Earticle

초록

영어

Purpose: In this study, the effect on skin elasticity of a cream containing threedimensional human adipose tissue derived mesenchymal stem cell conditioned medium (3D ADMSC-CM) was examined. Methods: The clinical efficacy of the cream was evaluated by examining the skin for skin lifting, elasticity, and resilience after examining the cell proliferative capacity, expression levels of epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) in 3D ADMSC-CM, and collagen expression in skin cells due to the 3D ADMSC-CM cream. Results: Skin cell viability was well maintained, with no cytotoxicity from the 3D ADMSC-CM. The amount of EGF and VEGF contained in the 3D ADMSC-CM was 2.692 pg/mL and 27.89 pg/mL, respectively. In addition, skin cells treated with the 3D ADMSC-CM cream experienced a collagen expression increase of 183%. In clinical evaluation of the 3D ADMSC-CM cream, skin lifting and skin elasticity improvement showed significant effects at both two and four weeks. After the 3D ADMSC-CM cream was used, the improved skin elasticity and resilience was analyzed by variables, and an improvement was observed in both after two and four weeks of use. Conclusion: A cream containing 3D ADMSC-CM derived from a 3D culture environment, similar to that of an in vivo environment, is highly effective in enhancing skin elasticity, and 3D ADMSC-CM is expected to lead the field of cosmeceuticals.

한국어

목적: 본 연구에서는 3차원 지방조직유래 중간엽줄기세포 조건화 배지(three-dimensional human adipose tissue derived mesenchymal stem cell conditioned medium, 3D ADMSC-CM)를 함유한 크림이 피부탄력에 미치는 효능에 관한 연구를 진행하 고자 한다. 방법: 3D ADMSC-CM의 세포 증식능력, EGF와 VEGF의 발현량을 확인한다. 3D ADMSC-CM이 함유된 크림의 콜라 겐(collagen1) 발현량을 검증하며, 리프팅, 피부탄력, 피부탄성복원력 등 임상적 효능 평가를 진행한다. 결과: 인간 진피유래 섬유 아 세포의 형태적 변화가 관찰되지 않았으며, 농도 의존적으로 세포의 생존율이 증가하는 것으로 나타났다. EGF의 분비량은 2.692 pg/mL, VEGF는 27.89 pg/mL을 분비하였다. 3D ADMSC-CM 크림의 콜라겐(collagen 1)이 발현량은 183%로 증가하는 것을 확 인 할 수 있었다. 3D ADMSC-CM 크림 사용에 의한 임상적 효능 평가에서 리프팅 효과를 측정한 결과 적용 2주 후와 4주 후에 유 의미한 효과를 보였다. 피부탄력 및 피부탄성복원력을 변수 별 측정값을 분석한 결과 적용 2주 후와 적용 4주 후에 모두 개선 효과 가 있는 것으로 나타났다. 결론: 본 연구에서 인체 구조와 비슷한 환경에서 배양된 3D ADMSC-CM이 함유된 크림을 통해 피부탄 력 증진에 매우 효과적이며, 3D ADMSC-CM은 코스메슈티컬 시장을 선도할 것으로 기대된다

중국어

目的: 在本研究中，研究了含有三维人体脂肪组织来源的间充质干细胞条件培养基(three-dimensional human adipose tissue derived mesenchymal stem cell conditioned medium, 3D ADMSC-CM)的霜对皮肤弹性的影 响。方法: 检查3D ADMSC-CM的细胞增殖能力和EGF、VEGF的表达水平。验证含有3D ADMSC-CM的霜的胶原 蛋白(collagen1)表达水平，并评估提升、皮肤弹性和皮肤弹性恢复的临床疗效。结果: 皮肤细胞活力保持良好， 3D ADMSC-CM没有细胞毒性。3D ADMSC-CM中 EGF和VEGF的含量分别为2.692 pg/mL和27.89 pg/mL。此 外，用3D ADMSC-CM霜处理的皮肤细胞的胶原蛋白表达增加了183%。在3D ADMSC-CM霜的临床评估中，皮 肤提升和皮肤弹性改善在两周和四周均显示出显着效果。使用3D ADMSC-CM霜后，通过变量分析改善的皮肤弹 性和皮肤弹性恢复的测量值，发现使用两周和四周后均观察到改善。结论: 含有类似于体内环境的3D培养环境中 的3D ADMSC-CM的乳霜在增强皮肤弹性方面非常有效，3D ADMSC-CM有望引领药妆领域。

목차

Abstract
Introduction
Methods
Ⅰ. 세포 실험(in vitro )
Ⅱ. 인체적용시험
Ⅲ. 통계분석
Results and Discussion
1. 세포 증식능력 평가
2. EGF, VEGF growth factor
3. Collagen 단백질 발현량 측정
4. 인체적용시험
Conclusion
References
국문초록
中文摘要

키워드

3차원 줄기세포배양액 탄력 콜라겐 EGF 임상적 효능 Three-dimensional human adipose tissue derived mesenchymal stem cell conditioned medium Elasticity Collagen Epidermal growth factor Clinical efficacy 3D人体脂肪组织来源间充质干细胞条件培养基，弹性，胶原蛋白，表皮生长因子，临床疗效

저자

• Kyung Hye Kim [ 김경혜 | Department of Cosmetics Engineering, Konkuk University, Cosemeceutical Team, DongKoo Bio & Pharma, Seoul, Korea ]
• Sunray Lee [ 이순례 | Stem Cell Niche Division, CEFO Research Center, Seoul, Korea ]
• Seunghee Bae [ 배승희 | Department of Cosmetics Engineering, Konkuk University, Seoul, Korea ]
• Sungkwan An [ 안성관 | Department of Cosmetics Engineering, Konkuk University, Seoul, Korea ] Corresponding author

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