Earticle

초록

영어

Purpose: To investigate the cosmetic effects of a chambirum (Amaranthus mangostamus ) extract on the skincare by measuring the extract’s antioxidant, antiwrinkle, and whitening. Methods: To confirm the antioxidant effect of chambirum, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and superoxide dismutase (SOD)-like activity was determined. To confirm whitening effect of chambirum, tyrosinase inhibitory activity and melanin content were investigated. To confirm anti-wrinkle effect of chambirum, the elastase inhibition effect and type I procollagen production was assayed. B16F10 mouse melanoma cells and CCD986sk human dermal fibroblast cells were used to assay cell viability. Results: The DPPH radical scavenging activity of Amaranthus mangostanus ethanol extracts (AME) and Amaranthus mangostanus water extracts (AMW) was 49% and 56%, respectively, at 500 μg/mL. The SOD-like activity of AME and AMW was 67% and 57%, respectively, compared with that of the standard sample L-ascorbic acid at 38% at 1 mM. AME showed 25.9% (L-tyrosine) and 17.8% (L-DOPA), and AMW showed 23.4% (L-tyrosine) and 18.5% (L-DOPA) of tyrosinase inhibition assay. In addition, the intracellular melanin production in B16F10 mouse melanoma cells was decreased by AME and AMW by 25.5% and 24.0%, respectively. Additionally, the elastase inhibition assay results demonstrated inhibition by AME (19.7%) and AMW (17.2%) at 500 μg/mL and the amount of type I procollagen was shown to be insignificant. Furthermore, B16F10 mouse melanoma cells were 76% and 86% showed a viability 76% and 86% when treated with 100 μg/mL AME and AMW, respectively. CCD- 986sk fibroblast cells showed a viability 94.5% and 83.6% when we treated with 100 μg/mL AME and AMW, respectively. Conclusion: These results suggest that the chambirum (Amaranthus mangostamus ) extract has excellent antioxidants, antiwrinkle, and whitening effects. Therefore, it may have a significant industrial value as a functional material for cosmetic or anti-aging related bioproducts.

한국어

목적: 나물로만 사용되는 참비름(Amaranthus mangostanus) 추출물을 대상으로 화장품 소재적 특성을 조사하기 위하여 항산화 효과, 미백 활성 및 주름개선 효능을 진행하였다. 방법: 항산화 활성으로는 1,1-diphenyl-2-picrylhydrazyl (DPPH)와 superoxide dismutase (SOD)-like activity, 미백활성으로는 tyrosinase 저해 활성과 melanin 함량을 조사하였고, 주름개선 효능으로는 elastase 저해 활성과 type 1 pro-collagen을 측정하였다. B16F10 mouse melanoma cells과 CCD986sk human dermal fibroblast cells을 대 상으로 세포 생존력을 측정하였다. 결과: DPPH 방법으로 조사한 항산화 활성은 시료의 농도 500 μg/mL의 농도에서 Amaranthus mangostanus ethanol extracts (AME) (49%), Amaranthus mangostanus water extracts (AMW) (56%)의 radical 소거 활성을 나타 내었고, SOD-like activity를 측정한 결과, 시료의 농도는 동일하게 500μ g/mL에서 AME (67%), AMW (57%)의 활성을 나타내었다 . 미백활성으로는 L-tyrosine을 기질로 사용한 tyrosinase 저해 활성은 AME (25.9%), AMW (23.4%)로 나타내었고 L-DOPA의 경 우 AME (17.8%), AMW (18.5%)의 높은 미백효과를 확인 할 수 있었다. B16F10 mouse melanoma cell line을 대상으로 melanin양 을 측정한 결과, AME (25.5%), AMW (24.0%)의 melanin 감소율을 나타내었다. 주름개선 효능으로는, 시료의 농도, 500 μg/mL에 서 elastase 저해 활성은 AME (19.7%), AMW (17.2%)로 조사되었고, type I opcrollagen의 collagen 양은 미미한 것으로 나타내었다 . B16F10 mouse melanoma cells에 대한 세포 독성은 시료의 농도 100 μg/mL에서 AME (76%), AMW (86%)의 세포 생존율을 나타 내었고, CCD-986sk fibroblast cells에 대한 세포독성 결과는 시료의 농도 100 μg/mL에서 AME (94.5%), AMW (83.6%)의 생존율 을 확인할 수 있었다. 결론: 참비름 추출물은 항산화 및 미백 활성에 탁월한 효능을 보여주었고, 천연물 유래의 화장품 소재로서의 가능성과 항노화 산업의 잠재적인 소재로서의 가치를 확인할 수 있었다.

중국어

目的: 通过测量苋菜(Amaranthus mangostamus )提取物的抗氧化、抗皱和美白作用，研究其提取物对护肤品 的美容效果。方法: 为确定苋菜的抗氧化活性，测定其1,1-diphenyl-2-picrylhydrazyl (DPPH)和superoxide dismutase (SOD)-like活性；为确定苋菜的美白活性，酪氨酸酶抑制活性和黑色素含量进行了调查；为确 定苋菜的皱纹改善效果，弹性蛋白酶抑制活性和1型前胶原蛋白进行了测定。此外B16F10小鼠黑色素瘤细 胞和CCD986sk人真皮成纤维细胞用于检测细胞活力。结果: 在提取物浓度为500 μg/mL时，Amaranthus mangostanus ethanol extracts (AME)和Amaranthus mangostanus water extracts (AMW)的DPPH自由基 清除活性分别为49%和56%。AME和AMW的SOD-like活性分别为67%和57%，而标准样品L-抗坏血酸在1 mM 时，为38%。AME显示25.9% (L-酪氨酸)和17.8%(L-DOPA)，AMW显示23.4% (L-酪氨酸)和18.5% (L-DOPA) 的酪氨酸酶抑制测定。此外，在B16F10 小鼠黑色素瘤细胞内黑色素生成分别降低了25.5% (AME)和24.0% (AMW)。此外，弹性蛋白酶抑制测定结果表明AME (19.7%)和AMW (17.2%)在500 μg/mL时，具有抑制作用， 并且 I 型前胶原的量显示不显着。此外，当用100 μg/mL AME和AMW处理时，B16F10 小鼠黑色素瘤细胞的存 活率为分别为76%和86%。当我们分别用100 μg/mL AME和AMW处理时，CCD-986sk人真皮成纤维细胞的存活 率分别为94.5%和83.6%。结论: 这些结果表明，苋菜提取物具有优异的抗氧化、抗皱和美白效果。因此，它作 为化妆品或抗衰老相关生物制品的功能材料可能具有重要的工业价值。

목차

Abstract
Introduction
Methods
1. 실험 재료 및 시약
2. Anti-oxidant activity
3. Whitening effects
4. Anti-wrinkle effects
5. 세포독성시험
6. 통계처리
Results and Discussion
1. 항산화 활성
2. 미백 활성
3. 주름 개선 효과
4. Cell viability test
Conclusion
References
국문초록
中文摘要

키워드

Amaranthus mangostanus 항주름 화장품 참비름 미백 Amaranthus mangostamus Anti-wrinkle Cosmetic Chambirum Whitening 苋菜，抗皱，化妆品，Chambirum，美白

저자

• In Hae Kim [ 김인혜 | Department of Pharmaceutical Engineering, Silla University, Busan, Korea ]
• Jae Hwa Lee [ 이재화 | Department of Pharmaceutical Engineering, Silla University, Busan, Korea ] Corresponding author

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