Earticle

초록

영어

Background/Objectives: This study aims to demonstrate pro-inflammatory cytokine production inhibitory effects using Burdock extract. In addition, the basic mechanism of the anti-inflammatory mechanism of burdock is to be studied Methods/Statistical analysis: Thus, This paper was carried out to see the production of NO and pro-inflammatory cytokine ,TNF-α, IL-6 and IL-8 using the macrophages of LPS-treated mice. The cytotoxicity test was used 96 AQueous One solution cell proliferation assay. NO was measured using NO Detection Kit, and the production of pro-inflammatory cytokine was measured using ELISA kit. Findings: As a result, Burdock extract had no cytotoxicity at 10ug/mL to 1,000 ug/mL and significantly inhibited the production of TNF-α as well as IL-6 and IL-8 which are pro-inflammatory cytokines. In the RAW 264.7 macrophage cells, Pro-inflammatory cytokine production inhibitory effects are likely to be diversely utilized as basic physiological activity data and functional materials to demonstrate anti-inflammatory properties of broccoli extract. Improvements/Applications: In conclusion, this study can be used to a basic data to objectively demonstrate the physiological activity of immunological mechanism associated with the anti-inflammatory action of Burdock extract. However, in-depth research on anti-inflammatory is needed.

목차

Abstract
I. INTRODUCTION
II. MATERIALS AND METHOD
A. Experimental Material
B. RAW 264.7 Cell culture
C. Cytotoxicity
D. Measurement of NO
E. Effect of TNF-α, IL-6, IL-8
F. Statistical analysis
Ⅲ. RESULTS AND DISCUSSION
Ⅳ. Conclusion
REFERENCES

키워드

저자

• Jeong-Sook Park [ Department of Nursing, Nambu University, Gwangju, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  IJBSA

• 간기

  계간

• pISSN

  2619-8363

• 수록기간

  2019~2022

• 십진분류

  KDC 327 DDC 332