조사된 감탄닌은 항산화 효과를 나타내었으 며, tyrosinase의 활성과 melanogenesis가 억제 됨을 확인하였다. 또한 방사선이 조사된 감탄난의 시료 농도가 증가함에 따라 Tyrosinase, MITF, TRP-2 발현양이 감소함을 확인하였다. 결론: 본 연구를 통하여 고분자 감탄닌에 방사선을 조사하여 저분자 감 탄닌을 만들고 제조된 저분자 감탄닌이 항산화, 미백 효능을 나타내어 화장품 소재로써 이용될 잠재력이 있음을 확인였하다.

중국어

目的: 本研究旨在研究从韩国天然产品柿蒂（Diospyros kaki Thumb）中提取的纯化柿子单宁在化妆品中的潜 在应用。此外，通过γ射线照射降低其分子量来评估柿子单宁的功效。方法: 首先通过热水提取法提取高分子 柿子单宁聚合物。此外，通过γ射线照射其聚合物的分子量降低，随后，进行分离和纯化。利用对γ射线照射的 Kaki-tannin进行 DPPH、ABTS+ 和羟基自由基清除活性测定。此外，在体外测量了柿子单宁对B16F10黑色素 瘤细胞中酪氨酸酶和黑色素合成的抑制作用。此外，蛋白质印迹法证实了黑素生成过程中酪氨酸酶、小眼相关 转录因子 (MITF)、酪氨酸酶相关蛋白 (TRP)-1和TRP-2的表达。结果: 经辐照的柿单宁表现出抗氧化作用以及抑 制酪氨酸酶活性和黑色素生成。这项研究的结果证实，酪氨酸酶、MITF 和 TRP-2的表达随着辐照柿单宁处理的 增加而降低。结论: 在本研究中，为了在化妆品中加入高分子柿子单宁，首先用γ射线照射以减少其分子量。经 研究发现，照射的柿子单宁具有抗氧化和美白作用，并有潜力用作化妆品原料。

목차

Abstract
Introduction
Methods
1. 시료 제조
2. 세포주 배양
3. DPPH 라디칼 소거능 측정
4. ABTS+ 라디칼 소거능 측정
5. Hydroxyl 라디칼 소거활성 측정
6. Cell viability 측정
7. Cellular tyrosinase 활성 측정
8. Melanin 생합성 저해 측정 및 관련 단백질 발현에 미치는 영향 분석
9. 통계분석
Results and Discussion
1. Kaki-tannin oligomer의 분석결과
2. 감마선 조사된 감탄닌의 DPPH 라디칼 소거능
3. 감마선 조사된 감탄닌의 ABTS+ 라디칼소거활성 확인
4. 감마선 조사된 감탄닌의 Hydrxoyl 라디칼소거활성 확인
5. 감마선이 조사된 감탄닌의 B16F10 cell의 세포 독성평가 확인
6. 감마선 조사된 감탄닌의 tyrosinase 활성 억제 확인
7. 감마선 조사된 감탄닌의 melanin 합성 억제 확인
8. 감마선 조사된 감탄닌의 tyrosinase, MITF, TRP-1, TRP-2 단백질 발현 억제능 확인
Conclusion
References
국문초록
中文摘要

키워드

저자

• Kyeong Seok So [ 소경석 | CIR R&D Center, Cosmecca Korea Ltd., Research Institute for Molecular- Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul, Korea ]
• Jin-Hyun Kim [ 김진현 | CIR R&D Center, Cosmecca Korea Ltd., Seongnam-si, Gyeonggi-do, Korea ]
• Yeo Jin Lim [ 임여진 | CIR R&D Center, Cosmecca Korea Ltd., Research Institute for Molecular- Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul, Korea ]
• Ju Tae Jeong [ 정주태 | CIR R&D Center, Cosmecca Korea Ltd., Seongnam-si, Gyeonggi-do, Korea ]
• Jae Yong Seo [ 서재용 | CIR R&D Center, Cosmecca Korea Ltd., Research Institute for Molecular- Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul, Korea ]
• Young Kwon Cha [ 차영권 | CIR R&D Center, Cosmecca Korea Ltd., Seongnam-si, Gyeonggi-do, Korea ]
• Hyun Dae Cho [ 조현대 | CIR R&D Center, Cosmecca Korea Ltd., Seongnam-si, Gyeonggi-do, Korea ]
• Hang Eui Cho [ 조항의 | CIR R&D Center, Cosmecca Korea Ltd., Seongnam-si, Gyeonggi-do, Korea ] Corresponding Author
• Seunghee Bae [ 배승희 | Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  아시안뷰티화장품학술지 [Asian Journal of Beauty and Cosmetology]

• 간기

  계간

• pISSN

  2466-2046

• eISSN

  2466-2054

• 수록기간

  2016~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 593 DDC 646