In this study, we analyzed the effect of media on the production of carotenoids and mycelia by using Perenniporia fraxinea. Malt extract-based medium with less than 0.1% peptone stimulated the production of carotenoids, and the one with more than 0.2% peptone inhibited its production. P. fraxinea grown in medium without malt extract did not produce carotenoids, although a small amount of peptone was added to the medium.After carotenoid production, the culture broth was separated using simple centrifugation and the supernatant was harvested as a carotenoid solution. Ethanol was used to extract carotenoids from mycelia. Carotenoid solution separated or extracted from the culture solution showed DPPH radical scavenging activity. The antioxidant carotenoids produced by P. fraxinea are derived from natural products, have no toxicity and side effects, and exhibit excellent antioxidant effects; therefore, they can be effectively used to remove oxides produced by active oxygen.
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ABSTRACT 서론 재료 및 방법 공시균주 배지 균사생장 속도 및 밀도 플라스크 배양 카르테노이드 분리 카르테노이드 분석방법 DPPH 라디컬 항산화 효과 측정 결과 및 토론 2차원 균사체 배양 특성 배지의 변화에 따른 카르테노이드 생산 맥아 추출물과 펩톤의 농도가 카르테노이드 생산에 미치는영향 맥아추출물과 포도당농도가 카르테노이드 생산에 미치는영향 공기부양식 배양기를 이용한 카르테노이드 생산 아까시재목버섯 유래 카르테노이드 항산화능 평가 적요 REFERENCES